Only in Titles

Search results for: CENTRIFUGE TUBE HS,30ML

paperclip

#34289718   2021/07/22 To Up

Pre-analytical sample handling effects on blood cytokine levels: quality control of a COVID-19 biobank.

We investigated the effect of pre-analytical sample handling variations on coronavirus disease 2019-relevant circulating cytokine levels IFN-γ, IL-10, IL-12p70, IL-17A, IL-6 and TNF-α. We collected blood in different collection tubes (ethylenediaminetetraacetic acid, sodium citrate, lithium heparin, serum), and subjected ethylenediaminetetraacetic acid plasma to among others increasing delays in centrifugation or -80°C-storage. Six subjects were included in each experimental condition. Cytokine levels were measured in these samples using the Simoa Cytokine 6-plex kit. Different tube types resulted in different blood cytokine levels. IL-17A and IL-6 levels declined with 3 h centrifugation delay. IFN-γ levels declined with 24 h postcentrifugation storage delay. IL-17A levels declined with 2-week storage delay. It is recommended to centrifuge tubes quickly following collection, for accurate cytokine measurement.
Inge Mw Verberk, Esther J Nossent, Hetty J Bontkes, Charlotte E Teunissen

1956 related Products with: Pre-analytical sample handling effects on blood cytokine levels: quality control of a COVID-19 biobank.

32-50 Sample Kit4 Sample Kit1-8 Sample Kit16 Arrays/Slide4 Sample Kit1-8 Sample Kit2 Sample Kit8 Sample Kit16-22 Sample Kit4 Sample Kit32-50 Sample Kit16 Arrays/Slide

Related Pathways

paperclip

#34283582   2021/07/20 To Up

A Novel Pretreatment Device Integrating Magnetic-Assisted Dispersive Extraction and Ultrasonic Spray Separation for Speciation Analysis of Arsenic in Whole Blood by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry.

Speciation analysis of arsenic in blood is essential for identifying and quantifying the exposure of arsenic and studying the metabolism and toxicity of arsenic. Herein, a novel pretreatment device is rationally designed and used for speciation analysis of arsenic in whole blood by ion chromatography-inductively coupled plasma-mass spectrometry (IC-ICP-MS). The sample centrifuge tubes containing blood, reagents, and a magnetic stir bar are placed on the fidget spinner of the pretreatment device. When flicking the fidget spinner rotation with the finger, the magnetic stir bar in the tube rotates in three dimensions under the magnetic field, thereby assisting dispersive extraction of arsenic species by the mixing of blood with reagents. Afterward, the arsenic extract is separated in situ from the blood matrix using an ultrasonic spray sheet covered with a filter and ultrafiltration membrane, which is directly used for subsequent IC-ICP-MS analysis. For 100 μL of blood, the whole pretreatment operation can be completed within 10 min. With As(III), As(V), MMA, and DMA in blood as analytes, the use of the present pretreatment device will hardly lead to the loss and transformation of arsenic species, and the extraction efficiency of the total arsenic is more than 96%. When the pretreatment device is coupled to IC-ICP-MS, the detection limits of four arsenic species in whole blood are 0.017-0.023 μg L, and precisions are within 2.3-4.2%. This pretreatment device provides a simple, fast, efficient, and low-cost tool for extraction and separation of arsenic species in whole blood, opening a new idea for the pretreatment of complex samples.
Xiao Zhang, Shuang Liu, Xing Wei, Yong-Liang Yu, Jian-Hua Wang

1969 related Products with: A Novel Pretreatment Device Integrating Magnetic-Assisted Dispersive Extraction and Ultrasonic Spray Separation for Speciation Analysis of Arsenic in Whole Blood by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry.

case500 gm.1 L.100 extractions100 extractions96T 100 UG96T5 mgcase

Related Pathways

paperclip

#34279775   2021/07/19 To Up

Single-Nucleotide Polymorphism Associates' β-Tubulin Isotype-1 Gene in Onchocerca volvulus Populations in Ivermectin-Treated Communities in Taraba State, Nigeria.

The occurrence of Single-Nucleotide Polymorphisms (SNPs) associated with repeated ivermectin treatment and sub-optimal responses reported by previous findings is of great concern in Onchocerciasis endemic areas. This study investigated SNPs' occurrence after 15 years of ivermectin intervention in Onchocerciasis endemic communities in two Local Government Areas of Taraba State, Nigeria.
Danlami E Akafyi, Iliya S Ndams, Ishaya H Nock, Gloria Chechet, Alfons Renz

1405 related Products with: Single-Nucleotide Polymorphism Associates' β-Tubulin Isotype-1 Gene in Onchocerca volvulus Populations in Ivermectin-Treated Communities in Taraba State, Nigeria.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

Related Pathways

paperclip

Error loading info... Pleas try again later.
paperclip

#34248097   2021/07/09 To Up

Enzyme-assisted Solvent Extraction of High-yield Paeonia suffruticosa Andr. Seed Oil and Fatty Acid Composition and Anti-Alzheimer's Disease Activity.

