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Search results for: 22kDa

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#31760664   2020/01/23 To Up

Photosystem II 22kDa protein level - a prerequisite for excess light-inducible memory, cross-tolerance to UV-C and regulation of electrical signalling.

It is well known that PsbS is a key protein for the proper management of excessive energy in plants. Plants without PsbS cannot trigger non-photochemical quenching, which is crucial for optimal photosynthesis under variable conditions. Our studies showed wild-type plants had enhanced tolerance to UV-C-induced cell death (CD) upon induction of light memory by a blue or red light. However, npq4-1 plants, which lack PsbS, as well as plants overexpressing this protein (oePsbS), responded differently. Untreated oePsbS appeared more tolerant to UV-C exposure, whereas npq4-1 was unable to adequately induce cross-tolerance to UV-C. Similarly, light memory induced by episodic blue or red light was differently deregulated in npq-4 and oePsbS, as indicated by transcriptomic analyses, measurements of the trans-thylakoid pH gradient, chlorophyll a fluorescence parameters, and measurements of foliar surface electrical potential. The mechanism of the foliar CD development seemed to be unaffected in the analysed plants and is associated with chloroplast breakdown. Our results suggest a novel, substantial role for PsbS as a regulator of chloroplast retrograde signalling for light memory, light acclimation, CD, and cross-tolerance to UV radiation.
Magdalena Górecka, Maria Lewandowska, Joanna Dąbrowska-Bronk, Maciej Białasek, Anna Barczak-Brzyżek, Milena Kulasek, Jakub Mielecki, Anna Kozłowska-Makulska, Piotr Gawroński, Stanisław Karpiński

1045 related Products with: Photosystem II 22kDa protein level - a prerequisite for excess light-inducible memory, cross-tolerance to UV-C and regulation of electrical signalling.

10ìg10 100ug Lyophilized100 0.1 mg500 100μg0.1 mg100ug Lyophilized200 ug100ug Lyophilized100ug Lyophilized

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#31702044   2019/10/01 To Up

Recombinant betatrophin (Angptl‑8/lipasin) ameliorates streptozotocin‑induced hyperglycemia and β‑cell destruction in neonatal rats.

Betatrophin [also known as lipasin, angiopoietin‑like 8 (ANGPTL8), refeeding induced in fat and liver (RIFL), or hepatocellular carcinoma‑associated gene TD26], a 22‑kDa protein in the angiopoietin‑like family, is a liver‑derived hormone that promotes pancreatic β‑cell proliferation and lipid metabolism. The aim of the present study was to investigate the effect of recombinant betatrophin on β‑cell regeneration in a neonatal streptozotocin (STZ)‑induced diabetic rat model. One‑day‑old Wistar rats were injected with STZ (100 mg/kg), followed by intraperitoneal administration of betatrophin to the STZ‑injected rats for 6 days. Plasma glucose and body weight were monitored. On days 4 and 7, expression levels of pancreatic duodenal homeobox gene‑1 (PDX‑1), the Bax/B‑cell lymphoma‑2 (Bcl‑2) ratio and plasma insulin were assessed, and the β‑cell proliferation rate was determined. Pancreatic islet area and number were determined at 10 weeks. It was found that betatrophin treatment alleviated STZ‑induced hyperglycemia, elevated pancreatic expression levels of Bcl‑2, PDX‑1, plasma insulin levels and the β‑cell proliferation rate on days 4 and 7. Long‑term betatrophin treatment improved glucose tolerance, associated with improved plasma insulin levels and β‑cell mass. These results suggest that early administration of betatrophin promotes β‑cell proliferation in STZ‑induced diabetic neonates and prevents the development of diabetes in adults.
Di Zhang, Yan-Juan Yu, Feng-Sen Xu, Jun-Hua Yuan, Rui Wang, Cai-Shun Zhang, Liu-Xin Wang, Yuan Liu, Li-Min Song, Jun-Li Liu, Jing Dong

1464 related Products with: Recombinant betatrophin (Angptl‑8/lipasin) ameliorates streptozotocin‑induced hyperglycemia and β‑cell destruction in neonatal rats.

96 assays1 mg1 mg400 ug10 ug400 ug1 mg5 mg10 1 mL

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#31676377   2019/10/29 To Up

N-terminal tau truncation in the pathogenesis of Alzheimer's disease (AD): Developing a novel diagnostic and therapeutic approach.

Tau truncation occurs at early stages during the development of human Alzheimer's disease (AD) and other tauopathy dementias. Tau cleavage, particularly in its N-terminal projection domain, is able to drive per se neurodegeneration, regardless of its pro-aggregative pathway(s) and in fragment(s)-dependent way. In this short review, we highlight the pathological relevance of the 20-22 kDa NH-truncated tau fragment which is endowed with potent neurotoxic "gain-of-function" action(s), both in vitro and in vivo. An extensive comment on its clinical value as novel progression/diagnostic biomarker and potential therapeutic target in the context of tau-mediated neurodegeneration is also provided.
G Amadoro, V Latina, V Corsetti, P Calissano

2929 related Products with: N-terminal tau truncation in the pathogenesis of Alzheimer's disease (AD): Developing a novel diagnostic and therapeutic approach.

