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Search results for: 3-Amino-5-[[[2,3-bis(acetyloxy)propyl]amino]carbonyl]-2,4,6-triiodo-benzoic Acid C15H15I3N2O7 CAS: 122731-59-3

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#32246713   2020/04/04 To Up

PAM recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage.

In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer unprecedented flexibility, however, more compact versions would simplify delivery and extend application. Here, we present a collection of 10 exceptionally compact (422-603 amino acids) CRISPR-Cas12f nucleases that recognize and cleave dsDNA in a PAM dependent manner. Categorized as class 2 type V-F, they originate from the previously identified Cas14 family and distantly related type V-U3 Cas proteins found in bacteria. Using biochemical methods, we demonstrate that a 5' T- or C-rich PAM sequence triggers dsDNA target cleavage. Based on this discovery, we evaluated whether they can protect against invading dsDNA in Escherichia coli and find that some but not all can. Altogether, our findings show that miniature Cas12f nucleases can protect against invading dsDNA like much larger class 2 CRISPR effectors and have the potential to be harnessed as programmable nucleases for genome editing.
Tautvydas Karvelis, Greta Bigelyte, Joshua K Young, Zhenglin Hou, Rimante Zedaveinyte, Karolina Budre, Sushmitha Paulraj, Vesna Djukanovic, Stephen Gasior, Arunas Silanskas, Česlovas Venclovas, Virginijus Siksnys

1730 related Products with: PAM recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage.

50ul (1mg/ml)50ul (1mg/ml)50ul (1mg/ml)50ul (1mg/ml)50ul0.1ml (1mg/ml)1.0ml0.1ml1mg0.1ml (1mg/ml)100ug Lyophilized250ul

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#32245800   2020/04/03 To Up

Glucose-Stimulated Insulin Secretion Fundamentally Requires HO Signaling by NADPH Oxidase 4.

NADPH facilitates glucose-stimulated insulin secretion (GSIS) in pancreatic islet (PI) β-cells by an as yet unknown mechanism. We found NADPH oxidase, isoform-4 (NOX4), to be the major producer of cytosolic HO, essential for GSIS, while the increase in ATP/ADP alone was insufficient. The fast GSIS phase was absent in PIs from NOX4-null, β-cell-specific knockout mice (NOX4βKO) (not NOX2KO), and NOX4-silenced or catalase-overexpressing INS-1E cells. Lentiviral NOX4 overexpression or HO rescued GSIS in PIs from NOX4βKO mice. NOX4 silencing suppressed Ca oscillations and the patch-clamped ATP-sensitive potassium channel (K) opened more frequently at high glucose. Mitochondrial HO, decreasing upon GSIS, provided an alternative redox signaling when 2-oxo-isocaproate or fatty acid oxidation formed superoxide by electron-transport flavoprotein:Q-oxidoreductase. Unlike GSIS, this ceased with mitochondrial antioxidant SkQ1. Both NOX4KO and NOX4βKO strains exhibited impaired glucose tolerance and peripheral insulin resistance. Thus the redox signaling previously suggested to cause β-cell-self-checking - hypothetically induces insulin resistance when absent. In conclusion, ATP plus HO elevations constitute an essential switch-on signal of insulin exocytosis for glucose and branched-chain oxoacids as secretagogues (partly for fatty acids). Redox signaling could be impaired by cytosolic antioxidants, hence those targeting mitochondria should be preferred for clinical applications to treat (pre)diabetes at any stage.
Lydie Plecitá-Hlavatá, Martin Jabůrek, Blanka Holendová, Jan Tauber, Vojtěch Pavluch, Zuzana Berková, Monika Cahová, Katrin Schroeder, Ralf P Brandes, Detlef Siemen, Petr Ježek

1353 related Products with: Glucose-Stimulated Insulin Secretion Fundamentally Requires HO Signaling by NADPH Oxidase 4.

96 assays200ul2 Pieces/Box2 ml200ul25000 units1 ml200ul2 Pieces/Box250/kit0.1ml (1mg/ml)1 kit

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#32243843   2020/04/01 To Up

Mitoregulin Controls β-Oxidation in Human and Mouse Adipocytes.

