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Search results for: 4-Bromo-2-methoxybenzoic acid CAS: [72135-36-5]

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#32942092   2020/08/28 To Up

Synthesis of polyacrylamide/polystyrene interpenetrating polymer networks and the effect of textural properties on adsorption performance of fermentation inhibitors from sugarcane bagasse hydrolysate.

Economical removal of fermentation inhibitors from lignocellulosic hydrolysate plays a considerable role in bioconversion of lignocellulose biomass. In this work, the textural properties of polyacrylamide/polystyrene interpenetrating polymer networks (PAM/PS IPNs) on adsorption of fermentation inhibitors from sugarcane bagasse hydrolysate (SCBH) were investigated for the first time. The results showed that, the specific surface area, pore diameter and surface polarity had important influence on its adsorption performance towards sugars, organic acids, furans and acid-soluble lignin. The PAM/PS IPNs under the optimal copolymerization situation achieved the high selectivity coefficients of 4.07, 14.9, 21.2 and 25.8 with respective to levulinic acid, furfural, hydroxymethylfurfural (HMF) and acid-soluble lignin, and had a low total sugar loss of 2.09%. Overall, this research puts forward a design and synthetic strategy for adsorbent to remove fermentation inhibitors from lignocellulosic hydrolysate.
Xue-Fang Chen, Li-Quan Zhang, Wen-Ping Xu, Can Wang, Hai-Long Li, Lian Xiong, Hai-Rong Zhang, Xin-de Chen

2086 related Products with: Synthesis of polyacrylamide/polystyrene interpenetrating polymer networks and the effect of textural properties on adsorption performance of fermentation inhibitors from sugarcane bagasse hydrolysate.

5 G100 U 8 ml 2500 Units1000 tests100ug0.2 mg250 U, 2.5 U/µl50 ug 100ug Lyophilized

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#32941607   2020/09/17 To Up

Detection of preQ0 deazaguanine modifications in bacteriophage CAjan DNA using Nanopore sequencing reveals same hypermodification at two distinct DNA motifs.

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Witold Kot, Nikoline S Olsen, Tue K Nielsen, Geoffrey Hutinet, Valérie de Crécy-Lagard, Liang Cui, Peter C Dedon, Alexander B Carstens, Sylvain Moineau, Manal A Swairjo, Lars H Hansen

1151 related Products with: Detection of preQ0 deazaguanine modifications in bacteriophage CAjan DNA using Nanopore sequencing reveals same hypermodification at two distinct DNA motifs.

1mg5010100ug100ug Lyophilized100ug1mg1g20100 100ug

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#32941125   2020/09/17 To Up

sp. nov. and sp. nov., isolated from deep-sea sediment of the South China Sea.

Two novel Gram-stain-positive bacteria, designated as SCSIO 52909 and SCSIO 52915, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea. Phenotypic, chemotaxonomic and genomic characteristics were investigated. These strains were aerobic and tested positive for catalase activity, oxidase activity and nitrate reduction. Optimal growth occurred at 28 °C, pH 7 and 3% salinity over 14 days cultivation. Its peptidoglycan structure was type A3α (l-Lys-l-Ala) and the only menaquinone was MK-8. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid. Their major fatty acids differed, but both contained iso-branched components of C 12-methyl. Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909 and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915. The two novel strains encoded genes for metabolism that are absent in most other species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase. Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized species, respectively. In the phylogenetic analysis, strains SCSIO 52909 and SCSIO 52915 were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus . Based on the data presented here, these two strains should be recognized as two new species in the genus , for which the names sp. nov., with the type strain SCSIO 52909 (=KCTC 49412=CGMCC 1.13853), and sp. nov., with the type strain SCSIO 52915 (=KCTC 49411=CGMCC 1.13852), are proposed.
Rou-Wen Chen, Cun Li, Yuan-Qiu He, Lin-Qing Cui, Li-Juan Long, Xin-Peng Tian

1283 related Products with: sp. nov. and sp. nov., isolated from deep-sea sediment of the South China Sea.

25 MG100ul 125 ml 25 mg11mg100 mg2250ul200ug

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#32937856   2020/09/14 To Up

MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in .

