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Search results for: Anti-BMPR1A(Bone morphogenetic protein receptor type IA) Antibody

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#32022225   2020/01/31 To Up

Smad1/5 signal transduction regulates the ameloblastic differentiation of induced pluripotent stem cells.

Induced pluripotent stem (iPS) cells could be induced into ameloblast-like cells by ameloblasts serum-free conditioned medium (ASF-CM), and bone morphogenetic proteins (BMPs) might be essential during the regulation of this process. The present study investigates the signal transduction that regulates the ameloblastic differentiation of iPS cells induced by ASF-CM. Mouse iPS cells were characterized and then cultured for 14 days in epithelial cell medium (control) or ASF-CM. Bone morphogenetic protein receptor II (BMPR-II) siRNA, inhibitor of Smad1/5 phosphorylation activated by activin receptor-like kinase (ALK) receptors, and inhibitors of mitogen-activated protein kinases (MAPKs) phosphorylation were used to treat the iPS cells in combination with ASF-CM. Real-time PCR, western blotting, and immunofluorescent staining were used to evaluate the expressions of ameloblast markers ameloblastin, enamelin, and cytokeratin-14. BMPR-II gene and protein levels increased markedly in ASF-CM-treated iPS cells compared with the controls, while the mRNA levels of Bmpr-Ia and Bmpr-Ib were similar between the ASF-CM and control groups. ASF-CM stimulation significantly increased the gene and protein expression of ameloblastin, enamelin and cytokeratin-14, and phosphorylated SMAD1/5, p38 MAPK, and ERK1/2 MAPK compared with the controls. Knockdown of BMPR-II and inhibition of Smad1/5 phosphorylation both could significantly reverse the increased expression of ameloblastin, enamelin, and cytokeratin-14 induced by ASF-CM, while neither inhibition of p38 nor ERK1/2 phosphorylation had significant reversing effects. We conclude that smad1/5 signaling transduction, activated by ALK receptors, regulates the ameloblastic differentiation of iPS cells induced by ameloblast-conditioned medium.
Zhi Liu, Guang Zeng, Qing Qin, Cong Sun, Lei Tan

1570 related Products with: Smad1/5 signal transduction regulates the ameloblastic differentiation of induced pluripotent stem cells.

1.5x10(6) cells20 x 50ul/Unit10 ug5x10 ug50ml24 wells10 x 50ul/Unit100ug/vial1 x 10^6 cells/vial500IU50ml1SET

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#22762034   2012/04/18 To Up

Mutually inductive interactions between the lens and retina require ALK3 functions during mouse embryonic development.

To investigate the mutually inductive interactions that occur between the lens and retinal tissue during the development of the vertebrate eye.
Qi Zhao, Jiang-Yue Zhao, Di Wu, Xiang-Chuan Lu, Jin-Song Zhang, Zhi-Yu Zhang

1084 related Products with: Mutually inductive interactions between the lens and retina require ALK3 functions during mouse embryonic development.

100ug100ug0.1ml (1mg/ml)100.00 ug0.1ml100 50ul1 x 10^6 cells/vial0.1 mg

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#20927405   2010/09/28 To Up

A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.

Members of the TGF-β superfamily are characterized by a highly promiscuous ligand-receptor interaction as is readily apparent from the numeral discrepancy of only seven type I and five type II receptors available for more than 40 ligands. Structural and functional studies have been used to address the question of how specific signals can be deduced from a limited number of receptor combinations and to unravel the molecular mechanisms underlying the protein-protein recognition that allow such limited specificity.
Stefan Harth, Alexander Kotzsch, Junli Hu, Walter Sebald, Thomas D Mueller

1944 related Products with: A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.