We previously discovered in mouse adipocytes an lncRNA (the homolog of human LINC00116) regulating adipogenesis that contains a highly conserved coding region. Here, we show human protein expression of a peptide within LINC00116, and demonstrate that this peptide modulates triglyceride clearance in human adipocytes by regulating lipolysis and mitochondrial β-oxidation. This gene has previously been identified as mitoregulin (MTLN). We conclude that MTLN has a regulatory role in adipocyte metabolism as demonstrated by systemic lipid phenotypes in knockout mice. We also assert its adipocyte-autonomous phenotypes in both isolated murine adipocytes as well as human stem cell-derived adipocytes. MTLN directly interacts with the β subunit of the mitochondrial trifunctional protein, an enzyme critical in the β-oxidation of long-chain fatty acids. Our human and murine models contend that MTLN could be an avenue for further therapeutic research, albeit not without caveats, for example, by promoting white adipocyte triglyceride clearance in obese subjects.
Max Friesen, Curtis R Warren, Haojie Yu, Takafumi Toyohara, Qiurong Ding, Mary H C Florido, Carolyn Sayre, Benjamin D Pope, Loyal A Goff, John L Rinn, Chad A Cowan

1543 related Products with: Mitoregulin Controls β-Oxidation in Human and Mouse Adipocytes.

100 μg100 μg100 μg100 μg100 μg100 μg500 100uL100ug Lyophilized25 200 100.00 ug

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#32243147   2020/04/03 To Up

The potential of competitive antagonists of insect ionotropic GABA receptors as insecticides.

Ionotropic γ-aminobutyric acid (GABA) receptors (GABARs) represent an important insecticide target. Currently used GABAR-targeting insecticides are noncompetitive antagonists (NCAs) of these receptors. Recent studies have demonstrated that competitive antagonists (CAs) of GABARs have functions of inhibiting insect GABARs similar to NCAs and that they also exhibit insecticidal activity. CAs have different binding sites and different mechanisms of action compared with those of NCAs. Therefore, GABAR CAs should have the potential to be developed into novel insecticides, which could be used to overcome the developed resistance of insect pests to conventional NCA insecticides. Although research on insect GABAR CAs has lagged behind that on mammalian GABAR CAs, research on the CAs of insect ionotropic GABARs has made great progress in recent years, and several series of heterocyclic compounds, such as 3-isoxazolols and 6-iminopyridazines, have been identified as insect GABAR CAs. In this review, we briefly summarize the design strategies, structures, and biological activities of the novel GABAR CAs that have been found in the last decade. Updated information about GABAR CAs may benefit the design and development of novel GABAR-targeting insecticides.
Genyan Liu, Yun Wu, Ya Gao, Xiu-Lian Ju, Yoshihisa Ozoe

2919 related Products with: The potential of competitive antagonists of insect ionotropic GABA receptors as insecticides.

1 kit1 kit100 plates1 kit10 plates1 kit100 plates1 kit 5 G10 plates100ug Lyophilized100ul

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#32242881   2020/04/03 To Up

Combating metastasis of breast cancer cells with a carboplatin analogue containing an all-trans retinoic acid ligand.

Pt-ATRA, a carboplatin analogue containing an all-trans retinoic acid (ATRA) derivative ligand, was synthesized via a click reaction. Upon cellular internalization, Pt-ATRA exhibits a dual function, releasing an active Pt(ii) moiety to induce cell apoptosis and ATRA to inhibit tumor metastasis.
Yi Dai, Hai Huang, Yang Zhu, Junjie Cheng, Ai-Zong Shen, Yangzhong Liu

1104 related Products with: Combating metastasis of breast cancer cells with a carboplatin analogue containing an all-trans retinoic acid ligand.

100 ug/vial96T100ul50 100 µl100ul

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#32242474   2020/04/03 To Up

Long Term Response to a Bioactive Biphasic Biomaterial in the Femoral Neck of Osteoporotic Rats.

