Search results for: Amplite™ Colorimetric Aldehyde Quantitation Kit
#29734927 2018/05/07 To Up
Evaluation of potential biomarkers of exposure to crystalline silica: A case study in an insulator manufacturer.
The purpose of this study was to examine the potential determinants of serum neopterin, malondialdehyde (MDA), and erythrocyte glutathione (GSH) as potential markers of oxidative stress, resulting in cellular immune response to inhaled silica particles. This descriptive analytical study was conducted on two groups of exposed workers ( n = 55) and unexposed office workers ( n = 38) of an insulator manufacturing plant. The sampling of airborne silica in the breathing zone of participants was done on the basis of the National Institute for Occupational Safety and Health Method 7601. The blood samples were analyzed by high performance liquid chromatography to determine the level of serum neopterin. A ZellBio GmbH assay kit was used for the quantitative assays of GSH and MDA on the basis of the colorimetric assay. The results of this study show that the measurements of serum neopterin, MDA, and GSH can be considered as potential biological markers of silica exposure for undertaking further comprehensive studies in this area.Hamzeh Mohammadi, Somayeh Farhang Dehghan, Alireza Tahamtan, Farideh Golbabaei
2021 related Products with: Evaluation of potential biomarkers of exposure to crystalline silica: A case study in an insulator manufacturer.
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#25344211 2014/10/25 To Up
ALDH1A1 mediates resistance of diffuse large B cell lymphoma to the CHOP regimen.
Although there have been substantial advances in our knowledge of the resistance of diffuse large B cell lymphoma (DLBCL) to chemotherapy, there are few efficient treatment strategies for recurrent/refractory DLBCL. The aim of this study was to investigate the role of aldehyde dehydrogenase (ALDH) 1A1 in the resistance of diffuse large B cell lymphoma to the chemotherapeutic mixture consisting of cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP). The involvement of ALDH1A1 in DLBCL was elucidated by knockdown and pharmacologic inhibition; Cell Counting Kit-8 (CCK-8) and clone formation assays were used to determine its role in CHOP sensitivity and clone formation ability. Caspase colorimetric assay was used to measure the extent of apoptosis. Western blot analysis was used to measure signal transducer and activator of transcription 3 (STAT3)/nuclear factor kappa B (NF-κB) signaling proteins, and quantitative real-time PCR (RT-PCR) was used to measure the differential expression of ALDH1A1 of DLBCL patients and healthy donors. ALDH1A1 showed a 5.64-fold higher expression in malignant B cells than in normal B cells. Diethylaminobenzaldehyde (DEAB) decreased the half maximal inhibitory concentration (IC50) of the CHOP regimen in Farage cells from 344.78 ± 65.75 to 183.88 ± 49.75 ng/ml (P = 0.004). Both knockdown and inhibition of ALDH1A1 reduced clonogenicity, increased caspase-3/caspase-9 activity, and attenuated the phosphorylation status of STAT3/NF-κB. The prognosis of patients with a high level of ALDH1A1 expression was poor compared with that of patients with low levels of expression (P = 0.044). ALDH1A1 is a new mediator for resistance of DLBCL to CHOP; it is a predictor of clinical prognosis and may serve as a potential target to improve chemotherapy responsiveness of human DLBCL.Ying-Hui Song, Mei-Zuo Zhong, Ping-Ping Gan, Ping-Yong Yi, You-Hong Tang, Yi-Ping Liu, Jin-Qiong Jiang, Li Li
1824 related Products with: ALDH1A1 mediates resistance of diffuse large B cell lymphoma to the CHOP regimen.
100 extractions430 tests10mlRelated Pathways
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