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Search results for: Androsta-1,4,6-triene-3,17-dione C19H22O2 CAS: 633-35-2

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#34343787   2021/07/31 To Up

Volition in prospective Memory: Evidence against differences between free and fixed target events.

Volition is the extent to which actions are generated as a result of internal states in contrast to responses to external conditions or dictated by external events. Delayed intentions about future action are stored in prospective memory until the intended action has to be formed at a later point in time. It is unknown how voluntary choice affects prospective memory. We compared the difference between freely chosen and fixed targets on the reaction times and task performance in the ongoing task and for the target stimuli in a prospective memory task. The task performance and the reaction time was modelled using a Bayesian hierarchical drift-diffusion model. The analysis showed no differences between self-chosen and fixed prospective memory cues on task responses. The result suggests that volition in choosing the cue to act upon or given a fixed cue does not make a difference for prospective memory task performance.
Mikkel C Vinding, Jonas Kristoffer Lindeløv, Yahui Xiao, Raymond C K Chan, Thomas Alrik Sørensen

1228 related Products with: Volition in prospective Memory: Evidence against differences between free and fixed target events.

50ul0.1ml1 set0.1ml1 mg0.1ml (1mg/ml)1 ml0.1ml (1mg/ml)1mg0.1ml0.1ml (1mg/ml)1mg

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#34343636   2021/07/31 To Up

AKT1/FOXP3 axis-mediated expression of CerS6 promotes p53 mutant pancreatic tumorigenesis.

Ceramide synthases (CerSs) catalyze the formation of ceramides from sphingoid bases and acyl-CoA substrates. Increasing evidence suggests that cancer cells generally exhibit altered sphingolipid metabolism in the tumorigenesis of multiple cancers. However, there is no evidence that CerSs are associated with pancreatic ductal carcinoma (PDAC). In the present study, we examined CerS expression in clinical tissue and conducted data mining to investigate the clinical significance of CerSs in the TCGA-PAAD database. We found that high CerS6 expression positively correlated with progression and predicted worse prognosis in PDAC patients, establishing CerS6 as a potential biomarker for PDAC. Furthermore, CerS6 promoted cell proliferation, colony formation and invasion by producing C16-ceramide and was required for tumor formation. Mechanistically, AKT1 interacted with and phosphorylated FOXP3 at S418, which decreased the binding of FOXP3 to the CERS6 promoter and in turn induced CerS6 expression by reconstituting an activated state on the CERS6 promoter. The AKT1/FOXP3 axis mediated the CerS6 expression and promoted p53 mutant pancreatic tumorigenesis by producing excessive C16-ceramide, which induced the accumulation of mutant p53. Thus, our study explores the relationship between PI3K/AKT signaling and sphingolipid metabolism, revealing an oncogenic role for CerS6, which may represent a potential target for PDAC treatment.
Dachuan Qi, Xuwei Song, Chunhua Xue, Wenyan Yao, Penghui Shen, Hua Yu, Zhiqi Zhang

1646 related Products with: AKT1/FOXP3 axis-mediated expression of CerS6 promotes p53 mutant pancreatic tumorigenesis.

25UG1 Set1mg1 Set50 ug250ul0.5mg

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#34343279   2021/08/03 To Up

Efficient One-Step Separation of Five Flavonoids from the Crude Extract of the Waste Pomace of Sea Buckthorn Berries through Counter-Current Chromatography.

