Search results for: AR
#33108824 2020/10/27 To Up
Structural analysis of the impact of a novel androgen receptor gene mutation in two adult patients with mild androgen insensitivity syndrome.Androgen receptor gene (AR) mutations are responsible for androgen insensitivity syndrome (AIS) presenting with a clinical phenotype that ranges from gynaecomastia and/ or infertility in mild AIS (MAIS) to complete testicular feminisation in complete AIS. We report a novel AR gene mutation in two unrelated adult patients with MAIS and we studied its functional impact using 3D modelling. Patient 1, referred for infertility, presented with gynaecomastia, mild hypospadias and bilateral testicular hypotrophy contrasting with high testosterone levels, an elevated FSH, an elevated androgen sensitivity index (ASI) and oligoasthenoteratospermia. In vitro fertilisation and intracytoplasmic sperm injection resulted in a successful twin pregnancy. Patient 2 referred for a decrease in athletic performance had surgically treated gynaecomastia, oligoasthenospermia, high testosterone levels and an elevated ASI. Despite his impaired spermogram, he fathered two children without assisted reproductive technology. AR gene sequencing in the two patients revealed a common novel missense mutation, Ala699Thr, in exon 4 within the ligand-binding domain. 3D modelling studies showed that this mutation may impact dimer stability upon ligand binding or may affect allosteric changes upon dimerisation. This study illustrates the value of structural analysis for the functional study of mutations and expands the database of AR gene mutations.
Mirella Hage, Delphine Drui, Bruno Francou, Sandra Mercier, Anne Guiochon-Mantel, Joelle Belaisch-Allart, Yann Péréon, Laure Cazabat, Philippe De Mazancourt, Marie Laure Raffin-Sanson
2880 related Products with: Structural analysis of the impact of a novel androgen receptor gene mutation in two adult patients with mild androgen insensitivity syndrome.50 ug 200ug1000 100ul200ul100ug100ug0.1 mg50 ug 200ug200ul100ug
#33108427 2020/10/27 To Up
Molecular motion in the nanospace of MOFs upon gas adsorption investigated by Raman spectroscopy.Molecular motions taking place in the nanospace of metal-organic frameworks (MOFs) are an interesting research subject, although not yet fully investigated. In this work, we utilized in situ Raman spectroscopy in the ultralow-frequency region to investigate the libration motion (including the rotational motion of phenylene rings) of MOFs, in particular [Cu2(bdc)2(dabco)] (Cu-JAST-1), where bdc = 1,4-benzenedicarboxylate and dabco = 1,4-diazabicyclo[2.2.2]octane. The libration mode of Cu-JAST-1 was found to be significantly suppressed by the adsorption of various guest molecules, such as CO2, Ar, and N2. In addition, an appreciable correlation between the libration mode and adsorption equilibrium time was identified, which provides useful novel tools in the design of MOFs acting as molecular adsorption and separation materials.
Shinpei Kusaka, Yasuaki Nakajima, Akihiro Hori, Akira Yonezu, Kenta Kikushima, Wataru Kosaka, Yunsheng Ma, Ryotaro Matsuda
2882 related Products with: Molecular motion in the nanospace of MOFs upon gas adsorption investigated by Raman spectroscopy.250 TESTS1
#33107824 2020/10/27 To Up
PDGF/VEGF signaling from muscles to hepatocyte-like cells protects against obesity.PDGF/VEGF ligands regulate a plethora of biological processes in multicellular organisms via autocrine, paracrine and endocrine mechanisms. We investigated organ-specific metabolic roles of PDGF/VEGF-like factors (Pvfs). We combine genetic approaches and single-nuclei sequencing to demonstrate that muscle-derived Pvf1 signals to the hepatocyte-like cells/oenocytes to suppress lipid synthesis by activating the Pi3K/Akt1/TOR signaling cascade in the oenocytes. Functionally, this signaling axis regulates expansion of adipose tissue lipid stores in newly eclosed flies. Flies emerge after pupation with limited adipose tissue lipid stores and lipid level is progressively accumulated via lipid synthesis. We find that adult muscle-specific expression of increases rapidly during this stage and that muscle-to-oenocyte Pvf1 signaling inhibits expansion of adipose tissue lipid stores as the process reaches completion. Our findings provide the first evidence in a metazoan of a PDGF/VEGF ligand acting as a myokine that regulates systemic lipid homeostasis by activating TOR in hepatocyte-like cells.
