Search results for: 35kDa
#31546390 2019/08/13 To Up
On the formation of protein corona on colloidal nanoparticles stabilized by depletant polymers.To counter the undesired colloidal destabilization of nanoparticles in biologically-compatible media of high ionic strength (i.e. NaCl, phosphate buffer), polymers can be added to nanoparticle suspensions that will be used in biomedical applications. In these suspensions, polymers can promote high colloidal stability by manifestation of steric and/or depletion forces. However, little is known about the influence of these polymers on the interactions between nanoparticles and the biological components of the organism, such as proteins and cells. In this work, it was shown that the addition of the polymers (i) Pluronic-F127 (PF127), (ii) polyethylene glycol (PEG) of different molecular weights - 1.5, 12 and 35 kDa - and (iii) the protein bovine serum albumin (BSA) on colloidal silica nanoparticles (CSNPs; 135 nm) dispersed in phosphate-buffered saline (PBS) largely alter their colloidal stability through different mechanisms. Although all polymers were adsorbed on the CSNP surface, BSA maintained the CSNP dispersion in the medium by electrosteric stabilization mechanisms, while PEG and PF127 led to the occurrence of depletion forces between the particles. In addition, it was found that the interactions between polymers and CSNPs did not prevent proteins to access the nanoparticles' surface and have minimal effect on the formation of the protein corona when they were incubated in human blood plasma. On the other hand, BSA had a greater effect on the CSNP protein corona profile compared to other polymers (PEG and PF127). Together, these results confirm that biocompatible polymers PEG and PF127 can be used as colloidal stabilizing agents for nanoparticles since they preserve the accessibility of biomolecules to the nanoparticle surface, and they have little effect on the protein corona composition.
Romana Petry, Viviane M Saboia, Lidiane S Franqui, Camila de A Holanda, Thiago R R Garcia, Marcelo A de Farias, Antonio G de Souza Filho, Odair P Ferreira, Diego S T Martinez, Amauri J Paula
1797 related Products with: On the formation of protein corona on colloidal nanoparticles stabilized by depletant polymers.6 ml Ready-to-use 100ug100.00 ug200 25 ml 101 g 2 ml 100ul 25 ml Ready-to-use 100ug1 mg
#31525593 2019/09/07 To Up
Protein polymerization in dumpling wrappers influenced by folding patterns.The influences of folding patterns on the protein polymerization in dumpling wrappers were investigated. The dumpling dough sheet after the compounding rollers was folded with various patterns (control with no angle, 15°, 25°, 35° and 45° folding), before going through the sheeting and reduction rolls. Protein secondary structure, free sulfhydryl content, protein electrophoretic profiles, and texture of dumpling wrappers were determined. Results showed that folding could increase the proportion of α-helix conformation, and produce dumpling wrappers with enhanced toughness but reduce wrapper extensibility. The wrapper with 45° folding showed lower -SH content than the control and other folding angles. However, only a few variations in SDS band pattern and intensities were observed at the molecular weight position of around 35 kDa. Briefly, folding process could influence the gluten formation during the preparation of dumpling wrappers; the folding angle at 45° produced stronger gluten network and tougher wrappers.