The pomace of sea buckthorn berries is usually discarded when transforming into nonalcoholic or alcoholic beverages, jellies, jams, juices, candies and dairy products. Here, we established a promising approach for one step separation of five flavonoids from the waste pomace of sea buckthorn berries through counter-current chromatography. The crude extract of waste pomace of sea buckthorn berries after juicing was injected into counter-current chromatography with hexane/ethyl acetate/ethanol/water (v/v/v/v, 5:7:5:7) as the solvent system. As a result, five flavonoids, including quercetin, laricitrin, isorhamnetin-7-O-rhamnoside, kaempferol and isorhamnetin, were obtained in a single step separation. Our finding showed that ethanol is a good substitute for methanol to regulate the partition coefficient in hexane/ethyl acetate/ methanol/water system. This study provided a significant measure to utilize the waste pomace of Sea buckthorn berries.
Tao Chen, Shuo Wang, Hongmei Li, Cheng Shen, Shuping Yan, Yangfei Wei, Zhibo Song, Peipei Li, Yulin Li

2903 related Products with: Efficient One-Step Separation of Five Flavonoids from the Crude Extract of the Waste Pomace of Sea Buckthorn Berries through Counter-Current Chromatography.

5 G11mg1 ml12000 IU100.00 ul500 Units

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#34343211   2021/08/03 To Up

Efficient Dicer processing of virus-derived double-stranded RNAs and its modulation by RIG-I-like receptor LGP2.

The interferon-regulated antiviral responses are essential for the induction of both innate and adaptive immunity in mammals. Production of virus-derived small-interfering RNAs (vsiRNAs) to restrict virus infection by RNA interference (RNAi) is a recently identified mammalian immune response to several RNA viruses, which cause important human diseases such as influenza and Zika virus. However, little is known about Dicer processing of viral double-stranded RNA replicative intermediates (dsRNA-vRIs) in mammalian somatic cells. Here we show that infected somatic cells produced more influenza vsiRNAs than cellular microRNAs when both were produced by human Dicer expressed de novo, indicating that dsRNA-vRIs are not poor Dicer substrates as previously proposed according to in vitro Dicer processing of synthetic long dsRNA. We report the first evidence both for canonical vsiRNA production during wild-type Nodamura virus infection and direct vsiRNA sequestration by its RNAi suppressor protein B2 in two strains of suckling mice. Moreover, Sindbis virus (SINV) accumulation in vivo was decreased by prior production of SINV-targeting vsiRNAs triggered by infection and increased by heterologous expression of B2 in cis from SINV genome, indicating an antiviral function for the induced RNAi response. These findings reveal that unlike artificial long dsRNA, dsRNA-vRIs made during authentic infection of mature somatic cells are efficiently processed by Dicer into vsiRNAs to direct antiviral RNAi. Interestingly, Dicer processing of dsRNA-vRIs into vsiRNAs was inhibited by LGP2 (laboratory of genetics and physiology 2), which was encoded by an interferon-stimulated gene (ISG) shown recently to inhibit Dicer processing of artificial long dsRNA in cell culture. Our work thus further suggests negative modulation of antiviral RNAi by a known ISG from the interferon response.
Yuqiang Zhang, Yan Xu, Yunpeng Dai, Zhe Li, Jiaxing Wang, Zhi Ye, Yanxin Ren, Hua Wang, Wan-Xiang Li, Jinfeng Lu, Shou-Wei Ding, Yang Li

1777 related Products with: Efficient Dicer processing of virus-derived double-stranded RNAs and its modulation by RIG-I-like receptor LGP2.

200 100.00 ul200ul5mg100ug100ug100ul50 ug 1000 200ug100ug

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#34343062   // To Up

sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent chimney in the Southwest Indian Ridge.

