Search results for: Anti-ACSL3(long-chain fatty-acid-Coenzyme A ligase 3) Antibody
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Blood cell gene expression profiling in rheumatoid arthritis. Discriminative genes and effect of rheumatoid factor.
To study the pathogenic importance of the rheumatoid factor (RF) in rheumatoid arthritis (RA) and to identify genes differentially expressed in patients and healthy individuals, total RNA was isolated from peripheral blood mononuclear cells (PBMC) from eight RF-positive and six RF-negative RA patients, and seven healthy controls. Gene expression of about 10,000 genes were examined using oligonucleotide-based DNA chip microarrays. The analyses showed no significant differences in PBMC expression patterns from RF-positive and RF-negative patients. However, comparisons of gene expression patterns from all fourteen RA patients and healthy controls identified a subset of discriminative genes. These results were validated by real time reverse transcription polymerase chain reaction (RT-PCR) on another group of RA patients and healthy controls. This confirmed that the following genes had a significantly higher expression in RA patients than in healthy controls: CD14 antigen, defensin alpha-1 and alpha-3 (DEFA), fatty-acid-Coenzyme A ligase, long-chain 2 (FACL), ribonuclease 2 (RNASE2), S100 calcium-binding protein A8 and A12 (S100A8 and S100A12). In contrast, the expression of MHC class II, DQ beta1 (HLA-DQB1) was significantly reduced in RA patients compared to healthy controls.Lone Frier Bovin, Klaus Rieneck, Christopher Workman, Henrik Nielsen, Søren Freiesleben Sørensen, Henrik Skjødt, Adrian Florescu, Søren Brunak, Klaus Bendtzen
2401 related Products with: Blood cell gene expression profiling in rheumatoid arthritis. Discriminative genes and effect of rheumatoid factor.
300 units 25UG10 ug1 mg96 wells (1 kit)1 mL96 wells (1 kit)100ug96 samplesRelated Pathways
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Glypican-3 is overexpressed in human hepatocellular carcinoma.
To identify candidate genes that could be used as diagnostic and therapeutic targets for hepatocellular carcinoma (HCC), we searched for the genes that are overexpressed in HCC by combining representational difference analysis and microarray. Genes such as glypican-3 (GPC3), insulin-like growth factor 2, long-chain fatty-acid-coenzyme A ligase 4, farnesyl diphosphate synthase were frequently identified in our screening. Northern blot analysis with these four genes confirmed their overexpression in HCC. Among them we found that GPC3 transcript is upregulated in six out of seven cases of HCC. Immunoblot and immunohistochemical staining using polyclonal anti-GPC3 antibodies further confirmed that GPC3 protein is indeed increased in HCC tumor samples. We also found that GPC3 is secreted into culture media from cell lines derived from HCC. We conclude that GPC3 is a good molecular marker for HCC.Young Kwan Sung, Sun Young Hwang, Mi Kyung Park, Mohammad Farooq, In Sook Han, Han Ik Bae, Jung-Chul Kim, Moonkyu Kim
1636 related Products with: Glypican-3 is overexpressed in human hepatocellular carcinoma.
100ug Lyophilized100 μg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100 μg100ug Lyophilized100ug LyophilizedRelated Pathways
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