The Woronin body (WB) is a peroxisome-derived dense-core vesicle, a self-assembling hexagonal crystal of a single protein Hex1. This organelle is specific to the ascomycete fungi belonging to the Pezizomycotina subphylum by functioning in sealing septal pores in response to mycelium damage and the control of cell heterogeneity. We retrieved all available Hex1-domain containing proteins of different fungi from the GenBank database and found considerable length variations among 460 obtained Hex1 proteins. However, a highly conserved Hex1 domain containing 75 amino acid residues with a specific S/A-R/S-L consensus motif for targeting peroxisome is present at the carboxy-terminus of each protein. A homologous gene, named , was deleted in the entomopathogenic fungus It was found that MrHex1 was responsible for WB formation in and involved in sealing septal pores to maintain cell integrity and heterogeneity. Different assays indicated that, relative to the wild-type (WT) strain, ∆ demonstrated a growth defect on a solid medium and substantial reductions of conidiation, appressorium formation and topical infectivity against insect hosts. However, there was no obvious virulence difference between WT and mutants during injection of insects. We also found that ∆ could tolerate different stress conditions like the WT and the gene-rescued mutant of , which is in contrast to the reports of the stress-response defects of the null mutants of other fungal species. In addition to revealing the phenotypic/functional alterations of the deletion mutants between different pathotype fungi, the results of this study may benefit the understanding of the evolution and WB-control of fungal entomopathogenicity.
Guirong Tang, Yanfang Shang, Shiqing Li, Chengshu Wang

2967 related Products with: MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in .

0.1ml (1mg/ml)300 units100ug Lyophilized100ug Lyophilized100ug Lyophilized 100ul100ug Lyophilized100ug Lyophilized100ug Lyophilized25 µg100ug Lyophilized100ug Lyophilized

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#32937700   2020/05/25 To Up

Enhanced light-driven photothermocatalytic activity on selectively dissolved LaTiMnO perovskites by photoactivation.

LaTiMnO (x = 0.2 and 0.4) perovskite-type catalysts are synthesized by acrylamide polymerization route and etched with diluted HNO for oxidation of toluene as one of typical volatile organic compounds (VOCs). The substitution of Mn cations improves catalytic activity (toluene conversion increased from 0 to 50.6% by substituting 40% of Ti cations with Mn cations) by improving abilities of light absorption and light-to-heat conversion, and acid etching further promotes catalytic activity (toluene conversion increased from 50.6%-95.6% for LaTiMnO) by enlarging specific surface area, generating more surface active oxygen, strengthening mobility of surface oxygen, and improving low-temperature reducibility. To explore the origin of the light-driven photothermocatalytic activity of A-LaTiMnO by EPR analysis, O desorption and H consumption, we find that the light is not only as a thermal source to provide energy for toluene oxidation but also can promote oxidation reaction by photoactivation.
Enqi Yu, Jing Chen, Hongpeng Jia

2129 related Products with: Enhanced light-driven photothermocatalytic activity on selectively dissolved LaTiMnO perovskites by photoactivation.

100 assays1 ml1mg1 mg 96 Tests 100 assays400Tests100ug 25 ml Ready-to-use 500 ml

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#32937258   2020/09/13 To Up

Corrigendum to "Co-delivery of all-trans-retinoic acid and doxorubicin for cancer therapy with synergistic inhibition of cancer stem cells" [Biomaterials 37 (2015) 405-414].


Rong Sun, Yang Liu, Shi-Yong Li, Song Shen, Xiao-Jiao Du, Cong-Fei Xu, Zhi-Ting Cao, Yan Bao, Yan-Hua Zhu, Ya-Ping Li, Xian-Zhu Yang, Jun Wang

1613 related Products with: Corrigendum to "Co-delivery of all-trans-retinoic acid and doxorubicin for cancer therapy with synergistic inhibition of cancer stem cells" [Biomaterials 37 (2015) 405-414].

96T 1 G100ug 5 G

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#32936170   2020/09/16 To Up

A highly sensitive quartz crystal microbalance sensor modified with antifouling microgels for saliva glucose monitoring.

Saliva glucose detection based on quartz crystal microbalance (QCM) technology has become an important research direction of non-invasive blood glucose monitoring. However, the performance of this label-free glucose sensor is heavily deteriorated by the large amount of protein contaminants in saliva. Here, we successfully achieved the direct detection of saliva glucose by endowing the microgels on the QCM chip with superior protein-resistive and glucose-sensitive properties. Specifically, the microgel networks provide plenty of boric acid binding sites to amplify the signals of targeted glucose. The amino acid layer wrapped around the microgel and crosslinking layer can effectively eliminate the impact of non-specific proteins in saliva. The designed QCM sensor has a good linearity in the glucose concentration range of 0-40 mg L-1 in the pH range of 6.8-7.5, satisfying the physiological conditions of saliva glucose. Moreover, the sensor has excellent ability to tolerate proteins, enabling it to detect glucose in 50% human saliva. This result provides a new approach for non-invasive blood glucose monitoring based on QCM.
Qian Dou, Shiwen Wang, Zifeng Zhang, Yanxiang Wang, Zhipeng Zhao, Haijian Guo, Hongliang Liu, Qing Dai

2031 related Products with: A highly sensitive quartz crystal microbalance sensor modified with antifouling microgels for saliva glucose monitoring.

