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Search results for: Antibodies

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#35045376   2022/01/13 To Up

Identification of anti-lipoarabinomannan antibodies against mannan core and their effects on phagocytosis of mycobacteria by human neutrophils.

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Hitoshi Nakayama, Eriko Oshima, Tomomi Hotta, Kei Hanafusa, Kota Nakamura, Noriko Yokoyama, Hideoki Ogawa, Kenji Takamori, Kazuhisa Iwabuchi

2942 related Products with: Identification of anti-lipoarabinomannan antibodies against mannan core and their effects on phagocytosis of mycobacteria by human neutrophils.

5000.1 mg1 ml200 TESTS50 100.00 ug1mg100 μg0.1 mg

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#35045362   2022/01/16 To Up

Safety and immunogenicity of a measles-vectored SARS-CoV-2 vaccine candidate, V591 / TMV-083, in healthy adults: results of a randomized, placebo-controlled Phase I study.

V591 (TMV-083) is a live recombinant measles vector-based vaccine candidate expressing a pre-fusion stabilized SARS-CoV-2 spike protein.
Odile Launay, Cécile Artaud, Marie Lachâtre, Mohand Ait-Ahmed, Jelle Klein, Liem Binh Luong Nguyen, Christine Durier, Bastiaan Jansen, Yvonne Tomberger, Nathalie Jolly, Anna Grossmann, Houda Tabbal, Jérémy Brunet, Marion Gransagne, Zaineb Choucha, Damien Batalie, Ana Delgado, Matthias Müllner, Roland Tschismarov, Pieter-Jan Berghmans, Annette Martin, Katrin Ramsauer, Nicolas Escriou, Christiane Gerke

2417 related Products with: Safety and immunogenicity of a measles-vectored SARS-CoV-2 vaccine candidate, V591 / TMV-083, in healthy adults: results of a randomized, placebo-controlled Phase I study.

200ul100ug Lyophilized10mg25mg 1 G200ul100ug Lyophilized 500 G200ul250ul

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#35045269   2022/01/19 To Up

The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.

SARS-CoV-2 spike (S) glycoprotein mediates viral entry and membrane fusion. Its cleavage at S1/S2 and S2' sites during the biosynthesis in virus-producer cells and viral entry are critical for viral infection and transmission. In contrast, the biological significance of the junction region between both cleavage sites on S protein synthesis and function is less understood. By analyzing the conservation and structure of S protein, we find that intra-chain contacts formed by the conserved tyrosine (Y) residue 756 (Y756) with three α helices contribute to the spike's conformational stability. When Y756 is mutated to an amino acid residue that can provide hydrogen bonds, S protein could be expressed as a cleaved form, but not . Also, the L753 mutation linked to the Y756 hydrogen bond prevents the S protein from being cleaved. Y756 and L753 mutations alter S protein subcellular localization. Importantly, Y756 and L753 mutations are demonstrated to reduce the infectivity of the SARS-CoV-2 pseudoviruses by interfering with the incorporation of S protein into pseudovirus particles and causing the pseudovirus to lose its sensitivity to neutralizing antibodies. Furthermore, both mutations affect the assembly and production of SARS-CoV-2 virus-like particles in cell culture. Altogether, our findings reveal for the first time a critical role for the conserved L753-LQ-Y756 motif between S1/S2 and S2' cleavage sites in S proteins synthesis and processing as well as virus assembly and infection. The continuous emergence of SARS-CoV-2 virus variants such as delta or lambda lineage caused the continuation of the epidemic and challenged the effectiveness of these existing vaccines. Logically, the spike (S) protein mutation has attracted much concern. However, the key amino acids in S protein for its structure and function are still not very clear. Here, we discovered for the first time that the conserved residues Y756 and L753 at the junction between S1/S2 and S2' sites are very important as the S2' cleavage site R815 for the synthesis and processing of S protein such as protease cleavage, and then the mutations severely interfered with the incorporation of S protein into pseudotyped virus particles and SARS-CoV-2 virus-like particles. Consequently, we delineate the novel potential target for the design of broad-spectrum antiviral drugs in the future, especially in the emergence of SARS-CoV-2 variants.
Shouwen Du, Wang Xu, Yuhang Wang, Letian Li, Pengfei Hao, Mingyao Tian, Maopeng Wang, Tiyuan Li, Shipin Wu, Quan Liu, Jieying Bai, Xiaoyun Qu, Ningyi Jin, Boping Zhou, Ming Liao, Chang Li

1647 related Products with: The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.

20 ug Product tipe: Antib100 100 100 100 100 10005001001001000100

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#35045226   2022/01/19 To Up

Immunogenicity and Reactogenicity of Vaccine Boosters after Ad26.COV2.S Priming.

