Search results for: Anti elisa rec
#32350593 2020/04/30 To Up
Rapid noninvasive detection of bladder cancer using survivin antibody-conjugated gold nanoparticles (GNPs) based on localized surface plasmon resonance (LSPR).Bladder cancer is diagnosed by the use of several biomarkers, including survivin. This protein has an important role in the cancer progression by controlling the rate of cell apoptosis. Findings show that there is no survivin in normal tissues, whereas the level of survivin expression increases in tumor cells.
Mir Hadi Jazayeri, Tayebe Aghaie, Reza Nedaeinia, Mostafa Manian, Hamid Nickho
2339 related Products with: Rapid noninvasive detection of bladder cancer using survivin antibody-conjugated gold nanoparticles (GNPs) based on localized surface plasmon resonance (LSPR).100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug100ug100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug
#31909895 2020/01/07 To Up
Serum proteins TGFBI, PCSK9, and CCL14 are potential biomarkers for different traditional Chinese medicine syndromes of multidrug-resistant tuberculosis.Patients with multidrug-resistant tuberculosis (MDR-TB) tend to have a long course of anti-TB treatment and severe side effects. Traditional Chinese Medicine (TCM) has a synergistic effect in attenuation of MDR-TB. However, the lack of objective biological standards to classify and diagnose MDR-TB TCM syndromes could result in less effective TCM treatment. Therefore, in this study, we identified differentially expressed proteins (DEPs) in serum of individuals with MDR-TB TCM syndromes by applying isobaric tags for relative and absolute quantification coupled with two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) method and bioinformatics analysis. The functional analysis of DEPs was also performed. Additionally, DEPs among three different TCM syndromes of MDR-TB were validated by enzyme-linked immunosorbent assay (ELISA). Finally, a receiver operating characteristic (ROC) curve was performed to estimate the diagnostic ability of DEPs. A total of 71 DEPs were identified in the three different MDR-TB TCM syndrome groups such as the pulmonary Yin deficiency (PYD) syndrome group, the Hyperactivity of Fire due to Yin deficiency (HFYD) syndrome group, and the deficiency of Qi and Yin (DQY) syndrome group. The results showed that the expression level of transforming growth factor-beta-induced protein ig-h3 (TGFBI) was lower in the PYD syndrome group (p = .002), the proprotein convertase subtilisin/kexin type 9 (PCSK9) was overexpressed in the HFYD syndrome group (p < .0001), and the C-C motif chemokine ligand 14 (CCL14) expression level was reduced in the DQY syndrome group (p = .004). Our study demonstrated that serum TGFBI, PCSK9, and CCL14 may serve as potential novel biomarkers for PYD syndrome, HFYD syndrome and DQY syndrome of MDR-TB, respectively. The study provides a biological basis for MDR-TB TCM syndromes classification and can be of great significance for the treatment of different TCM syndromes.
Yu-Ting Hu, Wen-Jing Yi, Ting-Ting Jiang, Hui-Hui Tu, Li-Liang Wei, Li-Ying Shi, Chang-Ming Liu, Jing Chen, Yu-Shuai Han, Lin Gan, Zhi-Bin Li, Huai Huang, Ji-Cheng Li
2821 related Products with: Serum proteins TGFBI, PCSK9, and CCL14 are potential biomarkers for different traditional Chinese medicine syndromes of multidrug-resistant tuberculosis.1001mg1mg96T200 50 mL21mg100,000IU50101mg
#31565228 2019/09/03 To Up
Sheep pestivirus in Morocco: sero-epidemiological and molecular study.The present study is the first to investigate Border disease caused by the sheep pestivirus (SPV) in sheep herds in Morocco. Sero-epidemiological investigations were carried out in six regions of the Kingdom, known as important in terms of sheep breeding. A total of 760 blood samples were collected including aborted ewes from 28 randomly selected farms. The samples were analysed, for the determination of anti-pestivirus antibodies, using indirect ELISA technique. Next, reverse transcriptase PCR (RT-PCR) was conducted on serologically negative samples to identify possible persistently infected (PI) animals, through detection of specific RNA fragment. The results revealed an overall SPV seroprevalence in studied areas of 28.9%. The difference in seroprevalence between the six investigated regions was not statistically significant (p>0.05) and varied slightly from 20.9% to 37.5%. Furthermore, 93% of investigated farms were affected with an average seroprevalence of 22.7% (with a variation of 1%-74%). RT-PCR results were all negative, indicating the absence of PI animals in the tested samples. Nevertheless, the present study revealed that SPV is endemic in Morocco.