Osteoporosis often leads to fragility fractures of the hip resulting in impaired quality of life and increased mortality. Augmenting the proximal femur could be an attractive option for prevention of fracture or fixation device failure. We describe a tissue engineering based strategy to enhance long-term bone formation in the femoral neck of osteoporotic rats by locally delivering bioactive molecules; bone morphogenic protein-2 (rhBMP-2) and zoledronic acid (ZA) using a calcium sulphate/hydroxyapatite (CaS/HA) biomaterial. A defect was created by reaming the femoral neck canal of osteoporotic (OVX) rats and they were treated as follows: G1. Empty, G2. CaS/HA, G3. CaS/HA + Systemic ZA, G4. CaS/HA + Local ZA and G5. CaS/HA + Local ZA + rhBMP-2. Bone formation was evaluated 6 months after treatment. Furthermore, radioactively labelled 14C-ZA was used to study the bioavailability of ZA at the defect location, which was determined using scintillation counting. Micro-CT indicated significantly higher bone volume (BV) in groups G4 and G5 compared to the other treatment groups. This was confirmed qualitatively by histological assessment. Addition of rhBMP-2 gave no additional benefit in this model. Local delivery of ZA performed better than systemic administration of ZA. Mechanical testing showed no differences between the groups, likely reflecting that the addition of bioactive molecules had limited effect on cortical bone or the choice of mechanical testing setup was not optimal. Scintillation counting revealed higher amounts of 14C-ZA present in the treated leg of G4 compared to its contralateral control and compared to G3 indicating that local ZA delivery can be used to achieve high local concentrations without causing a systemic effect. This long-term study shows that local delivery of ZA using a CaS/HA carrier can regenerate cancellous bone in the femoral neck canal and has clear implications for enhancing implant integration and fixation in fragile bone.
Deepak Bushan Raina, Aurimas Sirka, Irfan Qayoom, Arun Kumar Teotia, Yang Liu, Sarunas Tarasevicius, K Elizabeth Tanner, Hanna Isaksson, Ashok Kumar, Magnus Tägil, Lars Lidgren

2541 related Products with: Long Term Response to a Bioactive Biphasic Biomaterial in the Femoral Neck of Osteoporotic Rats.

0.1 mg 100.00 ug100 μg100 μg100 μg

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#32242063   2020/04/02 To Up

Accurate and rapid discrimination of cigarette and household decoration material ash residues by negative chemical ionization TOFMS via acid-enhanced evaporation.

The detection and identification of cigarette ash in fire debris can be meaningful in fire investigations caused by burning cigarettes. In this work, a novel analytical method based on negative chemical ionization time-of-flight mass spectrometry (NCI/TOFMS) combined with a phosphoric-acid-enhanced evaporation strategy has been developed for the discrimination of cigarette ash samples (CAs) and common household decoration material ash samples (CHDMAs). A series of characteristic ions representing the acidified products HNCO and formic acid in the CAs were achieved, whose signal responses were enhanced with the help of mechanical agitation operation. To account for both the signal responses of the characteristic ions and acid corrosion of the ion source, the dynamic-purge gas was chosen to be 200 mL/min. The whole time for analysis was only 5 min, which is suitable for high-throughput measurements of large quantities of fire debris. As a result, a preliminary discrimination was achieved between the CAs and CHDMAs by virtue of the chemometric tool of principal components analysis (PCA) based on intensity differences of the characteristic ions. The results are encouraging and highlight the potential of NCI/TOFMS without complicated sample preparation steps for the accurate and high-throughput identification of cigarette ash on substrates in fire debris.
Shujun Liu, Yuanyuan Xie, Ximing Song

2437 related Products with: Accurate and rapid discrimination of cigarette and household decoration material ash residues by negative chemical ionization TOFMS via acid-enhanced evaporation.

10 mg500 MG100ug 5 G2.5 mg50 mg100ug1,000 tests200ug100ug50 ug

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#32239414   2020/04/02 To Up

Chemical characterization of mountain forest soils: impact of long-term atmospheric deposition loadings (Czech-Polish-German border region).