The Ad26.COV2.S vaccine, which was approved as a single-shot immunization regimen, has been shown to be effective against severe coronavirus disease 2019. However, this vaccine induces lower severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S)-specific antibody levels than those induced by messenger RNA (mRNA)-based vaccines. The immunogenicity and reactogenicity of a homologous or heterologous booster in persons who have received an Ad26.COV2.S priming dose are unclear.
Roos S G Sablerolles, Wim J R Rietdijk, Abraham Goorhuis, Douwe F Postma, Leo G Visser, Daryl Geers, Katharina S Schmitz, Hannah M Garcia Garrido, Marion P G Koopmans, Virgil A S H Dalm, Neeltje A Kootstra, Anke L W Huckriede, Melvin Lafeber, Debbie van Baarle, Corine H GeurtsvanKessel, Rory D de Vries, P Hugo M van der Kuy,

1699 related Products with: Immunogenicity and Reactogenicity of Vaccine Boosters after Ad26.COV2.S Priming.

50 ug 100ug50 ug 100ug50 ug 100ug96T100ug50

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#35045183   // To Up

Clinical Profile and Short-Term Outcome of SARS-CoV-2-Infected Neonates from a Government Medical College in West Bengal, India.

Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) has led to a terrifying global pandemic. The presentations in neonates are varied with less case severity compared to adults.
Mukut Banerjee, Jonaki Pal, Tanushree Mondal, Taraknath Ghosh, Kaustav Nayek

1188 related Products with: Clinical Profile and Short-Term Outcome of SARS-CoV-2-Infected Neonates from a Government Medical College in West Bengal, India.

100 μg100 μg 0.1 mg

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#35044938   2021/12/31 To Up

COVID-19 in Malaysia: exposure assessment and prevention practices among healthcare workers at a teaching hospital.

During the second wave of the coronavirus disease 19 (COVID-19) pandemic, Malaysia reported several COVID-19 clusters related to healthcare workers. Thus, addressing and understanding the risk of exposure in healthcare workers is important to prevent future infection and reduce secondary COVID-19 transmission within the healthcare settings. In this study, we aim to assess exposure and prevention practices against COVID-19 among healthcare workers at the Hospital Canselor Tuanku Muhriz, a university teaching hospital based in Kuala Lumpur, Malaysia.
Nor Azila Muhammad Azami, Nor Azian Abdul Murad, Azmawati Mohammed Nawi, Sharifah Azura Salleh, Petrick Periyasamy, Najma Kori, Mohd Rohaizat Hasan, Norfazilah Ahmad, Anita Sulong, Hanita Othman, Tuti Ningseh Mohd Don, Nurul Syakima Ab Mutalib, Ezanee Azlina Mohamad Hanif, Siti Aishah Sulaiman, Nurul Syeefa' Zulkiflee, Abdul Rashid Abdul Kader, Abdul Halim Abdul Gafor, Hanafiah Haruna Rashid, Rahman Jamal

2719 related Products with: COVID-19 in Malaysia: exposure assessment and prevention practices among healthcare workers at a teaching hospital.

20 100 μg1 Set1 Set20 100 μg100 µl (2 mM)100 μg5 mg100 μg4 Membranes/Box5 mg

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#35044824   2022/01/19 To Up

Up-regulation of proBDNF/p75 signaling in antibody-secreting cells drives systemic lupus erythematosus.

Inappropriate expansion of antibody-secreting cells (ASCs) is typical of systemic lupus erythematosus (SLE), but the regulatory signaling of pathogenic ASCs is unclear. The present study shows that brain-derived neurotrophic factor precursor (proBDNF) and its high-affinity pan-75 neurotrophin receptor (p75) are highly expressed in CD19CD27CD38 ASCs in patients with SLE and in CD19CD44CD138 ASCs in lupus-like mice. The increased proBDNF ASCs were positively correlated with clinical symptoms and higher titers of autoantibodies in SLE. Administration of monoclonal antibodies against proBDNF or specific knockout of p75 in CD19 B cells exerted a therapeutic effect on lupus mice by limiting the proportion of ASCs, reducing the production of autoantibodies and attenuating kidney injury. Blocking the biological function of proBDNF or p75 also inhibits ASC differentiation and antibody production in vitro. Together, these findings suggest that proBDNF-p75 signaling plays a critical pathogenic role in SLE through promoting ASC dysfunction.
Wei-Yun Shen, Cong Luo, Plinio Reinaldo Hurtado, Xiao-Jing Liu, Ru-Yi Luo, Hui Li, Zhao-Lan Hu, Jun-Mei Xu, Elizabeth J Coulson, Ming Zhao, Xin-Fu Zhou, Ru-Ping Dai

2710 related Products with: Up-regulation of proBDNF/p75 signaling in antibody-secreting cells drives systemic lupus erythematosus.

