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Search results for: 3β-O-Acetyl-androsta-5,15-dien-17-one C21H28O3 CAS: 17921-64-1

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#33984647   2021/04/26 To Up

Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography.

Cardiolipins (1,3-bis(sn-3'-phosphatidyl)-sn-glycerol) (CLs) are widespread in many organisms, from bacteria to higher green plants and mammals. CLs were observed in Gram-positive bacterium of the genus Kocuria, brewer's yeast Saccharomyces, the green alga Chlamydomonas, spinach and beef heart. A mixture of molecular species of CLs was obtained from total lipids by hydrophilic interaction liquid chromatography (HILIC), and these were further separated and identified by reversed phase LC/MS with negative tandem electrospray ionization. The majority of CLs molecular species from each organism were cleaved using phospholipase C from Bacillus cereus. This phospholipase cleaves CLs into 1,2-diglycerols and phosphatidylglycerol 3-phosphates, which were then separated. After CLs cleavage, diacylglycerols such as sn-1,2-diacyl-3-acetyl-glycerols (i.e., triacylglycerols) were separated and identified by chiral chromatography/MS-positive tandem ESI. Significant differences in the composition of the molecular species between the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of CLs were found in all organisms tested. Molecular species of CLs that contained four different fatty acids were identified in all five samples, and CLs containing very long chain fatty acids were identified in yeast. In addition, CLs containing both enantiomers (at the sn-2 carbon) were present in the bacterium tested. These findings were further supported by data already published in GenBank where, in the same family - Micrococcaceae - both enzymes responsible for chirality in the sn-2 position, glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases, were present.
Milada Vítová, Milena Stránská, Andrea Palyzová, Tomáš Řezanka

1782 related Products with: Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography.

10 ug100ul100 assays 5 G 5 G25 mg50 ul0.2 mg 5 G96 wells (1 kit)25 assays

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#33984629   2021/05/10 To Up

Functional brain networks underlying automatic and controlled handwriting in Chinese.

This study aimed to identify the functional brain networks underlying the distinctions between automatic and controlled handwriting in Chinese. Network-based analysis was applied to functional magnetic resonance imaging data collected while adult participants performed a copying task under automatic and speed-controlled conditions. We found significant differences between automatic and speed-controlled handwriting in functional connectivity within and between the frontoparietal network, default mode network, dorsal attention network, somatomotor network and visual network; these differences reflect the variations in general attentional control and task-relevant visuomotor operations. However, no differences in brain activation were detected between the two handwriting conditions, suggesting that the reorganization of functional networks, rather than the modulation of local brain activation, underlies the dissociations between automatic and controlled handwriting in Chinese. Our findings illustrate the brain basis of handwriting automaticity, shedding new light on how handwriting automaticity may be disrupted in individuals with neurological disorders.
Junjun Li, Lei Hong, Hong-Yan Bi, Yang Yang

2565 related Products with: Functional brain networks underlying automatic and controlled handwriting in Chinese.

96 tests50ug96 tests100 μg96 tests

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#33984618   2021/05/09 To Up

Convergent validity of the Chinese version of the Multidimensional Schizotypy Scale.

Schizotypy is the latent personality reflecting the liability to schizophrenia. The Multidimensional Schizotypy Scale (MSS) is a newly developed questionnaire to measure the levels of schizotypy. The Chinese version of MSS has been developed and previous findings supported its structure validity. The present study aimed to examine the construct validity of the Chinese version of the MSS by correlating it with the Schizotypal Personality Questionnaire (SPQ). A total of 1359 university students completed the MSS and the SPQ online. The results of 1027 valid participants demonstrated that all MSS dimensions showed good internal consistency. The MSS positive dimension is strongly correlated with SPQ cognitive-perceptual factor, the MSS negative dimension with the SPQ interpersonal factor, and the MSS disorganized dimension with the SPQ disorganized factor. Taken together, our study provides evidence for construct validity of the Chinese version of the MSS.
Ya Wang, Hui-Xin Hu, Ling-Ling Wang, Yi-Jing Zhang, Han-Xue Yang, Han-Yu Zhou, Xu Li, Simon S Y Lui, Raymond C K Chan

1651 related Products with: Convergent validity of the Chinese version of the Multidimensional Schizotypy Scale.

500IU1min 2 cartons100.00 ul200 units11 ml500 Units

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#33984564   2021/04/20 To Up

Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein.

β-Cryptoxanthin (BCX) possesses potential therapeutic and health benefits. However, BCX absorption is low because of its poor aqueous solubility. In this study, a complex between BCX and casein (Cas) was prepared to improve the water dispersibility and bioavailability of BCX. BCX was recovered quantitatively from freeze-dried kumquat powder through solid-liquid extraction and saponification. The complexation significantly improved the apparent solubility of BCX under acidic and neutral conditions. A cell membrane permeation test using a Caco-2 cell monolayer was performed to evaluate the bioavailability of the BCX-Cas complex. This complex and a blank sample were digested in vitro and added to the apical side of the Caco-2 cell membrane. The quantity of BCX that permeated using the BCX-Cas complex after 24 h was 22.7 times greater than that of the blank. Thus, complexation of BCX with Cas improved dramatically the bioavailability of BCX from a kumquat extract.
Tatsuya Oshima, Koki Takahashi, Asuka Inada, Masao Yamasaki, Yumi Yamasaki, Nozomu Eto

2537 related Products with: Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein.

