Search results for: (3S,4S)-4-Amino-1-oxyl-2,2,6,6-(3R,4R)-tetramethylpiperidine-3-carboxylic Acid C10H19N2O3 CAS: 691364-98-4
#38647893 
2022/07/30
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Production of free fatty acids from various carbon sources by Ogataea polymorpha.
EYunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J Zhou
1745 related Products with: Production of free fatty acids from various carbon sources by Ogataea polymorpha.
1,000 tests100tests1001KG500gm1kg100gm500g50gm
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#38647822 2022/05/26
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Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.
�Peng Cai, Yunxia Li, Xiaoxin Zhai, Lun Yao, Xiaojun Ma, Lingyun Jia, Yongjin J Zhou
1494 related Products with: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.
1 mg100 µg1 mL100ug100ug6 ml100ug Lyophilized500 1mg2 mg100.00 ug0.5 mg
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#38647771 2022/10/22
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Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.
IYunfei Zhu, Gaofan Zheng, Xiujuan Xin, Junying Ma, Jianhua Ju, Faliang An
1745 related Products with: Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.
1 mg100μg0.1ml (1mg/ml)500 500 500 μg100ug100ug Lyophilized100ug1 mL100ug Lyophilized100μg
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#38647589 2022/05/18
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From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.
With the increase in population growth and environmental pollution, the daily protein supply is facing great challenges. Single-cell protein (SCP) produced by microorganism fermentation is a good alternative for substituting plant- and animal-derived proteins. In this study, Paracoccus communis MA5 isolated from soil previously demonstrated an excellent ability to synthesize SCP directly from sodium formate. To investigate the central metabolic network of formic acid assimilation and protein synthesis, genome-scale analyses were performed. Genomic analysis showed that complete tetrahydrofolate cycle-, serine cycle-, glycolytic pathway-, tricarboxylic acid (TCA) cycle- and nitrogen metabolism-relevant genes were annotated in the genome. These pathways play key roles in the conversion of formic acid into proteins. Transcriptional analysis showed that sodium formate stress could stimulate the metabolic pathway in response to environmental stress, but weaken the sulfur metabolic pathway to inhibit amino acid synthesis, resulting in a decrease in protein content (30% vs 44%). However, under culture conditions with ammonium sulfate, metabolic pathways associated with protein synthesis were accelerated, causing an increase in protein content (53% vs 44%); while the tetrahydrofolate cycle associated with formic acid assimilation was inhibited, causing a 62.5% decrease in growth rate (OD: 0.21 vs 0.56). These results provide evidence of protein synthesis from sodium formate in strain MA5 at the gene level and lay a theoretical foundation for the optimization of fermentation systems using formic acid as a carbon source.Sheng Tong, Lizhi Zhao, Daling Zhu, Wuxi Chen, Limei Chen, Demao Li
1912 related Products with: From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.
10 500 gm.1 mg 25 ml 100 ug1.00 flask 100ul200 ug100ug Lyophilized
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#38647583 2022/08/29
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Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.
BRui Zhou, Sheng Dong, Yingang Feng, Qiu Cui, Jinsong Xuan
1643 related Products with: Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.
2 Pieces/Box2 Pieces/Box96T 5 G2 Pieces/Box1 ml2 Pieces/Box
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#38647569 2022/07/18
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Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.
CYue Ma, Tianzhen Li, Zijian Tan, Long Ma, Haifeng Liu, Leilei Zhu
2573 related Products with: Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.
100Tests 100 G 25 G10 mg10 mg100 MG 100 G500 mg1 kg 1KG 1 G
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#38647131 2024/04/22
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Metabolic characterization of sphere-derived prostate cancer stem cells reveals aberrant urea cycle in stemness maintenance.
AYuanyuan Luo, Jiachuan Yu, Zhikun Lin, Xiaolin Wang, Jinhui Zhao, Xinyu Liu, Wangshu Qin, Guowang Xu
1277 related Products with: Metabolic characterization of sphere-derived prostate cancer stem cells reveals aberrant urea cycle in stemness maintenance.
1 mg
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#38647109 2024/04/22
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High-density generation of spatial transcriptomics with STAGE.
SShang Li, Kuo Gai, Kangning Dong, Yiyang Zhang, Shihua Zhang
2725 related Products with: High-density generation of spatial transcriptomics with STAGE.
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#38647075 //
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Simple, sensitive, and visual detection of 12 respiratory pathogens with one-pot-RPA-CRISPR/Cas12a assay.
RQi Tan, Yaoqiang Shi, Chenlu Duan, Qingyuan Li, Tao Gong, Shilin Li, Xiaoqiong Duan, He Xie, Yujia Li, Limin Chen
2025 related Products with: Simple, sensitive, and visual detection of 12 respiratory pathogens with one-pot-RPA-CRISPR/Cas12a assay.
100tests50 mg10 plates100tests100tests50 Test96 tests100tests100tests100tests
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#38647045 2024/04/22
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Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.
CRISPR-Cas systems have widely been adopted as genome editing tools, with two frequently employed Cas nucleases being SpyCas9 and LbCas12a. Although both nucleases use RNA guides to find and cleave target DNA sites, the two enzymes differ in terms of protospacer-adjacent motif (PAM) requirements, guide architecture and cleavage mechanism. In the last years, rational engineering led to the creation of PAM-relaxed variants SpRYCas9 and impLbCas12a to broaden the targetable DNA space. By employing their catalytically inactive variants (dCas9/dCas12a), we quantified how the protein-specific characteristics impact the target search process. To allow quantification, we fused these nucleases to the photoactivatable fluorescent protein PAmCherry2.1 and performed single-particle tracking in cells of Escherichia coli. From our tracking analysis, we derived kinetic parameters for each nuclease with a non-targeting RNA guide, strongly suggesting that interrogation of DNA by LbdCas12a variants proceeds faster than that of SpydCas9. In the presence of a targeting RNA guide, both simulations and imaging of cells confirmed that LbdCas12a variants are faster and more efficient in finding a specific target site. Our work demonstrates the trade-off of relaxing PAM requirements in SpydCas9 and LbdCas12a using a powerful framework, which can be applied to other nucleases to quantify their DNA target search.Lorenzo Olivi, Cleo Bagchus, Victor Pool, Ezra Bekkering, Konstantin Speckner, Hidde Offerhaus, Wen Y Wu, Martin Depken, Koen J A Martens, Raymond H J Staals, Johannes Hohlbein