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Search results for: Androstenedione-19 Antibody

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#33984827   2021/04/26 To Up

Determination of free sulfhydryl contents for proteins including monoclonal antibodies by use of SoloVPE.

Free sulfhydryls are important properties of protein products including monoclonal antibodies (mAbs). Here, a new technology, variable pathlength extension (SoloVPE), is employed to quantify the amount of free sulfhydryl in monoclonal antibodies (mAbs) using the well-known Ellman reagent. Briefly, the unbound thiols (free sulfhydryls) of proteins including mAbs react with Ellman reagent to produce a 2-nitro-5-thiobenzoate (TNB) which is detected at visible wavelength of 412 nm and quantified. The method does not require dilution of antibody samples, is simple, reproducible and takes less than one hour to complete. Values obtained by the new method are compared to literature values from traditional UV or fluorescence methods with agreements. Qualification and trending data over two years of method utilization in our labs support that assay variability is minimal with an intermediate precision of relative standard deviation (RSD) ≤ 10 % and a limit of quantification (LOQ) of 0.1 mol/mol, which is sufficient to measure free sulfhydryl content in proteins including mAbs.
Yuling Zhang, Pei Qi

2974 related Products with: Determination of free sulfhydryl contents for proteins including monoclonal antibodies by use of SoloVPE.

1mg1mg1mg1 mg1mg10mg1 mg100.00 ug1 mg1 mg1 mg1mg

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#33984792   2021/05/04 To Up

Experimental efficacy of a trivalent vaccine containing porcine circovirus types 2a/b (PCV2a/b) and Mycoplasma hyopneumoniae against PCV2d and M. hyopneumoniae challenges.

The purpose of this experimental study was to evaluate the efficacy of a new trivalent vaccine containing porcine circovirus types 2a/b (PCV2a/b) and Mycoplasma hyopneumoniae. Pigs were administered the vaccine intramuscularly as either at 3 and 24 days of age with 1.0 mL or at 21 days of age with 2.0 mL according to the manufacturer's recommendations. The pigs were challenged at 42 days of age with either PCV2d (intranasal route) or M. hyopneumoniae (intratracheal route), or both. No statistical differences were observed between the one-dose and two-dose experiments based on clinical (growth performance), immunological (protective immunity), microbiological (viremia and laryngeal swab), and pathological (pulmonary and lymphoid lesion) outcomes. Pigs in vaccinated/challenged and unvaccinated/unchallenged groups showed significant difference in growth performance compared to pigs in the unvaccinated/challenged group in both dosage experiments. Vaccinated pigs elicited a significant amount of protective immunity for PCV2d-specific neutralizing antibodies and interferon-γ secreting cells (IFN-γ-SC) as well as M. hyopneumoniae-specific IFN-γ-SC significantly post-challenge compared to unvaccinated/challenged pigs. Vaccination and challenge reduced the viral load amount of PCV2d in the blood and reduced the M. hyopneumoniae load in laryngeal swab, while simultaneously reducing both pulmonary and lymphoid lesion severity when compared to unvaccinated/challenged pigs. Trivalent vaccination provided good protection against a single PCV2d challenge, single M. hyopneumoniae challenge, and a PCV2d/M. hyopneumoniae dual challenge.
Siyeon Yang, Taehwan Oh, Kee Hwan Park, Hyejean Cho, Jeongmin Suh, Chanhee Chae

1563 related Products with: Experimental efficacy of a trivalent vaccine containing porcine circovirus types 2a/b (PCV2a/b) and Mycoplasma hyopneumoniae against PCV2d and M. hyopneumoniae challenges.

25 mg96T1000 TESTS/0.65ml 5 G100 mg25 mg1000 tests100ul200ul1 ml

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#33984553   2021/05/10 To Up

Burosumab treatment for fibrous dysplasia.

