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Search results for: AntiRat

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#28339303   2017/03/24 To Up

Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.
Yuki Fujii, Mika K Kaneko, Satoshi Ogasawara, Shinji Yamada, Miyuki Yanaka, Takuro Nakamura, Noriko Saidoh, Kanae Yoshida, Ryusuke Honma, Yukinari Kato

1428 related Products with: Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

1l25ml 0.1 mg25ml5ml25ml 0.1 mg1ml10ml100ml250mg

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#24093638   // To Up

Bronchus-associated lymphoid tissue in pulmonary hypertension produces pathologic autoantibodies.

Autoimmunity has long been associated with pulmonary hypertension. Bronchus-associated lymphoid tissue plays important roles in antigen sampling and self-tolerance during infection and inflammation.
Kelley L Colvin, Patrick J Cripe, D Dunbar Ivy, Kurt R Stenmark, Michael E Yeager

2712 related Products with: Bronchus-associated lymphoid tissue in pulmonary hypertension produces pathologic autoantibodies.



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#22348528   2012/03/05 To Up

The use of melatonin in hypoxic-ischemic brain damage: an experimental study.

Oxidative stress (OS) plays a key role in perinatal brain damage. The aim of this study is to evaluate the effectiveness of melatonin as a neuroprotective drug by investigating the influence of melatonin on OS and inflammation biomarkers in an animal model of cerebral hypoxia-ischemia.
W Balduini, S Carloni, S Perrone, S Bertrando, M L Tataranno, S Negro, F Proietti, M Longini, G Buonocore

2450 related Products with: The use of melatonin in hypoxic-ischemic brain damage: an experimental study.

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#21629060   // To Up

Role of anti-TNF-α therapy in fat graft preservation.

In this study, we evaluated the role of antitumor necrosis factor-alpha (TNF-α) therapy in the decrease of adipocyte apoptosis and weight preservation of fat grafts in the rat model.
Mei Yang, Feng Zhang, Frederick C Sailes, Elizabeth W Zhang, Shuyin Lin, Suman K Das

2951 related Products with: Role of anti-TNF-α therapy in fat graft preservation.

100 UG100 μg100ug100 μg100 μg100ug100ug100ug0.1ml (1mg/ml)

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#19799436   2009/10/02 To Up

Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.

Closely involved in the progression of nonlinear bioimaging is the development of optical probes for investigating biological function and activity. Introduction of new fluorescent compounds possessing enhanced nonlinearities is essential for advancing the utility of two-photon absorption (2PA) processes in the biological sciences. Herein, we report the synthesis of fluorene-based fluorophores tailored for multiphoton imaging, incorporating the succinimidyl ester and thioester functionality as reactive linkers for further coupling with a wide variety of biologically relevant molecules. The succinimidyl ester amine reactive probe was conjugated with the cyclic peptide RGDfK and polyclonal antirat IgG protein. Upon conjugation, the basic molecular architecture and photophysical properties of the active 2PA chromophore remain unchanged. Conventional and two-photon fluorescence microscopy (2PFM) imaging of COS-7 and HeLa cells, incubated with either the fluorene-RGD peptide conjugate or the fluorene-IgG conjugate, was demonstrated. The fluorene-IgG conjugate was used to image cell spindles at early mitotic developmental stages.
Alma R Morales, Ciceron O Yanez, Katherine J Schafer-Hales, Adam I Marcus, Kevin D Belfield

1673 related Products with: Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.

10reactions 10reactions 10reactions (20 µl each)10reactions 10reactions

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#19301799   // To Up

Fate of mu receptors during rat skeletogenesis.

Studies have intimated a role for endogenous opioids in skeletal ontogeny. We hypothesized that this role may be confined to the perinatal period. We, therefore, examined both fetal and postnatal rat long bones to determine the pattern, if any, of mu receptor expression. Perfused long bones and brains were harvested from 3 to 4 each of near-term fetuses, 3- to 4-week-old neonates, and 6-week-old juveniles. Tissues were decalcified, embedded, sectioned, quenched of peroxidase, and then blocked. Sections were incubated overnight with either rabbit antirat mu receptor IgG or naïve rabbit IgG (control) at 4 degrees C. The next day, sections were washed, blocked again, and incubated with biotin-labeled secondary antibody, streptavidin-peroxidase conjugate, and a chromogen substrate with intervening wash steps. Slides were counterstained with hematoxylin and coverslipped. Digital photomicrographs were then imported into an image analysis program and the percent area of stained cortical and trabecular bone quantified. Mu receptor expression decreased significantly with age. Approximately 25% of the area of fetal long bones stained positively, including the endosteum, periosteum, and the growth plate. Little or no nonspecific staining occurred. Staining in neonatal tissue was diminished to <11% of the area and involved areas of apparent remodeling; chondrocytes in the growth plates failed to stain. Finally, juvenile bone evidenced staining approaching background levels produced by control slides (approximately 2%). Mu receptors are abundant in developing rat long bones during fetal development but become progressively less abundant postnatally. This infers a role for endogenous opioids during skeletal ontogeny.
Timothy E Cooney, Elisa M Konieczko, Lindsey Roach, Bradley Poole

2661 related Products with: Fate of mu receptors during rat skeletogenesis.

