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Memory B Cell Activation, Broad Anti-influenza Antibodies, and Bystander Activation Revealed by Single-Cell Transcriptomics.

Antibody memory protects humans from many diseases. Protective antibody memory responses require activation of transcriptional programs, cell proliferation, and production of antigen-specific antibodies, but how these aspects of the response are coordinated is poorly understood. We profile the molecular and cellular features of the antibody response to influenza vaccination by integrating single-cell transcriptomics, longitudinal antibody repertoire sequencing, and antibody binding measurements. Single-cell transcriptional profiling reveals a program of memory B cell activation characterized by CD11c and T-bet expression associated with clonal expansion and differentiation toward effector function. Vaccination elicits an antibody clone, which rapidly acquired broad high-affinity hemagglutinin binding during affinity maturation. Unexpectedly, many antibody clones elicited by vaccination do not bind vaccine, demonstrating non-specific activation of bystander antibodies by influenza vaccination. These results offer insight into how molecular recognition, transcriptional programs, and clonal proliferation are coordinated in the human B cell repertoire during memory recall.

2019 related Products with: Memory B Cell Activation, Broad Anti-influenza Antibodies, and Bystander Activation Revealed by Single-Cell Transcriptomics.

Mouse Anti-Human CD18 (Ac Rabbit Anti-Human B-cell Rabbit Anti-Human Red Blo Anti bodywall muscle cell Mouse Anti-Human Prolifer Mouse Anti-Human Endothel Anti-bodywall muscle cell Rat monoclonal anti mouse Rabbit Anti-Rat Androgen Rabbit Anti-Cell death in Rabbit Anti-cellulase Pol Anti BTG1(B cell transloc

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Lack of association between amino acid sequences of the bovine leukemia virus envelope and varying stages of infection in dairy cattle.

We collected 724 blood samples from dairy cattle from six Mexican states, and tested them for the presence of antibodies against BLV using a commercial ELISA test. Our study groups consisted of 32 samples: 12 asymptomatic cows, 12 cows with lymphocytosis and 8 samples of tumor tissue of the abomasum and heart of cattle with lymphoma. We designed three pairs of primers to amplify the complete BLV env gene, and obtained a fragment of 1548 nucleotides in length with the sequenced products. According to the phylogenetic tree we constructed to identify the viral genotype, 96.87% of the sequences grouped into genotype 1, while a single sample from a cow with lymphocytosis (3.13%) was associated with genotype 3 sequences. The similarity between the Mexican BLV sequences ranged from 0.985 to 1.00. In addition, the proportion of non-synonymous and synonymous mutations indicated negative selection. We did not identify any conserved residues in the viral protein sequences that could be related to BLV infection stage in cattle. Proviral quantification was performed using quantitative polymerase chain reaction, and we used Mood´s median test as statistical analysis. We found no significant association between proviral load and phase of infection. The sequences showed high similarity without any association between BLV surface glycoprotein and the different infection stages, nor differences in the proviral load. BLV genotype 1 was identified as prevalent in the studied samples, and for the first time in Mexico, we identified BLV genotype 3 in cattle.

2123 related Products with: Lack of association between amino acid sequences of the bovine leukemia virus envelope and varying stages of infection in dairy cattle.

Ofloxacin CAS Number [824 3-Amino-1,4-dimethyl-5H-p (2S,4S)-4-Amino-1,2-pyrro 7-Aminoheptanoic Acid Met Avian Influenza virus H5N 2 Amino 6 fluorobenzoic a EDANS sodium salt [5 ((2 5-Amino-2-naphthalenesulf N-[2-[4-(Aminosulfonyl)ph 5 (2 Aminoethylamino) 1 n Native Parainfluenza Viru L-α-Aminoxy-β-phenylpro

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Immunohistochemical analysis of cancer-associated fibroblasts and podoplanin in head and neck cancer.

To immunohistochemically evaluate the association between the presence of cancer-associated fibroblasts (CAFs) and the tumour expression of podoplanin (PDPN) in head and neck squamous cell carcinoma (HNSCC) and their association with clinicopathological variables.

1085 related Products with: Immunohistochemical analysis of cancer-associated fibroblasts and podoplanin in head and neck cancer.

Head & Neck cancer tissue Head and neck cancer tiss Head and neck squamous ca Multiple head and neck ca Head & Neck cancer test t Head and neck cancer tiss Multiple head and neck ca Head & Neck cancer tissue Cancer samples: Head and Head & Neck cancer test t Multiple Head & Neck canc Cancer Samples: Head and

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Significant cardiac disease complicating Graves' disease in previously healthy young adults.

