Search results for: Antigen
#32251995 2020/03/30 To Up
A comparative study of peptide-based imaging agents [Ga]Ga-PSMA-11, [Ga]Ga-AMBA, [Ga]Ga-NODAGA-RGD and [Ga]Ga-DOTA-NT-20.3 in preclinical prostate tumour models.Peptide-based imaging agents targeting prostate-specific membrane antigen (PSMA) have revolutionized the evaluation of biochemical recurrence of prostate cancer (PCa) but lacks sensitivity at very low serum prostate specific antigen (PSA) levels. Once recurrence is suspected, other positron emission tomography (PET) radiotracers could be of interest to discriminate between local and distant relapse. We studied [F]fluorodeoxyglucose ([F]FDG) targeting glucose metabolism, [F]fluorocholine ([F]FCH) targeting membrane metabolism and peptide-based imaging agents [Ga]Ga-PSMA-11, [Ga]Ga-AMBA, [Ga]Ga-NODAGA-RGD and [Ga]Ga-DOTA-NT-20.3 targeting PSMA, gastrin releasing peptide receptor (GRPr), αvβ3 integrin and neurotensin type 1 receptor (NTSR1) respectively, in different PCa tumour models.
Jules Zhang-Yin, Claire Provost, Géraldine Cancel-Tassin, Timofei Rusu, Mallaurie Penent, Camelia Radulescu, Eva Comperat, Olivier Cussenot, Françoise Montravers, Raphaële Renard-Penna, Jean-Noël Talbot, Aurélie Prignon
2116 related Products with: A comparative study of peptide-based imaging agents [Ga]Ga-PSMA-11, [Ga]Ga-AMBA, [Ga]Ga-NODAGA-RGD and [Ga]Ga-DOTA-NT-20.3 in preclinical prostate tumour models.50 ug50 ug10 mg50 ug50 ug
#32251937 2020/03/27 To Up
Determination of the roles of GREM1 gene in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical follicles.Gremlin genes are known members of the DAN family of bone morphogenetic protein (BMP) antagonists, but their functions and regulatory mechanisms in ovarian follicular development of chicken remain unknown. The current study was designed to investigate the mRNA expression patterns of gremlin1 gene (GREM1) and its protein location in the follicles sampled, and to explore the biological effect of GREM1 on the prehierarchical follicular development. This work revealed that chicken GREM1 mRNA exhibits a constant expression level across all the prehierarchical follicles (PFs) from 1-4 mm to 7-8 mm in diameter, and the preovulatory follicles (from F6 to F1) by using RT-qPCR (P > 0.05). The GREM1 protein is predominantly expressed in the oocytes and granulosa cells (GCs) of the PFs by immunohistochemistry. Furthermore, our data demonstrated that siRNA-mediated knockdown of GREM1 in the GCs resulted in a significant reduction in cell proliferation (P < 0.001); conversely, overexpression of GREM1 in the GCs led to a remarkable increase in cell proliferation (P < 0.001). Interestingly, the expression levels of proliferating cell nuclear antigen (PCNA) and cyclin D2 (CCND2) mRNA and proteins were notably increased when GREM1 expression was upregulated in the GCs (P < 0.01), however, the expression levels of CYP11A1 and StAR were markedly downregulated (P < 0.01). The current results showed that GREM1 gene plays a stimulatory role in GC proliferation during growth and development of the prehierarchical follicles in vitro but an inhibitory role in GC differentiation and steroidogenesis of the hen ovary follicles.