A hyperthermophilic, strictly anaerobic archaeon, designated strain SY113, was isolated from a deep-sea hydrothermal vent chimney on the Southwest Indian Ridge at a water depth of 2770 m. Enrichment and isolation of strain SY113 were performed at 85 °C at 0.1 MPa. Cells of strain SY113 were irregular motile cocci with peritrichous flagella and generally 0.8-2.4 µm in diameter. Growth was observed at temperatures between 50 and 90 °C (optimum at 85 °C) and under hydrostatic pressures of 0.1-60 MPa (optimum, 27 MPa). Cells of SY113 grew at pH 4.0-9.0 (optimum, pH 5.5) and a NaCl concentration of 0.5-5.5 % (w/v; optimum concentration, 3.0 % NaCl). Strain SY113 was an anaerobic chemoorganoheterotroph and grew on complex proteinaceous substrates such as yeast extract and tryptone, as well as on maltose and starch. Elemental sulphur stimulated growth, but not obligatory for its growth. The G+C content of the genomic DNA was 55.0 mol%. Phylogenetic analysis of the 16S rRNA sequence of strain SY113 showed that the novel isolate belonged to the genus . On the basis of physiological characteristics, average nucleotide identity values and DNA-DNA hybridization results, we propose a novel species, named sp. nov. The type strain is SY113 (=MCCC 1K04190=JCM 39083).
Xue-Gong Li, Hong-Zhi Tang, Wei-Jia Zhang, Xiao-Qing Qi, Zhi-Guo Qu, Jun Xu, Long-Fei Wu

2663 related Products with: sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent chimney in the Southwest Indian Ridge.

50 ul100.00 ug100 ul100 ul50 ul

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#34342978   2021/08/03 To Up

α-Active Pyrylium Salt 2,4,5-Triphenylpyrylium for Improved Mass Spectrometry-Based Detection of Peptides.

Pyrylium salts are considered efficient chemical tags for amino groups. However, the apparent steric selectivity of pyrylium salts limits their application in the field of chemical labeling, especially during the labeling of sterically hindered compounds like amino acids, peptides, and proteins. Herein, we have investigated the effects of the α-substitution of pyrylium salts on their reactivity. We have also investigated the mechanism of nucleophilic reactions with pyrylium salts and further proposed that the reactivity of pyrylium salts mainly depends on the position and type of their substituents. A series of pyrylium salts were synthesized, and a highly active α-monosubstituted pyrylium salt, 2,4,5-triphenylpyrylium, was developed for efficient chemical labeling. All of the 15 amino acids studied were efficiently labeled under optimized reaction conditions. The 2,4,5-triphenylpyrylium salt was highly efficient in comparison to the previously reported 2,4,6-triphenylpyrylium salt developed for lysine-specific modifications. Furthermore, we successfully used 2,4,5-triphenylpyrylium salt for the hydrophobic labeling of peptides and protein hydrolysates. The most striking observation was that the ionization efficiency of short-chain multilabeled peptides in mixed samples, after derivatization, increased by up to 60 times. The increase in ionization efficiency gradually decreased with increasing peptide chain length. During the "soft" collision-induced dissociation (CID) process, the peptide was tagged at the N-terminus with 2,4,5-triphenylpyrylium, producing abundant a-type ions and b-type ions (Δ = 28), which eases the peptide resequencing process and assists in cracking the peptide codes. Moreover, 2,4,5-triphenylpyrylium has been utilized for the proteomic analysis of HeLa cell digests. In addition, 215 additional proteins were identified in the labeled products and the coverage of most proteins was improved.
Shuai Huang, Sivakumar Palanisamy, Xi Yu, Yi Wang, Dan Liu, Weitao Gong, Xiaozhe Zhang

1479 related Products with: α-Active Pyrylium Salt 2,4,5-Triphenylpyrylium for Improved Mass Spectrometry-Based Detection of Peptides.

2x96 well plate1.0 mg500 tests

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#34342969   2021/08/03 To Up

Heterojunction of Vertically Arrayed MoS Nanosheet/N-Doped Reduced Graphene Oxide Enabling a Nanozyme for Sensitive Biomolecule Monitoring.

