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Search results for: Androstenedione-19 Antibody

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#34315173   2021/07/28 To Up

Aberrant glycosylation of anti-SARS-CoV-2 IgG is a pro-thrombotic stimulus for platelets.

A subset of patients with COVID-19 become critically ill, suffering from severe respiratory problems and also increased rates of thrombosis. The causes of thrombosis in severely ill COVID-19 patients are still emerging, but the coincidence of critical illness with the timing of the onset of adaptive immunity could implicate an excessive immune response. We hypothesised that platelets might be susceptible to activation by anti-SARS-CoV-2 antibodies and contribute to thrombosis. We found that immune complexes containing recombinant SARS-CoV-2 spike protein and anti-spike IgG enhanced platelet-mediated thrombosis on von Willebrand Factor in vitro, but only when the glycosylation state of the Fc domain was modified to correspond with the aberrant glycosylation previously identified in patients with severe COVID-19. Furthermore, we found that activation was dependent on FcγRIIA and we provide in vitro evidence that this pathogenic platelet activation can be counteracted by therapeutic small molecules R406 (fostamatinib) and ibrutinib that inhibit tyrosine kinases Syk and Btk respectively or by the P2Y12 antagonist cangrelor.
Alexander P Bye, Willianne Hoepel, Joanne L Mitchell, Sophie Mélanie Jégouic, Silvia Loureiro, Tanya Sage, Gestur Vidarsson, Jan Nouta, Manfred Wuhrer, Steven W de Taeye, Marit van Gils, Neline Kriek, Nichola Cooper, Ian Jones, Jeroen den Dunnen, Jonathan M Gibbins

2426 related Products with: Aberrant glycosylation of anti-SARS-CoV-2 IgG is a pro-thrombotic stimulus for platelets.

100 ul100 ul100 ul100 ul0.25 mg100 ul100 ul100 ul100 TESTS100 ul100 ul100 ul

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#34315114   2021/07/24 To Up

Anti-F4/80 treatment attenuates Th2 cell responses: Implications for the role of lung interstitial macrophages in the asthmatic mice.

Lung interstitial macrophages (IMs) can be polarized towards an alternative activation phenotype in ovalbumin (OVA)-induced asthmatic mice. However, the role of alternative activation of lung IMs in Th2 cell responses in the asthmatic murine is still unclear. Here, we leverage an anti-F4/80 treatment which has been shown to selectively deplete IMs in mice and investigate how this treatment modulates Th2 cell responses in lung and whether the modulation is dependent on lung IMs in murine models of asthma. We show that anti-F4/80 treatment alleviates Th2 cell responses in mice immunized and challenged with OVA or house dust mite (HDM). The anti-F4/80 treatment does not target lung alveolar macrophages (AMs) in OVA-induced asthmatic mice or impact the abundance of other immune cell types, including B cells, T cells, and NK cells in wild-type mice. However, this treatment does inhibit the expression of polarized markers of alternatively activated macrophages, including arginase-1, Ym-1, and Fizz-1 in the lung tissues from OVA-induced asthmatic mice. Furthermore, we find that the inhibitory effects of anti-F4/80 treatment on Th2 cell responses can be reversed upon adoptive transfer of lung IMs. Taken together, our data show that anti-F4/80 treatment attenuates Th2 cell responses, which is at least partially related to depletion of lung IMs in murine models of asthma. This suggests that targeted lung IMs may provide a potential therapeutic protocol for the treatment of asthmatics.
Nishan Deng, Xuxue Guo, Qianhui Chen, Linlin Liu, Shuo Chen, Ailing Wang, Ruiyun Li, Yi Huang, Xuhong Ding, Hongying Yu, Suping Hu, Yang Zhao, Xueqin Chen, Hanxiang Nie

1801 related Products with: Anti-F4/80 treatment attenuates Th2 cell responses: Implications for the role of lung interstitial macrophages in the asthmatic mice.

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#34315112   2021/07/20 To Up

The cross-talk between tumor cells and activated fibroblasts mediated by lactate/BDNF/TrkB signaling promotes acquired resistance to anlotinib in human gastric cancer.