Ning Qin, Thobela Louis Tyasi, Xue Sun, Xiaoxia Chen, Hongyan Zhu, Jinghua Zhao, Rifu Xu
2469 related Products with: Determination of the roles of GREM1 gene in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical follicles.25 ml. 5 G100 ml.96 samples1.00 flask100ug
#32251926 2020/03/31 To Up
Review of precision cancer medicine: Evolution of the treatment paradigm.In recent years, biotechnological breakthroughs have led to identification of complex and unique biologic features associated with carcinogenesis. Tumor and cell-free DNA profiling, immune markers, and proteomic and RNA analyses are used to identify these characteristics for optimization of anticancer therapy in individual patients. Consequently, clinical trials have evolved, shifting from tumor type-centered to gene-directed, histology-agnostic, with innovative adaptive design tailored to biomarker profiling with the goal to improve treatment outcomes. A plethora of precision medicine trials have been conducted. The majority of these trials demonstrated that matched therapy is associated with superior outcomes compared to non-matched therapy across tumor types and in specific cancers. To improve the implementation of precision medicine, this approach should be used early in the course of the disease, and patients should have complete tumor profiling and access to effective matched therapy. To overcome the complexity of tumor biology, clinical trials with combinations of gene-targeted therapy with immune-targeted approaches (e.g., checkpoint blockade, personalized vaccines and/or chimeric antigen receptor T-cells), hormonal therapy, chemotherapy and/or novel agents should be considered. These studies should target dynamic changes in tumor biologic abnormalities, eliminating minimal residual disease, and eradicating significant subclones that confer resistance to treatment. Mining and expansion of real-world data, facilitated by the use of advanced computer data processing capabilities, may contribute to validation of information to predict new applications for medicines. In this review, we summarize the clinical trials and discuss challenges and opportunities to accelerate the implementation of precision oncology.
Apostolia M Tsimberidou, Elena Fountzilas, Mina Nikanjam, Razelle Kurzrock
1943 related Products with: Review of precision cancer medicine: Evolution of the treatment paradigm.5 G
#32251655 2020/04/03 To Up
Increased cPRA is associated with increased waitlist time and mortality in LTx.Sensitized candidates with unacceptable antigens are a group that demands special attention in organ transplantation. Calculated panel reactive antigen (cPRA) is not used to modify allocation priorities in lung transplantation. The impact of cPRA on waiting list time and mortality is unknown METHODS: We performed a retrospective review of candidates for lung transplantation listed from May, 2005 to 2018. Data from OPTN/UNOS Standard Analysis and Research (STAR) dataset was paired with additional unacceptable antigen (UA-HLA) data, which was used to calculate the listing cPRA. Candidates were stratified based upon lack of UA-HLA or cPRA level for candidates with unacceptable antigens reported. Unadjusted competing risks and adjusted sub-distribution hazard models were fit.
Yaron D Barac, Mike S Mulvihill, Oliver Jawitz, Jacob Klapper, John Haney, Mani Daneshmand, Basil Nasir, Dongfeng Chen, Carmelo A Milano, Matthew G Hartwig
2627 related Products with: Increased cPRA is associated with increased waitlist time and mortality in LTx.1mg0.1ml100ug Lyophilized 0.1 mg 100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized
#32251650 2020/04/03 To Up
Age-related decline of lymphatic drainage from the eye: A noninvasive in vivo photoacoustic tomography study.We aim to determine whether lymphatic drainage from the eye changes with age. Using quantitative photoacoustic tomography, a group of young and older mice were studied in the live state. 10 CD-1 mice of 2-3 months (5M/5F) were studied in addition to 13 older mice of 12-13 months (6M/7F). In each of 23 mice, near-infrared tracer (a near-infrared dye, QC-1 conjugated with Bovine Serum Albumin) was injected into the right eye, and imaging of ipsilateral cervical lymph nodes was performed with laser pulses at 11 different wavelengths prior to and 20 min, 2, 4 and 6 h after injection. Mean pixel intensities (MPIs) of nodes were calculated at each imaging session. The areas under the curves (AUC) were calculated for both groups of mice and compared using the t-test. The slopes of MPI of each region of interest were compared using the linear mixed model before and after adjusting for sex, body weight and intraocular pressure of the right eye. The mean intraocular pressure of right eyes before injection was similar in older and younger groups (12.77 ± 2.01 mmHg and 12.90 ± 2.38 mmHg, respectively; p = 0.888). In each mouse, the photoacoustic signal was detected in the right cervical lymph nodes at the 2-h time point following tracer injection into the right eye. At 4- and 6-h imaging, a steady increase of tracer signal was observed. Areas under the curve in the right cervical nodes were decreased significantly in older mice compared to younger mice (p = 0.007). The slopes of MPI in the nodes were significantly decreased in old mice compared to young mice both before and after adjusting for sex, body weight and intraocular pressure of the right eye (p = 0.003). In conclusion, lymphatic drainage from the eye is significantly reduced in older eyes.This finding suggests that impaired lymphatic clearance of aqueous humor, proteins and antigens from the eye may contribute to age-related disease of the eye such as glaucoma and inflammatory eye disease.