Enzyme-assisted solvent extraction (EASE) of Paeonia suffruticosa Andr. seed oil (PSO) was optimized by response surface methodology (RSM). The fatty acid composition and anti-Alzheimer's disease (AD) activity of PSO were analyzed. An enzyme mixture composed of cellulase and hemicellulase (1:1, w/w) was most effective in determining the extraction yield of PSO. The ideal extraction conditions were a pH value of 5.1, an enzymolysis time of 68 min, and a temperature of 50°C. The average extraction yield of PSO was 38.2 mL/100 g, 37.4% higher than that of untreated peony seed (27.8 mL/100 g). The fatty acid composition of PSO under optimal conditions for EASE was analyzed by gas chromatography-mass spectrometry (GC-MS). The predominant unsaturated fatty acids of PSO were determined to be more than 90.00%, including n-3 α-linolenic acid (43.33%), n-6 linoleic acid (23.40%) and oleic acid (23.59%). In this experiment, the anti-AD effect of PSO was also analyzed by performing learning and memory ability tests with Drosophila. PSO retarded the decrease in climbing ability in AD Drosophila. The 1% and 5% PSO groups were significantly different from the model group (p < 0.05). The smell short-term memory ability test revealed the number of Drosophila in barrier and barrier-free centrifuge tubes in each group. PSO feeding improved learning and memory in AD Drosophila, with the highest number entering the barrier-free centrifuge tube. The performance index (PI) measured by the Pavlov olfactory avoidance conditioning test also demonstrated the effect of PSO on the learning and memory abilities of Drosophila. The PI of the PSO group was significantly increased compared to that of the model group. HE-stained brain tissue sections of AD Drosophila showed higher neurodegenerative changes, while PSO significantly reduced neurodegenerative damage. These results indicated that PSO can significantly improve the cognitive function of AD Drosophila and may help to prevent AD.
Gang Wei, Zidong Zhang, Dongmei Fu, Yuanyuan Zhang, Weipeng Zhang, Yuangang Zu, Lin Zhang, Zhi Zhang

1836 related Products with: Enzyme-assisted Solvent Extraction of High-yield Paeonia suffruticosa Andr. Seed Oil and Fatty Acid Composition and Anti-Alzheimer's Disease Activity.

50 ug 100ug100ug200 100ug Lyophilized100ug Lyophilized500 tests1,000 tests500g

Related Pathways

paperclip

#34227373   // To Up

[Determination of hydrazine in prednisolone by derivatization-gas chromatography-triple quadrupole mass spectrometry].

Prednisolone is an adrenal glucocorticoid drug with immunosuppressive, anti-inflammatory, anti-allergic, and antiviral effects that are widely exploited in clinical treatment. The hydrazine residue to prednisolone directly affects medication safety and threatens the patient's health. At present, there are no relevant laws, regulations, and standards to control the residual limit of hydrazine in drugs at home or abroad. Therefore, a simple, rapid, accurate, reliable, sensitive, and selective method is urgently needed for the determination of trace hydrazine in prednisolone. Hydrazine has strong polarity and reductivity, with unstable physical and chemical properties, thus being easily oxidized. In addition, because of the lack of chromophores and low molecular weight, the detection of hydrazine is very difficult. Therefore, a derivative reagent should be introduced to reduce its polarity and generate a derivative product with a high molecular weight as well as stable physical and chemical properties. Acetone, as a common laboratory reagent, is inexpensive and can rapidly react with hydrazine; therefore, it is an ideal derivative reagent for the determination of hydrazine. In this study, a method based on precolumn derivatization with gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) was developed for the determination of hydrazine in prednisolone by optimizing the derivatization reagent, GC and MS conditions, solvent system, and derivatization conditions. Method validation was then carried out using the established method, and the results were satisfactory. In this study, 1 g of prednisolone sample was weighed and placed in a 10 mL centrifuge tube with a plug; then, a methanol-dichloromethane dilution solvent (14∶23, v/v) was added to the scale line, and the sample was vortexed until completely dissolved. About 100 μL of the test solution prepared above was pipetted into the sample vial, followed by the addition of 900 μL acetone. The resulting solution was vortexed and mixed well. The sample was diluted and derivatized simultaneously in acetone solution, acetone/methanol-dichloromethane dilution solvent (9∶1, v/v), and then detected and analyzed by GC-MS/MS. In this study, the derivatization reaction between hydrazine and acetone did not require the addition of acetic acid and ultrasound conditions, or the use of other reagents for the extraction operation. The reaction was instantaneous, and rapid determination of hydrazine in prednisolone could be achieved. The standard curve was obtained with a good correlation coefficient (=0.9999) in the range 1-12 μg/L. The limits of detection and quantitation were 0.03 mg/kg and 0.10 mg/kg, respectively. The relative standard deviation (RSD) of injection precision was 1.10%. The recoveries and repeatability were good; the recoveries of low-, medium-, and high-concentration spiked samples were 96.15%-96.46% at spiked concentrations of 1, 6, and 12 μg/L, respectively, and the corresponding RSDs were 1.77%-2.12%. The intermediate precision was good, and the RSD of the determination results obtained on the same instrument by different laboratory technicians at different times was 1.77%. The durability was good, and the degree of influence of the detection results was studied by changing the chromatographic conditions. Under the original condition or conditions with initial column temperature ±5 ℃, heating rate ±2 ℃/min, or column flow rate ±0.1 mL/min, the hydrazine content in the sample solution at a spiked concentration of 6 μg/L was detected, and the RSD of the detection results was 2.58%. The established method was applied to detect hydrazine in a prednisolone standard substance procured from the market and nine batches of prednisolone samples provided by a pharmaceutical company. No hydrazine was detected in any of these samples. The established method is simple, reliable, highly sensitive, and highly selective, and it can be applied for the detection of hydrazine in prednisolone.
Chong Qian, Mei Zhang, Shanshan Liu, Xinlei Gou, Wei Wang, Guanghui Hu