Ouafaa Fassi Fihri, Noâma Jammar, Nadia Amrani, Ikhlass El Berbri, Said Alali96T1 ml1 ml1mg0.1 ml100ug96 wells (1 kit)4 Arrays/Slide1 Set100 μg
#31350726 2019/07/26 To Up
Exposure to Ascaris lumbricoides infection and risk of childhood asthma in north of Iran.Asthma and Ascaris lumbricoides infection are common health issues affecting 250 and 700 million people worldwide, respectively. The relationship between ascariasis and asthma is a matter of substantial interest and research.
Iraj Mohammadzadeh, Ali Rostami, Sorena Darvish, Saeed Mehravar, Mohammad Pournasrollah, Mostafa Javanian, Mohammadreza Esmaeili Dooki, H Ray Gamble
1867 related Products with: Exposure to Ascaris lumbricoides infection and risk of childhood asthma in north of Iran.1 kit 1 G1mg1 mg100 μg500 gm.100 mg5096 tests
#31120191 2019/06/12 To Up
Killing Effects of IFN R Mouse NK Cells Activated by HN Protein of NDV on Mouse Hepatoma Cells and Possible Mechanism with Syk and NF-κB.The objective of this article is to evaluate whether the tumoricidal activity of mouse IFN R nature killer (NK) cells is induced by Newcastle disease virus hemagglutinin-neuraminidase (NDV-HN) stimulation, and to investigate what is the mechanism of the HN-stimulated NK cells to kill mouse hepatoma cell line in vitro. The mouse IFN R NK cells were stimulated for 16 hr with 500 ng/mL NDV-HN in 1640 medium. Quantify the cytotoxic activities of NK cells against mouse hepatoma cells (Hepa1-6) by flow cytometry. Granzymes B (GrB) and Fas/FasL concentrations in the supernatants of IFN R NK cells medium were determined by specific ELISA assay. The expression of cell surface GrB and Fas was determined by Western blot. NDV-HN stimulation enhanced tumoricidal activity of IFN R NK cells toward Hepa1-6 in vitro. Treating with anti-HN neutralizing mAb induced significant decline in the cytotoxicity of IFN R NK cells toward Hepa1-6 cell line (P < 0.05). After treating with anti-HN protein (1 μL/mL), Syk-specific inhibitor Herbimycin A(250 ng/mL) and NF-κB inhibitor PDTC (500 ng/mL) downregulated the tumoricidal activity of HN-stimulated IFN R NK cells (P < 0.05). Moreover, significant suppressions in the production of GrB and Fas/FasL were observed in HN-stimulated IFN R NK cells (P < 0.05). Thus, we concluded that killer activation receptors pathway is involved in the IFN-γ-independent GrB and Fas/FasL expression of NDV-HN-stimulated IFN R NK cells, and these are activated by Syk and NF-κB. Anat Rec, 302:1718-1725, 2019. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association for Anatomy.