Fibrous dysplasia/McCune-Albright syndrome (FD/MAS) is a rare mosaic disorder of Gα activation. Fibroblast Growth Factor 23 (FGF23)-mediated hypophosphatemia is a feature of FD/MAS that has been associated with poor skeletal outcomes. Standard therapy includes oral phosphorus and vitamin D analogs; however, treatment is limited by potential adverse renal and gastrointestinal effects. Burosumab is a monoclonal antibody to FGF23 approved to treat patients with X-linked hypophosphatemia and tumor-induced osteomalacia. There is currently no safety or efficacy data to support burosumab use in patients with FD/MAS.
Anne Gladding, Vivian Szymczuk, Bethany A Auble, Alison M Boyce

2552 related Products with: Burosumab treatment for fibrous dysplasia.

2x5L 100 G0.25 mgeach50 ml50 mL96 Well 1 G1 mg250 mg

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#33984507   2021/05/10 To Up

HisPhosSite: A comprehensive database of histidine phosphorylated proteins and sites.

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Jian Zhao, Lingxiao Zou, Yan Li, Xiaofei Liu, Cong Zeng, Chen Xu, Bin Jiang, Xuejiang Guo, Xiaofeng Song

2457 related Products with: HisPhosSite: A comprehensive database of histidine phosphorylated proteins and sites.

100ul21mg0.05mg1 mg1001005250 mg1mg20 10.00 ug

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#33984506   2021/05/10 To Up

Proteomic analysis of ubiquitinated proteins in maize immature kernels.

Protein ubiquitination is a dynamic post-translational modification involved in various biological processes in eukaryotes. To understand the function of ubiquitinated proteins in maize kernels, we used the specific K-GG antibody coupled with high-resolution LC-MS/MS to identify the ubiquitinated proteins in maize immature kernels. A total of 1999 lysine ubiquitination sites in 881 proteins were identified in maize kernels. Eight conserved ubiquitination motifs included KD, GK, EK, KXXXE, AK, NXK, KXXXXXN, and KK were found in ubiquitinated peptides. The ubiquitinated lysine neighborhoods are more frequently presented in ordered structures. Go and KEGG analysis showed the proteins involved in carbohydrate metabolism and protein processing were identified to be the targets of lysine ubiquitination. Other proteins, which related to RNA transport, spliceosome, endocytosis, ubiquitin-mediated proteolysis, proteasome, and MAPK signaling, were also found to be ubiquitinated. Protein-protein interaction network and KEGG analysis indicated that protein ubiquitination plays a major role in regulating many cellular processes and modulating diverse interactions in maize kernel development. The identification of the 881 ubiquitinated proteins in maize kernels provides a foundation for understanding the physiological roles of these ubiquitinated proteins. Our finding also provides a new insight view into the function of ubiquitinated proteins involved in maize kernel development. SIGNIFICANCE: We reported here the comprehensive proteomic analysis of the ubiquitin-modified proteome in maize kernel. We found that there are some new characteristics of them in ubiquitome of maize immature kernels. The results suggested that protein ubiquitination, as a post-translation modification, plays an essential role in regulating many cellular processes in maize kernel development. This study expands our knowledge on the regulatory roles and mechanisms of protein ubiquitination in maize. and other plants.
Wei Fan, Hongjian Zhen, Gang Wang

1597 related Products with: Proteomic analysis of ubiquitinated proteins in maize immature kernels.

101001mg1mg1001mg10101050 101mg

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#33984501   2021/05/10 To Up

The PAR promoter expression system: modified lac promoters for controlled recombinant protein production in Escherichia coli.

Many commonly used bacterial promoters employed for recombinant protein production (RPP) in Escherichia coli are capable of high-level protein expression. However, such promoter systems are often too strong, being ill suited for expressing proteins that are difficult to fold, targeted to the membrane or secreted out of the cytoplasm. To circumvent this problem, a suite of bacterial promoters has been constructed with a range of different promoter strengths, assigning them specific "promoter activity ratings" (PARs). Selecting three of these PAR promoters, with low, intermediate and high strengths, it is demonstrated that the expression of target proteins, such as green fluorescent protein (GFP), human growth hormone (hGH) and single chain variable region antibody fragments (scFvs), can be set to three levels when expressed in E. coli. It is shown that the PAR promoter system is extremely flexible, operating in a variety of E. coli strains and under various different culture regimes. Furthermore, due to its tight regulation, it is shown that this system can also express a toxic outer membrane protein, at levels which do not affect bacterial growth. Thus, the PAR promoter system can be used to tailor the expression levels of target proteins in E. coli and maximize RPP.
Joanne Hothersall, Rita E Godfrey, Christos Fanitsios, Tim W Overton, Stephen J W Busby, Douglas F Browning

2594 related Products with: The PAR promoter expression system: modified lac promoters for controlled recombinant protein production in Escherichia coli.