96 tests96 tests

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#31356945   2019/07/26 To Up

Physicochemical and functional properties of a water-soluble polysaccharide extracted from Mung bean (Vigna radiate L.) and its antioxidant activity.

A water-soluble polysaccharide (MBP-1) was isolated from Mung bean (Vigna radiate L.) and its physicochemical characterization, functional properties, and antioxidant activity was investigated. Chemical analysis revealed that the contents of carbohydrate, uronic acid, and protein were 33.05 ± 0.10%, 25.83 ± 0.04%, and 3.17 ± 0.36%, respectively. The molecular weight (Mw) of MBP-1 was 146.22 kDa and it was consist of arabinose (Ara), glucose (Glc), xylose (Xyl), galactose (Gal), and galacturonic acid (GlcA) in a molar ratio of 0.94:0.37:0.33:0.13:5.99. The results of X-ray diffractometry (XRD) and thermal gravimetric analysis (TGA) indicated MBP-1 was semi-crystalline material with better thermal stability. MBP-1 exhibited good hydration properties and oil holding capacity. In addition, the antioxidant assay results showed MBP-1 had high 1,1-Diphenyl-2-picrylhydrazylradical (DPPH) and hydroxyl radical scavenging activities with concentration for 50% of maximal effect (EC50) of 1.59 and 0.51 mg/mL. Overall, these results indicated that MBP-1 presents a promising natural source of food additive or antioxidant.
Qianqian Song, Lian Jiang, Xinquan Yang, Lixin Huang, Yue Yu, Qiang Yu, Yi Chen, Jianhua Xie

1795 related Products with: Physicochemical and functional properties of a water-soluble polysaccharide extracted from Mung bean (Vigna radiate L.) and its antioxidant activity.

100ug1,000 tests50 ug 1 mg1000 TESTS/0.65ml25 mg1000 tests2.5 mg200ug10 mg

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#31351952   2019/07/25 To Up

Biochemical, biological characteristics and antibacterial activity of glycoprotein extracted from the epidermal mucus of African catfish (Clarias gariepinus).

Catfish glycoprotein (CFG) was extracted from the cutaneous mucus of Egyptian catfish by ammonium sulphate precipitation and purified on gel filtration column (sephadex G-50). After purification, CFG produced one band on SDS-PAGE (22 kDa). Urea-PAGE and the pH-solubility of CFG indicated its positive charge (IEP 8). CFG contained 12 saccharides. FTIR spectrum shows 3 groups of bands at 1800-2900, 1100-1700 and 700-1100 cm. CFG exhibited antibacterial activity against 9 pathogenic bacteria with low MIC (50 μg/mL), where two Gram+ bacteria, i.e.; Streptococcus pyogenes (St. pyogenes) and Listeria ivanovii (L. ivanovii) were the most sensitive. The growth curves of the bacteria subjected to 1 MIC of CFG during 30 h showed general growth inhibition, particularly in case of Gram bacteria such as E. coli. TEM images showed evidently reduced relative content of the intact cells and clear incurred cellular malformations. Combining CFG with specific antibiotic at equal ratios induced synergistic antibacterial actions, amounting to 40% of the mathematical sum of the combination. Substituting the antibiotic chloramphenicol with gradual increasing ratios of CFG of its starting concentration (30 μg/mL), produced proportionally bigger antibacterial actions against St. pyogenes growth and increasing synergistic effect up to 37% at 80% of CFG substitution.
Seham Abdel-Shafi, Ali Osman, Abdul-Raouf Al-Mohammadi, Gamal Enan, Nehal Kamal, Mahmoud Sitohy

1984 related Products with: Biochemical, biological characteristics and antibacterial activity of glycoprotein extracted from the epidermal mucus of African catfish (Clarias gariepinus).

5 G50 ug10 ug1x96 well platemin 2 cartons100ug Lyophilized 100 G50 ug 25 mg 96 Tests

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#31284006   2019/07/05 To Up

High-pressure ultrasonic-assisted extraction of polysaccharides from Hovenia dulcis: Extraction, structure, antioxidant activity and hypoglycemic.

