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#34130235   2021/06/12 To Up

Advances in the study of GPCRs by F NMR.

Crystallography and cryo-electron microscopy have advanced atomic resolution perspectives of inactive and active states of G protein-coupled receptors (GPCRs), alone and in complex with G proteins or arrestin. F NMR can play a role in ascertaining activation mechanisms and understanding the complete energy landscape associated with signal transduction. Fluorinated reporters are introduced biosynthetically via fluorinated amino acid analogs or chemically, via thiol-specific fluorinated reporters. The chemical shift sensitivity of these reporters makes it possible to discern details of conformational ensembles. In addition to spectroscopic details, paramagnetic species can be incorporated through orthogonal techniques to obtain distance information on fluorinated reporters, while T-and T-based relaxation experiments provide details on exchange kinetics in addition to fluctuations within a given state.
Louis-Philippe Picard, Robert Scott Prosser

2497 related Products with: Advances in the study of GPCRs by F NMR.

0.1 mg0.2 mg500 ml1 Set100ug Lyophilized1 Set

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#34130198   2021/06/06 To Up

Amino- and carboxyl-terminal ends of the bovine parainfluenza virus type 3 matrix protein are important for virion and virus-like particle release.

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Haruna Ueda, Nagisa Yamakawa, Kaoru Takeuchi

1294 related Products with: Amino- and carboxyl-terminal ends of the bovine parainfluenza virus type 3 matrix protein are important for virion and virus-like particle release.

50 10 10 1 mg50 10 1 mg1 mg50 10001 mL500

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#34130112   2021/06/02 To Up

Glutamine addiction in virus-infected mammalian cells: A target of the innate immune system?

Control of core cell metabolism is a key aspect of the evolutionary conflict between viruses and the host's defence mechanisms. From their side, the invading viruses press the accelerator on their host cell's glycolysis, fatty acid, and glutaminolytic metabolic processes among others. It is also well established that activation of innate immune system responses modulates facets of metabolism such as that of polyamine, cholesterol, tryptophan and many more. But what about glutamine, a proteogenic amino acid that is a crucial nutrient for multiple cellular biosynthetic processes? Although mammalian cells can normally synthesize glutamine de novo, it has been noted that infections with genetically and phylogenetically diverse viruses are followed by the acquisition of a dependency on supplies of exogenous glutamine i.e. "glutamine addiction". Here we present our novel hypothesis that glutamine metabolism is also a target of the innate immune system, possibly through the action of interferons, as part of the evolutionary conserved antiviral metabolic reprogramming.
C Koufaris, V Nicolaidou

1204 related Products with: Glutamine addiction in virus-infected mammalian cells: A target of the innate immune system?

1mg100 µg100 µg1 mg1 mg1 mg0.1ml96 assays250.25 mg

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#34130101   2021/06/12 To Up

Oxidation induced by dielectric barrier discharge (DBD) plasma treatment reduces IgG/IgE binding capacity and improves the functionality of glycinin.

The effect of dielectric barrier discharge (DBD) plasma treatment times from 2 to 5 min at 40 kV on IgG/IgE binding capacity and functionality of soybean glycinin was examined. A substantial reduction in the binding capacity (91.64% for IgG and 81.49% for IgE) was obtained after 5 min of plasma treatment, as determined by western-blot and ELISA analyses. Further studies demonstrated that the elimination of antigenicity and allergenicity of glycinin was directly related to plasma-induced structural changes on two aspects. A conformational alteration caused by oxidation of peptide bond amino groups, accompanied with an oxidation of Trp, Tyr, and Phe amino acid residues, which was confirmed by surface hydrophobicity, multi-spectroscopic analysis, and amino acid analysis. The cleavage of polypeptide chains inevitably partially diminished the linear epitopes, resulting in a primary decline in IgG/IgE binding capacity. Additionally, an increase in the solubility from 10.78 ± 0.35 to 65.96 ± 1.86% and significant increase in the emulsifying ability from 21.08 ± 2.64 to 160.29 ± 4.12 m/g were observed after treatment of the plasma for 2 min. The present results confirm the potential use of DBD for the production of hypoallergenic soy protein-based products and improving their technical functions such as solubility and emulsifying ability.
Zhi-Wei Liu, Ying-Xue Zhou, Feng Wang, Yi-Cheng Tan, Jun-Hu Cheng, Alaa El-Din Bekhit, Rana Muhammad Aadil, Xiu-Bin Liu

2863 related Products with: Oxidation induced by dielectric barrier discharge (DBD) plasma treatment reduces IgG/IgE binding capacity and improves the functionality of glycinin.