Graves' disease is associated with tachydysrythmia, cardiac ischaemia and cardiomyopathy - all uncommon in young adults without previous cardiac disease. We present three young individuals who developed cardiac complications after periods of uncontrolled Graves' disease. Subject 1: A 34-year-old female had severe thyrotoxic symptoms for weeks. Investigations showed fT4: 98.4 (11-25 pmol/L), fT3: 46.9 (3.1-6.8 pmol/L), TSH <0.01 (0.27-4.2 mU/L) and thyrotrophin receptor antibody (TRAb): 34.8 (<0.9 U//l). She had appropriate treatment but several weeks later she became breathless despite improving thyroid function. Echocardiography showed a pericardial effusion of 2.9 cm. She responded well to steroids and NSAIDs but developed active severe Graves' orbitopathy after early total thyroidectomy. Subject 2: A 28-year-old male developed thyrotoxic symptoms (fT4: 38 pmol/L, fT3: 13.9 pmol/L, TSH <0.01 (for over 6 months) and TRAb: 9.3 U/L). One month after starting carbimazole, he developed acute heart failure (HF) due to severe dilated cardiomyopathy - EF 10-15%. He partially recovered after treatment - EF 28% and had early radioiodine treatment. Subject 3: A 42-year-old woman who had been thyrotoxic for several months (fT4: 54.3; fT3 >46.1; TSH <0.01; TRAb: 4.5) developed atrial fibrillation (AF) and heart failure. Echocardiography showed cardiomegaly - EF 29%. She maintains sinus rhythm following early total thyroidectomy (EF 50%). Significant cardiac complications may occur in previously fit young adults, who have had uncontrolled Graves' disease for weeks to months. Cardiac function recovers in the majority, but early definitive treatment should be discussed to avoid Graves' disease relapse and further cardiac decompensation.

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HIV Self Test Kit, 1Test Prostate disease spectrum Infection diseases: Heli Lung disease spectrum tis Brain disease spectrum (b Beta Amyloid (1 40) ELISA rHIV gp41, soluble Antige HBV-5 panel test, sAg sAb Ovary disease spectrum (o Multiple organ diseased t Malaria pf antigen test, Colon disease spectrum ti

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First experimental proof of Rotavirus A (RVA) genotype G18P[17] inducing the clinical presentation of 'young pigeon disease syndrome' (YPDS) in domestic pigeons (Columba livia).

Young pigeon disease syndrome (YPDS) is characterized as a seasonally occurring, acute and primarily enteric medical condition of mainly juvenile domestic pigeons (Columba livia) with highly variable mortality reaching more than 50%. Although the syndrome has been known in Europe for almost three decades, its aetiology remains largely obscure. Recently, a previously unknown pigeon-associated clade of Rotavirus A (RVA) genotype G18P[17] was detected in Europe and Australia in association with fatal diseases resembling YPDS. Here we show for the first time, that peroral inoculation of healthy juvenile homing pigeons with two genetically different cell culture isolates of RVA G18P[17] (106.3 foci-forming units per bird) induces an acute and self-limiting YPDS-like disease in all infected birds. Clinical signs included regurgitation, diarrhoea, congested crops, anorexia and weight loss, as described for naturally RVA-infected pigeons. In agreement with the original outbreaks, RVA isolate DR-7 induced more pronounced clinical signs as compared to isolate DR-5, indicating strain-dependent virulence factors to contribute to variable disease outcomes observed in the field. All inoculated birds developed rotavirus-reactive antibodies starting at seven days after inoculation. High levels of viral RNA and infectious virus were detectable in cloacal swabs and faecal samples already three days after inoculation. While shedding of infectious virus subsided within few days, moderate viral RNA levels were still detectable in cloacal swabs, faeces and tissue samples at the end of the experiment three weeks after inoculation. Histopathological analysis at this time point revealed inflammatory lesions in spleens and livers of pigeons from both infected groups. In summary, we fulfilled Henle-Koch´s postulates and confirmed RVA G18P[17] as a primary cause of YPDS-like diseases in domestic pigeons. By establishing an infection model, we provide a crucial tool for future research, such as identification of transmission routes and establishing vaccination regimes.

2654 related Products with: First experimental proof of Rotavirus A (RVA) genotype G18P[17] inducing the clinical presentation of 'young pigeon disease syndrome' (YPDS) in domestic pigeons (Columba livia).

Skin disease tissue array Lung disease spectrum tis Multiple organ tumor tiss Breast disease spectrum t Ovary disease spectrum (o Liver disease spectrum ti Tissue array of gastric d Male genitourinary system Ovarian disease spectrum Colon disease spectrum ti Multiple diseases of live Colon cancer tissue array

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A novel approach enables imaged capillary isoelectric focusing analysis of PEGylated proteins.

PEGylation has been used as a strategy to enhance pharmacokinetic properties of therapeutic proteins by pharmaceutical industry. Imaged capillary isoelectric focusing (icIEF) is the current industry standard technology for isoelectric point (pI) determination and charge variant quantification of proteins and antibodies. However, the charge variants of PEGylated proteins merge into one broad peak during icIEF, most likely due to masking of proteins by the surrounding polyethylene glycol chain as well as the increased hydrodynamic volume due to PEGylation. Here we report our novel matrix formula with a combination of glycine and taurine (GLY-T) that significantly improved the separation of charge variants in PEGylated proteins. As a result, it is no longer necessary to conduct isoelectric focusing of proteins prior to PEGylation, which does not reflect the changes caused by PEGylation and purification processes. The novel matrix (GLY-T) enables icIEF analysis of PEGylated proteins in their real conjugated states. This article is protected by copyright. All rights reserved.

