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#34114295   2021/06/11 To Up

Liquid droplet formation and facile cytosolic translocation of IgG in the presence of attenuated cationic amphiphilic lytic peptides.

Fc region binding peptide conjugated with attenuated cationic amphiphilic lytic peptide L17E trimer [FcB(L17E)3] was designed for immunoglobulin G (IgG) delivery into cells. Particle-like liquid droplets were generated by mixing Alexa Fluor 488 labeled IgG (Alexa488-IgG) with FcB(L17E)3. Droplet contact with the cellular membrane led to spontaneous influx and distribution of Alexa488-IgG throughout cells in serum containing medium. Involvement of cellular machinery accompanied by actin polymerization and membrane ruffling was suggested for the translocation. Alexa488-IgG negative charges were crucial in liquid droplet formation with positively charged FcB(L17E)3. Binding of IgG to FcB(L17E)3 may not be necessary. Successful intracellular delivery of Alexa Fluor 594-labeled anti-nuclear pore complex antibody and anti-mCherry-nanobody tagged with supernegatively charged green fluorescence protein allowed binding to cellular targets in the presence of FcB(L17E)3.
Takahiro Iwata, Hisaaki Hirose, Kentarou Sakamoto, Yusuke Hirai, Jan Vincent V Arafiles, Misao Akishiba, Miki Imanishi, Shiroh Futaki

2355 related Products with: Liquid droplet formation and facile cytosolic translocation of IgG in the presence of attenuated cationic amphiphilic lytic peptides.

1mg20 20 100 96 tests100 0.1ml20 20 100 µg

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#34105487   // To Up

[The Role of Zyxin in Regulating Platelet Cytoskeleton Distribution].

To investigate the regulatory effect of zyxin on the distribution of platelet cytoskeleton.
Bin Cheng, Rong Yan, Su-Qin Zhang, Meng-Nan Yang, Ke-Sheng Dai

2006 related Products with: [The Role of Zyxin in Regulating Platelet Cytoskeleton Distribution].

100 UG2 Pieces/Box12 Pieces/Box

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#34098305   2021/06/04 To Up

Moralizing mental states: The role of trait self-control and control perceptions.

Which attributes of a person contribute to their tendency to moralize others' thoughts? Adopting an individual-difference approach to moral cognition, eight studies (N = 2,033) investigated how people's ability for self-control shapes their moral reactions to others' mental states. Specifically, Studies 1a-2b found positive predictive effects of trait self-control (TSC) on the moralization (e.g., blaming) of another person's fantasies about different immoral behaviors. While ruling out alternative explanations, they furthermore supported the mediating role of ascribing targets control over their mental states. Studies 3a-3b provided correlational evidence of the perceived ability to control one's own mental states as a mechanism in the relationship between TSC and ascriptions of control to others. Studies 4a-4b followed a causal-chain experimental approach: A manipulation of participants' self-perceived ability to control their emotions impacted their control ascriptions to others over their immoral mental states (Study 4a), and targets perceived as high (vs. low) in control over their immoral mental states elicited stronger moralizing reactions. Taken together, the present studies elucidate why people moralize others' purely mental states, even in the absence of overt behavior. More broadly, they advance our knowledge about the role of individual differences, particularly in self-control, in moral cognition.
Alexa Weiss, Matthias Forstmann, Pascal Burgmer

2847 related Products with: Moralizing mental states: The role of trait self-control and control perceptions.

100 Tests10.00 nmol 0.1 mg 500 100ug3 mg

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#34081875   2021/06/03 To Up

Exercise-nutrient interactions for improved postprandial glycemic control and insulin sensitivity.

Type 2 diabetes (T2D) is a rapidly growing yet largely preventable chronic disease. Exaggerated increases in blood glucose concentration following meals is a primary contributor to many long-term complications of the disease that decrease quality of life and reduce lifespan. Adverse health consequences also manifest years prior to the development of T2D due to underlying insulin resistance and exaggerated postprandial concentrations of the glucose-lowering hormone insulin. Postprandial hyperglycemic and hyperinsulinemic excursions can be improved by exercise, which contributes to the well-established benefits of physical activity for the prevention and treatment of T2D. The aim of this review is to describe the postprandial dysmetabolism that occurs in individuals at risk for and with T2D, and highlight how acute and chronic exercise can lower postprandial glucose and insulin excursions. In addition to describing the effects of traditional moderate-intensity continuous exercise on glycemic control, we highlight other forms of activity including low-intensity walking, high-intensity interval exercise, and resistance training. In an effort to improve knowledge translation and implementation of exercise for maximal glycemic benefits, we also describe how timing of exercise around meals and post-exercise nutrition can modify acute and chronic effects of exercise on glycemic control and insulin sensitivity. Novelty bullets • Exaggerated postprandial blood glucose and insulin excursions are associated with disease risk • Both a single session and repeated sessions of exercise improve postprandial glycemic control in individuals with and without T2D • The glycemic benefits of exercise can be enhanced by considering the timing and macronutrient composition of meals around exercise.
Jenna B Gillen, Stephanie Estafanos, Alexa Govette

1190 related Products with: Exercise-nutrient interactions for improved postprandial glycemic control and insulin sensitivity.

