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#33631842   2021/02/25 To Up

Pharmacological corrections of the mutant hERG channels by posaconazole.

Properties of mutant human ether-à-go-go-related gene (hERG) channels can be modified by some antibiotics. However, the pharmacological effects of posaconazole on cardiomyocyte hERG channels remain unclear. Whole-cell patch clamping, western blotting and laser confocal scanning microscopy were used to evaluate the effects of posaconazole on wild-type (WT)-, A561V- and L539 fs/47-hERG channels expressed in human embryonic kidney (HEK) 293 cells. In electrophysiological experiments, HEK 293 cells were transiently co-transfected with equal amounts of WT-hERG, WT+A561 V-hERG and WT+L539 fs/47-hERG plasmids to mimic a heterozygous genotype. Posaconazole (30 μM) increased tail currents in cells expressing WT-hERG, WT+A561 V-hERG and WT+L539 fs/47-hERG by 82.65%, 147.72% and 134.73%, respectively, compared to controls. Posaconazole increased hERG protein expression in cells expressing WT-hERG, WT+A561 V-hERG and WT+L539 fs/47-hERG compared to controls condition as well as their trafficking to the cell membrane. To our knowledge, this is the first study to show that antifungal agent posaconazole rescues the mutant A561 V-hERG and L539 fs/47-hERG channels by altering the gating kinetics, enhancing the expression and trafficking of hERG channels. The results demonstrate that posaconazole could be a promising candidate for the prevention and treatment of long QT syndrome and other arrhythmia-related diseases.
Chaodi Luo, Xinglong Zheng, Jing Li, Yongjian Zhang, Tao Shi, Yang Yan, Dan Han

2877 related Products with: Pharmacological corrections of the mutant hERG channels by posaconazole.

250ul5 10.5mg2100.00 ul250ul10

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#33631789   2021/02/26 To Up

Fucosylation and sialylation of Fc-Fragment of anti-TNF alpha antibodies do not influence their immunogenicity in monocyte-derived dendritic cells.


Marcin Wawrzyniak, Yasser Morsy, Radoslav Mladenov, Giulia Tontodonati, Yagmur Turgay, Stefan Herwig, Jörg Schneider, Jonathan Back, Manuela Mally, Amirreza Faridmoayer, Michael Scharl

2077 related Products with: Fucosylation and sialylation of Fc-Fragment of anti-TNF alpha antibodies do not influence their immunogenicity in monocyte-derived dendritic cells.

2 ml100 μg100.00 ug100 μg100 μg100 μg100.00 ug100 μg1000 100 μg100.00 ug25

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#33631727   2021/02/25 To Up

Magnetic ground states of Ce3TiSb5, Pr3TiSb5 and Nd3TiSb5 determined by neutron powder diffraction and magnetic measurements.

The R3TiSb5 ternary compounds, with R a light rare earth (La to Sm) have been reported to crystallize with the anti-Hf5CuSn3-type hexagonal structure (Pearson's symbol hP18; space-group P63/mcm, N. 193). An early article that reported possible superconductivity in some of these intermetallic phases (namely those with R = La, Ce, and Nd) caught our attention. In this work, we have now refined the crystal structure of the R3TiSb5 compounds with R = Ce, Pr and Nd by Rietveld methods using high-resolution neutron powder diffraction data. The magnetic ground states of these intermetallics have been investigated by low-temperature magnetization and high-intensity neutron diffraction. We find two different magnetic transitions corresponding to two related magnetic structures at TN1 = 4.8 K (k1 = [0,½,⅛]) and TN2 = 3.4 K (k2 = [0,0,⅛]), respectively for Ce3TiSb5. However, the magnetic ordering appears to occur following a peculiar hysteresis: the k2-type magnetic structure develops only after the k1-type phase fraction has first slowly ordered with time and the size of the ordered Ce3+ magnetic moment has become large enough to induce the second magnetic transition. At T = 1.5 K the maximum amplitude of the Ce moment in the coexisting phases amounts to μCe= 2.15 μB. For Nd3TiSb5 an antiferromagnetic ordering below TN = 5.2 K into a relatively simpler commensurate magnetic structure with a magnetic moment of μNd = 2.14(3) μB and magnetic propagation vector of k = [0,0,0], was determined. No evidence of superconductivity has been found in Nd3TiSb5. Finally, Pr3TiSb5 does not show any ordering down to 1.5 K in neutron diffraction while an antiferromagnetic ground state is detected in magnetization measurements. There is no sign of magnetic contribution from Ti atoms found in any of the studied compounds.
Clemens Ritter, Arjun Kumar Pathak, Randall Filippone, Alessia Provino, Sudesh Kumar Dhar, Pietro Manfrinetti

2984 related Products with: Magnetic ground states of Ce3TiSb5, Pr3TiSb5 and Nd3TiSb5 determined by neutron powder diffraction and magnetic measurements.