1,000 tests2 Pieces/Box96 tests50 assays4 Membranes/Box1 mg2 Pieces/Box 5KG200 mg

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#32935670   2020/09/16 To Up

PEGylated NALC-functionalized gold nanoparticles for colorimetric discrimination of chiral tyrosine.

In this work, acid and matrix-tolerant multifunctionalized gold nanoparticles (AuNPs) with an integrated chiral selector towards tyrosine (Tyr) and polyethylenglycol (PEG) chains were developed for visual chiral discrimination of Tyr in biological samples under acid conditions. In brief, AuNPs multifunctionalized with N-acetyl-l-cysteine (NALC) and PEG (PEG/NALC-AuNPs) were prepared via a simple strategy. In the presence of l-Tyr, the color of PEG/NALC-AuNP solution changed from red to gray, while no obvious color change was observed with the introduction of d-Tyr, which indicated that the introduction of PEG onto the surface of AuNPs has no effect on the chiral recognition between l-Tyr and NALC. A computer-aided molecular model was used to clarify the chiral recognition mechanism between NALC and Tyr enantiomers and to further guide the optimization of sensitivity. The resultant PEG/NALC-AuNP sensor presented a significantly improved stability under acid and alkali conditions compared with conventional NALC-AuNPs, resulting in a wider dynamic range (500 nM-100 μM) and a 50 times reduced detection limit by simply adjusting the pH of the sensor system under acid conditions (pH 2-2.5). More importantly, the PEG/NALC-AuNPs can realize the visual chiral discrimination of Tyr enantiomers in biological samples due to their significantly improved long-term stability and reduced interaction towards non-target species.
Xin-Yue Chen, Wei Ha, Xiao-Jie Jin, Yan-Ping Shi

2525 related Products with: PEGylated NALC-functionalized gold nanoparticles for colorimetric discrimination of chiral tyrosine.

100ug Lyophilized200 Cuvette10 mg100ug100ug100ug Lyophilized100tests 1000 ml 100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#32935229   2020/09/15 To Up

A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris.

Phytase is an additive in animal feed that degrades phytic acid in plant material, reducing feeding costs, and pollution from fecal phosphorus excretion. A multistrategy approach was adopted to improve the expression of E. coli phytase in Pichia pastoris. We determined that the most suitable signal peptide for phytase secretion was an α-factor secretion signal with an initial enzyme activity of 153.51 U/mL. Increasing the copy number of this gene to four increased phytase enzyme activity by 234.35%. PDI overexpression and Pep4 gene knockout increased extracellular phytase production by 35.33% and 26.64%, respectively. By combining favorable factors affecting phytase expression and secretion, the enzyme activity of the phytase-engineered strain was amplified 384.60% compared with that of the original strain. We also evaluated the potential for the industrial production of the engineered strain using a 50-L fed-batch fermenter and achieved a total activity of 30,246 U/mL after 180 h of fermentation.
Yuankun Helian, Yuanming Gai, Huan Fang, Yumei Sun, Dawei Zhang

2672 related Products with: A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris.

100 µg100 10 300 units10ìg

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#32934149   2020/09/15 To Up

Origins and evolution of cuticle biosynthetic machinery in land plants.

The aerial epidermis of land plants is covered with a hydrophobic cuticle that protects the plant against environmental stresses. Although the mechanisms of cuticle biosynthesis have been extensively studied in model plants, particularly in seed plants, the origins and evolution of cuticle biosynthesis are not well understood. In this study, we performed a comparative genomic analysis of core components that mediate cuticle biosynthesis, and characterized the chemical compositions and physiological parameters of cuticles from a broad set of embryophytes. Phylogenomic analysis revealed that the cuticle biosynthetic machinery originated in the last common ancestor of embryophytes. Co-expansion and coordinated expression are evident in core genes involved in the biosynthesis of two major cuticle components: the polymer cutin and cuticular waxes. Multi-species analyses of cuticle chemistry and physiology further revealed higher loads of both cutin and cuticular waxes in seed plants than in bryophytes, as well as greater proportions of di- and trihydroxy acids, dicarboxylic acids, very-long-chain alkanes, and >C28 lipophilic compounds. This can be associated with land colonization and the formation of cuticles with enhanced hydrophobicity and moisture retention capacity. These findings provide insights into the evolution of plant cuticle biosynthetic mechanisms.
Lingyao Kong, Yanna Liu, Pengfei Zhi, Xiaoyu Wang, Bo Xu, Zhizhong Gong, Cheng Chang

2653 related Products with: Origins and evolution of cuticle biosynthetic machinery in land plants.

100 μg100 assays100 μg100ug Lyophilized100ug96T 25 G100 μg12

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