Ting Liu, Meng Niu, Gary G Hou
2804 related Products with: Protein polymerization in dumpling wrappers influenced by folding patterns.1 Set1 Set1 Set1 Set1 mg1 Set1 Set1 Set1 Set100ug Lyophilized1 Set1 Set
#31499268 2019/09/06 To Up
Production, purification, and in vivo evaluation of a novel multiepitope peptide vaccine consisted of immunodominant epitopes of SYCP1 and ACRBP antigens as a prophylactic melanoma vaccine.Melanoma cells are significantly resistance to the current treatments. Therefore, the best option for high-risk populations is prevention. Recently, many preventive cancer vaccines have been developed. In our previous study, several bioinformatic tools were employed for selection of the most immunodominant epitopes of acrosin binding protein (ACRBP) and synaptonemal complex protein 1 (SYCP1) antigens to design multiepitope DNA and peptide cancer vaccines. In the current study, the final construct of the multiepitope DNA vaccine was placed into a pcDNA3.1 vector and then, subcloned into a pET-28a (+) expression vector for transfecting BL21 E. coli strain. The recombinant multiepitope peptide vaccine, weighing 6.35 kDa, was purified by Fast protein liquid chromatography technique (FPLC) and detected by western blotting. Subsequently, C57BL/6 mice were immunized by a mixture of the peptide vaccine and incomplete Freund's adjuvant (IFA) (four vaccinations with one-week intervals). Two weeks after the last vaccination, the serum levels of the peptide-specific IgG total, IgG2a, and IgG1 were measured by enzyme-linked immunosorbent assays (ELISA). Also, the immunized mice splenocytes efficacy for producing interleukin-4 (IL-4) and interferon-γ (IFN-γ) after stimulation with the peptide vaccine was evaluated. At last, the prophylactic effect of the peptide vaccine immunization was evaluated in B16-F10 murine melanoma model. The peptide vaccine immunization caused a significant increase in the serum levels of IgG1, IgG2a, and IgG2a. Also, the immunized mice splenocytes exhibited significantly higher ability to produce IL-4 (10-fold) and IFN-γ (16-fold) after stimulation with the peptide vaccine, in comparison with the PBS and IFA groups. The peptide immunized mice exhibited 50.2% and 43% decrease in the mean tumors' volume in comparison with PBS and IFA groups. Also, the mean survival time for the peptide immunized mice was 33 ± 1.3 days which was 5 and 6 days more than the PBS and IFA groups, respectively. The obtained results exhibit high efficacy of the designed multiepitope peptide vaccine for the immune system activation and anti-tumor prophylactic effects in the murine melanoma model.
Ashkan Safavi, Amirhosein Kefayat, Fattah Sotoodehnejadnematalahi, Mansoor Salehi, Mohammad Hossein Modarressi
2358 related Products with: Production, purification, and in vivo evaluation of a novel multiepitope peptide vaccine consisted of immunodominant epitopes of SYCP1 and ACRBP antigens as a prophylactic melanoma vaccine.48 samples96 assays24 tests100ug100 assays10 mg50 100ug25 mg
#31422160 2019/08/15 To Up
Purification and characterization of the carbonic anhydrase enzyme from horse mackerel (Trachurus trachurus) muscle and the impact of some metal ions and pesticides on enzyme activity.In this paper, the total carbonic anhydrase (CA) enzyme was purified from horse mackerel (Trachurus trachurus) muscle with a specific activity of 23,063.93 EU/mg, purification fold of 551.08, total activity of 1522.22 EU/mL and a yield of 18.50% using sulfanilamide affinity column chromatography. For obtaining the subunit molecular mass and enzyme purity, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for this part was performed and a single band was clearly recorded. The molecular mass of this enzyme was found approximately 35 kDa. The optimum temperature and pH values were obtained from Arrhenius plot. In addition, the inhibitory effects of different heavy metal ions (Fe, Cu, Co, Pb Hg and As) and some pesticides (thiram, clofentezine, propineb, deltamethrin, azoxystrobin and thiophanate) on horse mackerel (Trachurus trachurus) muscle tissue CA enzyme activities were investigated by utilizing esterase assay activity. The used metal ions and pesticides had IC values in the range of 0.21-13.84 mM and 3.78-70.58 mM, respectively.