100ug Lyophilized50 TESTS100-150ul100ul5 mg2 mg100ug2 mL100ug 0.1 mg 100ug

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#34081019   2021/06/03 To Up

Chatbots to Support People With Dementia and Their Caregivers: Systematic Review of Functions and Quality.

Over the past decade, there has been an increase in the use of information technologies to educate and support people with dementia and their family caregivers. At the same time, chatbot technologies have become increasingly popular for use by the public and have been identified as having benefits for health care delivery. However, little is known about how chatbot technologies may benefit people with dementia and their caregivers.
Nicole Ruggiano, Ellen L Brown, Lisa Roberts, C Victoria Framil Suarez, Yan Luo, Zhichao Hao, Vagelis Hristidis

2417 related Products with: Chatbots to Support People With Dementia and Their Caregivers: Systematic Review of Functions and Quality.

500 MG1000 TESTS/0.65ml25 mg10 mg100ug100ul50 mg25 mg96T100ug1000 10 mg

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#34080035   2021/06/03 To Up

A 15-min non-competitive homogeneous assay for microcystin and nodularin based on time-resolved Förster resonance energy transfer (TR-FRET).

Simple and rapid methods are required for screening and analysis of water samples to detect cyanobacterial cyclic peptide hepatotoxins: microcystin/nodularin. Previously, we reported a highly sensitive non-competitive heterogeneous assay for microcystin/nodularin utilizing a generic anti-immunocomplex (anti-IC) single-chain fragment of antibody variable domains (scFv) isolated from a synthetic antibody library together with a generic adda ((2S,3S,4E,6E,8S,9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid)-specific monoclonal antibody (Mab) recognizing the common adda part of the microcystin/nodularin. Using the same antibody pair, here we report a homogeneous non-competitive assay for microcystin/nodularin based on TR-FRET (time-resolved Förster resonance energy transfer) measurement. The anti-IC scFv labeled with Alexa Fluor 680 and the Mab labeled with europium enabled the FRET process to occur in the presence of microcystin/nodularin. The TR-FRET signal is proportional to the toxin concentration in the sample. The rapid (15 min) homogeneous assay without requiring any washing step detected all the tested nine toxin variants (microcystin-LR, -dmLR, -RR, -dmRR, -YR, -LY, -LF -LW, and nodularin-R). Very good signal to blank ratio (~13) was achieved using microcystin-LR and the sample detection limit (blank+3SD of blank) for microcystin-LR was ~0.3 μg/L (~0.08 μg/L in 80-μL reaction well). The practical application of the TR-FRET assay was demonstrated with water samples spiked with microcystin-LR as well as with environmental water. The average recoveries of microcystin-LR from spiked water ranged from 65 to 123%. Good correlation (r = 0.73 to 0.99) with other methods (liquid chromatography-mass spectrometry and previously reported heterogeneous assay) was found when environmental samples were analyzed. The developed wash-free assay has the potential to play as a quick screening tool to detect microcystin/nodularin from water below the World Health Organization's guideline limit (1 μg/L of microcystin-LR).
Sultana Akter, Urpo Lamminmäki

1527 related Products with: A 15-min non-competitive homogeneous assay for microcystin and nodularin based on time-resolved Förster resonance energy transfer (TR-FRET).

100 tests100 assays10 plates100 assays1 plate96 Tests1,000 tests50 assays

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#34077268   2021/06/02 To Up

Clinicopathologic Features and Response to Therapy of Fusion-Driven Lung Cancers: The eNRGy1 Global Multicenter Registry.

Although fusions are oncogenic drivers across multiple tumor types including lung cancers, these are difficult to study because of their rarity. The global eNRGy1 registry was thus established to characterize fusion-positive lung cancers in the largest and most diverse series to date.
Alexander Drilon, Michael Duruisseaux, Ji-Youn Han, Masaoki Ito, Christina Falcon, Soo-Ryum Yang, Yonina R Murciano-Goroff, Haiquan Chen, Morihito Okada, Miguel Angel Molina, Marie Wislez, Philippe Brun, Clarisse Dupont, Eva Branden, Giulio Rossi, Alexa Schrock, Siraj Ali, Valérie Gounant, Fanny Magne, Torsten Gerriet Blum, Alison M Schram, Isabelle Monnet, Jin-Yuan Shih, Joshua Sabari, Maurice Pérol, Viola W Zhu, Misako Nagasaka, Robert Doebele, D Ross Camidge, Maria Arcila, Sai-Hong Ignatius Ou, Denis Moro-Sibilot, Rafael Rosell, Lucia Anna Muscarella, Stephen V Liu, Jacques Cadranel

2934 related Products with: Clinicopathologic Features and Response to Therapy of Fusion-Driven Lung Cancers: The eNRGy1 Global Multicenter Registry.

1 mg 1 G200ul96 Tests n50 ug 1 module

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