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Search results for: AntiHuman

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#34522857   2021/08/21 To Up

A resource of high-quality and versatile nanobodies for drug delivery.

Therapeutic and diagnostic efficacies of small biomolecules and chemical compounds are hampered by suboptimal pharmacokinetics. Here, we developed a repertoire of robust and high-affinity antihuman serum albumin nanobodies (Nb) that can be readily fused to small biologics for half-life extension. We characterized the thermostability, binding kinetics, and cross-species reactivity of Nbs, mapped their epitopes, and structurally resolved a tetrameric HSA-Nb complex. We parallelly determined the half-lives of a cohort of selected Nbs in an HSA mouse model by quantitative proteomics. Compared to short-lived control nanobodies, the half-lives of Nbs were drastically prolonged by 771-fold. Nbs have distinct and diverse pharmacokinetics, positively correlating with their albumin binding affinities at the endosomal pH. We then generated stable and highly bioactive Nb-cytokine fusion constructs "Duraleukin" and demonstrated Duraleukin's high preclinical efficacy for cancer treatment in a melanoma model. This high-quality and versatile Nb toolkit will help tailor drug half-life to specific medical needs.
Zhuolun Shen, Yufei Xiang, Sandra Vergara, Apeng Chen, Zhengyun Xiao, Ulises Santiago, Changzhong Jin, Zhe Sang, Jiadi Luo, Kong Chen, Dina Schneidman-Duhovny, Carlos Camacho, Guillermo Calero, Baoli Hu, Yi Shi

2962 related Products with: A resource of high-quality and versatile nanobodies for drug delivery.

200ul96 Tests2 Sample Kit10 mg250 mg400Tests

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#34513994   2021/09/01 To Up

Antitumor Effect of Saikosaponin A on Human Neuroblastoma Cells.

Neuroblastoma (NB) is a highly metastatic tumor in children that develops in the sympathetic nervous system and has a low curative rate. Saikosaponin A (SSA), an active ingredient isolated from the root of Radix Bupleuri, is a natural compound with various pharmacological activities and shows good application prospects in antitumors. This study investigated the antihuman NB activity of SSA and underlying mechanisms associated with its actions.
Tan Cheng, Muying Ying

1787 related Products with: Antitumor Effect of Saikosaponin A on Human Neuroblastoma Cells.

50 mg200 1.00 flask1mg200 0.5 ml100.00 ug0.1ml (1mg/ml)1 mL1.00 flask25 100 ug/vial

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#34307450   2021/07/09 To Up

Deep Sequencing of T-Cell Receptors for Monitoring Peripheral CD8 T Cells in Chinese Advanced Non-Small-Cell Lung Cancer Patients Treated With the Anti-PD-L1 Antibody.

Atezolizumab, a high-affinity engineered human anti-PD-L1 antibody, has produced a clinical benefit for patients with advanced non-small-cell lung cancer (NSCLC). However, associated with T-cell regulation, the immunomodulatory effect of PD-L1 blockade and its biomarker in peripheral immunity remains elusive. In a prospective cohort with 12 Chinese advanced NSCLC patients who received atezolizumab 1,200 mg every 3 weeks as a second-line treatment, blood samples were obtained before and 6 weeks after atezolizumab initiation, and when disease progression was confirmed. Patients were classified into a response or progression group according to response evaluation criteria in solid tumors (RECIST) 1.1. Fresh peripheral blood mononuclear cells (PBMCs) from patients were stained with antihuman CD3, CD8, and PD-1 antibodies for flow cytometry analysis. T-cell receptor (TCR)-β chains of CD8 T cells were analyzed by next-generation sequencing (NGS) at the deep level. Diversity, clonality, and similarity of TCR have been calculated before and after treatment in both groups. Clonal expansion with high PD-1 expression was detected in all patients' peripheral CD8 T cells before the treatment of atezolizumab. Unlike the progression group, the diversity of TCR repertoire and singletons in the TCRβ pool increased over time with atezolizumab administration, and the TCR repertoire dynamically changes in the response group. The percentage of CD8 PD-1 terminal exhausted T cells declined in the response group after the PD-L1 blockade. Two patterns of TCR changes among patients who received PD-L1-targeted immunotherapy were observed. Deep sequencing of the T-cell receptors confirmed the existence of CD8 PD-1high T cells with an exhaustion phenotype in Chinese NSCLC patients. Our study demonstrated that efficient anti-PD-L1 therapy could reshape the TCR repertoire for antitumor patients. Furthermore, singleton frequency may help us select patients who are sensitive to anti-PD-L1 immunotherapy.
Jin Sheng, Huadi Wang, Xiao Liu, Yunyun Deng, Yingying Yu, Pengfei Xu, Jiawei Shou, Hong Pan, Hongsen Li, Xiaoyun Zhou, Weidong Han, Tao Sun, Hongming Pan, Yong Fang

2915 related Products with: Deep Sequencing of T-Cell Receptors for Monitoring Peripheral CD8 T Cells in Chinese Advanced Non-Small-Cell Lung Cancer Patients Treated With the Anti-PD-L1 Antibody.

