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Search results for: Apelin

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#32740407   2020/07/27 To Up

The Elabela in hypertension, cardiovascular disease, renal disease, and preeclampsia: an update.

: Although considerable success has been shown for antihypertensive medications, the resistant hypertension and hypertension-related organ damages are still the important clinical issues and pose as high health and economic pressure. Therefore, novel therapeutic techniques and antihypertensive drugs are needed to advance more effective therapy of hypertension and hypertension-related disease to ameliorate mortality and healthcare costs worldwide. In this review, we highlight the latest progress in supporting the therapeutic potential of Elabela (ELA), a recently discovered early endogenous ligand for G-protein-coupled receptor apelin peptide jejunum, apelin receptor. Systemic administration of ELA exerts vasodilatory, antihypertensive, cardioprotective, and renoprotective effects, whereas central application of ELA increases blood pressure and causes cardiovascular remodeling primarily secondary to the hypertension. In addition, ELA drives extravillous trophoblast differentiation and prevents the pathogenesis of preeclampsia (a gestational hypertensive syndrome) by promoting placental angiogenesis. These findings strongly suggest peripheral ELA's therapeutic potential in preventing and treating hypertension and hypertension-related diseases including cardiovascular disease, kidney disease, and preeclampsia. Since therapeutic use of ELA is mainly limited by its short half-life and parenteral administration, it may be a clinical application candidate for the therapy of hypertension and its complications when fused with a large inert chemicals (e.g. polyethylene glycol, termed polyethylene glycol-ELA-21) or other proteins (e.g. the Fc fragment of IgG and albumin, termed Fc-ELA-21 or albumin-ELA-21), and new delivery methods are encouraged to develop to improve the efficacy of ELA fragments on apelin peptide jejunum or alternative unknown receptors.
Chuanming Xu

1392 related Products with: The Elabela in hypertension, cardiovascular disease, renal disease, and preeclampsia: an update.

1-99 mg/ml/ea price x 2 50 UG100 1-99 mg/ml/ea price x 21 mg1-99 mg/ml/ea price x 21 mg

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#32729912   // To Up

The Role of Apelin/APJ in a Mouse Model of Oxygen-induced Retinopathy.

The aim of this study was to investigate apelin and its potential neovascularization role in retinopathy of prematurity (ROP) along with the inhibitory effects of its antagonist.
Jing Feng, Li Chen, Yanrong Jiang, Yong Tao

2689 related Products with: The Role of Apelin/APJ in a Mouse Model of Oxygen-induced Retinopathy.

50 ul100 ul100.00 ug100 μg100ug100 μg0.1ml100ug Lyophilized100 μg100.00 ug100.00 ug100.00 ug

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#32727063   2020/07/27 To Up

Transcriptome Profiling Associated with Carcass Quality of Loin Muscles in Crossbred Pigs.

Carcass quality traits, such as lean depth and loin depth, are of extreme economic importance for the swine industry. This study aimed to identify the gene expression pattern related to carcass quality in crossbred pigs ((Landrace × Yorkshire) × Duroc). In total, 20 crossbred pigs were used in this study and they were divided into two groups (class I grade, n = 10; class II grade, n = 10) based on the carcass grades. Total RNA samples extracted from the loin muscles of both groups were submitted for RNA-seq. The quality assessment of the sequencing reads resulted in 25,458 unigenes and found 12,795 candidate coding unigenes with homology to other species after annotation. Differentially expressed gene (DEG) analysis of the two groups revealed 282 up-regulated and 189 down-regulated genes ( ≤ 0.01), linked to tissue development, striated muscle tissue development, tissue morphogenesis, and lipid metabolic process gene ontology (GO) terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis highlighted genes related to the calcium signaling pathway, melanogenesis, the sphingolipid signaling pathway, the apelin signaling pathway, and the mTOR signaling pathway. We constructed an expressed gene profile, which may serve as a resource for genomic studies focused on uncovering the molecular mechanisms underlying carcass quality in crossbred pigs.
Sang-Mo Kim, Kesavan Markkandan, Jong-Young Lee, Gye-Woong Kim, Jae Young Yoo

2032 related Products with: Transcriptome Profiling Associated with Carcass Quality of Loin Muscles in Crossbred Pigs.

121 Set1 Set500 500 MG100.00 ug16 Arrays/Slide100 μg

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#32716043   2020/07/27 To Up

Serum apelin and resistin levels in patients with impaired fasting glucose, impaired glucose tolerance, Type 2 diabetes and metabolic syndrome.

