Search results for: 6GLL
#35026959 
2022/01/14
To Up
At least four distinct blue cationic phthalocyanine dyes sold as "alcian blue" raises the question: what is alcian blue?
We investigated physicochemical characteristics of dye lots sold as "alcian blue" using the Biological Stain Commission (BSC) precipitation test, differential scanning calorimetry, high performance liquid chromatography, thin layer chromatography and UV/visible spectroscopy. Four blue phthalocyanine dyes were detected in 11 commercial dye lots. These four included the original ingrain blue 1 CI 74000 dye and the dye sold with the name "alcian blue pyridine variant"; we discuss also the possible identity of the additional two dyes. A proposed extension to the BSC analytic scheme is presented that could distinguish three categories of commercial alcian blue dyes from each other and from the original alcian blue 8G.S Ali, R W Dapson, R W Horobin, J A Kiernan, A Kazlauciunas
2823 related Products with: At least four distinct blue cationic phthalocyanine dyes sold as "alcian blue" raises the question: what is alcian blue?
1 kit(s) 500 ml 250 ml 1000 ml 1 kit(s) 100 Slides 500 ml 10G 1 kit(s) 250 ml 100 Slides 1000 ml
Related Pathways
-
No related Items
#6157821 //
To Up
The molecular biology of histochemical staining by cationic phthalocyanin dyes: the design of replacements for Alcian Blue.
Four cationic copper phthalocyanin dyes are compared with Alcian Blue 8G for specificity of interaction with a range of natural and synthetic polyanions. The CEC system is used to estimate dye-substrate affinities. The results support the hypothesis that the tissue staining pattern of Alcian Blue is determined by (a) exclusion of the dye from polynucleotide stacked base pairs by steric hindrance due to bulky substituents on the chromophore and (b) the presence of pendant quaternary charges whose affinity for negative sites on polyanionic substrates is without regard for the precise chemical nature of the anion. These conclusions, and practical considerations lead to the choice of quaternized Astra Blue 6GLL as a replacement for Alcian Blue (now no longer manufactured). It is superior to Alcian Blue in pH range, and stability in solution. It is easily prepared from a readily available precursor.J E Scott