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#33202065   2020/11/17 To Up

Residual red cells in blood components: A multisite study of fully automated enumeration using a hematology analyzer.

Manufacture of platelet concentrates (PCs) and plasma may fail to remove all residual red blood cells (rRBCs). Measuring rRBCs for compliance to guidelines has proven challenging, leading to an absence of a consensus methodology. Sysmex hematology analyzers with the Blood Bank mode (BB mode) analysis option offer the potential for automated rRBC counting. We therefore performed a two-site appraisal of the system.
Chloe Cavagnetto, Richard Alejo Blanco, Hollie McKenna, Laura Willmott, Elif Aydogdu, Nicola Akinyemi, Helena Standring, Simon Procter, Johan W Lagerberg, Elin Johansson, Harry Croxon, Dirk de Korte, Stephen F Garner, Atsushi Shirakami, Jarob Saker, Joachim Linssen, Rebecca Cardigan

1116 related Products with: Residual red cells in blood components: A multisite study of fully automated enumeration using a hematology analyzer.

111.00 flask96 tests2 ml50 ul100 µg50 mg50 ul11

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#33170953   2020/11/10 To Up

Quantitative positron emission tomography imaging in the presence of iodinated contrast media using electron density quantifications from dual-energy computed tomography.

As preparation for future PET/DECT imaging modality and new possible clinical applications, the study aimed to evaluate the utility of clinically available spectral results from a dual-energy CT (DECT) system for improving attenuation corrections of PET acquisitions in the presence of iodinated contrast media. The dependence of the accuracy of PET quantification values, reconstructed with conventional and spectral-based attenuation corrections, was examined as a function of the amount of iodine content and x-ray radiation exposure.
Nadav Shapira, Joshua Scheuermann, Amy E Perkins, Johoon Kim, Leening P Liu, Joel S Karp, Peter B Noël

2876 related Products with: Quantitative positron emission tomography imaging in the presence of iodinated contrast media using electron density quantifications from dual-energy computed tomography.

500 ml16 Arrays/Slide1 mg96 tests

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#33142386   // To Up

[Determination of N-[4- (4-aminobenzyl) -phenyl] acetamide hemoglobin adduct in blood by ultra performance liquid chromatography-tandem mass spectrometry].

To establish the method for the determination of N-Acetyl-4, 4'-diaminodiphenylmethane (AcMDA) adduct in the hemoglobin by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) . The 20 mg hemoglobin sample was weighed into a 15 ml centrifuge tube, adding 20 μg/L internal standard solution AcMDA-D8 10 μl, then hydrolyzed with 1 ml 0.1 mol/L sodium hydroxide solution at 37°C for 0.5 hours, extracted with dichloromethane and concentrated by vacuum concentrator. The residue was dissolved in acetonitrile and detected by UPLC-MS/MS, then quantitative by internal standard method. The linearity of the method was good at the range of 0.05-25.00 μg/L with a correlation coefficient of 0.9996, the detection limit (LOD) and limit of quantitation (LOQ) were 0.07 and 0.2 ng/g Hb, respectively. The recovery rate was ranged from 91.0%-95.4%; the relative standard deviation () of intra-and inter-batch precision were 4.5%-6.3% and 3.7%-4.4%, respectively. The determination method meet the requirement of GBZ/T 210.5-2008.
X K Wang, H B Li, H F Yan

1766 related Products with: [Determination of N-[4- (4-aminobenzyl) -phenyl] acetamide hemoglobin adduct in blood by ultra performance liquid chromatography-tandem mass spectrometry].

20 1 G400Tests100 20 20 20 1 mg150/kit 100 G20 100

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#32835940   2020/07/10 To Up

Inflammatory cell profile using different autologous fibrin protocols.

Autologous fibrin has been widely used in surgical procedures for both soft and hard tissue repair. There are different protocols and devices to obtain this matrix, with varying centrifugal time, gravity force, speed, angle of the sample tube and spinning radius. The aim of this study was to compare three methods of obtaining autologous fibrin: L-PRF using the Intra-Spin L-PRF centrifuge (Dohan protocol), the advanced PRF (A-PRF) using the Intra-Spin L-PRF centrifuge and autologous leukocyte fibrin (ALF), using the Kasvi centrifuge. Venous blood was collected from 7 healthy volunteers, which were submitted to the 3 different methods of centrifugation. The membranes were tissue-processed and evaluated by immunohistochemistry for CD3, CD20, CD68 and CD138. For CD68+, a lower number of cells was immunolabelled in the L-PRF group when compared to the other groups (A-PRF and ALF). For CD3+, a lower number of immunolabellated cells was observed in the ALF group when compared to the remaining groups (p < 0.05). In the A-PRF group, the CD20+ cell count was lower than in the remaining groups. No difference was observed in CD138+ cell counts between the groups. The 3 protocols tested are suitable for obtaining autologous fibrin membranes.
Ledson Sampaio Nogueira, Elizabeth Ferreira Martinez, Daiane Cristina Peruzzo, Júlio César Joly, Marcelo Henrique Napimoga

2009 related Products with: Inflammatory cell profile using different autologous fibrin protocols.

