Gentaur Pub

#38648296   2024/04/22 To Up

Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.

The delivery of proteins into the cytosol holds great promise for cell signaling manipulation and the development of precision medicine. However, this potency is challenged by achieving targeted and controlled delivery, specifically within diseased cells. In this study, we introduce a versatile and effective method for the precision delivery of therapeutic proteins to cancer cells by designing polyphenol-assisted biomineralization of zeolite imidazole framework-8 (ZIF-8). We demonstrate that by leveraging the strong noncovalent binding affinity of epigallocatechin gallate (EGCG) with both proteins and ZIF-8, our approach significantly enhances the biomineralization of ZIF-8, which in turn improves the efficiency of protein encapsulation and intracellular delivery. Moreover, the incorporation of EGCG within ZIF-8 enables controlled degradation of the nanoparticles and the selective release of the encapsulated proteins in cancer cells. This selective release is triggered by the oxidation of EGCG in response to the high levels of reactive oxygen species (ROS) found within cancer cells that destabilize the EGCG/ZIF-8 nanoparticles. We have further demonstrated the ability of EGCG/ZIF-8 to deliver a wide range of proteins into cancer cells, including bacterial virulence protein, to rewire cell signaling and prohibit tumor cell growth in a mouse xenograft model. Our strategy and findings underscore the potential of designing the EGCG/ZIF-8 interface for specific and controlled protein delivery for targeted cancer therapy.
Tianli Luo, Qizhen Zheng, Ji Liu, Rui Yao, Ming Wang

2618 related Products with: Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.

1mg10501mg1001 mg2101.00 flask100

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#38647965   2023/09/15 To Up

Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications.

In the context of the rapid development of low-carbon economy, there has been increasing interest in utilizing naturally abundant and cost-effective one-carbon (C1) substrates for sustainable production of chemicals and fuels. Moorella thermoacetica, a model acetogenic bacterium, has attracted significant attention due to its ability to utilize carbon dioxide (CO) and carbon monoxide (CO) via the Wood-Ljungdahl (WL) pathway, thereby showing great potential for the utilization of C1 gases. However, natural strains of M. thermoacetica are not yet fully suitable for industrial applications due to their limitations in carbon assimilation and conversion efficiency as well as limited product range. Over the past decade, progresses have been made in the development of genetic tools for M. thermoacetica, accelerating the understanding and modification of this acetogen. Here, we summarize the physiological and metabolic characteristics of M. thermoacetica and review the recent advances in engineering this bacterium. Finally, we propose the future directions for exploring the real potential of M. thermoacetica in industrial applications.
Dechen Jia, Wangshuying Deng, Peng Hu, Weihong Jiang, Yang Gu

2800 related Products with: Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications.

250 ml5 g0.2 mg10 mg25 mg10 mg0.1 mg10 mg1 mg1 LITRE96 Tests1 ml

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#38647893   2022/07/30 To Up

Production of free fatty acids from various carbon sources by Ogataea polymorpha.

Energy shortage and environmental concern urgently require establishing the feasible bio-refinery process from various feedstocks. The methylotrophic yeast Ogataea polymorpha is thermo-tolerant and can utilize various carbon sources, such as glucose, xylose and methanol, which makes it a promising host for bio-manufacturing. Here, we explored the capacity of O. polymorpha for overproduction of free fatty acids (FFAs) from multiple substrates. The engineered yeast produced 674 mg/L FFA from 20 g/L glucose in shake flask and could sequentially utilize the mixture of glucose and xylose. However, the FFA producing strain failed to survive in sole methanol and supplementing co-substrate xylose promoted methanol metabolism. A synergistic utilization of xylose and methanol was observed in the FFA producing strain. Finally, a mixture of glucose, xylose and methanol was evaluated for FFA production (1.2 g/L). This study showed that O. polymorpha is an ideal host for chemical production from various carbon sources.
Yunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J Zhou

2872 related Products with: Production of free fatty acids from various carbon sources by Ogataea polymorpha.

1,000 tests100tests1001KG500gm1kg100gm500g50gm

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#38647886   2023/06/28 To Up

Construction of Cupriavidus necator displayed with superoxide dismutases for enhanced growth in bioelectrochemical systems.

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Ke Chen, Chunling Ma, Xiaolei Cheng, Yuhua Wang, Kun Guo, Ranran Wu, Zhiguang Zhu

1429 related Products with: Construction of Cupriavidus necator displayed with superoxide dismutases for enhanced growth in bioelectrochemical systems.

20ug500 MG2 Pieces/Box100.00 ug10ug100.00 ug0.1ml (1mg/ml)10ug 100 G100.00 ug

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#38647878   2023/03/02 To Up

Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.

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Shiyu Liang, Yue Zhang, Liting Lyu, Shuang Wang, Zongbao K Zhao

1624 related Products with: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.

1 G 1 G100 mg300 units 5 G10 mg

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#38647771   2022/10/22 To Up

Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

Ilamycins E/E are novel cyclic heptapeptides from Streptomyces atratus SCSIO ZH16, which have the MIC value of 9.8 nM against Mycobacterium tuberculosis H37Rv. However, the lower fermentative titer of ilamycins E/E cut off further development for novel anti-TB lead drugs. In order to break the obstacle, the combinatorial strategy of medium optimization, fermentative parameters optimization, exogenous addition of metal ions, precursors, and surfactants was developed to promoted the production of ilamycins E/E. Addition of 1 mM ZnCl at 0 h, 1 g/L tyrosine at 96 h, and 2 g/L shikimic acid at 48 h increased the production of ilamycins E/E from 13.51 to 762.50 ± 23.15, 721.39 ± 19.13, and 693.83 ± 16.86 mg/L, respectively. qRT-PCR results showed that the transcription levels of key genes in Embden-Meyerhof-Parnas pathway, hexose phosphate shunt pathway, and shikimic acid pathway were upregulated. In addition, the production of ilamycins E/E reached 790.34 mg/L in a 5-L bioreactor by combinatorial strategy. Combinatorial strategies were used for improving ilamycins E/E production in S. atratus ΔilaR and provided a sufficient basis on further clinic development.
Yunfei Zhu, Gaofan Zheng, Xiujuan Xin, Junying Ma, Jianhua Ju, Faliang An

2121 related Products with: Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

1 mg100μg0.1ml (1mg/ml)500 500 μg500 100ug100ug Lyophilized100ug100ug Lyophilized1 mL100μg

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