In recent studies, it has been stated that adipose tissue is not only a source of energy, but also an active endocrine organ and that apelin and resistin excreted by this tissue are related to Type 2 diabetes and MeS. This study aims to compare and contrast serum apelin, resistin and insulin resistance parameters in patients with IFG and IGT, which are pre-diabetes phases, Type 2 DM and MeS. The study was comprised of patients with 18 type 2 DM (9 females, 9 males), 18 IFG (9 females, 9 males), 18 IGT (9 females, 9 males), 18 MeS (9 females, 9 males) and 16 healthy individuals (8 females, 8 males), and serum adiponectin, apelin, resistin levels, fasting and postprandial blood glucose, insulin resistance markers and lipid parameters were measured. In the study serum apelin levels were also determined to be significantly lower in IGT, MeS and Type 2 DM groups compared with control group (p<0.05; p<0.05 and p<0.001, respectively). As for serum resistin levels, they were determined to be significantly higher in IGT and Type 2 DM groups compared with control group (p<0.01 and p<0.001, respectively). However, no significant differences were determined in IFG group rather than control group, in terms of serum apelin and resistin levels. In conclusion, it may considered that apelin and resistin could influence glucose metabolism and insulin resistance; become a kind of useful indicator in preventing complications with early diagnosis in pre-diabetes phase of individuals with IGT, rather than IFG, and apelin could have a potential role in treatment.
Erhan Onalan, Burkay Yakar, Abdullah Onder Barım, Mehmet Ferit Gursu

2584 related Products with: Serum apelin and resistin levels in patients with impaired fasting glucose, impaired glucose tolerance, Type 2 diabetes and metabolic syndrome.

200ul200ul96T200ul2x96 well plate250ul96T250ul25ml96T250ul2 Pieces/Box

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#32713880   // To Up

Serum Elabela Levels Are Elevated in Patients with Hyperthyroidism.

The apelinergic system plays an important role in the modulation of the cardiovascular system via the apelin peptide and the apelin receptor (APJ receptor). Apelin and elabela, also known toddler, are peptide ligands for the apelin receptor. These two peptides show similar biological actions, such as vasodilatation, increased myocardial contractility, angiogenesis, and energy metabolism. However, the serum levels of elabela in patients with hyperthyroidism are not well known. The aim of this study was to investigate the changes in serum elabela levels in patients with hyperthyroidism and its association with hypertension. This cross-sectional study included 74 patients with newly diagnosed hyperthyroidism due to Graves' disease and 20 healthy individuals. Serum elabela levels were measured by enzyme-linked immunosorbent assay. The patients were divided into two groups: hyperthyroid patients without hypertension (n = 51) and those with hypertension (n = 23). Basal heart rate, serum glucose and high-sensitive C reactive protein were significantly higher in hyperthyroid patients with and those without hypertension than in healthy controls (p < 0.05 for each). Serum elabela levels were significantly elevated in hyperthyroid patients compared with healthy controls, with higher serum elabela levels found in hyperthyroid patients with hypertension than those without hypertension. Linear regression analysis showed that serum elabela levels were correlated with systolic blood pressure (p < 0.001). In conclusion, serum elabela levels were significantly increased in patients with hyperthyroidism, especially in hyperthyroid patients with hypertension. Elevation in serum elabela levels may contribute to alleviation of cardiovascular complications of hyperthyroidism and hypertension.
Mahmut Buyuksimsek, Erdinc Gulumsek, Muhammed Zubeyir Aslan, Huseyin Ali Ozturk, Ahmed Muhammad Bashir, Yahya Kemal Icen, Nurettin Ay, Fettah Acibucu, Mevlut Koc, Hilmi Erdem Sumbul, Tayyibe Saler

2079 related Products with: Serum Elabela Levels Are Elevated in Patients with Hyperthyroidism.

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#32713238   // To Up

Serum elabela and apelin levels during different stages of chronic kidney disease.

The association of serum elabela (ELA) and apelin with the progression of chronic kidney disease (CKD) is unknown. We determined if serum ELA and apelin levels were associated with CKD stage.
Xuehong Lu, Shengmao Liu, Rumei Luan, Wenpeng Cui, Yu Chen, Yixian Zhang, Yue Lu, Hong Zhang, Lin Shi, Lining Miao, Feng Xu

2207 related Products with: Serum elabela and apelin levels during different stages of chronic kidney disease.