1 mg24 wells10 ug24 wells3x 500 ml5 x 50 ug100 μg50 ug10030ml10 lt

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#32316446   2020/04/17 To Up

Ultrasound Standing Wave-Based Cell-to-liquid Separation for Measuring Viscosity and Aggregation of Blood Sample.

When quantifying mechanical properties of blood samples flowing in closed fluidic circuits, blood samples are collected at specific intervals. Centrifugal separation is considered as a required procedure for preparing blood samples. However, the use of centrifuge is associated with several issues, including the potential for red blood cell (RBC) lysis, clotting activation, and RBC adhesions in the tube. In this study, an ultrasonic transducer is employed to separate RBCs or diluent from blood sample. The ultrasonic radiation force is much smaller than the centrifugal force acting in centrifuge, it can avoid critical issues occurring under centrifuge. Then, the RBC aggregation and blood viscosity of the blood sample are obtained using the microfluidic technique. According to the numerical results, ultrasonic transducers exhibited a maximum quality factor at an excitation frequency of 2.1 MHz. Periodic pattern of acoustic pressure fields were visualized experimentally as a column mode. The half wavelength obtained was as 0.5 λ = 0.378 ± 0.07 mm. The experimental results agreed with the analytical estimation sufficiently. An acoustic power of 2 W was selected carefully for separating RBCs or diluent from various blood samples (i.e., = 20% ~ 50%; diluent: plasma, 1x phosphate-buffered saline (PBS), and dextran solution). The present method was employed to separate fixed blood samples which tended to stack inside the tube while using the centrifuge. Fixed RBCs were collected easily with an ultrasonic transducer. After various fixed blood samples with different base solutions (i.e., glutaraldehyde solution, 1x PBS, and dextran solution) were prepared using the present method, RBC aggregation and the viscosity of the blood sample are successfully obtained. In the near future, the present method will be integrated into ex vivo or in vitro fluidic circuit for measuring multiple mechanical properties of blood samples for a certain longer period.
Gwangho Kim, Sanghwa Jeong, Yang Jun Kang

1147 related Products with: Ultrasound Standing Wave-Based Cell-to-liquid Separation for Measuring Viscosity and Aggregation of Blood Sample.

500 gm.One 96-Well Microplate Ki1 kit(96 Wells)One 96-Well Microplate Ki25 mgOne 96-Well Microplate Ki50 samplesOne 96-Well Microplate Ki4 X 250 ml.1,000 tests

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#32231163   2020/03/28 To Up

Hypergravity Activates a Pro-Angiogenic Homeostatic Response by Human Capillary Endothelial Cells.

Capillary endothelial cells are responsible for homeostatic responses to organismic and environmental stimulations. When malfunctioning, they may cause disease. Exposure to microgravity is known to have negative effects on astronauts' physiology, the endothelium being a particularly sensitive organ. Microgravity-related dysfunctions are striking similar to the consequences of sedentary life, bed rest, and ageing on Earth. Among different countermeasures implemented to minimize the effects of microgravity, a promising one is artificial gravity. We examined the effects of hypergravity on human microvascular endothelial cells of dermal capillary origin (HMEC-1) treated at 4 for 15 min, and at 20 for 15 min, 3 and 6 h. We evaluated cell morphology, gene expression and 2D motility and function. We found a profound rearrangement of the cytoskeleton network, dose-dependent increase of Focal Adhesion kinase (FAK) phosphorylation and Yes-associated protein 1 (YAP1) expression, suggesting cell stiffening and increased proneness to motility. Transcriptome analysis showed expression changes of genes associated with cardiovascular homeostasis, nitric oxide production, angiogenesis, and inflammation. Hypergravity-treated cells also showed significantly improved motility and function (2D migration and tube formation). These results, expanding our knowledge about the homeostatic response of capillary endothelial cells, show that adaptation to hypergravity has opposite effect compared to microgravity on the same cell type.
Chiara De Cesari, Ivana Barravecchia, Olga V Pyankova, Matteo Vezza, Marco M Germani, Francesca Scebba, Jack J W A van Loon, Debora Angeloni

1804 related Products with: Hypergravity Activates a Pro-Angiogenic Homeostatic Response by Human Capillary Endothelial Cells.