Yeni H Yücel, Fang Cheng, Kirsten Cardinell, Xun Zhou, Hyacinth Irving, Neeru Gupta
1295 related Products with: Age-related decline of lymphatic drainage from the eye: A noninvasive in vivo photoacoustic tomography study.2 Pieces/Box48 samples2 Pieces/Box2 Pieces/Box2 Pieces/Box1
#32251641 2020/04/03 To Up
Safety and immunogenicity of a parenteral trivalent P2-VP8 subunit rotavirus vaccine: a multisite, randomised, double-blind, placebo-controlled trial.A monovalent, parenteral, subunit rotavirus vaccine was well tolerated and immunogenic in adults in the USA and in toddlers and infants in South Africa, but elicited poor responses against heterotypic rotavirus strains. We aimed to evaluate safety and immunogenicity of a trivalent vaccine formulation (P2-VP8-P,,).
Michelle J Groome, Lee Fairlie, Julie Morrison, Alan Fix, Anthonet Koen, Maysseb Masenya, Lisa Jose, Shabir A Madhi, Nicola Page, Monica McNeal, Len Dally, Iksung Cho, Maureen Power, Jorge Flores, Stanley Cryz
2539 related Products with: Safety and immunogenicity of a parenteral trivalent P2-VP8 subunit rotavirus vaccine: a multisite, randomised, double-blind, placebo-controlled trial.100ug96 wells (1 kit)1 ml100 ul1 mg5mg1 mg1 g 100ul 100ul100μg
#32251583 2020/04/06 To Up
Bioinspired Superwettable Micro-Spine Chips with Directional Droplet Transportation for Biosensing.Directional droplet transportation without extra energy input remains a challenge in microfluidic biochips for clinical detections. Herein, inspired by the water-collecting behaviors on the cactus spine, we fabricate nanomaterial-based superwettable micro-spine (SMS) chips. The bioinspired SMS chips are capable of spontaneous and directional droplet transportation by synergistically combining geometric asymmetry and surface superhydrophilicity. Based on theoretical models, the gradient of the Laplace pressure arising from the geometric asymmetry of SMS chip can dominate the directional transportation of droplet, and the superhydrophilicity of the nanomaterial-based micro-spine can also contribute to the droplet self-transportation. The multi-microchannel SMS chips provide a simple and energy efficient technology to realize accurate detection of serum prostate-specific antigen (PSA) from prostate cancer patients, showing great potential as a biosensing platform for clinical applications. We believe that our bioinspired superwettable two-dimensional conical surface will offer effective means for the design of smart microfluidic devices and have great potential applications in multicomponent biosensing and clinical detections.
Yanxia Chen, Kan Li, Shudong Zhang, Lei Qin, Shaohui Deng, Liyuan Ge, Li-Ping Xu, Lulin Ma, Shutao Wang, Xueji Zhang
1313 related Products with: Bioinspired Superwettable Micro-Spine Chips with Directional Droplet Transportation for Biosensing.5 g30 micro gram20000 (in case - Bulk) 100ul 100 G10 mg100 mg10 mg 1 G
#32251478 2020/04/06 To Up
Gluten tolerance prevents oral sensitization with enzymatic or acid hydrolyzed gluten: A study in Brown Norway rats.There are several reports describing allergy to hydrolyzed wheat products. After a large outbreak in Japan it was established that sensitization was caused by skin contact with acid hydrolyzed gluten in soap. It is still not clear if other forms of hydrolyzed gluten may sensitize, and if the skin is the only relevant route of sensitization in humans and to what extent oral tolerance to wheat play a role.