Acquired resistance to tyrosine kinase inhibitors (TKIs) is the major obstacle to improve clinical efficacy in cancer patients. The epithelial-stromal interaction in tumor microenvironment influences cancer drug response to TKIs. Anlotinib is a novel oral multi-targeted TKI, and has recently been proven to be effective and safe for several tumors. However, if and how the epithelial-stromal interaction in tumor microenvironment affects anlotinib response in gastric cancer (GC) is not known. In this study, we found that anlotinib inhibited GC cells growth by inducing GC cells apoptosis and G2/M phase arrest in a dose- and time-dependent manner. Reactive oxygen species (ROS) mediated anlotinib-induced apoptosis in GC cells, while cancer-associated fibroblasts (CAFs) significantly suppressed anlotinib-induced apoptosis and ROS in GC cells. Increased BDNF that was derived from CAFs activated TrkB-Nrf2 signaling in GC cells, and reduced GC cells response to anlotinib. We identified secreted lactate from GC cells as the key molecule instructing CAFs to produce BDNF in a NF-κB-dependent manner. Additionally, functional targeting BDNF-TrkB pathway with neutralizing antibodies against BDNF and TrkB increased the sensitivity of GC cells towards anlotinib in human patient-derived organoid (PDO) model. Taken together, these results characterize a critical role of the epithelial-stroma interaction mediated by the lactate/BDNF/TrkB signaling in GC anlotinib resistance, and provide a novel option to overcome drug resistance.
Zhijian Jin, Yifan Lu, Xiongyan Wu, Tao Pan, Zhenjia Yu, Junyi Hou, Airong Wu, Jianfang Li, Zhongyin Yang, Chen Li, Min Yan, Chao Yan, Zhenggang Zhu, Bingya Liu, Weihua Qiu, Liping Su

1078 related Products with: The cross-talk between tumor cells and activated fibroblasts mediated by lactate/BDNF/TrkB signaling promotes acquired resistance to anlotinib in human gastric cancer.

1.00 flask10 100 μg1.00 flask25 100 ug/vial25 TESTS

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#34315016   2021/07/23 To Up

Contrast agent retention features in contrast-enhanced ultrasound: diagnostic performance for the prediction of papillary thyroid carcinoma.

To report a new feature of contrast-enhanced ultrasound (CEUS) and its diagnostic performance for the prediction of papillary thyroid carcinoma (PTC).
Zhuang Jin, Yaqiong Zhu, Fang Xie, Yan Zhang, Nan Li, Yukun Luo, Junying Cao

1856 related Products with: Contrast agent retention features in contrast-enhanced ultrasound: diagnostic performance for the prediction of papillary thyroid carcinoma.

500 Slides

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#34315013   2021/07/24 To Up

Current state of knowledge on immunotherapy in ECOG PS 2 patients. A systematic review.

Patients with Eastern Cooperative Oncology Group Performance Status 2 (ECOG PS 2) are not included in most randomized clinical trials and registry studies. Nevertheless, immune checkpoint inhibitors are registered in the USA and Europe regardless of the performance status. Evidence regarding the effectiveness and safety of such treatment in this cohort is sparse.
Damian Mojsak, Beata Kuklińska, Łukasz Minarowski, Robert Marek Mróz

2424 related Products with: Current state of knowledge on immunotherapy in ECOG PS 2 patients. A systematic review.

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#34315010   2021/07/18 To Up

Dynamics of breast milk antibody titer in the six months following SARS-CoV-2 infection.

While a growing body of literature describes antibody dynamics in serum, little is known about breast milk antibody titers in the months following SARS-CoV-2 infection.
Caroline J Duncombe, Denise J McCulloch, Kiel D Shuey, Jennifer K Logue, Nicholas M Franko, Caitlin R Wolf, Collrane J Frivold, Helen Y Chu

2357 related Products with: Dynamics of breast milk antibody titer in the six months following SARS-CoV-2 infection.

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#34315003   2021/07/12 To Up

Effects of immunosuppression on the efficacy of vaccination against Mycoplasma gallisepticum infection in chickens.

Immunosuppression can increase the susceptibility of chickens to other disease-causing pathogens and interfere with the efficacy of vaccination against those pathogens. Chicken anaemia virus (CAV) and infectious bursal disease virus (IBDV) are common causes of immunosuppression in chickens. Immunosuppression was induced by experimental infection with either CAV or IBDV to assess the effect of immunosuppression on the efficacy of vaccination with Mycoplasma gallisepticum strain ts-304 against infection with virulent M. gallisepticum, a common bacterial pathogen of chickens worldwide. Birds were experimentally infected with either CAV or IBDV at 1 week of age, before vaccination and challenge with M. gallisepticum to examine the effect of immunosuppression at the time of vaccination, or at 6 weeks of age, after vaccination against M. gallisepticum but before challenge with virulent M. gallisepticum, to investigate the effect of immunosuppression at the time of challenge. All birds were vaccinated with a single dose of the ts-304 vaccine at 3 weeks of age and experimentally challenged with the virulent M. gallisepticum strain Ap3AS at 8 weeks of age. In immunosuppressed chickens there was a reduction in protection offered by the ts-304 vaccine at two weeks after challenge, as measured by tracheal mucosal thicknesses, serum antibody levels against M. gallisepticum, air sac lesion scores and virulent M. gallisepticum load in the trachea. Immunosuppressed birds with detectable serum antibodies against M. gallisepticum were less likely to have tracheal lesions. This study has shown that immunosuppression caused by infection with CAV or IBDV can interfere with vaccination against mycoplasmosis in chickens.
Sathya N Kulappu Arachchige, Anna Kanci Condello, Ling Zhu, Pollob K Shil, Kelly A Tivendale, Gregory J Underwood, Amir H Noormohammadi, Glenn F Browning, Nadeeka K Wawegama