In this study, high-pressure ultrasonic-assisted extraction (HUE) technology was used for polysaccharide extraction from Hovenia dulcis (HDPS). A maximal extraction yield (11.81 ± 0.26%) was obtained using the response surface method (RSM) with optimized HUE conditions (Ultrasound power 330 W, 68 °C, and 22 min). Results showed that HDPS mainly contained neutral sugar (47.81 ± 1.56%), uronic acid (12.52 ± 0.15%), and protein (3.64 ± 0.24%), specifically. HDPS was comprised of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose in a molar ratio of 3.20%, 20.40%, 4.81%, 11.14%, 27.37% and 33.08%, respectively. And with an average molecular weight of 185.22 kDa for its major component (accounting for 90.13% for HDPS). The result obtained from the X-ray diffraction pattern confirmed a semi-crystalline nature of the sample. HUE-HDPS suggested typical shear thinning behavior and non-Newtonian properties. Additionally, HUE-HDPS exhibited better chemical antioxidant activities in DPPH, ABTS, hydroxyl radical scavenging and Fe chelation activity. The in-vitro and in-vivo experiments indicated that HUE-HDPS had obviously hypoglycemic effects. Accordingly, the polysaccharides from Hovenia dulcis might serve as a natural antioxidant and hypoglycemic agent.
Bing Yang, Qunjun Wu, Yuxin Luo, Qiong Yang, Xunyu Wei, Jianquan Kan

1124 related Products with: High-pressure ultrasonic-assisted extraction of polysaccharides from Hovenia dulcis: Extraction, structure, antioxidant activity and hypoglycemic.

1 kit100 extractions100 extractionsProteinase K Powder, 2 ea50 assays100 assays 150 extractions50 preps100 extractions

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#30733738   2018/05/26 To Up

Purification and characterization of alkaline protease with novel properties from strain S8.

Proteases are the hydrolytic enzymes which hydrolyzes peptide bond between proteins with paramount applications in pharmaceutical and industrial sector. Therefore production of proteases with efficient characteristics of biotechnological interest from novel strain is significant. Hence, in this study, an alkaline serine protease produced by strain S8 (MTCC NO 11901) was purified and characterized. The alkaline protease was purified by ammonium sulfate precipitation (50%), ion exchange (DEAE-Cellulose) and gel filtration (Sephadex G-100) chromatographic techniques. As a result of this purification, a protein with specific activity of 300U/mg protein was obtained with purification fold 17.04 and recovery percentage of 34.6%. The molecular weight of the purified protease was determined using SDS-PAGE under non-reducing (71 kDa) and reducing conditions (35 kDa and 22 kDa). Zymogram analysis revealed that proteolytic activity was only associated with 22 kDa. These results indicate that existence of the enzyme as dimer in its native state. The molecular weight of the protease (22 kDa) was also determined by gel filtration (Sephadex G-200) chromatography and it was calculated as 21.8 kDa. The optimum activity of the protease was observed at pH 10.0 and temperature 70 °C with great stability towards pH and temperature with casein as a specific substrate. The enzyme was completely inhibited by PMSF and TLCK indicating that it is a serine protease of trypsin type. The enzyme exhibits a great stability towards organic solvents, oxidizing and bleaching agents and it is negatively influenced by Li and Co metal ions. The purified protein was further characterized by Matrix Assisted Laser Desorption Ionization/Mass Spectroscopy (MALDI/MS) analysis which reveals that total number of amino acids is 208 with isoelectric point 9.52.
B K M Lakshmi, D Muni Kumar, K P J Hemalatha

2493 related Products with: Purification and characterization of alkaline protease with novel properties from strain S8.

100ml100ug50gm50 Pieces/case 5 G 100ul5 sets1 ml 8 ml 96tests100ul100ug Lyophilized

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#30633923   2019/01/08 To Up

Expression of growth hormone and growth hormone receptor genes in human eye tissues.

In humans, the polygenic growth hormone (GH) locus is located on chromosome 17 and contributes with three types of proteins: pituitary GH which consists of at least two isoforms one of 22 kDa and the other of 20 kDa, placental GH, which also exhibits isoforms, and chorionic somatomammotropin hormone (CSH). While pituitary GH results from the expression of the GH-1 (GH-N) gene, placental GH is produced by the expression of the GH-2 (GH-V) gene and CSH is contributed by expression of the CSH-1 and CSH-2 genes. The location where GH-1 is expressed is the anterior pituitary and the rest of the genes in the locus are expressed in placenta. On the other hand, expression and synthesis of GH in extra-pituitary tissues, including the eye, has been recently described. However, the physiological role of GH in the eye has not yet been elucidated, although a possible neuroprotective role has been hypothesized. Thus, we analyzed GH-1, GH-2, CSH1/2, Pit-1, GHR, GHRH, GHRHR, SST, SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 to elucidate the expression and regulation of the GH locus in the human eye. Through qPCR analysis, we only found evidence of GH-1 expression in retina, choroid and trabecular meshwork; its transcript turned out to be the same as pituitary GH mRNA found in major species, and no splicing variants were detected. PIT1 was absent in all the ocular tissues implying an independent GH-1 expression mechanism. We found evidence of GHR in the cornea, choroid coat and retina. These results suggest autocrine and/or paracrine regulation, possibly exerted by GHRH and SSTs (since their mRNAs and receptors were found predominantly in retinal, choroidal and corneal tissues) since expression of both molecules was detected in different ocular tissues, as well as in the same tissues where GH-1 expression was confirmed. Our results add solid evidence about the existence of a regulatory local system for GH expression and release in the human eye.
Diana Cristina Pérez-Ibave, María Lourdes Garza-Rodríguez, Antonio Alí Pérez-Maya, Irám Pablo Rodríguez-Sánchez, Maricela Luna-Muñoz, Carlos Guillermo Martínez-Moreno, Carlos Arámburo-de la Hoz, Jibran Mohamed-Noriega, Karim Mohamed-Noriega, Jesús Mohamed-Hamsho, Víctor Manuel Bautista-De Lucío, Hugo Alberto Barrera-Saldaña