50 UG96TEach100 ml200ug50ug 500ml1000ml1 mg

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#32708358   2020/07/18 To Up

Ubiquitin Pathway Is Associated with Worsening Left Ventricle Function after Mitral Valve Repair: A Global Gene Expression Study.

The molecular mechanism for worsening left ventricular (LV) function after mitral valve (MV) repair for chronic mitral regurgitation remains unknown. We wished to assess the LV transcriptome and identify determinants associated with worsening LV function post-MV repair. A total of 13 patients who underwent MV repair for chronic primary mitral regurgitation were divided into two groups, preserved LV function (N = 8) and worsening LV function (N = 5), for the study. Specimens of LV from the patients taken during surgery were used for the gene microarray study. Cardiomyocyte cell line HL-1 cells were transfected with gene-containing plasmids and further evaluated for mRNA and protein expression, apoptosis, and contractile protein degradation. Of 67,258 expressed sequence tags, microarrays identified 718 genes to be differentially expressed between preserved-LVF and worsening-LVF, including genes related to the protein ubiquitination pathway, bone morphogenetic protein (BMP) receptors, and regulation of eIF4 and p70S6K signaling. In addition, worsening-LVF was associated with altered expressions of genes pathologically relevant to heart failure, such asdownregulated apelin receptors and upregulated peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A). HL-1 cardiomyocyte cells transfected with ubiquitination-related genes demonstrated activation of the protein ubiquitination pathwaywith an increase in the ubiquitin activating enzyme E1 (UAE-E1). It also led to increased apoptosis, downregulated and ubiquitinated X-linked inhibitor of apoptosis protein (XIAP), and reduced cell viability. Overexpression of ubiquitination-related genes also resulted in degradation and increased ubiquitination of α-smooth muscle actin (SMA). In conclusion, worsening-LVF presented differential gene expression profiles from preserved-LVF after MV repair. Upregulation of protein ubiquitination-related genes associated with worsening-LVF after MV repair may exert adverse effects on LV through increased apoptosis and contractile protein degradation.
Feng-Chun Tsai, Gwo-Jyh Chang, Ying-Ju Lai, Shang-Hung Chang, Wei-Jan Chen, Yung-Hsin Yeh

2280 related Products with: Ubiquitin Pathway Is Associated with Worsening Left Ventricle Function after Mitral Valve Repair: A Global Gene Expression Study.

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#32698411   2020/07/20 To Up

Apelin Improves Prognostic Value of HFSS (Heart Failure Survival Score) and MAGGIC (Meta-Analysis Global Group in Chronic Heart Failure) Scales in Ambulatory Patients with End-Stage Heart Failure.

This prospective study aimed to determine the effect of adding apelin to the MAGGIC (Meta-Analysis Global Group In Chronic Heart Failure) and HFSS (Heart Failure Survival Score) scales for predicting one-year mortality in 240 ambulatory patients accepted for heart transplantation (HT) between 2015-2017. The study also investigated whether the combination of N-terminal pro-brain natriuretic peptide (NT-proBNP) with MAGGIC or HFSS improves the ability of these scales to effectively separate one-year survivors from non-survivors on the HT waiting list. The median age of the patients was 58.0 (51.50.0-64.0) years and 212 (88.3%) of them were male. Within a one year follow-up, 75 (31.2%) patients died. The area under the curves (AUC) for baseline parameters was as follows-0.7350 for HFSS, 0.7230 for MAGGIC, 0.7992 for apelin and 0.7028 for NT-proBNP. The HFSS-apelin score generated excellent power to predict the one-year survival, with the AUC of 0.8633 and a high sensitivity and specificity (80% and 78%, respectively). The predictive accuracy of MAGGIC-apelin score was also excellent (AUC: 0.8523, sensitivity of 75%, specificity of 79%). The addition of NT-proBNP to the HFSS model slightly improved the predictive power of this scale (AUC: 0.7665, sensitivity 83%, specificity 60%), while it did not affect the prognostic strength of MAGGIC (AUC: 0.738, sensitivity 71%, specificity 69%). In conclusion, the addition of apelin to the HFSS and MAGGIC models significantly improved their ability to predict the one-year survival in patients with advanced HF. The MAGGIC-apelin and HFSS-apelin scores provide simple and powerful methods for risk stratification in end-stage HF patients. NT-proBNP slightly improved the prognostic power of HFSS, while it did not affect the predictive power of MAGGIC.
Wioletta Szczurek, Mariusz Gąsior, Michał Skrzypek, Bożena Szyguła-Jurkiewicz

2323 related Products with: Apelin Improves Prognostic Value of HFSS (Heart Failure Survival Score) and MAGGIC (Meta-Analysis Global Group in Chronic Heart Failure) Scales in Ambulatory Patients with End-Stage Heart Failure.