96 wells1 Set1 Set1 Set1 Set8 Sample Kit50ug11 inhibitors100ug1 Set100ug Lyophilized1 Set

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#35044813   2022/01/19 To Up

From structure to sequence: Antibody discovery using cryoEM.

One of the rate-limiting steps in analyzing immune responses to vaccines or infections is the isolation and characterization of monoclonal antibodies. Here, we present a hybrid structural and bioinformatic approach to directly assign the heavy and light chains, identify complementarity-determining regions, and discover sequences from cryoEM density maps of serum-derived polyclonal antibodies bound to an antigen. When combined with next-generation sequencing of immune repertoires, we were able to specifically identify clonal family members, synthesize the monoclonal antibodies, and confirm that they interact with the antigen in a manner equivalent to the corresponding polyclonal antibodies. This structure-based approach for identification of monoclonal antibodies from polyclonal sera opens new avenues for analysis of immune responses and iterative vaccine design.
Aleksandar Antanasijevic, Charles A Bowman, Robert N Kirchdoerfer, Christopher A Cottrell, Gabriel Ozorowski, Amit A Upadhyay, Kimberly M Cirelli, Diane G Carnathan, Chiamaka A Enemuo, Leigh M Sewall, Bartek Nogal, Fangzhu Zhao, Bettina Groschel, William R Schief, Devin Sok, Guido Silvestri, Shane Crotty, Steven E Bosinger, Andrew B Ward

2364 related Products with: From structure to sequence: Antibody discovery using cryoEM.

3 modules1 module2 modules1 module200 ug1 module1 module1 module1 module1 module100 ul 50 UG

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#35044724   2022/01/19 To Up

Antiphospholipid antibodies increase endometrial stromal cell decidualization, senescence and inflammation via TLR4, ROS and p38 MAP kinase signaling.

Miscarriage affects one in seven pregnancies and antiphospholipid autoantibodies (aPL) are one of the biggest risk factors for recurrent pregnancy loss. While aPL target the endometrial stroma, little is known about their impact. Endometrial stromal cells (EnSCs) undergo decidualization each menstrual cycle, priming the uterus to receive implanting embryos. Thus, appropriate decidualization and EnSC function is key for establishment of a successful pregnancy.
Mancy Tong, Teimur Kayani, Deidre M Jones, Jane E Salmon, Shannon Whirledge, Lawrence W Chamley, Vikki M Abrahams

1328 related Products with: Antiphospholipid antibodies increase endometrial stromal cell decidualization, senescence and inflammation via TLR4, ROS and p38 MAP kinase signaling.

2 Pieces/Box0.1 mg1 ml1000 TESTS/0.65ml2 Pieces/Box100ug/vial100.00 ugOne Vial: 5 X 10^6 Cells 25 MG5mg100ug Lyophilized1000 assays

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#35044720   2022/01/19 To Up

High throughput glycosylation analysis of intact monoclonal antibodies by mass spectrometry coupled with capillary electrophoresis and liquid chromatography.

The analysis of monoclonal antibodies glycosylation is a crucial quality control attribute of biopharmaceutical drugs. High throughput screening approaches for antibody glycoform analysis are required in various stages of process optimization. Here, we present high throughput screening suitable mass spectrometry-based workflows for the analysis of intact antibody glycosylation out of cell supernatants. Capillary electrophoresis and liquid chromatography were coupled with quadrupole time-of-flight MS or Orbitrap MS. Both separation methods offer fast separation (10-15 min) and the capability to prevent the separated cell supernatant matrix to enter the MS by post-separation valving. Both MS instruments provide comparable results and both are sufficient to determine the glycosylation pattern of the five major glycoforms of the measured antibodies. However, the Orbitrap yields higher sensitivity of 25 μg/mL (CE-nanoCEasy-Orbitrap MS) and 5 μg/mL (LC-Orbitrap MS). Data processing was optimized for a faster processing and easier detection of low abundant glycoforms based on averaged charge-deconvoluted mass spectra. This approach combines a non-target glycoform analysis, while yielding the same glycosylation pattern as the traditional approach based on extracted ion traces. The presented methods enable the high throughput screening of the glycosylation pattern of antibodies down to low μg/mL-range out of cell supernatant without any sample preparation. This article is protected by copyright. All rights reserved.
Lukas Naumann, Patrick Schlossbauer, Florian Klingler, Friedemann Hesse, Kerstin Otte, Christian Neusüß

1320 related Products with: High throughput glycosylation analysis of intact monoclonal antibodies by mass spectrometry coupled with capillary electrophoresis and liquid chromatography.

100.00 ug100 ul200 ug1 mg100.00 ug2 Pieces/Box1 mg1 mg100.00 ug100.00 ug0.5 mg1 ml

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