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Search results for: AntiCytokeratin

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#32143307   2020/03/04 To Up

Lymph Node and Bone Marrow Micrometastases Define the Prognosis of Patients with pN0 Esophageal Cancer.

Pathological routine lymph node staging is postulated to be the main oncological prognosticator in esophageal cancer (EC). However, micrometastases in lymph nodes (LNMM) and bone marrow (BNMM) are discussed as the key events in tumor recurrence. We assessed the prognostic significance of the LNMM/BNMM status in initially pN0 staged patients with curative esophagectomy.
Karl-F Karstens, Tarik Ghadban, Katharina Effenberger, Guido Sauter, Klaus Pantel, Jakob R Izbicki, Yogesh Vashist, Alexandra König, Matthias Reeh

1802 related Products with: Lymph Node and Bone Marrow Micrometastases Define the Prognosis of Patients with pN0 Esophageal Cancer.



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#30889174   2019/03/19 To Up

Detection of breast cancer lymph node metastases in frozen sections with a point-of-care low-cost microscope scanner.

Detection of lymph node metastases is essential in breast cancer diagnostics and staging, affecting treatment and prognosis. Intraoperative microscopy analysis of sentinel lymph node frozen sections is standard for detection of axillary metastases but requires access to a pathologist for sample analysis. Remote analysis of digitized samples is an alternative solution but is limited by the requirement for high-end slide scanning equipment.
Oscar Holmström, Nina Linder, Hannu Moilanen, Antti Suutala, Stig Nordling, Anders Ståhls, Mikael Lundin, Vinod Diwan, Johan Lundin

1042 related Products with: Detection of breast cancer lymph node metastases in frozen sections with a point-of-care low-cost microscope scanner.



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#30535499   2018/12/03 To Up

Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF‑α.

The purpose of this study was to evaluate the effects of concentrated growth factor exudate (CGFe) on human periodontal ligament cells (hPDLCs) stimulated by tumor necrosis factor (TNF)‑α. From the peripheral blood of healthy donors, CGFe was prepared according to the Sacco protocol after 7 days of incubation. The hPDLCs were cultured by a tissue explant method and identified with anti‑vimentin and anti‑cytokeratin antibodies. Cells were subjected to four different treatments: i) Control; ii) TNF‑α (10 ng/ml); iii) CGFe (concentration 50%); and iv) CGFe+TNF‑α. The proliferation of hPDLCs was measured with Cell Counting Kit‑8 assays. Osteogenic differentiation and mineralization were determined by Alizarin Red S staining, alkaline phosphatase activity, western blotting and reverse transcription‑quantitative polymerase chain reaction. CGFe enhanced cell proliferation and upregulated ALP activity, the mineralization level, and osteogenic‑associated osteocalcin, runt‑related transcription factor 2 and Osterix gene expression in hPDLCs under inflammatory conditions induced by TNF‑α. The present study demonstrated that CGFe enhanced hPDLC proliferation and osteogenic differentiation in the presence of TNF‑α‑induced inflammation. As CGFe can be obtained from the venous blood of patients, it generates no immune reaction. Thus, it is more economical and beneficial to use CGFe in clinical periodontal regeneration practice than synthetic growth factors.
Xiaoju Li, Huixiao Yang, Zijian Zhang, Zhonghai Yan, Huling Lv, Yan Zhang, Bin Wu

2827 related Products with: Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF‑α.

5 x 50 ug50 ug1 kit(96 Wells)1 mg10 ug4 Membranes/Box20ug100 μg10ug50 ug0.1ml (1mg/ml)100.00 ug

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#30221718   2018/09/10 To Up

Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro.

The purpose of the present study was to evaluate the effects of platelet‑rich fibrin (PRF) exudate on the proliferation, osteogenic differentiation and mineralization of human periodontal ligament cells (hPDLCs) in vitro. In the present study PRF was obtained with permission, from the peripheral blood of healthy donors and PRF exudates were collected on the 7th day of incubation. hPDLCs were obtained from healthy premolars, cultured by a tissue explant method and identified with anti‑vimentin and anti‑cytokeratin antibodies. PRF exudates were added to hPDLCs in different concentrations to evaluate cell proliferation and osteogenic differentiation. The proliferation of hPDLCs was measured using a colorimetric assay. Osteogenic differentiation and mineralization were determined by Alizarin red staining, alkaline phosphatase activity (ALP), western blotting and reverse transcription‑quantitative polymerase chain reaction. Cell proliferation was enhanced by addition of the PRF exudate, which also promoted the formation of mineralized matrix nodules and upregulated ALP activity and osteoblast‑associated levels of osteocalcin, runt‑related transcription factor and osterix gene expression. As these stimulatory effects occurred in a dose‑dependent manner, it was concluded that high concentrations of the PRF exudate served an essential role in the proliferation, osteogenic differentiation and mineralization of hPDLCs in vitro. The present study demonstrated that PRF exudate enhanced hPDLC proliferation, induced the osteoblastic differentiation of hPDLCs into mineralized tissue‑formation cells in vitro, and may therefore provide potential benefits for periodontal tissue engineering; contributing to the primary processes of periodontal tissue regeneration. From the perspective of both economics and biology, PRF has greater clinical benefits than analogous growth factors.
Xiaoju Li, Huixiao Yang, Zijiian Zhang, Zhonghai Yan, Huling Lv, Yan Zhang, Bin Wu

2207 related Products with: Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro.

5 x 50 ug50 ug101.00 flask200 100ul1.00 flask1.00 flask200 100ug Lyophilized21

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#29767232   2018/05/14 To Up

Free‑floating cancer cells in lymph node sinuses of hilar lymph node‑positive patients with non‑small cell lung cancer.

