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Search results for: AntiBovine

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#30570440   2018/12/19 To Up

Detection of Alpaca Podoplanin by Immunohistochemistry Using the Antibovine Podoplanin Monoclonal Antibody PMab-44.

Podoplanin (PDPN) is expressed in type I alveolar cells of the lungs, lymphatic endothelial cells, and podocytes of the kidneys, and induces platelet aggregation through the C-type lectin-like receptor-2. PDPNs of various animal species have been characterized using specific anti-PDPN monoclonal antibodies (mAbs). However, alpaca PDPN has not previously been characterized because antialpaca PDPN mAbs have not yet been developed. In this study, we investigated the potential cross-reaction between established antibovine PDPN mAbs and alpaca PDPN. Using immunohistochemical analysis, type I alveolar cells of the alpaca lungs were detected by the antibovine PDPN mAb, PMab-44. These results indicate that PMab-44 may be useful for the detection of alpaca PDPN.
Yukinari Kato, Shinji Yamada, Shunsuke Itai, Satoru Konnai, Atsushi Kobayashi, Mika K Kaneko

1568 related Products with: Detection of Alpaca Podoplanin by Immunohistochemistry Using the Antibovine Podoplanin Monoclonal Antibody PMab-44.

100 100ug100ug100 ug1 mg100 micro gram100 ug100ug100ul

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#30570359   2018/12/20 To Up

Immunohistochemical Detection of Sheep Podoplanin Using an Antibovine Podoplanin Monoclonal Antibody PMab-44.

Podoplanin (PDPN) obtained from various animal species has been characterized using specific anti-PDPN monoclonal antibodies (mAbs), namely, PMab-1, PMab-2, PMab-32, PMab-38, PMab-44, and PMab-52 against mouse, rat, rabbit, dog, bovine, and cat PDPN, respectively. PDPN is expressed in type I alveolar cells in lungs, lymphatic endothelial cells, and kidney podocytes. In this study, we investigated possible cross-reactions between anti-PDPN mAbs and sheep PDPN. Type I alveolar cells from sheep lung were strongly detected by PMab-44 using immunohistochemical analyses. These results indicate that PMab-44 may be useful for the detection of sheep PDPN.
Yukinari Kato, Shinji Yamada, Shunsuke Itai, Atsushi Kobayashi, Satoru Konnai, Mika K Kaneko

2888 related Products with: Immunohistochemical Detection of Sheep Podoplanin Using an Antibovine Podoplanin Monoclonal Antibody PMab-44.

100ug Lyophilized100ul100ug100ul100ug100 ug100ug1 mg100 ug0.2 mg0.1 mg

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#27139791   2016/05/03 To Up

Immunohistochemical and Western Blotting Analyses of Ganoine in the Ganoid Scales of Lepisosteus oculatus: an Actinopterygian Fish.

In order to compare its characteristics with those of jaw tooth collar enamel, normally developing and experimentally regenerating ganoine from ganoid scales of Lepisosteus oculatus (spotted gar), an actinopterygian fish species, was examined by Western blotting and immunohistochemistry. Amelogenin, a major enamel matrix protein (EMP), is widely found from sarcopterygian fish to mammals. Therefore, we used antimammalian amelogenin antibodies and antisera: an antibody against bovine amelogenin; antiserum against porcine amelogenin; and region-specific antibodies or antiserum against the C-terminus, middle region, or N-terminus of porcine amelogenin in this study. Positive immunoreactivity with the antibody against bovine amelogenin, antiserum against porcine amelogenin, and the middle and C-terminal region-specific antibodies was detected in both normally developing and regenerating ganoine matrix, as well as in granules found within inner ganoine epithelial cells. These immunohistochemical analyses indicated that the Lepisosteus ganoine matrix contains EMP-like proteins with epitopes similar to mammalian amelogenins. In Western blotting analyses of regenerating ganoid scales with the antibovine amelogenin antibody, two protein bands with molecular weights of approximately 78 and 65 kDa were detected, which were similar to those found in Lepisosteus tooth enamel. Our study suggests that in Lepisosteus, EMP-like proteins in the ganoine matrix corresponded to those in tooth enamel. However, it was revealed that the 78 and 65 kDa EMP-like proteins were different from 27 kDa bovine amelogenin.
Ichiro Sasagawa, Shunya Oka, Masato Mikami, Hiroyuki Yokosuka, Mikio Ishiyama, Akane Imai, Hitoyata Shimokawa, Takashi Uchida

1189 related Products with: Immunohistochemical and Western Blotting Analyses of Ganoine in the Ganoid Scales of Lepisosteus oculatus: an Actinopterygian Fish.