100 UG1 mL

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#32692577   2020/06/17 To Up

Susceptibility to high-altitude pulmonary edema is associated with circulating miRNA levels under hypobaric hypoxia conditions.

Hypobaric hypoxia poses stress to sojourners traveling to high-altitude (HA). A cascade of physiological changes occurs to cope with or adapt to hypobaric hypoxia. However, an insufficient physiological response to the hypoxic condition due to imbalanced vascular homeostasis pathways results in high-altitude pulmonary edema (HAPE). The present study aims to identify the implication of miRNAs associating with HAPE and adaptation. We analyzed the expression of 1113 miRNAs in HAPE patients (HAPE-p), HAPE-free controls (HAPE-f), and highland natives (HLs). Based on miRNA profiling and in silico analyses, miR-124-3p emerged relevantly. We observed a significant over-expression of miR-124-3p in HAPE-p. In silico analyses revealed a direct interaction of miR-124-3p with vascular homeostasis and hypoxia associated genes, NOS3 (Endothelial Nitric Oxide Synthase), Apelin, and ETS1 (V-Ets Avian Erythroblastosis Virus E2 Oncogene Homolog 1). Moreover, the transcript and bio-level expression of these genes were significantly decreased in HAPE-p when compared with HAPE-f or HLs. Our in vitro analysis in HUVEC demonstrated a significant knockdown of these genes both at transcript and protein levels following miR-124-3p over-expression. Conclusively, our results showed that miR-124-3p might play a plausible role in HAPE pathophysiology by inhibiting the expression of NOS3, Apelin, and ETS1.
Perwez Alam, Garima Agarwal, Rahul Kumar, Aastha Mishra, Neeru Saini, Ghulam Mohammad, M A Qadar Pasha

1491 related Products with: Susceptibility to high-altitude pulmonary edema is associated with circulating miRNA levels under hypobaric hypoxia conditions.

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#32687618   2020/07/20 To Up

ELABELA improvesendothelial cell function via the ELA-APJ axis by activating the PI3K/Akt-signaling pathway in HUVECs and EA.hy926 cells.

Destruction of endothelial cells (ECs) function isinvolved in the structural and functional pathophysiological processes of preeclampsia (PE). Vascular endothelial injury may pre-exist for several years in women that develop PE and may pose increased risks for hypertension, coronary artery disease, and type-2 diabetes mellitus. Previous findings showed that Elabela , the endogenous ligand of the apelin (APJ) receptor expressed mainly on ECs, may play a protective role in early pregnancy and prevent PE. However, the exact functional role and molecular mechanisms of ELA are unclear. Here, we aimed to classify whether and how ELA improves EC function viathe ELA-APJ axis. Two human umbilical vein endothelial cell (HUVEC) lines,namely HUVECs and EA.hy926, were treated with ELA, and then their cellular activities were studied by performing CCK-8 tests, scratch-wound analysis, and tube-formation assays. Doses of ELA exceeding 0.01 μM markedly improved the cell viability, migration, and tube formation ability of HUVECs and EA.hy926 cells. Western blot analysis indicated that the above effects caused by ELA were related to up-regulation of the APJ receptor and activation of PI3K/Akt signaling. Further verification tests were performed using the PI3K inhibitor wortmannin, and the results illustrated that inhibiting PI3K/Akt signaling blocked the positive effects of ELA on EC function and APJ receptor expression. Taken together, our findings indicate that ELA may alter EC function via the ELA-APJ axis and PI3K/Akt signaling and thatELA shows promise for usein endothelial dysfunction therapy for preventing and treating PE.
Xiujuan Wang, Guoqing Liang, Qing Guo, Wei Cai, Xin Zhang, Jianmei Ni, Yanyan Tao, Xiulong Niu, Shaobo Chen

1558 related Products with: ELABELA improvesendothelial cell function via the ELA-APJ axis by activating the PI3K/Akt-signaling pathway in HUVECs and EA.hy926 cells.

2 Pieces/Box1.5 x 10^6 cells1.5x10(6) cells10 ug100ìl x 10 vials100 ug/vial100 µg10 rxns1x10e7 cells1.00 flask2 Pieces/Box

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