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#35749053   2022/06/24 To Up

Determination of association equilibrium constant from single molecule fluorescence localization microscopy.

Single molecule fluorescence localization microscopy provides molecular localization with a precision in the tens of nanometer range in the plane perpendicular to the light propagation. This opens the possibility to count molecules and correlate their locations, starting from a map of the actual positions in a single molecule super resolution image. Considering molecular pair correlation as an indication of interaction, and a way to discern them from free molecules, we describe a method to calculate thermodynamic equilibrium constants. In this work, we use as a test system two complementary homo-oligonucleotides, one strand marked with Cyanine 3.5 and the other with Alexa Fluor 647. Hybridization is controlled by the amount of each strand, temperature, and the ionic force, and measured in steady state emission. The same samples are examined in Stochastic Optical Reconstruction Microscopy (STORM) experiments with split-field simultaneous two-colour detection. The effect of multiblinking, labelling-detection efficiency, and determination of the critical distance for association are discussed. We consistently determine values in STORM coincident with those of the bulk experiment.
M Victoria Cappellari, Luis F Marcano-García, Sabrina Simoncelli, Pedro F Aramendía

1127 related Products with: Determination of association equilibrium constant from single molecule fluorescence localization microscopy.

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#35740840   2022/06/16 To Up

Dental Caries, Oral Health Behavior, and Living Conditions in 6-8-Year-Old Romanian School Children.

Dental caries still have a high prevalence in Romania. The aim of this paper is to determine the prevalence of caries in children (aged 6 to 8 years) correlated with individual-level predictors and socio-economic variables. A stratified, randomized nationally representative sample was established, taking into consideration the total number of preschool children and based on administrative units and residence. Self-assessment was performed by means of the Oral Health Questionnaire for Children (WHO). Examinations were conducted by 10 standardized examiners, with International Caries Detection and Assessment System (ICDAS) caries codes higher than 3 considered as dentinal caries, missing teeth as , and restorations as . DMFT and SiC indexes were calculated accordingly. The dataset for each outcome variable was analyzed by the Hurdle approach analyzed. The gender distribution was similar (47.22% male and 52.78% female), with 42.65% residing in rural areas. The mean DMFT value for the sample was 4.89 and SiC index 9.83. A negative association could be seen between DMFT and the father's level of education (β = -0.33, SE = 0.07, < 0.01) as well as the mother's education (β = -0.25, SE = 0.07, < 0.01). In conclusion, caries prevalence is very high in Romania as compared to the World Health Organization (WHO) recommendation for this age group in correlation with socio-economic factors and oral health behavior.
Ramona Dumitrescu, Ruxandra Sava-Rosianu, Daniela Jumanca, Octavia Balean, Lia-Raluca Damian, Guglielmo Giuseppe Campus, Laurentiu Maricutoiu, Vlad Tiberiu Alexa, Ruxandra Sfeatcu, Constantin Daguci, Mariana Postolache, Atena Galuscan

1092 related Products with: Dental Caries, Oral Health Behavior, and Living Conditions in 6-8-Year-Old Romanian School Children.

100 μg100 100 μg 5 G100ug Lyophilized

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#35724501   2022/06/15 To Up

Ruptured granulosa cell tumor of the ovary presenting with catastrophic intra-abdominal hemorrhage: A case report.

Adult granulosa cell tumor (GCT) is a rare stromal cell neoplasm that most often arises from the ovary. Presenting symptoms are related to external compression of adjacent structures (mass effect) or secretion of hormones such as estrogen. Patients most commonly present with irregular menstruation, postmenopausal bleeding, and abdominal pain. Prolonged estrogen exposure can contribute to endometrial adenocarcinoma development in untreated patients. The highly vascular nature of GCTs can lead to hemorrhagic rupture in rare cases.
Alexa D Smith, Mojdeh S Kappus, Wilfrido D Mojica, Martin J Caliendo, Connor Foote, Peter Muscarella

1694 related Products with: Ruptured granulosa cell tumor of the ovary presenting with catastrophic intra-abdominal hemorrhage: A case report.



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#35704798   // To Up

Molecular Characterization of Mesothelioma: Impact of Histologic Type and Site of Origin on Molecular Landscape.