100ug Lyophilized100 1 Set100 μg1 Set

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#26101772   2015/05/25 To Up

Evaluation of an Indirect-ELISA Test for Trypanosoma evansi Infection (Surra) in Buffaloes and Its Application to a Serological Survey in Thailand.

Surra, caused by Trypanosoma evansi, is a neglected disease due to frequent subclinical evolution, especially in bovines in Asia. However, acute and chronic signs are regularly observed, with significant sanitary and economic impacts. In this study, we evaluated and applied an indirect-ELISA test for the detection of anti-T. evansi immunoglobulin G in buffaloes using antibovine conjugate. Based on buffalo reference sera from the Philippines, a two-graph receiver operating characteristics analysis (TG-ROC) was conducted to define an optimal cut-off value; sensitivity and specificity were estimated at 92.5% and 94.2%, respectively. A cross-sectional serological survey was carried out in the major buffalo breeding areas of Thailand; 892 buffaloes from 8 provinces were sampled in North, Northeastern, and Southern Thailand. Seropositive buffaloes were found in all 8 provinces, on 20.3% of farms for an overall prevalence of 12.2% (95% CI 10.2-14.5%). Nearly one-third of the sampled population was exposed to infection. Broader sampling would be necessary but is not possible in the southern half-wild breeding systems. According to our results, buffaloes may constitute a large and robust reservoir for T. evansi, which is a permanent threat to other livestock such as cattle and horses as well as wild animals such as elephants in Southest Asia.
Arthur Kocher, Marc Desquesnes, Ketsarin Kamyingkird, Sarawut Yangtara, Emilye Leboucher, Pranee Rodtian, Alan Dargantes, Sathaporn Jittapalapong

1270 related Products with: Evaluation of an Indirect-ELISA Test for Trypanosoma evansi Infection (Surra) in Buffaloes and Its Application to a Serological Survey in Thailand.

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#24915569   2014/06/10 To Up

Expression of PD-L1 on canine tumor cells and enhancement of IFN-γ production from tumor-infiltrating cells by PD-L1 blockade.

Programmed death 1 (PD-1), an immunoinhibitory receptor, and programmed death ligand 1 (PD-L1), its ligand, together induce the "exhausted" status in antigen-specific lymphocytes and are thus involved in the immune evasion of tumor cells. In this study, canine PD-1 and PD-L1 were molecularly characterized, and their potential as therapeutic targets for canine tumors was discussed. The canine PD-1 and PD-L1 genes were conserved among canine breeds. Based on the sequence information obtained, the recombinant canine PD-1 and PD-L1 proteins were constructed; they were confirmed to bind each other. Antibovine PD-L1 monoclonal antibody effectively blocked the binding of recombinant PD-1 with PD-L1-expressing cells in a dose-dependent manner. Canine melanoma, mastocytoma, renal cell carcinoma, and other types of tumors examined expressed PD-L1, whereas some did not. Interestingly, anti-PD-L1 antibody treatment enhanced IFN-γ production from tumor-infiltrating cells. These results showed that the canine PD-1/PD-L1 pathway is also associated with T-cell exhaustion in canine tumors and that its blockade with antibody could be a new therapeutic strategy for canine tumors. Further investigations are needed to confirm the ability of anti-PD-L1 antibody to reactivate canine antitumor immunity in vivo, and its therapeutic potential has to be further discussed.
Naoya Maekawa, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Mami Adachi, Satoshi Takagi, Yumiko Kagawa, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi

1848 related Products with: Expression of PD-L1 on canine tumor cells and enhancement of IFN-γ production from tumor-infiltrating cells by PD-L1 blockade.

1mg10ml10100ml296T50ml25ml

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#23238318   2012/11/21 To Up

Quartz crystal microbalance immunosensor for the quantification of immunoglobulin G in bovine milk.