1543 related Products with: Expression of growth hormone and growth hormone receptor genes in human eye tissues.

100.00 ug0.2 mg1 mg1mg x 20100ug5 x 50 ug1 mg96 wells (1 kit)5 mg50 ug1 mg500 ìg

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#30396395   2018/09/28 To Up

Xylanase from Aspergillus tamarii shows different kinetic parameters and substrate specificity in the presence of ferulic acid.

A 22 kDa xylanase (AtXyl1) from Aspergillus tamarii was purified by two chromatographic steps and presented preference for oat spelt (OSX), birchwood (BrX) and beechwood (BeX) xylans respectively, as substrates. AtXyl1 displays the highest activity at pH 5.5 and 55 °C and showed tolerance over a range of different phenolic compounds. The activity of AtXyl1 was not inhibited when the enzyme was incubated with ferulic acid (FA) using OSX or BrX as substrate. On the other hand, the incubation of AtXyl1 with BeX and FA resulted in an increase in enzyme activity. The molecular docking of a GH11 xylanase from Aspergillus niger with FA showed the preference for binding within the catalytic site. The position of FA was based on the presence or absence of a complexed substrate. When the enzyme from A. niger was docked in the absence of xylan in its crystal structure, FA interacted with Tyr164 and a water molecule. For the enzyme socked with xylo-oligosaccharides, FA interacted with Ser94, Tyr89 and the xylo-oligosaccharide present in the catalytic site. Thermodynamic parameters from the reaction of AtXyl1 with different xylans and FA indicate that FA can cause a conformational change in the enzyme, and this can influence the substrate fitting and makes the enzyme tolerant or active toward the substrate. Our findings suggest that enzyme activation or tolerance to phenolic compounds can be correlated to subtle changes in enzyme conformation due to the presence of the phenolic compound.
Antonielle Vieira Monclaro, Guilherme Lima Recalde, Francides Gomes da Silva, Sonia Maria de Freitas, Edivaldo Ximenes Ferreira Filho

2880 related Products with: Xylanase from Aspergillus tamarii shows different kinetic parameters and substrate specificity in the presence of ferulic acid.

10 ug10 mg100ug Lyophilized100ug Lyophilized 500 G1 g1 g

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#30171943   2018/08/29 To Up

Structural and functional analyses of three purified polysaccharides isolated from Chinese Huaishan-yams.

The extraction, purification and structural of Chinese Huaishan-yams polysaccharides and their function were investigated in this study. Three water-soluble polysaccharides, designated as CYZ, CYS-1, CYS-2, were purified and the molecular weight were found to be 22 KDa, 41 KDa and 23 KDa, respectively. The HPLC results showed that they were composed of different monosaccharides and the content of monosaccharides varied significantly. H NMR and C NMR spectra showed that CYS-1 contained two α-type and four β-type glycosidic linkages and backbone of CYS-1 has two types of linkages, β-1 → 2 and β-1 → 4. CYS-2 contained two α type and three β-type glycosidic linkages, and the backbone has three types of linkages, β-1 → 2, β-1 → 4, and β-1 → 6. The antioxidant activities increased in the order of CYS-1 < CYZ < CYS-2 < crude polysaccharide. Further, crude polysaccharide CYS-1 (≥80 μg/mL) had a good ability of inhibiting α-glucosidase and significant inhibition on the relative proliferation rate of B16 murine melanoma cells middle dose (≥200 μg/mL).
Yunping Zhu, Li Yang, Chengnan Zhang, Yulian Tian, Feifei Zhang, Xiuting Li

1245 related Products with: Structural and functional analyses of three purified polysaccharides isolated from Chinese Huaishan-yams.

100μl100ug100ul100μl100μl100μl1 kit(96 Wells)100μl100μl100μl1000 TESTS/0.65ml100μl

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