2511 related Products with: [Determination of hydrazine in prednisolone by derivatization-gas chromatography-triple quadrupole mass spectrometry].

250 TESTS400Tests100 μg1 mg100 μg

Related Pathways

paperclip

#34227363   // To Up

[Determination of nine -nitrosamines in animal derived foods by QuEChERS-isotope dilution combined with gas chromatography-tandem mass spectrometry].

In this study, a comprehensive analytical method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the determination of nine -nitrosamines in animal derived foods. There are many kinds of -nitrosamines in foods that are harmful to human health. However, the national standard GB 5009.26-2016 pertains only to the detection of -dimethylnitrosamine; there are many drawbacks of this method, such as complicated sample preparation, low recovery rate, and poor reproducibility. Hence, it is of practical significance to establish a method for the simultaneous determination of a variety of -nitrosamines. The optimal extraction conditions for the developed method were as follows: 10.0 g aliquots of the sample were placed in a 50 mL centrifuge tube, followed by the addition of 10 mL acetonitrile and 200 μL internal working standard solutions. After 30 min of freezing treatment, 4 g magnesium sulfate and 1 g sodium chloride were added for dehydration, and the tube was centrifuged at 9000 r/min for 5 min. After vortex centrifugation, 5 mL of the clear supernatant was purified using 150 mg polystyrene divinylbenzene (PLS-A). The purified extracts were dewatered using 1.6 g MgSO and 0.4 g NaCl, and then filtered through a 0.22 μm membrane filter unit prior to GC-MS/MS analysis. Temperature-programmed was applied at an initial temperature of 50 ℃. After 0.16 min, the temperature was raised to 220 ℃ at the rate of 900 ℃/min for 5 min. -Nitrosamines were separated on an HP-Innowax column (30 m×0.25 mm×0.25 μm). Identification and quantification were achieved using an electron impact ion (EI) source in positive ion mode with multiple reaction monitoring (MRM). The internal standard method was used to quantify the -nitrosamines. Under the optimal conditions, the correlation coefficients of the standard calibration curves were not less than 0.99 in the range of 0.1-50.0 μg/L. The limits of detection were 0.03-0.30 μg/kg (=3), and limits of quantification were 0.15-1.00 μg/kg (=10). At spiked levels of 0.5, 1.0, and 3.0 μg/kg, the average recoveries of -nitrosamines in spiked samples ranged from 80.4% to 98.5%, with relative standard deviations between 2.41% and 12.50%. This method was used to determine animal derived food products, except -itrosomethylethylamine and -nitrosomorpholine, others were founded. The results showed that -nitrosamines levels in salted aquatic products were generally higher than those of the other samples. The method established in this study is simple to operate, and it does not require any time-consuming distillation extraction. Furthermore, there is minimal consumption of samples and reagents; consequently, the experiment cost is reduced, and the method is environmentally friendly. This method has theoretical and practical significance for the control of -nitrosamines residues in animal derived foods, establishment of detection standards, and corresponding management measures.
Xiangyi Kong, Lili Zhuang, Enhua Fang, Peng Lin, Zilong Zheng, Xianghua Zheng, Dunming Xu

1386 related Products with: [Determination of nine -nitrosamines in animal derived foods by QuEChERS-isotope dilution combined with gas chromatography-tandem mass spectrometry].

400Tests25mg900 tests100 units250 TESTS

Related Pathways

paperclip

#34199408   2021/06/02 To Up

Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization.

Blood sample collection and rapid separation-critical preanalytical steps in clinical chemistry-can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.
Kyungjin Hong, Gabriella Iacovetti, Ali Rahimian, Sean Hong, Jon Epperson, Clara Neal, Tifany Pan, Angela Le, Eric Kendall, Kory Melton, Greg J Sommer, Ulrich Y Schaff

2453 related Products with: Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization.

96TUnit

Related Pathways

paperclip

Error loading info... Pleas try again later.
paperclip

#34133570   2021/06/11 To Up

Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes).

In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.
H Kahraman, C C Karaderi

1430 related Products with: Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes).

5 sets0.1mg5 ml1 set 1 ml1 set (5 x 1 ml)1 set100μg1 ml 100ul1 ml

Related Pathways