Arpan C Ghosh, Sudhir Gopal Tattikota, Yifang Liu, Aram Comjean, Yanhui Hu, Victor Barrera, Shannan J Ho Sui, Norbert Perrimon
2247 related Products with: PDGF/VEGF signaling from muscles to hepatocyte-like cells protects against obesity.1.00 flask100 extractions21.5 x 10^6 cells1mg1.5x10(6) cells10100 ul 1KG50 ug 96 Tests n
#33107732 2020/10/27 To Up
Carbon Bond Breaking under Ar-Ion Irradiation in Dependence on sp Hybridization: Car-Parrinello, Ehrenfest, and Classical Dynamics Study.The modeling of low-temperature plasma synthesis of low-dimensional carbon coatings remains a challenge, since the long-time spans must be simulated for structural relaxation. A proper theoretical method should be chosen to address possible charge-transfer processes. Considering the possibility of linear-chained carbon (LCC) synthesis simulation, a numerical study of the C-C bond breaking under slow argon-ion irradiation is performed for the model molecules of 2,2,3,3-tetramethylbutane (sp hybridization), 1,2,3-butatriene (sp), and 2-butyne (sp). Threshold energies of bond breaking are calculated for the carbon atoms in all three hybridizations. Three levels of theory are applied, and the results are compared with experiment. Based on the accuracy of the values obtained, an approach is proposed for modeling the ion-assisted plasma synthesis.
Evgeny A Buntov, Anatoly F Zatsepin
1973 related Products with: Carbon Bond Breaking under Ar-Ion Irradiation in Dependence on sp Hybridization: Car-Parrinello, Ehrenfest, and Classical Dynamics Study.4 Arrays/Slide50ul4 Membranes/Box2 Pieces/Box4 Membranes/Box2 Pieces/Box5mg100 μg 25 G4 Arrays/Slide
#33107696 2020/10/27 To Up
Retrospective Analysis of Inpatient Polysomnogram Characteristics and Discharge Outcomes in Infants with Bronchopulmonary Dysplasia Requiring Home Oxygen Therapy.Little is known about the polysomnogram characteristics in infants with BPD, especially severe BPD, who do not need home ventilatory support but are at increased risk for chronic hypoxia and are vulnerable to its effects.
Nicole Flores-Fenlon, Noah Wright, Cheryl Lew, Theodora A Stavroudis, Choo Phei Wee, Sally L Davidson Ward, Manvi Bansal
1862 related Products with: Retrospective Analysis of Inpatient Polysomnogram Characteristics and Discharge Outcomes in Infants with Bronchopulmonary Dysplasia Requiring Home Oxygen Therapy.100 μg100 μg 100ul100 μg1 ml100 μg100 µg1.00 flask
#33107537 2020/10/27 To Up
and analysis of FAK/MMP signaling axis inhibition by VO-clioquinol in 2D and 3D human osteosarcoma cancer cells.The study of novel mechanisms of action of vanadium compounds is critical to elucidating the role and importance of these kinds of compounds as antitumor and antimetastatic agents. This work deals with in silico and in vitro studies of one clioquinol oxidovanadium(iv) complex [VO(clioquinol)2], VO(CQ)2, and its regulation of FAK. In particular, we focus on elucidating the relationship of the FAK inhibition, MMP activity and antimetastatic effects of the complex in human bone cancer cells.
Lucia M Balsa, Patricia Quispe, Enrique J Baran, Martin J Lavecchia, Ignacio E León
1933 related Products with: and analysis of FAK/MMP signaling axis inhibition by VO-clioquinol in 2D and 3D human osteosarcoma cancer cells.1 mg1.00 flask10 ug1.00 flask100ul200 1.00 flask1000 0.1 mg
#33107196 2020/10/26 To Up
Acetate: Friend or foe against breast tumour growth in the context of obesity?Acetate is reported as a regulator of fat mass but also as lipogenic source for cancer cells. Breast cancer is surrounded by adipose tissue and has been associated with obesity. However, whether acetate contributes to cancer cell metabolism as lipogenic substrate and/or by changing fat storage and eventually obesity-induced breast cancer progression remains unknown. Therefore, we studied the contribution of acetate to breast cancer metabolism and progression. In vitro, we found that acetate is not a bioenergetic substrate under normoxia and did not result in a significant change of growth. However, by using lipidomic approaches, we discovered that acetate changes the lipid profiles of the cells under hypoxia. Moreover, while mice fed a high-fat diet (HFD) developed bigger tumours than their lean counterparts, exogenous acetate supplementation leads to a complete abolishment of fat mass gain without reverting the HFD-induced obesity-driven tumour progression. In conclusion, although acetate protects against diet-induced obesity, our data suggest that it is not affecting HFD-driven tumour progression.
Caner Yelek, Lionel Mignion, Nicolas Joudiou, Romano Terrasi, Florian Gourgue, Matthias Van Hul, Nathalie Delzenne, Bernard Gallez, Cyril Corbet, Giulio G Muccioli, Olivier Feron, Patrice D Cani, Bénédicte F Jordan
2139 related Products with: Acetate: Friend or foe against breast tumour growth in the context of obesity?1100.00 ug
#33107119 2020/10/26 To Up
Study of common quantification methods of amide proton transfer magnetic resonance imaging for ischemic stroke detection.To assess the correlation and differences between common amide proton transfer (APT) quantification methods in the diagnosis of ischemic stroke.