2x 100ug10ìg100 10 50 10010 50 50mg100 10 20

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#33984489   2021/05/10 To Up

Outbreak investigation of symptomatic SARS-COV-2 VOC 202012/01-lineage B.1.1.7 infection in healthcare workers, Italy.

In December 2020, Italy began a national immunization campaign using the BNT162b2 mRNA COVID-19 vaccine, prioritizing healthcare workers (HCWs). Immune serum from vaccinated subjects seems to (largely) retain titres of neutralizing antibodies, even against SARS-CoV-2 VOC 202012/01-lineage B.1.1.7. Here, we describe an outbreak of SARS-CoV-2 lineage B.1.1.7 infection in three HCWs in a hospital setting; two of the HCWs were fully vaccinated (i.e., had received two doses).
Daniela Loconsole, Anna Sallustio, Marisa Accogli, Angela Leaci, Antonio Sanguedolce, Antonio Parisi, Maria Chironna

1248 related Products with: Outbreak investigation of symptomatic SARS-COV-2 VOC 202012/01-lineage B.1.1.7 infection in healthcare workers, Italy.

100 ul2000 Units5mg20 ul500 250 m Pcs Per Pack96 tests20mg10 mg100

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#33984393   2021/05/10 To Up

Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2.

The novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the worldwide coronavirus disease-19 (COVID-19) pandemic, starting in late 2019. The standard diagnostic methods to detect SARS-CoV-2 are PCR-based genetic assays. Antigen-antibody-based immunochromatographic assays are alternative methods of detecting this virus. Rapid diagnosis kits to detect SARS-CoV-2 are urgently needed.
Satoshi Oshiro, Yoko Tabe, Keiji Funatogawa, Kaori Saito, Tatsuya Tada, Tomomi Hishinuma, Naeko Mizutani, Makoto Akiwa, Jun-Ichiro Sekiguchi, Takashi Miida, Teruo Kirikae

2000 related Products with: Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2.

1 mg100 assays100 assays1 mL1 module100 ug/vial1 kit(96 Wells)0.1 mg1 module

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#33984384   2021/05/10 To Up

Identification of a novel bluetongue virus 1 specific B cell epitope using monoclonal antibodies against the VP2 protein.

Bluetongue (BT) is a non-contact infectious disease caused by Bluetongue virus (BTV), which can be transmitted by vector insects such as Culicoides and Aedes mosquitoes. The BTV VP2 protein encoded by the L2 gene is located at the outermost layer of the virus particle, plays a key role on mediating the adsorption and entry of virus, and it is also a main antigenic protein widely used for vaccine development. In this study, the BTV1 VP2 gene was cloned into pFastBac™Dual vector, and expressed in insect Sf21 cells. Immunized mice with purified recombinant VP2 protein can induce higher levels of antibodies. Three anti BTV1 VP2 monoclonal antibodies (mAbs) were generated (17E9C6, 17E9C8, 17E9H12), and showed high specific reactivity with recombinant VP2 protein and inactivated BTV1 virus. Finally, a novel linear B-cell epitope KEPAD on recombinant VP2 protein was identified by using three mAbs react with a series of continue-truncated peptides. The results of this study may provide new information on the structure and function of BTV1 VP2 protein and lay a foundation for the development of BTV1 diagnostic and prophylactic methods.
Aiping Wang, Jinran Du, Hua Feng, Jingming Zhou, Yumei Chen, Yankai Liu, Min Jiang, Rui Jia, Yuanyuan Tian, Gaiping Zhang

2420 related Products with: Identification of a novel bluetongue virus 1 specific B cell epitope using monoclonal antibodies against the VP2 protein.

100ug/vial100ug100ug100.00 ug100ul100 ug100ug100ul100ug100ug100.00 ug

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