Enzymes are still indispensable for bio-assaying methods in biomolecule detection by far. The unsatisfied long-term instability, high cost, and susceptibility to the physical environment of natural enzymes are obvious weak points. Here, we developed peroxidase-like heterostructured nanozyme, vertically arraying molybdenum disulfide nanosheets on a substrate layer of nitrogen-doped reduced graphene oxide (MoS/N-rGO), with a well-pleasing stability that is characterized by the retained enzymatic activity and maintained structure after 2 years of casual storage at ambient temperatures or 80 cycles of catalytic reaction. The catalytic kinetics of the as-prepared heterostructured nanozyme was superior to some reported nanozymes and even horse radish peroxidase, which was demonstrated due to the defect-rich MoS with Mo and S vacancies and nitrogen-doped rGO experimentally and theoretically. The vertically heterostructured nanozyme exhibited adequate analytical performance in sensitive and quantitative detection of glucose and glutathione (GSH), with a large dynamic sensing range and extremely low limit of detection (0.02 and 0.12 μM (3σ/slope) for glucose and GSH, respectively). We hope this inspired artificial nanozyme will contribute to the future development in sensitive detection of other biomolecules in physiological conditions.
Longwei Wang, Bo Li, Zhen You, Aizhu Wang, Xuanyu Chen, Gaojing Song, Ling Yang, Dan Chen, Xin Yu, Jing Liu, Chunying Chen

1319 related Products with: Heterojunction of Vertically Arrayed MoS Nanosheet/N-Doped Reduced Graphene Oxide Enabling a Nanozyme for Sensitive Biomolecule Monitoring.

96 tests 100ul1 mg100 mg25 gFor 2 miRNA probes, each 1 G25 mg1mg

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#34342965   2021/08/03 To Up

Interfacial Intermixing and Its Impact on the Energy Band Structure in Interband Cascade Infrared Photodetectors.

Multiple-stage interband cascade infrared photodetector (ICIP) is a new class of semiconductor infrared photodetector that exhibits improved device performance in terms of responsivity and detectivity. The design of the device structure and the electronic structure on superlattices and quantum wells assume abrupt interfaces. However, the emergence of possible interface segregation and atom exchange can only be determined experimentally, impacting the device performance. In this work, the interface atom intermixing and their effects on the energy band structure in a molecular beam epitaxy grown ICIP are studied. Scanning transmission electron microscopy (STEM) reveals atom diffusion and intermixing between the constituent layers of the cascade structure, causing a shift in the quantum state energy levels of the layers and the consequent misalignment of the cascade structures. Combining the STEM observation with high-resolution X-ray diffraction, the alloy composition profiles of the layers are determined. Using the "real" graded composition profiles, the effective band gap of the superlattice absorber and the energy levels of the relaxation region and the tunneling region are recalculated showing a cutoff wavelength of the superlattice absorber 4.93 μm, which is 0.78 μm smaller than that calculated using the nominal step composition profile. However, its agreement is greatly improved with the measured cutoff wavelength of 5.03 μm. The energy level of the narrowest quantum well in the relaxation region is 0.091 eV higher than the conduction miniband of the absorber, which is also consistent with the experiments that the pho-response exits a "turn on" voltage of 0.1 V. The results reported here will help optimize the energy structure design of future ICIP with improved device performance.
Xuliang Chai, Roger Guzman, Yi Zhou, Zhicheng Xu, Zhaoming Liang, Yihong Zhu, Wu Zhou, Jianxin Chen

1748 related Products with: Interfacial Intermixing and Its Impact on the Energy Band Structure in Interband Cascade Infrared Photodetectors.

1 kit11 mg1 mg1 mg96 tests

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#34342820   2021/08/03 To Up

Exosomes from antler stem cells alleviate mesenchymal stem cell senescence and osteoarthritis.


Jinghui Lei, Xiaoyu Jiang, Wei Li, Jie Ren, Datao Wang, Zhejun Ji, Zeming Wu, Fang Cheng, Yusheng Cai, Zheng-Rong Yu, Juan Carlos Izpisua Belmonte, Chunyi Li, Guang-Hui Liu, Weiqi Zhang, Jing Qu, Si Wang

2533 related Products with: Exosomes from antler stem cells alleviate mesenchymal stem cell senescence and osteoarthritis.

5 x 10A5 cells/vial1 x 10^6 cells/vial124 wells1 mg24 wells10 ug24 wells33 Modulators2ug100 ug

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