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Search results for: 4-Amino-3-methylbutyric Acid Heminaphthalene-1,5-disulfonate 2C5H11NO2•C10H8O6S2 CAS: 1216629-00-3

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#33984647   2021/04/26 To Up

Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography.

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Milada Vítová, Milena Stránská, Andrea Palyzová, Tomáš Řezanka

2837 related Products with: Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography.

25 mg50 ul0.2 mg 5 G10 ug100ul100 assays 5 G 5 G50mg1 g

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#33984608   2021/05/02 To Up

Biochemical and metabolic responses of the deep-sea mussel Bathymodiolus platifrons to cadmium and copper exposure.

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Li Zhou, Mengna Li, Zhaoshan Zhong, Hao Chen, Xiaocheng Wang, Minxiao Wang, Zheng Xu, Lei Cao, Chao Lian, Huan Zhang, Hao Wang, Yan Sun, Chaolun Li

2378 related Products with: Biochemical and metabolic responses of the deep-sea mussel Bathymodiolus platifrons to cadmium and copper exposure.

25 MG100ul25 mg5mg100 mg200ug10 mg1 ml1000 tests100ug200ul

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#33984382   2021/05/10 To Up

Prolonged release and shelf-life of anticoagulant sulfated polysaccharides encapsulated with ZIF-8.

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Jie Zheng, Bingzhi Li, Yuan Ji, Yin Chen, Xun Lv, Xing Zhang, Robert J Linhardt

2784 related Products with: Prolonged release and shelf-life of anticoagulant sulfated polysaccharides encapsulated with ZIF-8.

100tests100tests250tests100tests100tests100ug5/100 Packing /sleeve/bo500 MG100 rxns 25 mg1000 TESTS/0.65ml10 mg

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#33980103   // To Up

Effect of amino acids and amines on the activity of the recombinant ι-carbonic anhydrase from the Gram-negative bacterium .

We here report a study on the activation of the ι-class bacterial CA from (BteCAι). This protein was recently characterised as a zinc-dependent enzyme that shows a significant catalytic activity ( 3.0 × 10 s) for the physiological reaction of CO hydration to bicarbonate and protons. Some amino acids and amines, among which some proteinogenic derivatives as well as histamine, dopamine and serotonin, showed efficient activating properties towards BteCAι, with activation constants in the range 3.9-13.3 µM. L-Phe, L-Asn, L-Glu, and some pyridyl-alkylamines, showed a weaker activating effect towards BteCAι, with values ranging between 18.4 µM and 45.6 µM. Nowadays, no information is available on active site architecture, metal ion coordination and catalytic mechanism of members of the ι-group of CAs, and this study represents another contribution towards a better understanding of this still uncharacterised class of enzymes.
Viviana De Luca, Andrea Petreni, Vincenzo Carginale, Andrea Scaloni, Claudiu T Supuran, Clemente Capasso

1029 related Products with: Effect of amino acids and amines on the activity of the recombinant ι-carbonic anhydrase from the Gram-negative bacterium .

1mg500IU50 IU10200 units2200 units2000 IU100 IU11 ml

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#33980009   2021/05/13 To Up

Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor.

The development of a sensing platform with high sensitivity and specificity, especially programmability and universal applicability, for the detection of clinically relevant molecules is highly valuable for disease monitoring and confirmation but remains a challenge. Here, for the first time, we introduce the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system into an immobilization-free electrochemical biosensing platform for sensitively and specifically detecting the disease-related nucleic acids and small molecules. In this strategy, a modular rolling circle amplification (RCA) is designed to transform and amplify the target recognition event into the universal trigger DNA strand that is used as the trigger to activate the deoxyribonuclease activity of CRISPR/Cas12a for further signal amplification. The cleavage of the target-activated blocker probe allows the methylene blue-labeled reporter probes to be captured by the reduced graphene oxide-modified electrode, leading to an obviously increased electrochemical signal. We only need to simply tune the sequence for target recognition in RCA components, and this strategy can be flexibly applied to the highly sensitive and specific detection of microRNAs, Parvovirus B19 DNA, and adenosine-5'-triphosphate and the calculated limit of detection is 0.83 aM, 0.52 aM, and 0.46 pM, respectively. In addition, we construct DNA logic circuits (YES, NOT, OR, AND) of DNA inputs to experimentally demonstrate the modularity and programmability of the stimuli-responsive RCA-CRISPR/Cas12a system. This work broadens the application of the CRISPR/Cas12a system to the immobilization-free electrochemical biosensing platform and provides a new thinking for developing a robust tool for clinical diagnosis.
Min Qing, Sheng Liang Chen, Zhe Sun, Yi Fan, Hong Qun Luo, Nian Bing Li

1162 related Products with: Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor.

1 ml25500 MG1 mg500g25 mg1 mg1 mg1 mg500gm

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#33978171   // To Up

Progress of genome editing technology and developmental biology useful for radiation research.

