Search results for: (-)-2-Amino-6-propionamido-d3-tetrahydrobenzothiazole C10H12D3N3OS CAS:
#38652440 
2024/04/23
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CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety.
The development of a bio-sensing strategy based on CRISPR/Cas that is exceptionally sensitive is crucial for the identification of trace molecules. Colorimetric miRNA detection utilizing CRISPR/Cas13a-triggered DNAzyme signal amplification was described in this article. The developed strategy was implemented for miRNA-21 detection as a proof of concept. The cleavage activity of Cas13a was triggered when the target molecule bonded to the Cas13a-crRNA complex and cleaved uracil ribonucleotides (rU) in the substrate probe. As a consequence, the S chain was liberated from the T chain that had been modified on magnetic beads (MB). The G-rich sections were then exposed when the catalytic hairpin assembly between the H1 and H2 probes was activated by the released T@MB. G-rich section can fold into G-quadruplex. By catalyzing the formation of green ABTS- via HRP-mimicking G-quadruplex/hemin complexes, colorimetric measurements of miRNA can be achieved visually through DNAzyme-mediated signal amplification. The method demonstrated a low limit of detection of 27 fM and a high selectivity towards target miRNA eventually. As a result, the developed strategy provides a clinical application platform for the detection of miRNAs that is both ultrasensitive and extremely specific.Na Yan, Zhiwei Hu, Lingling Zhang
1380 related Products with: CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety.
100tests100tests100tests100tests100tests100tests100tests100tests100tests100tests100tests 100ul
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#38652321 2024/04/23
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Advances in miniature CRISPR-Cas proteins and their applications in gene editing.
The CRISPR-Cas system consists of Cas proteins and single-stranded RNAs that recruit Cas proteins and specifically target the nucleic acid. Some Cas proteins can accurately cleave the target nucleic acid under the guidance of the single-stranded RNAs. Due to its exceptionally high specificity, the CRISPR-Cas system is now widely used in various fields such as gene editing, transcription regulation, and molecular diagnosis. However, the huge size of the most frequently utilized Cas proteins (Cas9, Cas12a, and Cas13, which contain 950-1,400 amino acids) can limit their applicability, especially in eukaryotic gene editing, where larger Cas proteins are difficult to deliver into the target cells. Recently discovered miniature CRISPR-Cas proteins, consisting of only 400 to 800 amino acids, offer the possibility of overcoming this limitation. This article systematically reviews the latest research progress of several miniature CRISPR-Cas proteins (Cas12f, Cas12j, Cas12k, and Cas12m) and their practical applications in the field of gene editing.Huimin Wu, Yixiang Sun, Yimai Wang, Liqiang Luo, Yizhi Song
2185 related Products with: Advances in miniature CRISPR-Cas proteins and their applications in gene editing.
101 Set100 7 x 25 ul
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#38652294 2024/04/23
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Plant diversity increases spatial stability of aboveground productivity in alpine grasslands.
Plant diversity can significantly affect the grassland productivity and its stability. However, it remains unclear how plant diversity affects the spatial stability of natural grassland productivity, especially in alpine regions that are sensitive to climate change. We analyzed the interaction between plant (species richness and productivity, etc.) and climatic factors (precipitation, temperature, and moisture index, etc.) of alpine natural grassland on the Qinghai-Tibetan Plateau. In addition, we tested the relationship between plant diversity and spatial stability of grassland productivity. Results showed that an increase in plant diversity significantly enhanced community productivity and its standard deviation, while reducing the coefficient of variation in productivity. The influence of plant diversity on productivity and the reciprocal of productivity variability coefficient was not affected by vegetation types. The absolute values of the regression slopes between climate factors and productivity in alpine meadow communities with higher plant diversity were smaller than those in alpine meadow communities with lower plant diversity. In other words, alpine meadow communities with higher plant diversity exhibited a weaker response to climatic factors in terms of productivity, whereas those with lower plant diversity showed a stronger response. Our results indicate that high plant diversity buffers the impact of ambient pressure (e.g., precipitation, temperature) on alpine meadow productivity, and significantly enhanced the spatial stability of grassland productivity. This finding provides a theoretical basis for maintaining the stability of grassland ecosystems and scientifically managing alpine grasslands under the continuous climate change.Zeng Cui, Jian Sun, Gao-Lin Wu
2625 related Products with: Plant diversity increases spatial stability of aboveground productivity in alpine grasslands.
4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo96tests4/120 Packing /sleeve/bo1 Set1 Set5 mg 0.1 mg
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#38652293 2024/04/23
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Intraspecific alternative phenotypes contribute to variation in species' strategies for growth.
ESamantha J Worthy, María N Umaña, Caicai Zhang, Luxiang Lin, Min Cao, Nathan G Swenson
1017 related Products with: Intraspecific alternative phenotypes contribute to variation in species' strategies for growth.
100 μg 100ul25 µg4 Membranes/Box100 ml50 ug 96T 100ul1 kit96T 100 G
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#38652189 2024/04/23
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Siderophore-producing bacteria from Spitsbergen soils-novel agents assisted in bioremediation of the metal-polluted soils.
SMałgorzata Majewska, Anna Słomka, Agnieszka Hanaka
1892 related Products with: Siderophore-producing bacteria from Spitsbergen soils-novel agents assisted in bioremediation of the metal-polluted soils.