The composition of lipids in soil offers clues to soil degradation processes due their persistency and selectivity in soil, and close relation to long-term processes in the ecosystem, thanks to their role in cell membranes of organisms. Organic solvent-extractable compounds were recovered from soils collected at two sites differing in the degree of forest damage. Gas chromatography/mass spectroscopy and Fourier transform infrared spectroscopy were applied in order to characterize solvent-extractable lipids. Raman spectroscopy was also applied as it provides distinct advantages for determining the structural order of carbonaceous materials. The organic matter measurement techniques were combined with an established simultaneous multi-element measurement technique. Variations in individual soil horizons from the sites were reflected in the crystallinity of epicuticular waxes, presence of long-chain aliphatic hydrocarbons, concentrations of n-alkanes, saturated and unsaturated fatty acids, dicarboxylic acids, and in the content of aromatic structures, hydroxyl, ester, and carboxylic acid groups. The results are explained by differently transformed organic matter. The concentrations of elements in the soils were also affected by atmospheric depositions, including higher accumulations of arsenic and antimony, and lower contents of natural nutrients. These data have potential to be used as sensitive biogenic indicators of ecosystem damage by long-term atmospheric depositions.
Martina Havelcová, Vladimír Machovič, František Novák, Ladislav Lapčák, Jiří Mizera, Jiří Hendrych

2701 related Products with: Chemical characterization of mountain forest soils: impact of long-term atmospheric deposition loadings (Czech-Polish-German border region).

100ul100 μg100 μg100 μg100 μg100 μg100 μg100ul100 μg100 μg100 μg100 μg

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#32238231   2020/04/02 To Up

sp. nov., isolated from Blume.

A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004, was isolated from the tubers of Blume collected from Yunnan Province, PR China. The 16S rRNA gene sequence result showed that the strain SYSUP0004 shared low similarity (97.7 %) with the type strain of . SYSUP0004 grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4 with an interpeptide bridge l-ornithine-d-glutamic acid. Cell-wall sugars were mannose, ribose, glucose, galactose and fucose. The menaquinone was MK-9(H). The major fatty acids were anteiso-C anteiso-C A, C and anteiso-C. The polar lipids of SYSUP0004 were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid. The genomic DNA G+C content was 76.5 %. The average nucleotide identity values between SYSUP0004 and members of the genus were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity. Thus, based on the above results strain SYSUP0004 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain, SYSUP0004 (=KCTC 49025=CGMCC 1.16405).
Yan-Qiong Li, Hui Zhang, Min Xiao, Zhou-Yan Dong, Jing-Yi Zhang, Manik Prabhu Narsing Rao, Wen-Jun Li

2016 related Products with: sp. nov., isolated from Blume.

50 ul100 ug100 ul100 ul50 ul

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#32235626   2020/03/30 To Up

In Vitro Antibacterial and Antiproliferative Potential of Mattf. (Asteraceae) and Identification of Potential Bioactive Compounds.

Many species belonging to the genus are widely used in traditional medicine to treat infectious diseases and cancers. The present study aimed to evaluate the antibacterial and antiproliferative properties of Mattf. (Asteraceae). The activity of the methanolic extract and subsequent partition fractions was investigated against drug-resistant bacteria (Gram-negative and Gram-positive) and human tumor cell lines using broth microdilution and sulforhodamine B (SRB) assay, respectively. Our findings revealed weak to moderate antibacterial activities of tested extracts, with the recorded minimal inhibitory concentrations ranging from 256 to 1024 µg/mL. The ethyl acetate fraction (EL-EA) was found to be the most effective. Likewise, that fraction displayed strong antiproliferative potential with recorded IC of 8.27 µg/mL and 28.27 µg/mL on A549 and HeLa cells, respectively. An analysis based on the ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) of the EL-EA fraction allowed the identification of 32 compounds, of which quinic acid and derivatives, cinnamic acid derivatives, dihydrokaempferol, naringenin-7--glucoside, apigenin-7--d-glucoside, naringin, apigenin, rhoifolin, coniferyl aldehyde, and secoisolariciresinol are well-known compounds of biological importance. This study is first to report on the biological activity and phytochemical profile of . We provide a baseline to consider as a valuable source of anti-infective and antiproliferative agents.
Armel Jackson Seukep, Yong-Li Zhang, Yong-Bing Xu, Ming-Quan Guo

1566 related Products with: In Vitro Antibacterial and Antiproliferative Potential of Mattf. (Asteraceae) and Identification of Potential Bioactive Compounds.

25 mg1 kit10 mg100ug50 ug 500 MG25 mg96 wells96T100ul50 mg1000 tests

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