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MarkerGeneTM Hydrophobic Recombinant Chicken GH An Recombinant Human IL-32 a Recombinant Human GRO-alp Proteins and Antibodies H Proteins and Antibodies H Recombinant Human ACTG1 P Recombinant Human FGF1 FG Recombinant Human AACT SE Native Bovine ALPI CIAP P Recombinant Rat IL-1 alph Recombinant HCV NS-4a+b A

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Engineered scPDL1-DM1 drug conjugate with improved in vitro analysis to target PD-L1 positive cancer cells and intracellular trafficking studies in cancer therapy.

Antibody-drug conjugates (ADC), precisely deliver a cytotoxic agent to antigen-expressing tumor cells by using specific binding strategies of antibodies. The ADC has shown the ability of potent bio-therapeutics development but indefinite stoichiometric linkage and full-length antibody penetration compromised the field of its advancement. Single chain variable fragments convention instead of the full-length antibody may overcome the challenge of rapid penetration and internalization. Programmed cell death ligand-1 interaction with PD-1 has recently revolutionized the field of immunotherapy. We systematically designed scPDL1-DM1 drug conjugate by linking scFv-PD-L1 proteins (scFv) with maytansinoids (DM1) cytotoxic agent through succinimidyl trans-4-maleimidylmethyl cyclohexane-1- carboxylate (SMCC) linker. Binding affinity was confirmed by immunocytochemistry, spectrophotometry and gel electrophoresis analysis. The scPDL1-DM1 showed specific binding with PD-L1 positive tumor cells and retained in vitro anti-cell proliferation activity. The intracellular trafficking of the drug was evaluated in A549 cancer cell lines, and maximum trafficking was observed after two hours of incubation. The generated drug can be utilized as a potent tool for site-specific conjugation, predicting specificity in vitro activities with extended range against PD-L1 positive cancer cells and can be utilized for further in vivo testing and clinical therapeutics development.

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Head & Neck cancer test t Oral squamous cell cancer Bladder cancer tissue arr Middle advanced stage bre Colon cancer test tissue Esophageal cancer tissue Thymus cancer test tissue Liver cancer antibody scr Stomach cancer tissue arr Cervix cancer survey tiss Kidney cancer test tissue Cancer Apoptosis Phospho-

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Using NS1 flavivirus protein microarray to infer past infecting dengue virus serotype and number of past dengue virus infections in Vietnamese individuals.

The use of multiplex microarray assays, in which antibodies are measured against multiple related antigens, is gaining increased focus for use in seroepidemiological studies to infer past transmission. We assess the performance of a flavivirus microarray assay for determining past dengue virus infection history in a dengue-endemic setting, Vietnam. We tested the microarray on samples from 1 and 6 months post-infection from DENV-infected patients (infecting serotype determined using RT-PCR during acute infection, past primary and secondary infection assessed using PRNT 6 months post-infection). Binomial models developed to discriminate past primary from secondary infection using the PMA titres had high AUC (0.90-0.97) and accuracy (0.84-0.86). Multinomial models developed to identify the most recent past infecting serotype using PMA titres performed well in those with past primary (average test-set κ=0.85, accuracy=0.92), but not those with past secondary infection (κ=0.24, accuracy=0.45). Our results suggests that the microarray will be useful in sero-epidemiological studies aimed at classifying the past infection history of individuals (past primary vs secondary and serotype of past primary infections) and thus inferring past transmission intensity of dengue virus in dengue-endemic settings. Future work to validate these models should be undertaken in different transmission settings and with samples later after infection.

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Recombinant Dengue Virus FIV Core Ag, recombinant Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus

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Seroprevalence of varicella antibodies in adults without clinical history of disease.

Varicella in adults and immunocompromised patients can be severe. The clinical diagnosis of varicella has high accuracy and the history of disease has a high positive predictive value for protection. A significant portion of adults, however, cannot remember if they have had varicella, especially older individuals. We conducted a cross-sectional study to determine the seroprevalence of varicella protective antibodies titers in adults with no clinical history of disease, attended at a Reference Center for Special Immunobiologicals and Travel Medicine in Rio de Janeiro (Brazil). Titration of immunoglobulin G (IgG) antibodies to varicella-zoster was determined by chemiluminescence immunoassay. Among 140 adults without history of varicella, 92% had protective antibody titers. We concluded that seroprevalence of varicella-zoster protection was very high in adults with negative history of disease and the use of serology before vaccination reduced significantly unnecessary vaccine and immunoglobulin use.

1930 related Products with: Seroprevalence of varicella antibodies in adults without clinical history of disease.

Ovary disease spectrum (o Skin disease tissue array Breast disease spectrum t Male genitourinary system Liver disease spectrum ti Ovarian disease spectrum Colon disease spectrum ti Multiple organ tumor tiss Lung disease spectrum tis Goat Anti-Human APOA4, (i Goat Anti-Human GOT2 (aa Stomach cancer tissue arr

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