96T1000 TESTS/0.65ml96T96T0.1 ml1 mg50 ug

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#34130082   2021/05/31 To Up

Changes in molecular structure of extracellular polymeric substances (EPS) with temperature in relation to sludge macro-physical properties.

Extracellular polymeric substances (EPS) are important components of activated sludge, whose content and composition have important effects on the macro-physical properties of sludge. In this study, the response of EPS in sludge to temperature (-40-200 °C) was systematically investigated using XAD resin fractionation, variable-temperature infrared spectra (VTIS) and two-dimensional correlation spectroscopy (2D-COS). The relationships between the molecular structure of EPS and the macro-physical properties (rheological property and dewatering performance) of waste activated sludge (WAS) at varying temperature were also established. During the freezing treatment, the solubilization of biopolymers and destruction of the hydrophilic functional groups (hydroxy, amino and carboxyl) resulted in the production of small organic matters, which enhanced EPS hydrophobicity and reduced electrostatic repulsion of sludge, and subsequent dewaterability improvement. For the hydrothermal treatment, the EPS transformation showed a two-stages reaction including stage I (70-120 °C) and stage II (>120 °C). Stage I (70-120 °C), a plenty of hydrophilic functional groups (hydroxy, amino and carboxyl) in EPS were exposed via the solubilization of biopolymers, which enhanced electrostatic repulsion of sludge and EPS hydrophilicity, and subsequence in deterioration of sludge dewaterability and fluidity. However, at stage II (>120 °C), the high temperature caused hydrolyzation of macromolecular organic matters in completely, in which the secondary structure of the protein was destroyed, causing the peptide chain to unfold. In addition, the reduction of α-helix and β-sheet content and intensified Maillard reaction decreased electrostatic repulsion of sludge, thus resulted in the improvement of sludge dewaterability and fluidity. This study enriched the theoretical basis of the optimal control of sludge treatment based on temperature regulation.
Sainan Peng, Aibin Hu, Jing Ai, Weijun Zhang, Dongsheng Wang

2892 related Products with: Changes in molecular structure of extracellular polymeric substances (EPS) with temperature in relation to sludge macro-physical properties.

96 tests96 wells (1 kit)1 mg1 kit 1 G1mg500 gm.100 μg

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#34130013   2021/05/21 To Up

Development and validation of a novel sepsis biomarker based on amino acid profiling.

Sepsis is a potentially fatal condition influenced by pathogens and host factors. Current sepsis biomarkers such as white blood cell count and C-reactive protein and procalcitonin levels show unsatisfactory performance in terms of diagnostic sensitivity and specificity in clinical practice. Thus, we developed and validated a new sepsis biomarker based on amino acid profiling.
Sunyoung Ahn, Su Hwan Lee, Kyung Soo Chung, Nam Su Ku, Young-Min Hyun, Sail Chun, Moo Suk Park, Sang-Guk Lee

2440 related Products with: Development and validation of a novel sepsis biomarker based on amino acid profiling.

5 mg 1 G5 g 500 G10 mg 500 G50 mg5 g50 mg10 mg50 mg1 g

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#34130010   2021/06/10 To Up

The nitrosamine contamination of drugs, part 3: Quantification of 4-Methyl-1-nitrosopiperazine in rifampicin capsules by LC-MS/HRMS.

Upon emergence of nitrosamines in various drugs, e.g in valsartan, metformin and ranitidine, 4-methyl-1-nitrosopiperazine (MeNP) was found in rifampicin in August 2020. Rifampicin is used, amongst others, for post-exposure prophylaxis of leprosy. The occurrence of MeNP can be explained by the synthesis, because 1-amino-4-methylpiperazine is concomitantly used with the organic oxidizing reagent isoamyl nitrite. According to a method reported by the FDA, the quantification of MeNP in rifampicin capsules was performed by LC-MS/HRMS. A significant contamination with MeNP was found in all samples, ranging from 0.7 to 5.1 ppm and exceeding the acceptable intake limit proposed by the FDA up to 32-fold. However, the severity of a possible leprosy infection outweighs the risks, which are concomitant with the intake of a single dose of rifampicin for post-exposure prophylaxis. Nevertheless, the extent of contamination is alarming, and countermeasures are needed to minimize public health risks. The presence of nitrosamines in rifampicin illustrates the need for better strategies in impurity profiling and compendial testing once again.
Jonas Wohlfart, Oliver Scherf-Clavel, Martina Kinzig, Fritz Sörgel, Ulrike Holzgrabe

1257 related Products with: The nitrosamine contamination of drugs, part 3: Quantification of 4-Methyl-1-nitrosopiperazine in rifampicin capsules by LC-MS/HRMS.

10 mg50 mg10 mg0.5 mg 1 G50ug10 mg10 mg

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