The development of precise and sensitive methods for milk analysis remains a challenging task in the milk quality control field. A piezoelectric immunosensor was developed for the real-time quantification of immunoglobulin G (IgG) in bovine milk and colostrum. The sensing surface was designed with rabbit antibovine IgG as the detecting molecule, coupled onto a carboxymethyl dextran-coated gold crystal. Total binding and non-specific binding were measured using a quartz crystal microbalance with dissipation (QCM-D). Conditions of analysis, including ligand immobilization, dilution ratio of milk, salinity, and pH of the dilution buffer were optimized by Doehlert experimental design in order to enhance the detection specificity. The performances of the optimized immunosensor were evaluated. The standard curve was established from QCM-D responses and was linear until an IgG concentration of 2500 ng/mL, with a detection limit of 46 ng/mL. The total assay time is 5 min per sample, including the regeneration step. The intra- and inter-assay variation coefficients were equal to or below 4.7 and 6.1%, respectively. The sensing surface was stable for 100 analyses. This technique was successfully applied to the detection of colostrum addition in milk, with a minimum threshold of 0.1%. This new IgG quantification method is particularly interesting as a cost-effective and time-saving alternative for the dairy analytical laboratories when compared with the existing quantification methods.
Cyril Crosson, Claire Rossi

2249 related Products with: Quartz crystal microbalance immunosensor for the quantification of immunoglobulin G in bovine milk.

1 mL100mg14 Arrays/Slide100 μg100 μg

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#21774472   2011/07/20 To Up

Rapid characterization of protein chips using microwave-assisted protein tryptic digestion and MALDI mass spectrometry.

We demonstrate that the microwave-assisted protein enzymatic digestion (MAPED) method can be successfully applied to the mass spectrometric characterization of proteins captured on the affinity surfaces of protein chips. The microwave-assisted on-chip tryptic digestion method was developed using a domestic microwave, completing the on-chip proteolysis reaction in minutes, whereas the previous on-chip digestion methods by incubation took hours of incubation time. For the model protein chips, antibody-presenting surfaces were prepared, where anti-α-tubulin1 and antibovine serum albumin (BSA) were immobilized on self-assembled monolayers. The resulting digestion efficiency, displaying sequence coverages of 30 and 14% for α-tubulin1 and BSA, respectively, was comparable to the previous time-consuming incubation studies. It allowed the characterization of immunosensed proteins by MASCOT search using peptide mass fingerprinting. In an example of this method for protein chip applications, BSA naturally involved in fetal bovine serum was unambiguously identified on a model protein chip by imaging mass spectrometry. This work shows that biomass spectrometry techniques can be implemented for surface mass spectrometry and biochip applications. Along with recent advances in imaging mass spectrometry, this technique will provide a new opportunity for high-speed, and thus high-throughput in the future, label-free mass spectrometric assays using protein arrays.
Na Young Ha, Shin Hye Kim, Tae Geol Lee, Sang Yun Han

1364 related Products with: Rapid characterization of protein chips using microwave-assisted protein tryptic digestion and MALDI mass spectrometry.

1000 TESTS/0.65ml100ul1 Set100ug Lyophilized10001mg10mg100ug25100 100 100ug Lyophilized

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#21678957   2011/06/30 To Up

Highly hydrophilic surfaces from polyglycidol grafts with dual antifouling and specific protein recognition properties.

Homopolymer grafts from α-tert-butoxy-ω-vinylbenzyl-polyglycidol (PGL) were prepared on gold and stainless steel (SS) substrates modified by 4-benzoyl-phenyl (BP) moieties derived from the electroreduction of the parent salt 4-benzoyl benzene diazonium tetrafluoroborate. The grafted BP aryl groups efficiently served to surface-initiate photopolymerization (SIPP) of PGL. In similar conditions, SIPP of hydroxyethyl methacrylate (HEMA) permitted the production of PHEMA grafts as model surfaces. Water contact angles were found to be 66°, 15°, and 0° for SS-BP, SS-PHEMA, and SS-PPGL, respectively. The spontaneous spreading of water drops on SS-PPGL was invariably observed with 1.5 μL water drops. PPGL thus appears as a superhydrophilic polymer. Resistance to nonspecific adsorption of proteins of PPGL and PHEMA grafts on gold was evaluated by surface plasmon resonance (SPR) using antibovine serum albumin (anti-BSA). The results conclusively show that PPGL-grafts exhibit enhanced resistance to anti-BSA adsorption compared to the well-known hydrophilic PHEMA. PPGL grafts were further modified with BSA through the carbonyldiimidazole activation of the OH groups providing immunosensing surfaces. The so-prepared PPGL-grafted BSA hybrids specifically interacted with anti-BSA in PBS as compared to antimyoglobin. It is clear that the superhydrophilic character of PPGL grafts opens new avenues for biomedical applications where surfaces with dual functionality, namely, specific protein grafting together with resistance to biofouling, are required.
Sarra Gam-Derouich, Monika Gosecka, Sandrine Lepinay, Mireille Turmine, Benjamin Carbonnier, Teresa Basinska, Stanislaw Slomkowski, Marie-Claude Millot, Ali Othmane, Dalila Ben Hassen-Chehimi, Mohamed M Chehimi