Following the development of genome editing technology, it has become more feasible to create genetically modified animals such as knockout (KO), knock-in, and point-mutated animals. The genome-edited animals are useful to investigate the roles of various functional genes in many fields of biological science including radiation research. Nevertheless, some researchers may experience difficulty in generating genome-edited animals, probably due to the requirement for equipment and techniques for embryo manipulation and handling. Furthermore, after obtaining F0 generation, genome-edited animals generally need to be expanded and maintained for analyzing the target gene function. To investigate genes essential for normal birth and growth, the generation of conditional KO (cKO) animals in which a tissue- or stage-specific gene mutation can be introduced is often required. Here, we describe the basic principle and application of genome editing technology including zinc-finger nuclease, transcription-activator-like effector nuclease, and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associated protein (Cas) systems. Recently advanced developmental biology methods have enabled application of the technology, especially CRISPR/Cas, to zygotes, leading to the prompt production of genome-edited animals. For pre-implantation embryos, genome editing via oviductal nucleic acid delivery has been developed as an embryo manipulation- or handling-free method. Examining the gene function at F0 generation is becoming possible by employing triple-target CRISPR technology. This technology, in combination with a blastocyst complementation method enables investigation of even birth- and growth-responsible genes without establishing cKO strains. We hope that this review is helpful for understanding and expanding genome editing-related technology and for progressing radiation research.
Kento Miura, Atsuo Ogura, Kohei Kobatake, Hiroaki Honda, Osamu Kaminuma

2824 related Products with: Progress of genome editing technology and developmental biology useful for radiation research.

1L 2x5L25 μg100Tests500 grams200ul5 mg 500 G25 mg

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#33977072   2021/03/01 To Up

Construction of an Alternative NAD De Novo Biosynthesis Pathway.

Nicotinamide adenine dinucleotide (NAD) is a life essential molecule involved in versatile biological processes. To date, only two de novo biosynthetic routes to NAD are described, both of which start from a proteinogenic amino acid and are tightly controlled. Here, a de novo quinolinic acid pathway starting from chorismate, which provides an alternative route (named as the C3N pathway) to NAD biosynthesis, is established. Significantly, the C3N pathway yields extremely high cellular concentrations of NAD(H) in . Its utility in cofactor engineering is demonstrated by introducing the four-gene C3N module to cell factories to achieve higher production of 2,5-dimethylpyrazine and develop an efficient C3N-based whole-cell bioconversion system for preparing chiral amines. The wide distribution and abundance of chorismate in most kingdoms of life implies a general utility of the C3N pathway for modulating cellular levels of NAD(H) in versatile organisms.
Yong Ding, Xinli Li, Geoff P Horsman, Pengwei Li, Min Wang, Jine Li, Zhilong Zhang, Weifeng Liu, Bian Wu, Yong Tao, Yihua Chen

2831 related Products with: Construction of an Alternative NAD De Novo Biosynthesis Pathway.

100ul 100ul2 Pieces/Box100ul100ug Lyophilized100ug Lyophilized8 Array supply x 2100.00 ug100ug Lyophilized100ug Lyophilized100.00 ul100ug Lyophilized

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#33976107   2020/05/12 To Up

Characterization of a novel type III CRISPR-Cas effector provides new insights into the allosteric activation and suppression of the Cas10 DNase.

Antiviral defense by type III CRISPR-Cas systems relies on two distinct activities of their effectors: the RNA-activated DNA cleavage and synthesis of cyclic oligoadenylate. Both activities are featured as indiscriminate nucleic acid cleavage and subjected to the spatiotemporal regulation. To yield further insights into the involved mechanisms, we reconstituted LdCsm, a lactobacilli III-A system in Escherichia coli. Upon activation by target RNA, this immune system mediates robust DNA degradation but lacks the synthesis of cyclic oligoadenylates. Mutagenesis of the Csm3 and Cas10 conserved residues revealed that Csm3 and multiple structural domains in Cas10 function in the allosteric regulation to yield an active enzyme. Target RNAs carrying various truncations in the 3' anti-tag were designed and tested for their influence on DNA binding and DNA cleavage of LdCsm. Three distinct states of ternary LdCsm complexes were identified. In particular, binding of target RNAs carrying a single nucleotide in the 3' anti-tag to LdCsm yielded an active LdCsm DNase regardless whether the nucleotide shows a mismatch, as in the cognate target RNA (CTR), or a match, as in the noncognate target RNA (NTR), to the 5' tag of crRNA. In addition, further increasing the number of 3' anti-tag in CTR facilitated the substrate binding and enhanced the substrate degradation whereas doing the same as in NTR gradually decreased the substrate binding and eventually shut off the DNA cleavage by the enzyme. Together, these results provide the mechanistic insights into the allosteric activation and repression of LdCsm enzymes.
Jinzhong Lin, Mingxia Feng, Heping Zhang, Qunxin She

1735 related Products with: Characterization of a novel type III CRISPR-Cas effector provides new insights into the allosteric activation and suppression of the Cas10 DNase.



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