Lee Sze Foo, James R Larkin, Brad A Sutherland, Kevin J Ray, Wun-She Yap, Yan Chai Hum, Khin Wee Lai, Hanani Abdul Manan, Nicola R Sibson, Yee Kai Tee
2950 related Products with: Study of common quantification methods of amide proton transfer magnetic resonance imaging for ischemic stroke detection.2x96 well plate100tests 5 G100tests100tests100tests100tests48 assays
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#33107092 2020/10/26 To Up
Monitoring Treatment Response and Disease Progression in Myeloma with Circulating Cell-Free DNA.Circulating cell free DNA (cfDNA) has the potential to capture spatial genetic heterogeneity in myeloma (MM) patients. We assessed whether cfDNA levels vary according to risk status defined by the 70 gene expression profile (GEP70). cfDNA levels in 77 patients was significantly higher in the GEP70 high risk (HR) group compared to the low risk (LR) group and correlated weakly with clinical markers including lactate dehydrogenase, β -microglobulin and ISS. Patients with high cfDNA levels were associated with a worse PFS (hazard ratio 6.4; 95% CI of ratio 1.9-22) and OS (hazard ratio 4.4; 95% CI of ratio 1.2-15.7). Circulating tumor DNA (ctDNA) was elevated in the HR group and ctDNA correlated strongly with GEP70 risk score (Spearman r=0.69, p=0.0027). cfDNA concentrations were significantly elevated between days 3-5 after chemotherapy before falling back to baseline levels. ctDNA in two patients showed a similar spike in levels between days 3-5 after chemotherapy with a concomitant increase in allele fraction of KRAS mutations. We assessed cfDNA levels in 25 patients with smoldering myeloma with serial samples and showed increased allele fraction of mutated KRAS at progression in cfDNA. Our study shows that cfDNA is a dynamic tool to capture genetic events in myeloma.
Shayu Deshpande, Ruslana G Tytarenko, Yan Wang, Eileen M Boyle, Cody Ashby, Carolina D Schinke, Sharmilan Thanendrarajan, Maurizio Zangari, Fenghuang Zhan, Faith E Davies, Gareth J Morgan, Frits van Rhee, Brian A Walker
1037 related Products with: Monitoring Treatment Response and Disease Progression in Myeloma with Circulating Cell-Free DNA.100ug Lyophilized
#33107091 2020/10/27 To Up
Increased S1P expression in osteoclasts enhances bone formation in an animal model of Paget's disease.Paget's disease (PD) is characterized by increased numbers of abnormal osteoclasts (OCLs) that drive exuberant bone formation, but the mechanisms responsible for the increased bone formation remain unclear. We previously reported that OCLs from 70% of PD patients express measles virus nucleocapsid protein (MVNP), and that transgenic mice with targeted expression of MVNP in OCLs (MVNP mice) develop bone lesions and abnormal OCLs characteristic of PD. In this report, we examined if OCL-derived sphingosine-1-phosphate (S1P) contributed to the abnormal bone formation in PD, since OCL-derived S1P can act as a coupling factor to increase normal bone formation via binding S1P-receptor-3 (S1PR3) on osteoblasts (OBs). We report that OCLs from MVNP mice and PD patients expressed high levels of sphingosine kinase-1 (SphK-1) compared with wild-type (WT) mouse and normal donor OCLs. SphK-1 production by MVNP-OCLs was interleukin-6 (IL-6)-dependent since OCLs from MVNP/IL-6 mice expressed lower levels of SphK-1. Immunohistochemistry of bone biopsies from a normal donor, a PD patient, WT and MVNP mice confirmed increased expression levels of SphK-1 in OCLs and S1PR3 in OBs of the PD patient and MVNP mice compared with normal donor and WT mice. Further, MVNP-OCLs cocultured with OBs from MVNP or WT mice increased OB-S1PR3 expression and enhanced expression of OB differentiation markers in MVNP-OBs precursors compared with WT-OBs, which was mediated by IL-6 and insulin-like growth factor 1 secreted by MVNP-OCLs. Finally, the addition of an S1PR3 antagonist (VPC23019) to WT or MVNP-OBs treated with WT and MVNP-OCL-conditioned media (CM) blocked enhanced OB differentiation of MVNP-OBs treated with MVNP-OCL-CM. In contrast, the addition of the SIPR3 agonist, VPC24191, to the cultures enhanced osterix and Col-1A expression in MVNP-OBs treated with MVNP-OCL-CM compared with WT-OBs treated with WT-OCL-CM. These results suggest that IL-6 produced by PD-OCLs increases S1P in OCLs and S1PR3 on OBs, to increase bone formation in PD.
Yuki Nagata, Kazuaki Miyagawa, Yasuhisa Ohata, Daniela N Petrusca, Gabriel M Pagnotti, Khalid S Mohammad, Theresa A Guise, Jolene J Windle, G David Roodman, Noriyoshi Kurihara
2025 related Products with: Increased S1P expression in osteoclasts enhances bone formation in an animal model of Paget's disease.900 tests 50 UG1-99 mg/ml/ea price x 225mg1-99 mg/ml/ea price x 250 ug 2 Pieces/Box
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