1629 related Products with: Effects of immunosuppression on the efficacy of vaccination against Mycoplasma gallisepticum infection in chickens.

5 G96 tests196 tests100ug

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#34314936   2021/07/22 To Up

Use of blood matrices and alternative biological fluids for antibody detection in animal tuberculosis.

Bovine tuberculosis (bTB) control programs can be improved by implementation of advanced ante-mortem testing algorithms. Serodiagnostic methods using traditional blood or blood-derived specimens may benefit from the use of less invasive alternative biological fluids, provided those mirror systemic antibody responses. In the present study, we used Dual Path Platform (DPP) and Multiantigen Print Immunoassay (MAPIA) to compare antibody levels in ten sample types including whole blood (fresh and hemolyzed), plasma (fresh and leftover from Bovigam testing), serum, saliva, broncho-alveolar lavage, urine, diaphragm extract, and bile collected from cattle aerosol-infected with Mycobacterium bovis. High correlation (r = 0.97-0.99) in measurements of IgG antibodies to MPB70/MPB83 fusion antigen by DPP assay was found between all blood-derived specimens, supporting matrix equivalency. Broncho-alveolar lavage and diaphragm extract yielded positive results in all the infected animals tested, showing high correlation with matching serum data (r = 0.94 and r = 0.95, respectively) and suggesting their potential use in antibody assays. Characterized by MAPIA, the antigen reactivity patterns obtained with paired sera and alternative specimens were nearly identical, with slight differences in intensity. Antibodies were also found by DPP assay in saliva, urine, and bile from some of the infected animals, but the titers were relatively low, thus reducing the diagnostic value of such specimens. The proposed approach was evaluated in a pilot field study on warthogs diagnosed with M. bovis infection. Relative levels of antibody in tissue fluid obtained from lymph nodes or lungs were consistent with those detected in sera and detectable in all infected warthogs. The findings support the diagnostic utility of non-traditional biological fluids and tissue samples when used as alternative test specimens in serologic assays for bTB.
Konstantin P Lyashchenko, Alina Sikar-Gang, Archana A Sridhara, Ashley Johnathan-Lee, Rubyat Elahi, Rena Greenwald, Paul Lambotte, Javan Esfandiari, Eduard O Roos, Tanya J Kerr, Michele A Miller, Tyler C Thacker, Mitchell V Palmer, W Ray Waters

1672 related Products with: Use of blood matrices and alternative biological fluids for antibody detection in animal tuberculosis.

100 UG1 Set1 Set1 Set1 Set1 Set 100ul1 Set1 Set100ug100ug Lyophilized100ug Lyophilized

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#34314911   2021/07/24 To Up

P300 inhibition improves cell apoptosis and cognition impairment induced by sevoflurane through regulating IL-17A activation.

Sevoflurane (Sev) is a rapidly acting, potent inhalation anesthetic with rapid uptake and elimination. But many studies showed Sev could result in cognition dysfunction in adolescent or aging patients. Now, therapeutic targeting with IL-17A antibody showed a promising effect on Sev induced cognition impairment. In the study we reported that P300 inhibition could act as an alternative approach to decrease IL-17A activity. We found that P300 and IL-17A were activated in SHSY5Y cells treated with Sev. P300 inhibitor C646 could reduce the apoptosis induced by Sev through decreasing IL-17A avtivity. Furthermore, IL-17A expression was up-regulated after neurons were transfected with P300 expression plasmid and IL-17A expression was down-regulated after neurons were incubated with P300 inhibitor C646. P300 over-expression could up-regulate the promoter activity of IL-17A. Finally, in rat model treated with Sev, we also found C646 significantly improve the cognition impairment through IL-17A pathway. These data showed that P300 will potentially be a new drug target for the therapy of cognition impairment caused by Sev.
An Chen, Binbin Tan, Yifeng Cheng

2695 related Products with: P300 inhibition improves cell apoptosis and cognition impairment induced by sevoflurane through regulating IL-17A activation.

100ug1 kit1 kit1 kit1 kit100 Tests100ug100 Tests96 assays1 kit400 ug1 kit

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