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#28802027   // To Up

Performance Evaluation of the Bioneer AccuPower® HIV-1 Quantitative RT-PCR kit: Comparison with the Roche COBAS® AmpliPrep/COBAS TaqMan® HIV-1 Test Ver.2.0 for Quantification of HIV-1 Viral Load in Indonesia.

Measurement of viral load in human immunodeficiency virus type 1 (HIV-1) infected patients is essential for the establishment of a therapeutic strategy. Several assays based on qPCR are available for the measurement of viral load; they differ in sample volume, technology applied, target gene, sensitivity and dynamic range. The Bioneer AccuPower® HIV-1 Quantitative RT-PCR is a novel commercial kit that has not been evaluated for its performance.
Agus Susanto Kosasih, Christine Sugiarto, Hubertus Hosti Hayuanta, Runingsih Juhaendi, Lyana Setiawan

1159 related Products with: Performance Evaluation of the Bioneer AccuPower® HIV-1 Quantitative RT-PCR kit: Comparison with the Roche COBAS® AmpliPrep/COBAS TaqMan® HIV-1 Test Ver.2.0 for Quantification of HIV-1 Viral Load in Indonesia.

1 G100tests100tests100tests250 mg100tests 100ul 1 G10mg10mg100Tests100tests

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#27374710   // To Up

Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR.

During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in Korea. We performed analytical and clinical validations of these kits.
Mi Na Kim, Young Jin Ko, Moon Woo Seong, Jae Seok Kim, Bo Moon Shin, Heungsup Sung

1302 related Products with: Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR.

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#26427158   // To Up

Comparison of the Microarray-Based Assay, the Real-Time PCR Assay, and the Bidirectional Sequencing Method for CYP2C19 Genotyping.

Cytochrome P450 2C19 (CYP2C19) is a clinically important enzyme that metabolizes a wide variety of drugs. Recently, some new genetic assays designed to identify the CYP2C19 genotype were introduced.
Hyun-Ki Kim, Hyun Jung Kang, Dae-Hyun Ko, Tae-Dong Jeong, Woochang Lee, Sail Chun, Won-Ki Min

1693 related Products with: Comparison of the Microarray-Based Assay, the Real-Time PCR Assay, and the Bidirectional Sequencing Method for CYP2C19 Genotyping.

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#25110642   2014/06/19 To Up

The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells.

The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells.
Youngdan Jeong, Wonkyung Yang, Hyunjung Ko, Miri Kim

1777 related Products with: The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells.

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#24963502   2014/05/13 To Up

Microbiological quality of fresh produce from open air markets and supermarkets in the Philippines.

This study is the first in the Philippines to conduct a comprehensive assessment of the prevalence of bacterial pathogens and somatic phages in retailed fresh produce used in salad preparation, namely, bell pepper, cabbage, carrot, lettuce, and tomato, using culture and molecular methods. Out of 300 samples from open air and supermarkets, 16.7% tested positive for thermotolerant Escherichia coli, 24.7% for Salmonella spp., and 47% for somatic phages. Results show that counts range from 0.30 to 4.03 log10 CFU/g for E. coli, 0.66 to ≥ 2.34 log10 MPN/g for Salmonella spp., and 1.30 to ≥ 3.00 log 10 PFU/g for somatic phages. Statistical analyses show that there was no significant difference in the microbial counts between open air and supermarkets (α = 0.05). TaqMan and AccuPower Plus DualStar real-time polymerase chain reaction (RT-PCR) was used to confirm the presence of these organisms. The relatively high prevalence of microorganisms observed in produce surveyed signifies reduction in shelf-life and a potential hazard to food safety. This information may benefit farmers, consumers, merchants, and policy makers for foodborne disease detection and prevention.
Pierangeli G Vital, Kris Genelyn B Dimasuay, Kenneth W Widmer, Windell L Rivera

2372 related Products with: Microbiological quality of fresh produce from open air markets and supermarkets in the Philippines.

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#24205489   2013/10/17 To Up

Macrolide resistance of Mycoplasma pneumoniae and its detection rate by real-time PCR in primary and tertiary care hospitals.

This study aimed to evaluate the prevalence of Mycoplasma pneumoniae in primary and tertiary care hospitals and its macrolide resistance rate.
Young Uh, Joo Hee Hong, Ki Jin Oh, Hyun Mi Cho, Soon Deok Park, Juwon Kim, Kap Jun Yoon

1046 related Products with: Macrolide resistance of Mycoplasma pneumoniae and its detection rate by real-time PCR in primary and tertiary care hospitals.

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#24157060   2013/09/28 To Up

Evaluation of a new real-time reverse transcription polymerase chain reaction assay for detection of norovirus in fecal specimens.

A new real-time reverse transcription polymerase chain reaction (RT-PCR) assay, the AccuPower Norovirus Real-time RT-PCR Kit, was evaluated in detection of human norovirus in stool specimens. Studies for detection limit, dynamic range, reproducibility, and cross-reactivity were performed. A total of 281 fecal specimens were tested using the AccuPower Norovirus Real-time RT-PCR Kit, and the results were compared with those obtained using another real-time RT-PCR system. Norovirus positivity and genotype were confirmed by direct sequencing. The lowest mean numbers of genome copies of GI and GII that could be detected by the assay were 12.3 and 5.6 RNA copies/reaction, respectively. The positive, negative, and overall percent agreements between the 2 real-time PCR assays were 99.0% (96/97), 95.1% (175/184), and 96.4% (271/281), respectively. The AccuPower Norovirus Real-time RT-PCR system showed good analytical and clinical performance and may be a useful diagnostic tool for norovirus infection.
Jungwon Hyun, Hyun Soo Kim, Han-Sung Kim, Kyu Man Lee

1627 related Products with: Evaluation of a new real-time reverse transcription polymerase chain reaction assay for detection of norovirus in fecal specimens.

25252525100 reactions250 Units25100 assays48 assays 96 assays100 assays

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