1mg

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#34285339   2021/07/20 To Up

Functional genomics for breast cancer drug target discovery.

Breast cancer is a heterogeneous disease that develops through a multistep process via the accumulation of genetic/epigenetic alterations in various cancer-related genes. Current treatment options for breast cancer patients include surgery, radiotherapy, and chemotherapy including conventional cytotoxic and molecular-targeted anticancer drugs for each intrinsic subtype, such as endocrine therapy and antihuman epidermal growth factor receptor 2 (HER2) therapy. However, these therapies often fail to prevent recurrence and metastasis due to resistance. Overall, understanding the molecular mechanisms of breast carcinogenesis and progression will help to establish therapeutic modalities to improve treatment. The recent development of comprehensive omics technologies has led to the discovery of driver genes, including oncogenes and tumor-suppressor genes, contributing to the development of molecular-targeted anticancer drugs. Here, we review the development of anticancer drugs targeting cancer-specific functional therapeutic targets, namely, MELK (maternal embryonic leucine zipper kinase), TOPK (T-lymphokine-activated killer cell-originated protein kinase), and BIG3 (brefeldin A-inhibited guanine nucleotide-exchange protein 3), as identified through comprehensive breast cancer transcriptomics.
Tetsuro Yoshimaru, Yusuke Nakamura, Toyomasa Katagiri

2729 related Products with: Functional genomics for breast cancer drug target discovery.

100ul250 ml

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#34283639   2021/07/19 To Up

Epitope Mapping of an Antihuman EGFR Monoclonal Antibody (EMab-134) Using the REMAP Method.

The epidermal growth factor receptor (EGFR) is a tyrosine kinase receptor that plays an important role in normal epidermal cell physiology. EGFR is overexpressed in cancer cells and has a number of mutations that implicate tumor malignancy, development, and poor patient prognosis; thus, EGFR is an attractive target for cancer therapy. At present, anti-EGFR monoclonal antibodies (mAbs) have been approved and are used for treating patients with a variety of EGFR-expressing cancers. Epitope mapping is important in identifying the therapeutic mechanism of anti-EGFR mAbs; however, the development of epitope mapping techniques lags behind the development of antimolecular target mAbs, including anti-EGFR mAbs. Hence, in this study, a novel epitope mapping method, RIEDL insertion for epitope mapping (REMAP) method, was developed. The results of this study demonstrated that the critical epitope of anti-EGFR mAb EMab-134 is Gly378, Asp379, Ser380, Phe381, Thr382, His383, Thr384, Pro385, and Pro386 of EGFR. The REMAP method could be useful for determining the critical epitope of functional mAbs against many target molecules.
Masato Sano, Mika K Kaneko, Teizo Aasano, Yukinari Kato

1307 related Products with: Epitope Mapping of an Antihuman EGFR Monoclonal Antibody (EMab-134) Using the REMAP Method.

100ug Lyophilized1 mg1 mg100ul100ug Lyophilized0.25 mg1 mg200 ug0.1 mg100ug

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#34173367   2021/06/22 To Up

Filaggrin Expression in the Lid Margin During Contact Lens Wear.

To investigate the expression of the keratinization-related protein, filaggrin, in the lid margin epithelium of contact lens (CL) wearers compared with nonwearers.
Waleed M Alghamdi, Maria Markoulli, Eric B Papas

2077 related Products with: Filaggrin Expression in the Lid Margin During Contact Lens Wear.

300 units1100 units

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#34170640   // To Up

Anti-ALe: a mind boggler.

The Lewis blood group system is unique because antigens are neither alleles of the same gene nor are they synthesized by red blood cells (RBCs); rather, they are adsorbed onto the RBC membrane from plasma as glycolipids. Antibodies against Lewis antigens are predominantly naturally occurring immunoglobulin (Ig)M type that sometimes react at 37°C and the antihuman globulin phase. Lewis compound antigens, ALe and BLe, have been described that were confirmed because of the presence of antibodies against them. These compound antigens are the result of an interaction between , and genes.
A Gupta, K Chaudhary, S Asati, B Kakkar

1091 related Products with: Anti-ALe: a mind boggler.