1500 mg25 mg2 mL at 10 mg/mL50 ug 10 mg100ug100ul25 mg 5 G1 mL at 10 mg/mL5mg

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#33631656   2021/02/16 To Up

Loop-mediated fluorescent probes for selective discrimination of parallel and antiparallel G-Quadruplexes.

Herein we report simple pyridinium (1-3) and quinolinium (4) salts for the selective recognition of G-quadruplexes (G4s). Among them, the probe 1, interestingly, selectively discriminated parallel (c-KIT-1, c-KIT-2, c-MYC) G4s from anti-parallel/hybrid (22AG, HRAS-1, BOM-17, TBA) G4s at pH 7.2, through a switch on response in the far-red window. Significant changes in the absorption (broad 575 nm → sharp 505 nm) and emission of probe 1 at 620 nm, attributed to selective interaction with parallel G4s, resulted in complete disaggregation-induced monomer emission. Symmetrical push/pull molecular confinements across the styryl units in probe 1 enhanced the intramolecular charge transfer (ICT) by restricting the free rotation of CC units in the presence of sterically less hindered and highly accessible G4 surface/bottom tetrads in the parallel G4s, which is relatively lower extent in antiparallel/hybrid G4s. We confirm that the disaggregation of probe 1 was very effective in the presence of parallel G4-forming ODNs, due to the presence of highly available free surface area, resulting in additional π-stacking interactions. The selective sensing capabilities of probe 1 were analyzed using UV-Vis spectroscopy, fluorescence spectroscopy, molecular dynamics (MD)-based simulation studies, and H NMR spectroscopy. This study should afford insights for the future design of selective compounds targeting parallel G4s.
Anup Pandith, Upendra Nagarajachari, Ravi Kumara Guralamatta Siddappa, Sungjin Lee, Chin-Ju Park, Krishnaveni Sannathammegowda, Young Jun Seo

1133 related Products with: Loop-mediated fluorescent probes for selective discrimination of parallel and antiparallel G-Quadruplexes.

10reactions 10reactions 10reactions 10reactions 10reactions 1 mg10 mg25 μg1 g

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#33631652   2021/02/03 To Up

Astrocyte-derived extracellular vesicles enhance the survival and electrophysiological function of human cortical neurons in vitro.

Neurons derived from human induced pluripotent stem cells (hiPSCs) are powerful tools for modeling neural pathophysiology and preclinical efficacy/toxicity screening of novel therapeutic compounds. However, human neurons cultured in vitro typically do not fully recapitulate the physiology of the human nervous system, especially in terms of exhibiting morphological maturation, longevity, and electrochemical signaling ability comparable to that of adult human neurons. In this study, we investigated the potential for astrocyte-derived extracellular vesicles (EVs) to modulate survival and electrophysiological function of human neurons in vitro. Specifically, we demonstrate that EVs obtained from human astrocytes promote enhanced single cell electrophysiological function and anti-apoptotic behavior in a homogeneous population of human iPSC-derived cortical neurons. Furthermore, EV-proteomic analysis was performed to identify cargo proteins with the potential to promote the physiological enhancement observed. EV cargos were found to include neuroprotective proteins such as heat shock proteins, alpha-synuclein, and lipoprotein receptor-related protein 1 (LRP1), as well as apolipoprotein E (APOE), which negatively regulates neuronal apoptosis, and a peroxidasin homolog that supports neuronal oxidative stress management. Proteins that positively regulate neuronal excitability and synaptic development were also detected, such as potassium channel tetramerization domain containing 12 (KCTD12), glucose-6- phosphate dehydrogenase (G6PD), kinesin family member 5B (KIF5B), spectrin-alpha non-erythrocytic1 (SPTAN1). The remarkable improvements in electrophysiological function and evident inhibition of apoptotic signaling in cultured neurons exposed to these cargos may hold significance for improving preclinical in vitro screening modalities. In addition, our collected data highlight the potential for EV-based therapeutics as a potential class of future clinical treatment for tackling inveterate central and peripheral neuropathies.
Changho Chun, Alec S T Smith, Hyejin Kim, Dana S Kamenz, Jung Hyun Lee, Jong Bum Lee, David L Mack, Mark Bothwell, Claire D Clelland, Deok-Ho Kim

2574 related Products with: Astrocyte-derived extracellular vesicles enhance the survival and electrophysiological function of human cortical neurons in vitro.