1.00 flask0.1 mg1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask0.5 ml1.00 flask0.5 ml16 Arrays/Slide1mg

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#32201409   2020/03/20 To Up

Organic Ion-associate Phase Extraction/Back-microextraction for the Preconcentration and Determination of Lithium Using 2,2,6,6-Tetramethyl-3,5-heptanedione by Liquid Electrode Plasma Atomic Emission Spectrometry and GF-AAS in Environmental Water.

We developed an ion-associate phase (IAP)-extraction/acid back-extraction system for the preconcentration and atomic spectrometric determination of lithium trace amounts in water. The chelating reagent for lithium also works as a constituent of the extraction phase. The lithium in a 10 mL sample solution was converted through a chelate complex reaction with 2,2,6,6-tetramethyl-3,5-heptanedione (HDPM). The addition of a benzyldimethyltetradecylammonium ion caused the formation of IAP suspension in the solution. Centrifugation of the solution led to the isolation of a liquid organic phase and the lithium complex was extracted as the upper phase from the centrifuge tube. After the aqueous phase was removed, lithium was back-extracted with a 400 μL nitric acid solution from the IAP. The acid phase was measured using liquid-electrode-plasma atomic-emission-spectrometry (LEP-AES) or graphite-furnace atomic-absorption spectroscopy (GF-AAS). The detection limits were 0.02 mg/L for LEP-AES and 0.02 μg/L for GF-AAS. This system was applied to the determination of environmental water. The HDPM in the organic phase was reusable.
Kenta Mizuna, Ryo Murashima, Takuya Okazaki, Kazuto Sazawa, Hideki Kuramitz, Shigeru Taguchi, Keiko Nakayama, Tamotsu Yamamoto, Yuzuru Takamura, Noriko Hata

2432 related Products with: Organic Ion-associate Phase Extraction/Back-microextraction for the Preconcentration and Determination of Lithium Using 2,2,6,6-Tetramethyl-3,5-heptanedione by Liquid Electrode Plasma Atomic Emission Spectrometry and GF-AAS in Environmental Water.

96T100ug20 0.05 mg200ug1 mg1 g1000 tests2.5 mg

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#31980262   2020/01/13 To Up

Air-assisted liquid-liquid microextraction based on solidification of floating deep eutectic solvent for the analysis of ultraviolet filters in water samples by high performance liquid chromatography with the aid of response surface methodology.

For this work, a novel air-assisted liquid-liquid microextraction based on solidification of floating deep eutectic solvent (AA-LLME-SFDES), coupled with a high performance liquid chromatography (HPLC) method was developed for the detection of benzophenone and salicylate ultraviolet filters in water samples. Three types of fatty acid-based hydrophobic deep eutectic solvents (DESs) with low viscosity, low-density, and melting point close to room temperature were prepared and employed as extraction solvents. This air-assisted liquid-liquid microextraction was carried out in a glass centrifuge tube. Subsequently, the glass tube was introduced into ice-water bath and held for 3 min, during which the upper DES phase was solidified. The water phase was easily extracted using a syringe equipped with a long needle, and later, the glass tube was removed from ice-water bath. The solidified DES phase was immediately melted at room temperature and used for HPLC analysis. The response surface methodology was employed to optimize some influencing parameters such as the volume of the extraction solvent, the pH value of sample solution, the number of extraction cycles, and the addition of salt. A quadratic model, namely a central composite design, was used to replace the conventional single factor analysis. It was found that under optimal conditions, the limits of determination and quantification were 0.045-0.54 µg L and 0.15-2.0 µg L, respectively. The relative standard deviations for inter-day (n = 5) and intra-day (n = 5) precision were ≤ 4.2%, whereas the enrichment factors for the ultraviolet filters were obtained from 41 to 50. Furthermore, this novel method was successfully employed for the detection of benzophenone and salicylate ultraviolet filters from real water samples. The recoveries ranged from 87.5% to 105.8%, whereas the RSDs were lower than 3.6%.
Kaige Zhang, Shuangying Li, Yunhe Wang, Jing Fan, Guifen Zhu

2498 related Products with: Air-assisted liquid-liquid microextraction based on solidification of floating deep eutectic solvent for the analysis of ultraviolet filters in water samples by high performance liquid chromatography with the aid of response surface methodology.

25 mg100 100 20 100 1 g20 20 20 20 20 100 mg

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