Previous studies demonstrated that free‑floating cancer cells (FFCCs) in the lymph node sinuses were of prognostic significance for colorectal and gastric cancer. The present study investigated the clinical significance of detecting FFCCs using Fast Red staining for cytokeratin in stage I/II non‑small cell lung cancer (NSCLC) patients and hilar lymph node positive NSCLC patients who underwent curative resection. Between 2002 and 2011, a total of 164 patients (including 22 hilar lymph node positive patients) were investigated. Resected lymph nodes were stained for cytokeratin using an anti‑cytokeratin antibody. In order to achieve a clear distinction from coal dust, an anti‑cytokeratin antibody was labeled with a secondary antibody conjugated with alkaline phosphatase, which was detected by a reaction with Fast Red/naphthol that produced a red color. Patients were considered to be positive for FFCCs (FFCCs+) if one or more than one free‑floating cytokeratin‑positive cell was detected in the lymph node sinuses, which could not be detected by hematoxylin and eosin staining. Among all 164 patients, a significant difference was observed in 5‑year relapse‑free survival (5Y‑RFS) rates, with 76.9 and 33.3% being achieved by FFCCs‑ and FFCCs+ patients, respectively (P<0.001). Similarly, the 5‑year overall survival (5Y‑OS) rate was significantly lower in FFCCs+ patients, with 86.6% being achieved by FFCCs‑ and 65.8% by FFCCs+ patients, respectively (P=0.014). Among 22 hilar lymph node‑positive patients, a significant difference was also observed in 5Y‑RFS, with 53.8 and 0.0% being achieved by FFCCs‑ and FFCCs+ patients, respectively (P=0.006). The 5Y‑OS tended to be lower in FFCCs+ patients, with 69.2 and 53.3% being achieved by FFCCs‑ and FFCCs+ patients, respectively (P=0.463). The findings of the present study suggested the presence of FFCCs in stage I/II NSCLC patients was associated with a poor prognosis. In addition, FFCCs in hilar lymph node‑positive patients may potential be a useful marker in foreseeing the recurrence of cancer.
Yusuke Nakamura, Masaya Mukai, Shinichiro Hiraiwa, Kyoko Kishima, Tomoko Sugiyama, Takuma Tajiri, Shunsuke Yamada, Masayuki Iwazaki

1922 related Products with: Free‑floating cancer cells in lymph node sinuses of hilar lymph node‑positive patients with non‑small cell lung cancer.



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#29305849   2018/01/03 To Up

Prognosis of Patients With Stages I and II Non-Small Cell Lung Cancer With Nodal Micrometastases.

This retrospective study aimed to determine the prognostic significance of immunohistochemically detected occult micrometastases (OM) in mediastinal lymph nodes (LNs) obtained during transcervical extended mediastinal lymphadenectomy of stages I and II non-small cell small cancer (NSCLC) patients before complete surgical resection.
Paweł Gwóźdź, Monika Pasieka-Lis, Katarzyna Kołodziej, Juliusz Pankowski, Renata Banaś, Michał Wiłkojć, Marcin Zieliński

1725 related Products with: Prognosis of Patients With Stages I and II Non-Small Cell Lung Cancer With Nodal Micrometastases.



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#28028810   2017/05/04 To Up

Three-dimensional evaluation of subclinical extension of extramammary Paget disease: visualization of the histological border and its comparison to the clinical border.

In extramammary Paget disease (EMPD), Paget cells are sometimes detected outside the clinical border (subclinical extension). However, the spreading pattern of Paget cells in subclinical extension remains unclear. In addition, the macroscopic appearances of lesions accompanied by subclinical extension are totally unknown.
T Murata, T Honda, G Egawa, A Kitoh, T Dainichi, A Otsuka, S Nakajima, S Kore-Eda, Y Kaku, S Nakamizo, Y Endo, A Fujisawa, Y Miyachi, K Kabashima

1012 related Products with: Three-dimensional evaluation of subclinical extension of extramammary Paget disease: visualization of the histological border and its comparison to the clinical border.

5 G1200 units 100 G1

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#27617283   2016/08/24 To Up

Multiple recurrent squamous cell carcinomas and utility of anticytokeratin immunohistochemistry.


Michael W Pelster, Sapna M Amin, Simon Yoo

1942 related Products with: Multiple recurrent squamous cell carcinomas and utility of anticytokeratin immunohistochemistry.

100 Tests

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#26147300   2015/07/03 To Up

Changes in the sebaceous gland in patients with male pattern hair loss (androgenic alopecia).

The sebaceous gland and the follicular bulge region have important role in biology of the hair. They initiate destruction of the hair follicle both in human and animal models in certain conditions. The morphometric feature of the sebaceous gland is not well understood so as the distribution of the bulge stem cells in pathological conditions of male pattern hair loss or androgenic alopecia (AGA).
Katsuhiro Kure, Tsukasa Isago, Takeshi Hirayama

2948 related Products with: Changes in the sebaceous gland in patients with male pattern hair loss (androgenic alopecia).

1 mg1

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#25524144   2014/12/18 To Up

Immunohistochemical expression of fibrillin-1 and fibrillin-2 during tooth development.

Oxytalan fibers are categorized as a microfibril assembly without elastin deposition, and are unique components in the periodontal ligament (PDL). However, little is known about their formation during PDL development. To clarify the mechanisms of oxytalan fiber formation in developing PDL, we performed immunohistochemical analysis to detect the direct expression of fibrillin-1 and fibrillin-2, which are major components of microfibrils.
M Kira-Tatsuoka, K Oka, E Tsuruga, M Ozaki, Y Sawa

1172 related Products with: Immunohistochemical expression of fibrillin-1 and fibrillin-2 during tooth development.

100ug250ul100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized250ML1000100ul1 mg

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