Charlotte Bernhard Madsen, Katrine Lindholm Bøgh
2842 related Products with: Gluten tolerance prevents oral sensitization with enzymatic or acid hydrolyzed gluten: A study in Brown Norway rats.100ul 100 G100ug
#32251470 2020/04/06 To Up
Cell-mediated and serology-based tests for Mycobacterium ulcerans disease: A systematic review and meta-analysis.Buruli ulcer (BU) is a subcutaneous necrotic infection of the skin caused by Mycobacterium ulcerans. It is the third most common human mycobacterial disease after tuberculosis (TB) and leprosy. The available methods for detection of the bacilli in lesions are microscopic detection, isolation and cultivation of the bacterium, histopathology, and polymerase chain reaction (PCR). These methods, although approved by the World Health Organization (WHO), have infrastructural and resource challenges in medical centres and cell-mediated immunity (CMI) and/or serology-based tests have been suggested as easier and more appropriate for accurate assessment of the disease, especially in remote or underdeveloped areas. This study systematically reviewed and conducted a meta-analysis for all research aimed at developing cell-mediated immunity (CMI) and/or serology-based tests for M. ulcerans disease. Information for this review was searched through PubMed and Web of Science databases and identified up to June 2019. References from relevant articles and reports from the WHO Annual Meeting of the Global Buruli Ulcer Initiative were also used. Twelve studies beginning in 1952, that attempted to develop CMI and/or serology-based tests for the disease were identified. These studies addressed issues of specificity and sensitivity in context of antigen composition as well as study heterogeneity and bias. The two main types of antigenic preparations considered were pathogen-derived and recombinant protein preparations. There was slight difference in test performance when M. ulcerans recombinant proteins [positivity: 67.5%; 32.5%] or pathogen-derived [positivity: 76.0%; 24.0%] preparations were used as test antigens among BU patients. However, pathogen-derived preparations were better at differentiating between patients and control groups [odds ratio (OR) of 27.92, 95%CI: 5.05-154.28]. This was followed by tests with the recombinant proteins [OR = 1.23, 95%CI: 0.27-5.62]. Overall, study heterogeneity index, I2 was 92.4% (p = 0.000). It is apparent from this review that standardisation is needed in any future CMI and/or serology-based tests used for M. ulcerans disease.
Michael S Avumegah, Nilakshi T Waidyatillake, Wojtek P Michalski, Daniel P O'Brien, Tiffanie M Nelson, Eugene Athan
1316 related Products with: Cell-mediated and serology-based tests for Mycobacterium ulcerans disease: A systematic review and meta-analysis.1000 TESTS/0.65ml1000 tests1000 tests1,000 tests1,000 tests1000 tests1000 tests200 25 mg25 mg100ug 5 G
#32251437 2020/04/06 To Up
Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.Lymph nodes (LNs) are highly organized secondary lymphoid organs that mediate adaptive immune responses to antigens delivered via afferent lymphatic vessels. Lymphatic endothelial cells (LECs) line intranodal lymphatic sinuses and organize lymph and antigen distribution. LECs also directly regulate T cells, mediating peripheral tolerance to self-antigens, and play a major role in many diseases, including cancer metastasis. However, little is known about the phenotypic and functional heterogeneity of LN LECs. Using single-cell RNA sequencing, we comprehensively defined the transcriptome of LECs in murine skin-draining LNs and identified new markers and functions of distinct LEC subpopulations. We found that LECs residing in the subcapsular sinus (SCS) have an unanticipated function in scavenging of modified low-density lipoprotein (LDL) and also identified a specific cortical LEC subtype implicated in rapid lymphocyte egress from LNs. Our data provide new, to our knowledge, insights into the diversity of LECs in murine LNs and a rich resource for future studies into the regulation of immune responses by LN LECs.
Noriki Fujimoto, Yuliang He, Marco D'Addio, Carlotta Tacconi, Michael Detmar, Lothar C Dieterich
2386 related Products with: Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask96 assays2 ml1.00 flask1.00 flask
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