50 ug100μl100ug Lyophilized100ug Lyophilized500 100 μg100ug Lyophilized100 µg0.1 mg100ug100ug413 μg

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#34150714   2021/06/02 To Up

Oriented Antibody Covalent Immobilization for Label-Free Impedimetric Detection of C-Reactive Protein Direct and Sandwich Immunoassays.

The detection and monitoring of biological markers as disease indicators in a simple manner is a subject of international interest. In this work, we report two simple and sensitive label-free impedimetric immunoassays for the detection of C-reactive protein (CRP). The gold electrode modified with boronic acid-terminated self-assembled monolayers afforded oriented immobilization of capture glycosylated antibody (antihuman CRP monoclonal antibody, mAb). This antibody-modified surface was able to capture human CRP protein, and the impedance signal showed linear dependence with CRP concentration. We confirmed the immobilization of anti-CRP mAb using surface sensitive X-ray photoelectron spectroscopy (XPS) and electrochemical impedance. The oriented covalent immobilization of mAb was achieved using glycosylated Fc (fragment, crystallizable) region specific to boronic acid. The direct immunoassay exhibited a linear curve for concentration range up to 100 ng ml. The limit of detection (LoD) of 2.9 ng ml, limit of quantification (LoQ) of 9.66 ng ml, and sensitivity of 0.585 kΩ ng ml cm were obtained. The sandwich immunoassay was carried out by capturing polyclonal anti-CRP antibody (pAb) onto the CRP antigen immunoreaction. The impedance signal after pAb capture also showed linear dependence with CRP antigen concentration and acted as a CRP antigen detection signal amplifier. The detection of the CRP antigen using sandwich pAb immunoassay improved LoD to 1.2 ng ml, LoQ to 3.97 ng ml, and enhanced the sensitivity to 0.885 kΩ ng ml cm. The real sample analysis, using newborn calf serum, showed excellent selectivity and % recovery for the human CRP ranging from 91.2 to 96.5%. The method was reproducible to 4.5% for direct immunoassay and 2.3% for sandwich immunoassay.
Abiola Adesina, Philani Mashazi

1827 related Products with: Oriented Antibody Covalent Immobilization for Label-Free Impedimetric Detection of C-Reactive Protein Direct and Sandwich Immunoassays.

20 ml1 mg1mg2 mg 200ul100 ul1 mg1mg100ul1 mg100

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#34146864   2021/06/17 To Up

Crystalline appearance in light chain cast nephropathy is associated with higher early mortality in patients with newly diagnosed multiple myeloma.

Light chain cast nephropathy (LCCN) is the most common kidney lesion in multiple myeloma patients. LCCN may exhibit a crystalline appearance. The frequency and clinical significance of crystalline LCCN are not well understood. Here, we report the first retrospective study of crystalline LCCN.
Zi-Shan Lin, Xu Zhang, Xiao-Juan Yu, Shuang Wang, Su-Xia Wang, Yu-Jun Dong, Fu-De Zhou, Ming-Hui Zhao

1975 related Products with: Crystalline appearance in light chain cast nephropathy is associated with higher early mortality in patients with newly diagnosed multiple myeloma.

25 mg5 mg5 mg1 mg1 mg5 mg1 mg 0.1 mg 5 mg16 Arrays/Slide

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#34028385   2021/05/19 To Up

Stability of Antihuman Leukocyte Antigen Sensitization Profiles in Highly Sensitized Kidney Transplantation Candidates: Towards a Rational Serological Testing Strategy.

Highly sensitized (HS) anti-human leukocyte antigens (HLA) patients awaiting kidney transplantation benefit from specific allocation programs. Serological monitoring at 3-month intervals is recommended to prevent unexpected positive crossmatch (XM), but this strategy is not evidence-based. Therefore, we assessed its relevance when using single-antigen flow bead (SAFB) and screening flow bead (SFB) assays.
Elodie Wojciechowski, Frédéric Jambon, Marine Cargou, Gwendaline Guidicelli, Pierre Merville, Lionel Couzi, Jean-Luc Taupin, Jonathan Visentin

1567 related Products with: Stability of Antihuman Leukocyte Antigen Sensitization Profiles in Highly Sensitized Kidney Transplantation Candidates: Towards a Rational Serological Testing Strategy.

4 Arrays/Slide2 Pieces/Box4 Membranes/Box2 Pieces/Box4 Membranes/Box2 Pieces/Box0.1ml4 Arrays/Slide2 Pieces/Box

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