Mesothelioma is an aggressive malignancy with heterogeneous outcomes that are partly driven by the differential efficacy of existing therapies across histologic types and sites of origin. Large-scale molecular analysis of mesothelioma and its subtypes has the potential to inform future therapeutic strategies.
Ibiayi Dagogo-Jack, Russell W Madison, Jochen K Lennerz, Kuei-Ting Chen, Julia F Hopkins, Alexa B Schrock, Lauren L Ritterhouse, Ashley Lester, Keith A Wharton, Mari Mino-Kenudson, Natalie Danziger, Yin P Hung, Douglas A Mata, Jeffrey S Ross

2022 related Products with: Molecular Characterization of Mesothelioma: Impact of Histologic Type and Site of Origin on Molecular Landscape.

5 G 6 ml Ready-to-use 2 ml Ready-to-use 2x5L1mg50 ug500 grams 2 ml Ready-to-use 20 ug 100 UG 25 ml Ready-to-use

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#35692737   2022/06/05 To Up

Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation.

A novel test strategy is proposed with dual-modality detection techniques for COVID-19 antibody detection. The full-length S protein of SARS-CoV-2 was chemically immobilized on a glass surface to capture anti-SARS-CoV-2 IgG in patient serum and was detected through either Electrochemical Impedance Spectroscopy (EIS) or fluorescence imaging with labeled secondary antibodies. Gold nanoparticles conjugated with protein G were used as the probe and the bound GNP-G was detected through EIS measurements. Anti-human-IgG conjugated with the fluorescent tag Alexa Fluor 488 was used as the probe for fluorescence imaging. Clinical SARS-CoV-2 IgG positive serum and negative controls were used to validate both modalities. For fluorescence-based detection, a high sensitivity was noticed with a quantification range of 0.01-0.1 A.U.C. and a LOD of 0.004 A.U.C. This study demonstrates the possibility of utilizing different measurement techniques in conjunction for improved COVID-19 serology testing.
Yuhao Ma, Daniel To, Jie Zeng, Lian C T Shoute, Meng Wu, Shawn Babiuk, Ran Zhuo, Carmen Charlton, Jamil N Kanji, Lorne Babiuk, Jie Chen

1987 related Products with: Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation.

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#35679544   // To Up

Culturally Safe Nursing Care for Black Sex Workers in the Greater Chicago Area, 2020‒2021.

Complex structural and social factors have created health inequities for Black sex workers. Black people, including those engaged in transactional sex, report leaning on spiritual beliefs to guide health-related decision-making, including whether to get the COVID-19 vaccine. Public health nurses can improve the health of Black sex workers through culturally safe care, which may include a community-stated vision of spiritual support. (. 2022;112(S3):S288-S291. https://doi.org/10.2105/AJPH.2022.306836).
Randi Beth Singer, Natasha Crooks, Amy K Johnson, Ariel U Smith, Linda Wesp, Rebecca Singer, Alexa Karczmar, Jahari Stamps, Bronwen Pardes, Crystal L Patil, Alicia K Matthews

2010 related Products with: Culturally Safe Nursing Care for Black Sex Workers in the Greater Chicago Area, 2020‒2021.

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#35679096   2022/06/09 To Up

Convenient Access to Expert-Reviewed Health Information via an Alexa Voice Assistant Skill for Patients With Multiple Myeloma: Development Study.

Patients with multiple myeloma (MM) have high information needs due to the complexity of the disease and variety of treatments. Digital voice assistants provide support in daily life and can be a convenient tool that even older patients can use to access health information. Voice assistants may therefore be useful in providing digital health services to meet the information needs of patients with MM.
Marc-Andrea Baertsch, Sarah Decker, Leona Probst, Stefan Joneleit, Hans Salwender, Franziska Frommann, Hartwig Buettner

1237 related Products with: Convenient Access to Expert-Reviewed Health Information via an Alexa Voice Assistant Skill for Patients With Multiple Myeloma: Development Study.

100ug/vial100ug Lyophilized100ug Lyophilized100ug Lyophilized1 module100ug Lyophilized100 μg100ug Lyophilized

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