Shuang Liang, Xiao Lin, Ying Liang, Dezhi Song, Lei Zhang, Xiaohui Fan
2982 related Products with: Killing Effects of IFN R Mouse NK Cells Activated by HN Protein of NDV on Mouse Hepatoma Cells and Possible Mechanism with Syk and NF-κB.100.00 ug100ug100ug250 100.00 ug100.00 ug0.1ml (1mg/ml)0.25 mL1mg1mg1mg
#30674643 2019/01/23 To Up
Incidence of anti-Der f 2 and anti-Zen 1-specific immunoglobulin E antibodies in atopic dogs from South-East England.Recent studies from North America and continental Europe have reported Zen 1 as a major allergen in atopic dogs, and Der f 2 as a minor allergen. In contrast, Der f 2 is considered a major allergen in Japan. In this study, allergen-specific IgE against Der f 2, Zen 1 and crude (DF) was determined using ELISA assays in English atopic dogs. Serum samples were obtained from 59 dogs with non-seasonal atopic dermatitis. ELISA assays using horseradish peroxidase-labelled anti-dog IgE monoclonal antibody (Bethyl; A40-125P) and recombinant Der f 2 (Zenoaq), natural Zen 1 (Zenoaq) and DF extract (Greer Laboratories; North Carolina) were performed by Zenoaq, Fukushima, Japan. The mean optical density (OD) of each sample was determined and the cut-off value was calculated from OD readings obtained from four healthy control dogs. Der f 2, Zen 1 and DF-specific IgE antibodies were found in the serum samples taken from 57 (97 per cent), 45 (76 per cent) and 47 (80 per cent) atopic dogs, respectively, suggesting that both Zen 1 and Der f 2 are 'major allergens' in the South-East England.
Anita Patel, Cathy F Curtis, Rosario Cerundolo
2821 related Products with: Incidence of anti-Der f 2 and anti-Zen 1-specific immunoglobulin E antibodies in atopic dogs from South-East England.25mg100 μg100ug100 μg100 μg25 100 μg100 μg100.00 ug100.00 ug100 μg100 μg
#29246841 2017/12/12 To Up
Immunization with a nontoxic naturally occurring Clostridium perfringens alpha toxin induces neutralizing antibodies in rabbits.Clostridium perfringens alpha toxin, encoded by plc gene, has been implicated in gas gangrene, a life threatening infection. Vaccination is considered one of the best solutions against Clostridium infections. Although studies have identified many low quality clostridial vaccines, the use of recombinant proteins has been considered a promising alternative. Previously, a naturally occurring alpha toxin isoform (αAV1b) was identified with a mutation at residue 11 (His/Tyr), which can affect its enzymatic activity. The aim of the present study was to evaluate whether the mutation in the αAV1b isoform could result in an inactive toxin and was able to induce protection against the native alpha toxin. We used recombinant protein techniques to determine whether this mutation in αAV1b could result in an inactive toxin compared to the active isoform, αZ23. Rabbits were immunized with the recombinant toxins (αAV1b and αZ23) and with native alpha toxin. αAV1b showed no enzymatic and hemolytic activities. ELISA titration assays showed a high titer of both anti-recombinant toxin (anti-rec-αAV1b and anti-rec-αZ23) antibodies against the native alpha toxin. The alpha antitoxin titer detected in the rabbits' serum pool was 24.0 IU/mL for both recombinant toxins. These results demonstrate that the inactive naturally mutated αAV1b is able to induce an immune response, and suggest it can be considered as a target for the development of a commercial vaccine against C. perfringens alpha toxin.
Flávia de Faria Siqueira, Rodrigo Otávio Silveira Silva, Anderson Oliveira do Carmo, Bárbara Bruna Ribeiro de Oliveira-Mendes, Carolina Campolina Rebello Horta, Francisco Carlos Faria Lobato, Evanguedes Kalapothakis
1464 related Products with: Immunization with a nontoxic naturally occurring Clostridium perfringens alpha toxin induces neutralizing antibodies in rabbits.1 mg100.00 ug100.00 ug100 100.00 ug100.00 ug100 μg1 ml1mg100.00 ug0.5 ml500
#27893093 // To Up
Ligation of CD40 in Human Müller Cells Induces P2X7 Receptor-Dependent Death of Retinal Endothelial Cells.Cluster of differentiation 40 (CD40) is required for retinal capillary degeneration in diabetic mice, a process mediated by the retinal endothelial cells (REC) death. However, CD40 activates prosurvival signals in endothelial cells. The purpose of this study was to identify a mechanism by which CD40 triggers programmed cell death (PCD) of RECs and address this paradox.