2429 related Products with: Highly hydrophilic surfaces from polyglycidol grafts with dual antifouling and specific protein recognition properties.

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#21354945   // To Up

Claudin-7-positive synchronous spontaneous intrahepatic cholangiocarcinoma, adenocarcinoma and adenomas of the gallbladder in a Bearded dragon (Pogona vitticeps).

In this study, synchronous spontaneous, independent liver and gallbladder tumours were detected in a Bearded dragon (Pogona vitticeps). The multiple tumours consisted of intrahepatic cholangiocarcinoma as well as in situ adenocarcinoma and two adenomas of the gallbladder. The biliary epithelial cells and the cholangiocarcinoma showed membranous cross-immunoreactivity for claudin-7. The gallbladder epithelial cells, its adenoma and adenocarcinoma showed basolateral cross-reactivity for claudin-7. We think that the humanised anti-claudin-7 antibody is a good marker for the detection of different primary cholangiocellular and gallbladder tumours in Bearded dragons. The cholangiocytes, the cholangiocarcinoma, the endothelial cells of the liver and the epithelial cells and gallbladder tumours all showed claudin-5 cross-reactivity. The humanised anti-cytokeratin AE1-AE3 antibody showed cross-reactivity in the biliary epithelial cells, cholangiocarcinoma cells, epithelial cells and tumour cells of the gallbladder. It seems that this humanised antibody is a useful epithelial marker for the different neoplastic lesions of epithelial cells in reptiles. The humanised anti-α-smooth muscle actin (α-SMA) antibody showed intense cross-reactivity in the smooth muscle cells of the hepatic vessels and in the muscle layer of the gallbladder. The portal myofibroblasts, the endothelial cells of the sinusoids and the stromal cells of the cholangiocarcinoma and gallbladder tumours were positive for α-SMA. The antibovine anti-vimentin and humanised anti-Ki-67 antibodies did not show crossreactivity in the different samples from the Bearded dragon.
Csaba Jakab, Miklós Rusvai, Zoltán Szabó, Péter Gálfi, Miklós Marosán, Janina Kulka, János Gál

2579 related Products with: Claudin-7-positive synchronous spontaneous intrahepatic cholangiocarcinoma, adenocarcinoma and adenomas of the gallbladder in a Bearded dragon (Pogona vitticeps).



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#21078614   2010/11/15 To Up

A multicenter pilot study to estimate the prevalence of bovine and human coagulation antibodies in the general US population.

Antibodies to bovine and human coagulation proteins have been reported to develop in some patients receiving perioperative exposure to topical bovine thrombin. To estimate the prevalence of antihuman and antibovine thrombin and factor V antibodies in the general population, this multicenter pilot study in 278 participants was undertaken. Of the participants, 88% had no detectable antibodies by enzyme-linked immunosorbent assay (ELISA). Cumulatively 22 (7.9%) of 278 of the participants were positive for at least 1 of the antibovine antibodies and only 11 (4%) of 278 were positive for human thrombin antibodies. No participants had antihuman factor V/Va antibodies. Antibodies were found in 21% of participants with no history of surgery, transfusion, or pregnancy. In participants without a surgical history, thus a low likelihood of bovine thrombin exposure, 7.9% (9 of 114) had antibovine antibodies and 3.5% (4 of 114) had human antithrombin antibodies, suggesting that antibodies may arise from contact with antigenic sources other than bovine-derived thrombin.
Jawed Fareed, Craig A Paterson, Sheila M Crean

1402 related Products with: A multicenter pilot study to estimate the prevalence of bovine and human coagulation antibodies in the general US population.

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