Cuneyt Caglayan, Parham Taslimi, Cebrahil Türk, Fatih Mehmet Kandemir, Yeliz Demir, İlhami Gulcin
1662 related Products with: Purification and characterization of the carbonic anhydrase enzyme from horse mackerel (Trachurus trachurus) muscle and the impact of some metal ions and pesticides on enzyme activity.500 Units100 U1000 tests100.00 ul200ug100ug25 mg2.5 mg10 mg900 tests100ug
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#31174770 2019/05/23 To Up
Effects of Lactobacillus plantarum NJAU-01 on the protein oxidation of fermented sausage.This study was conducted to investigate the effects of Lactobacillus plantarum NJAU-01 isolated from Chinese dry-cured ham (Jinhua ham) on the regulation of protein oxidation of fermented sausages. Fermented sausages were prepared with different concentrations of L. plantarum NJAU-01 (10 CFU/g, 10 CFU/g and 10 CFU/g), and the commercial strain was used as positive control. The results showed that L. plantarum NJAU-01 at 10 CFU/g and 10 CFU/g significantly lowered the protein carbonyl content and protein surface hydrophobicity compared with the control (P < 0.05). The total sulfhydryl contents in L. plantarum NJAU-01 groups were significantly higher than that of the group without starter culture (P < 0.05). Significant changes were found in sarcoplasmic protein bands within 130-250 kDa, 100-130 kDa and 25-35 kDa during sausage fermentation (P < 0.05). Our data suggest that L. plantarum NJAU-01 has the potential to be an antioxidant starter culture in fermented sausages.
Qingfeng Ge, Sheng Chen, Rui Liu, Lei Chen, Bo Yang, Hai Yu, Mangang Wu, Wangang Zhang, Guanghong Zhou
1361 related Products with: Effects of Lactobacillus plantarum NJAU-01 on the protein oxidation of fermented sausage.5 G102 10 UG100 U1mg55201mg1mg1mg
#31158343 2019/05/31 To Up
Recombinant expression and characterization of major surface protein 4 from Anaplasma marginale.Anaplasma marginale is the rickettsia which causes the bovine anaplasmosis. The distribution of A. marginale is both tropical and subtropical regions of the world. The major surface protein 4 (MSP4) of this parasite was identified as an immunodominant protein. In this study, the full length of DNA encoding A. marginale MSP4 (AmMSP4) was cloned from the parasites. The open reading frame of msp4 coding sequence of Thailand strain is 849 bp. Phylogenetic analysis revealed that the msp4 coding sequence of A. marginale was highly conserved when compared with Anaplasma phagocytophilum. The recombinant plasmid was further transformed into the BL21-CodonPlus (DE3)-RIPL competent cells for over-expression of the recombinant major surface protein 4 of A. marginale (rAmMSP4). Sera from rabbit immunized with rAmMSP4 and from cattle infected with A. marginale were used to study the antigenicity of rAmMSP4 (35 kDa) and AmMSP4 (31 kDa). Both rAmMSP4 and AmMSP4 were recognized by these sera showing that recombinant and native AmMSP4 have conserved epitopes. Localization of Anaplasma parasites by immunofluorescence showed these parasites are distributed on both the membrane and the outside of infected erythrocytes. Regarding antigenicity, recombinant MSP4 could be used for immunodiagnostic purposes and as a possible vaccine candidate against anaplasmosis.
Amaya Watthanadirek, Runglawan Chawengkirttikul, Napassorn Poolsawat, Witchuta Junsiri, Dusit Boonmekam, Onrapak Reamtong, Panat Anuracpreeda
1970 related Products with: Recombinant expression and characterization of major surface protein 4 from Anaplasma marginale.100ul2x 100ug21001mg50050.00 ug51050.00 ug1010
#31097222 2019/05/13 To Up
A novel protein elicitor PeFOC1 from Fusarium oxysporum triggers defense response and systemic resistance in tobacco.In recent years, it is a hotspot research field on interaction mechanism between elicitor and plant. In this study, a novel hypersensitive response (HR)-inducing protein elicitor was isolated from the culture filtrate of Fusarium oxysporum f. sp. cubense and named PeFOC1, which consisted of 321 amino acids with a molecular weight of approximately 35 kDa. After the inducible expression in Escherichia coli and purification by ÄKTA explore system, the recombinant PeFOC1 also triggered a typical HR in tobacco. In addition, PeFOC1 induced a cascade of defense response in tobacco including production of hydrogen peroxide, deposition of callose, and accumulation of phenolic compounds. Moreover, PeFOC1 significantly improved systemic resistance of tobacco seedlings to tobacco mosaic virus and Pseudomonas syringae pv. tabaci. Real-time quantitative-PCR analysis indicated that several defense-related genes in tobacco, such as NtPR1a, NtNPR1, NtPAL, NtEDS1, NtPDF, and NtLOX, were all up-regulated by the treatment of PeFOC1. All these results collectively demonstrated that PeFOC1 triggered defense response and systemic acquired resistance (SAR) in tobacco. This research not only provides further research on immune mechanism between plant and elicitor, but also sheds new light on strategy for biocontrol in the future.