Jose-Andres C Portillo, Yalitza Lopez Corcino, George R Dubyak, Timothy S Kern, Shigemi Matsuyama, Carlos S Subauste
2846 related Products with: Ligation of CD40 in Human Müller Cells Induces P2X7 Receptor-Dependent Death of Retinal Endothelial Cells.1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask100 ug/vial1.00 flask1.00 flask1.00 flask
#27523466 2016/08/11 To Up
Dexamethasone reverses the effects of high glucose on human retinal endothelial cell permeability and proliferation in vitro.Diabetic macular oedema (DMO), a leading cause of preventable visual loss in the working population, is caused by an increase in microvascular endothelial cell permeability, and its prevalence is on the increase in parallel with the rising worldwide prevalence of diabetes. It is known that retinal vascular leakage in DMO is contributed to by VEGF upregulation as well as non-VEGF dependent inflammatory pathways, and the potential use of anti-inflammatory agents such as the glucocorticoids, including dexamethasone are being extensively studied. However, the mechanisms of action of dexamethasone in DMO reduction are not fully understood. Using human primary retinal endothelial cells (REC) the in vitro effect of dexamethasone in modulating the proliferation, permeability and gene expression of key tight and adheren junction components, and the expression of angiopoietins (Ang) 1 and 2 in high (25 mM) glucose conditions were investigated. High glucose decreased REC proliferation, an effect that was reversed by dexamethasone. High glucose conditions significantly increased REC permeability and decreased claudin-5, occludin and JAM-A gene expression; dexamethasone was effective in partially reversing these changes, restoring EC permeability to the normal or near normal state. High glucose levels resulted in reduction of Ang1 secretion, although Ang2 levels were consistently high. DEX increased Ang1 and decreased Ang2, indicating that the balance of Ang1/Ang2 may be important in determining functional changes in REC under high glucose conditions.
E A Stewart, S Saker, W M Amoaku
2283 related Products with: Dexamethasone reverses the effects of high glucose on human retinal endothelial cell permeability and proliferation in vitro.1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask0.5 mg
#27317266 2016/06/14 To Up
Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization.Envelope glycoprotein E2 of classical swine fever virus (CSFV) is the major antigen that induces neutralizing antibodies and confers protection against CSFV infection. There are three hypervariable antigenic regions (HAR1, HAR2 and HAR3) of E2 that are different between the group 1 vaccine C-strain and group 2 clinical isolates. This study was aimed to characterize the antigenic epitope region recognized by monoclonal antibody 4F4 (mAb-4F4) that is present in the group 2 field isolate HZ1-08, but not in the C-strain, and examine its impact on neutralization titers when antisera from different recombinant viruses were cross-examined. Indirect ELISA with C-strain E2-based chimeric proteins carrying the three HAR regions showed that the mAb-4F4 bound to HAR1 from HZ1-08 E2, but not to HAR2 or HAR3, indicating that the specific epitope is located in the HAR1 region. Of the 6 major residues differences between C-strain and field isolates, Glu713 in the HAR1 region of strain HZ1-08 is critical for mAb-4F4 binding either at the recombinant protein level or using intact recombinant viruses carrying single mutations. C-strain-based recombinant viruses carrying the most antigenic part of E2 or HAR1 from strain HZ1-08 remained non-pathogenic to pigs and induced good antibody responses. By cross-neutralization assay, we observed that the anti-C-strain serum lost most of its neutralization capacity to RecC-HZ-E2 and QZ-14 (subgroup 2.1d field isolate in 2014), and vice versa. More importantly, the RecC-HAR1 virus remained competent in neutralizing ReC-HZ-E2 and QZ-14 strains without compromising the neutralization capability to the recombinant C-strain. Thus, we propose that chimeric C-strain carrying the HAR1 region of field isolates is a good vaccine candidate for classical swine fever.
Xun Liao, Zuohuan Wang, Tong Cao, Chao Tong, Shichao Geng, Yuanxing Gu, Yingshan Zhou, Xiaoliang Li, Weihuan Fang
2164 related Products with: Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization.100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized
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