96 tests96 tests96 tests96 tests100 μg100 μg 100ul100 ul100 μg100 μg1 mg100 μg

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#33631650   2021/02/22 To Up

Short-chain fatty acid butyrate induces IL-10-producing B cells by regulating circadian-clock-related genes to ameliorate Sjögren's syndrome.

Sjögren's syndrome (SS) is an autoimmune disease caused by inflammation of the exocrine gland. The pathological hallmark of SS is the infiltration of lymphocytes into the salivary glands. Increased infiltration of T and B cells into salivary glands exacerbates symptoms of SS. Several recent studies have identified the role of gut microbiota in SS. Butyrate, one of the metabolites of the gut microbiota, regulates T cells; however, its effects on B cells and SS remain unknown. This study determined the therapeutic effect of butyrate on regulating B cells in SS.
Da Som Kim, Jin Seok Woo, Hong-Ki Min, Jeong-Won Choi, Jeong Hyeon Moon, Min-Jung Park, Seung-Ki Kwok, Sung-Hwan Park, Mi-La Cho

1753 related Products with: Short-chain fatty acid butyrate induces IL-10-producing B cells by regulating circadian-clock-related genes to ameliorate Sjögren's syndrome.

10 mg100 extractions10 500 mg96 tests430 tests 1 G25 mg15ml100ug Lyophilized430 Tests / Kit

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#33631643   2021/01/07 To Up

Value of next-generation sequencing in early diagnosis of patients with tuberculous meningitis.

To assess the value of next-generation sequencing (NGS) technology in early diagnosis of patients with tuberculous meningitis (TBM).
Aiqing Lin, Baotao Cheng, Xiaochun Han, Hong Zhang, Xiaoli Liu, Xueping Liu

2908 related Products with: Value of next-generation sequencing in early diagnosis of patients with tuberculous meningitis.

5 G96T96TInhibitor96/kit50 ug 100ug100 μg5ug

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#33631613   2021/02/16 To Up

Can sucrose-substitutes increase the antagonistic activity against foodborne pathogens, and improve the technological and functional properties of sheep milk kefir?

The effect of different types of sugar (sucrose, demerara, brown, fructose, coconut sugar, and honey) on sheep milk kefir was evaluated. Microbial counts (Lactobacillus, Lactococcus, Leuconostoc, yeast), antagonistic activity against foodborne pathogens, microstructure (scanning electron microscopy), and antiproliferative activity of cancer cells were evaluated. Furthermore, the antioxidant activity (DPPH), inhibitory activity of angiotensin-converting enzyme (ACE), α-amylase, and α-glucosidase, lactose content, lactic and acetic acids and ethanol, fatty acid profile and volatile organic compounds were determined. The addition of sugars increased the Lactobacillus population (up to 2.24 log CFU/mL), metabolites concentration, antagonistic activity against pathogens, antioxidant activity (11.1 to 24.1%), ACE inhibitory activity (27.5 to 37.6%), α-amylase inhibition (18 to 37.4%), and anti-proliferative activity. Furthermore, it improved the fatty acid profile and volatile compounds. The results suggest that the replacement of sucrose with different types of sugar constitute an interesting option to be used in sheep milk kefir formulations.
Cristiane P Larosa, Celso F Balthazar, Jonas T Guimarães, Larissa P Margalho, Felipe S Lemos, Felipe L Oliveira, Yuri K D Abud, Celso Sant'Anna, Maria Carmela K H Duarte, Daniel Granato, Renata S L Raices, Monica Q Freitas, Anderson S Sant'Ana, Erick Almeida Esmerino, Tatiana C Pimentel, Marcia Cristina Silva, Adriano G Cruz

1265 related Products with: Can sucrose-substitutes increase the antagonistic activity against foodborne pathogens, and improve the technological and functional properties of sheep milk kefir?

1 ml100 mg 25 MG200 units1 mg25 mg1200ug

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#33631602   2021/02/12 To Up

Stage-dependent sequential organization of nascent smooth muscle cells and its implications for the gut coiling morphogenesis in Xenopus larva.

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Kaoru Akinaga, Yoshitaka Azumi, Kazue Mogi, Ryuji Toyoizumi

1157 related Products with: Stage-dependent sequential organization of nascent smooth muscle cells and its implications for the gut coiling morphogenesis in Xenopus larva.

1x10e7 cells

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