Gentaur Pub

#38647893   2022/07/30 To Up

Production of free fatty acids from various carbon sources by Ogataea polymorpha.

Energy shortage and environmental concern urgently require establishing the feasible bio-refinery process from various feedstocks. The methylotrophic yeast Ogataea polymorpha is thermo-tolerant and can utilize various carbon sources, such as glucose, xylose and methanol, which makes it a promising host for bio-manufacturing. Here, we explored the capacity of O. polymorpha for overproduction of free fatty acids (FFAs) from multiple substrates. The engineered yeast produced 674 mg/L FFA from 20 g/L glucose in shake flask and could sequentially utilize the mixture of glucose and xylose. However, the FFA producing strain failed to survive in sole methanol and supplementing co-substrate xylose promoted methanol metabolism. A synergistic utilization of xylose and methanol was observed in the FFA producing strain. Finally, a mixture of glucose, xylose and methanol was evaluated for FFA production (1.2 g/L). This study showed that O. polymorpha is an ideal host for chemical production from various carbon sources.
Yunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J Zhou

2479 related Products with: Production of free fatty acids from various carbon sources by Ogataea polymorpha.

1,000 tests100tests1001KG500gm1kg100gm500g50gm

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#38647822   2022/05/26 To Up

Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals.
Peng Cai, Yunxia Li, Xiaoxin Zhai, Lun Yao, Xiaojun Ma, Lingyun Jia, Yongjin J Zhou

2960 related Products with: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

1 mg100 µg1 mL100ug100ug100ug Lyophilized500 1mg2 mg100.00 ug0.5 mg200

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#38647771   2022/10/22 To Up

Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

Ilamycins E/E are novel cyclic heptapeptides from Streptomyces atratus SCSIO ZH16, which have the MIC value of 9.8 nM against Mycobacterium tuberculosis H37Rv. However, the lower fermentative titer of ilamycins E/E cut off further development for novel anti-TB lead drugs. In order to break the obstacle, the combinatorial strategy of medium optimization, fermentative parameters optimization, exogenous addition of metal ions, precursors, and surfactants was developed to promoted the production of ilamycins E/E. Addition of 1 mM ZnCl at 0 h, 1 g/L tyrosine at 96 h, and 2 g/L shikimic acid at 48 h increased the production of ilamycins E/E from 13.51 to 762.50 ± 23.15, 721.39 ± 19.13, and 693.83 ± 16.86 mg/L, respectively. qRT-PCR results showed that the transcription levels of key genes in Embden-Meyerhof-Parnas pathway, hexose phosphate shunt pathway, and shikimic acid pathway were upregulated. In addition, the production of ilamycins E/E reached 790.34 mg/L in a 5-L bioreactor by combinatorial strategy. Combinatorial strategies were used for improving ilamycins E/E production in S. atratus ΔilaR and provided a sufficient basis on further clinic development.
Yunfei Zhu, Gaofan Zheng, Xiujuan Xin, Junying Ma, Jianhua Ju, Faliang An

2184 related Products with: Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

1 mg100μg0.1ml (1mg/ml)500 500 μg500 100ug100ug Lyophilized100ug100ug Lyophilized1 mL100μg

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#38647589   2022/05/18 To Up

From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.

With the increase in population growth and environmental pollution, the daily protein supply is facing great challenges. Single-cell protein (SCP) produced by microorganism fermentation is a good alternative for substituting plant- and animal-derived proteins. In this study, Paracoccus communis MA5 isolated from soil previously demonstrated an excellent ability to synthesize SCP directly from sodium formate. To investigate the central metabolic network of formic acid assimilation and protein synthesis, genome-scale analyses were performed. Genomic analysis showed that complete tetrahydrofolate cycle-, serine cycle-, glycolytic pathway-, tricarboxylic acid (TCA) cycle- and nitrogen metabolism-relevant genes were annotated in the genome. These pathways play key roles in the conversion of formic acid into proteins. Transcriptional analysis showed that sodium formate stress could stimulate the metabolic pathway in response to environmental stress, but weaken the sulfur metabolic pathway to inhibit amino acid synthesis, resulting in a decrease in protein content (30% vs 44%). However, under culture conditions with ammonium sulfate, metabolic pathways associated with protein synthesis were accelerated, causing an increase in protein content (53% vs 44%); while the tetrahydrofolate cycle associated with formic acid assimilation was inhibited, causing a 62.5% decrease in growth rate (OD: 0.21 vs 0.56). These results provide evidence of protein synthesis from sodium formate in strain MA5 at the gene level and lay a theoretical foundation for the optimization of fermentation systems using formic acid as a carbon source.
Sheng Tong, Lizhi Zhao, Daling Zhu, Wuxi Chen, Limei Chen, Demao Li

1648 related Products with: From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.

10 500 gm.0.5mg 100ul24 reactions 100ug Lyophilized200 ug1 mg100 ug1.00 flask

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#38647583   2022/08/29 To Up

Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.

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Rui Zhou, Sheng Dong, Yingang Feng, Qiu Cui, Jinsong Xuan

2711 related Products with: Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.

2 Pieces/Box2 Pieces/Box96T 5 G2 Pieces/Box1 ml2 Pieces/Box

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#38647569   2022/07/18 To Up

Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.

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Yue Ma, Tianzhen Li, Zijian Tan, Long Ma, Haifeng Liu, Leilei Zhu

1790 related Products with: Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.

100Tests 100 G 25 G10 mg10 mg100 MG 100 G500 mg1 kg 1 G 1KG

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#38647131   2024/04/22 To Up

Metabolic characterization of sphere-derived prostate cancer stem cells reveals aberrant urea cycle in stemness maintenance.

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Yuanyuan Luo, Jiachuan Yu, Zhikun Lin, Xiaolin Wang, Jinhui Zhao, Xinyu Liu, Wangshu Qin, Guowang Xu

1256 related Products with: Metabolic characterization of sphere-derived prostate cancer stem cells reveals aberrant urea cycle in stemness maintenance.

1 mg

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#38647109   2024/04/22 To Up

High-density generation of spatial transcriptomics with STAGE.

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Shang Li, Kuo Gai, Kangning Dong, Yiyang Zhang, Shihua Zhang

1456 related Products with: High-density generation of spatial transcriptomics with STAGE.

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#38647075   // To Up

Simple, sensitive, and visual detection of 12 respiratory pathogens with one-pot-RPA-CRISPR/Cas12a assay.

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Qi Tan, Yaoqiang Shi, Chenlu Duan, Qingyuan Li, Tao Gong, Shilin Li, Xiaoqiong Duan, He Xie, Yujia Li, Limin Chen

1997 related Products with: Simple, sensitive, and visual detection of 12 respiratory pathogens with one-pot-RPA-CRISPR/Cas12a assay.

100tests50 mg10 plates50 Test100tests100tests96 tests100tests100tests100tests

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#38647045   2024/04/22 To Up

Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.

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Lorenzo Olivi, Cleo Bagchus, Victor Pool, Ezra Bekkering, Konstantin Speckner, Hidde Offerhaus, Wen Y Wu, Martin Depken, Koen J A Martens, Raymond H J Staals, Johannes Hohlbein

1882 related Products with: Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.

100 TESTS 5 G100 stainings 5 G25 Tests100ug2.5 mg0.1ml (1mg/ml)25 mg100ug1 kit

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