100ug Lyophilized100 ul100ug Lyophilized2500 assays200ug 2x5L200ul200ug10 mg2500 Tests

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#33984436   2021/05/10 To Up

New sterols from the South China Sea sponges Halichondria sp.

A detailed chemical investigation of two specimen of South China Sea sponges Halichondria sp. (No. 19-XD-47 and No. 17-XD-87) have resulted in the isolation of three new sterols, namely, halichsterols A (1), B (2) and C (3), together with seven related known ones (4-10). Their structures were determined by extensive spectroscopic analysis and by comparison with the spectral data reported in the literature. In bioassay, compound 2 displayed significantly anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells.
Bao Chen, Wang-Sheng Li, Yu-Cheng Gu, Hai-Yan Zhang, Hui Luo, Chang-Yun Wang, Yue-Wei Guo

2468 related Products with: New sterols from the South China Sea sponges Halichondria sp.

125 ml 10500 ml1000.1 mg 100 G1100 IU500 Units1mg

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#33984382   2021/05/10 To Up

Prolonged release and shelf-life of anticoagulant sulfated polysaccharides encapsulated with ZIF-8.

Natural active polysaccharides are attracting increased attention from pharmaceutical industries for their valuable biological activities. However, the application of polysaccharides has been restricted due to their relatively large molecular weight, complex structure, and instability. Metal-organic frameworks (MOFs) have emerged to help deliver cargo to specific locations, achieving the objectives of eliminating the potential damage to the body, protecting the drugs, and improving therapeutic effectiveness. Here, a pH-responsive zeolitic imidazolate framework (ZIF-8) was synthesized to encapsulated three sulfated polysaccharides (heparin, fucan sulfate, fucosylated chondroitin sulfate) and a non-sulfated polysaccharide, hyaluronic acid. The resulting [email protected] biocomposites showed differences in terms of morphology, particle size, encapsulation, and release efficiency. These biocomposites retained antithrombotic activity and the framework ZIF-8 effectively protected these polysaccharides from degradation and prolonged shelf-life of the anticoagulants from the unfavorable environment.
Jie Zheng, Bingzhi Li, Yuan Ji, Yin Chen, Xun Lv, Xing Zhang, Robert J Linhardt

2118 related Products with: Prolonged release and shelf-life of anticoagulant sulfated polysaccharides encapsulated with ZIF-8.

100tests100tests250tests100tests100tests10 mg100ug5/100 Packing /sleeve/bo500 MG100 rxns 25 mg1000 TESTS/0.65ml

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#33984278   2021/05/11 To Up

The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling.

Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in plants and animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane. The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11-dependent Ca influx, perturbation of subcellular structures, production of reactive oxygen species, and cell death. The results thus support that the ZAR1 resistosome acts as a calcium-permeable cation channel to trigger immunity and cell death.
Guozhi Bi, Min Su, Nan Li, Yu Liang, Song Dang, Jiachao Xu, Meijuan Hu, Jizong Wang, Minxia Zou, Yanan Deng, Qiyu Li, Shijia Huang, Jiejie Li, Jijie Chai, Kangmin He, Yu-Hang Chen, Jian-Min Zhou

1797 related Products with: The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling.

100ul100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#33984274   // To Up

Coevolution between bacterial CRISPR-Cas systems and their bacteriophages.

CRISPR-Cas systems provide bacteria and archaea with adaptive, heritable immunity against their viruses (bacteriophages and phages) and other parasitic genetic elements. CRISPR-Cas systems are highly diverse, and we are only beginning to understand their relative importance in phage defense. In this review, we will discuss when and why CRISPR-Cas immunity against phages evolves, and how this, in turn, selects for the evolution of immune evasion by phages. Finally, we will discuss our current understanding of if, and when, we observe coevolution between CRISPR-Cas systems and phages, and how this may be influenced by the mechanism of CRISPR-Cas immunity.
Bridget N J Watson, Jurre A Steens, Raymond H J Staals, Edze R Westra, Stineke van Houte

2225 related Products with: Coevolution between bacterial CRISPR-Cas systems and their bacteriophages.

25 units5 x 50 Pieces/case 5 mg200 assays100ul

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#33984272   // To Up

CRISPR transposons on the move.

CRISPR transposons (CASTs) represent unique mobile genetic elements that co-opted CRISPR-Cas immune systems for RNA-guided DNA transposition. However, CAST-encoded CRISPR arrays rarely match the CAST's chromosomal location. A recent publication in Cell helps resolve this paradox by revealing CRISPR-array-independent mechanisms of chromosomal homing unique to different CAST types.
Ioannis Mougiakos, Chase L Beisel

1000 related Products with: CRISPR transposons on the move.

200 units 2 ml 1 100 G1 6 ml Ready-to-use 500 Units10 μg

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