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#38652094 2024/04/23
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Sulfonic Acid Functionalized Ionic Liquids for Defect Passivation via Molecular Interactions for High-Quality Perovskite Films and Stable Solar Cells.
The high photoelectric conversion efficiency and low cost of perovskite solar cells (PSCs) have further inspired people's determination to push this technology toward industrialization. The high-quality perovskite films and high-efficiency and stable PSCs are the crucial factors. Ionic liquids have been proven to be an effective strategy for regulating high-quality perovskite films and high-performance PSCs. However, the regulation mechanism between ionic liquids and perovskites still needs further clarification. In this study, a novel sulfonic acid-functionalized ionic liquid, 1-butyl-3-methylimidazolium trifluoromethanesulfonate (BSOHMImOTf), was used as an effective additive to regulate high-quality perovskite films and high-performance devices. Microscopic mechanism studies revealed strong interactions between BSOHMImOTf and Pb ions as well as halogens in the perovskite. The perovskite film is effectively passivated with the controlled crystal growth, suppressed ion migration, facilitating to the greatly improved photovoltaic performance, and superior long-term stability. This article reveals the regulatory mechanism of sulfonic acid type ionic liquids through testing characterization and mechanism analysis, providing a new approach for the preparation of high-quality perovskite devices.Junhui Fang, Liang Wang, Zhaoyang Chen, Shuai Wang, Lin Yuan, Aamir Saeed, Iqbal Hussain, Jianwei Zhao, Ruixia Liu, Qingqing Miao
1433 related Products with: Sulfonic Acid Functionalized Ionic Liquids for Defect Passivation via Molecular Interactions for High-Quality Perovskite Films and Stable Solar Cells.
1 G 500 G 1 G20 ug10 mg 1 G25 g 1 G 2x5L5 g 100 G
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#38651920 2024/04/23
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Biomechanical effects of clear aligner with different shape design at extraction space area during anterior teeth retraction.
This study aimed to investigate the biomechanical effects of clear aligner (CA) with different shape designs at extraction space (CAES) area during space closing.Bochun Mao, Yajing Tian, Yujia Xiao, Jiayi Liu, Dawei Liu, Jing Li, Yanheng Zhou
1153 related Products with: Biomechanical effects of clear aligner with different shape design at extraction space area during anterior teeth retraction.
5100ug Lyophilized10 ug100ul100μl20 ul100 extractions100ug 50 MG100ul100 extractions1 ml
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#38651836 2024/04/23
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Machine Learning for Experimental Reactivity of a Set of Metal Clusters toward C-H Activation.
UXi-Guan Zhao, Qi Yang, Ying Xu, Qing-Yu Liu, Zi-Yu Li, Xiao-Xiao Liu, Yan-Xia Zhao, Sheng-Gui He
1145 related Products with: Machine Learning for Experimental Reactivity of a Set of Metal Clusters toward C-H Activation.
100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized 100ul100ug Lyophilized100ug Lyophilized100 μg100ug Lyophilized100ug Lyophilized100ug Lyophilized
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#38651620 2024/04/23
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Acceptor-Reactivity-Controlled Stereoconvergent Synthesis and Immunological Activity of a Unique Pentasaccharide from the Cell Wall Polysaccharide of Cutibacterium acnes C7.
BTianhui Hao, Ke Feng, Hongzhen Jin, Jiawei Li, Chenkai Zhou, Xingbang Liu, Wei Zhao, Fan Yu, Tiehai Li
2981 related Products with: Acceptor-Reactivity-Controlled Stereoconvergent Synthesis and Immunological Activity of a Unique Pentasaccharide from the Cell Wall Polysaccharide of Cutibacterium acnes C7.
1 kit100ug100 Tests100 Tests100ug Lyophilized1 kit100 Tests100ug Lyophilized
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#38651538 2024/04/23
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A wafer scale thin film of ultra-small ScO nanocrystals on a 2D COF with high rigidity.
Scandium oxide (ScO) has a wide range of applications in metallurgy, chemical industry, electronics and many other high-tech fields. However, most ScO materials exist in the powder or bulk form, while nanostructured ScO has rarely been reported as there is a lack of a common method to control its dimensionality, hindering the understanding of new properties and potential applications of nano-ScO materials. In this paper, we establish a procedure to synthesize a two-dimensional (2D) ScO-covalent organic framework (COF) composite film where the crystal size of ScO domains is as small as ∼3 nm. The composite film is prepared by a Schiff base condensation reaction at the sharp -pentane/water interface using a combination of surfactant-monolayer-assisted interfacial synthesis and laminar assembly polymerization methods. Then the conditions of nucleation and uniform film formation of the 2D ScO/COF are explored further. Meanwhile, an atomic force microscopy indentation test shows that the material has a high Young's modulus of 89.1 ± 3.8 GPa, which is much higher than those of the majority of reported 2D polymer materials. We further extended this synthesis method to the preparation of YbO (ytterbium oxide) and/or ErO (erbium oxide)-incorporated 2D COF composite films, verifying the universality of this strategy. This work provides an opportunity to vary the dimensionality of many kinds of metal oxides and explore the potential applications of low-dimensional ScO materials.Xin Guan, Xiaohui Xu, Zhongliang Yu, Junjie Xiong, Yanhong Chang, Bowen Liu, Bin Wang