Songwei Li, Haizhen Nie, Dewen Qiu, Mingwang Shi, Qianhua Yuan
1726 related Products with: A novel protein elicitor PeFOC1 from Fusarium oxysporum triggers defense response and systemic resistance in tobacco.100μg1 Set1 Set1 Set1 Set100ug Lyophilized1 Set1 Set1 Set1 Set100ug Lyophilized100ug Lyophilized
#31071403 2019/05/06 To Up
Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed. Infrared spectrum and agarose-gel electrophoresis for extracted CS/DS were also investigated. The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa. Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively) and disulfated disaccharides in different percentages. The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64. Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS. Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time (TT) and prothrombin time (PT). In fact, CBG prolonged significantly (p < 0.05), aPTT and PT about 2.62 and 1.26 fold, respectively, greater than that of the negative control at a concentration of 1000 μg/mL. However, TT assay of CBG was prolonged 3.53 fold compared with the control at 100 μg/mL. The purified CS/DS displayed a promising anticoagulant potential, which may be used as a novel and soothing drug.
Hajer Bougatef, Fatma Krichen, Federica Capitani, Ikram Ben Amor, Jalel Gargouri, Francesca Maccari, Veronica Mantovani, Fabio Galeotti, Nicola Volpi, Ali Bougatef, Assaâd Sila
1833 related Products with: Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized0.1 ml100ug Lyophilized100ug Lyophilized100ug Lyophilized0.1 ml100ug Lyophilized100ug Lyophilized100ug Lyophilized
#31003915 2019/04/16 To Up
A peptide-based vaccine for Mycobacterium avium subspecies paratuberculosis.Recent efforts to develop a live attenuated vaccine against Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of Johne's disease (JD), revealed relA is important in Map virulence. Deletion of the relA gene impairs the ability of Map to establish a persistent infection. Analysis of the basis for this observation revealed infection with a relA deletion mutant (ΔrelA) elicits development of cytotoxic CD8 T cells (CTL) with the ability to kill intracellular bacteria. Further analysis of the recall response elicited by ΔrelA vaccination showed a 35 kDa membrane peptide (MMP) is one of the targets of the immune response, suggesting it might be possible to develop a peptide-based vaccine based on MMP. To explore this possibility, ex vivo vaccination studies were conducted with MMP alone and incorporated into a nanoparticle (NP) vector comprised of poly (D, L-lactide-co-glycolide) and monophosphoryl lipid A (PLGA/MPLA). As reported, ex vivo vaccination studies showed CD8 CTL were elicited with classic and monocyte derived dendritic cells (cDC and MoDC) pulsed with MMP alone and incorporated into a PGLA/MPLA vector. Incorporation of MMP into a NP vector enhanced the ability of CD8 CTL to kill intracellular bacteria. The findings indicate incorporation of MMP into a PGLA/MPLA nanoparticle vector is one of the possible ways to develop a MMP based vaccine for Johne's disease.
Gaber S Abdellrazeq, Mahmoud M Elnaggar, John P Bannantine, David A Schneider, Cleverson D Souza, Julianne Hwang, Asmaa H A Mahmoud, Victoria Hulubei, Lindsay M Fry, Kun-Taek Park, William C Davis
1134 related Products with: A peptide-based vaccine for Mycobacterium avium subspecies paratuberculosis.200 1 kit100ug Lyophilized100ug50 ug20 50 ug50 ug1 mg500 tests50 ug
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