125 ml 1 mg1 mg 100ug Lyophilized100 μg1 mg0.5mg100ug

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#18477215   // To Up

New rat model induced by anti-glomerular basement membrane antibody shows severe glomerular adhesion in early stage and quickly progresses to end-stage renal failure.

The aim of the present study was to introduce a new anti-glomerular basement membrane nephritis model in which plasma creatinine levels dramatically increased only 4 weeks after a single administration of rabbit antirat glomerular basement membrane antibody in Sprague-Dawley rats. According to renal morphology, glomerular lesions characterized by mesangial expansion and adhesion of the glomerular tuft to Bowman's capsule were observed in the early stage at day 7 after disease induction; adhesion was detected in approximately 90% of glomeruli 14 days after antibody injection. After 21 days the rats exhibited pronounced glomerulosclerosis/hyalinosis and severe tubulointerstitial lesions characterized by interstitial fibrosis. Urinary podocytes excreted in nephritis rats were studied and it was found that urinary podocyte loss might be closely related to progression of renal injury. Because this new model simply and reproducibly demonstrates development of end-stage renal disease, it will be beneficial for elucidating mechanisms by which chronic renal injury irreversibly progresses, as well as for developing therapeutic agents for chronic renal failure.
Yoshimasa Nakano, Takahiro Hirano, Kenji Uehara, Seiji Nishibayashi, Katsuji Hattori, Miki Aihara, Yoshihisa Yamada

2184 related Products with: New rat model induced by anti-glomerular basement membrane antibody shows severe glomerular adhesion in early stage and quickly progresses to end-stage renal failure.

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#18201058   // To Up

Purification and localization of nitric oxide synthases from hybrid tilapia (Nile tilapia x Mozambique tilapia).

The aims of this study were to purify and localize the nitric oxide synthases (NOSs) from hybrid tilapia (Nile tilapia Oreochromis niloticus x Mozambique tilapia O. mossambicus). The purification procedures involved affinity chromatography with a 2', 5'-ADP-agarose 4B column and ion exchange with a diethylaminoethanol Bio-Gel A column. The results from gel filtration assays showed that the molecular weights of neuronal NOS (nNOS) and inducible NOS (iNOS) were 178 and 120 kDa, respectively. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis results showed that there were three bands with molecular weights of 89, 47, and 29 kDa from the purified nNOS. However, only one band, with a molecular weight of 120 kDa, appeared on the gel from the purified iNOS. Hybrid tilapia nNOS was a dimer structure, while iNOS appeared to be a monomer structure. Moreover, our results revealed that the activities of nNOS and iNOS were significantly higher after the addition of Ca+2 or Mg+2 ions individually. However, when L-arginine and NADPH were present, the addition of 1 mM of either ion did not further increase the activity. The chemical L-N(G)-methyl-L-arginine could inhibit the activities of the purified NOSs with or without L-arginine. Western blot analyses showed only an 89-kDa immunoreactive band from the extracts of cerebrum; however, we did not find the specific bands in other tissues, such as gill, intestine, liver, spleen, and anterior kidney. We found another 120-kDa immunoreactive protein band with the rabbit antirat iNOS serum against iNOS from the extracts of anterior kidney and spleen. The results of immunohistochemistry with the rabbit antihuman nNOS serum indicated that the nNOS existed in the cerebellum, olfactory bulb, diencephalons, and nerve cell bodies and neuronal fibers of the spinal cord. Interestingly, only macrophages from anterior kidney and spleen showed positive reactions with the rabbit antirat iNOS serum. In the same way, the endothelial NOS (eNOS) located in the heart and epithelial cells of the blood vessels reacted positively with the rabbit antibovine eNOS serum.
Way-Shyan Wang, Shao-Wen Hung, Yu-Hsing Lin, Ching-Yu Tu, Min-Liang Wong, Shiow-Her Chiou, Meng-Tong Shieh

1291 related Products with: Purification and localization of nitric oxide synthases from hybrid tilapia (Nile tilapia x Mozambique tilapia).

100100100 200 assays96tests2 x 96 assays100 1100 2 x 96 well plate100Tests250ml

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