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Search results for: (S)-3-Benzyloxycarbonyl-5-oxo-4-oxazolidinepropanoic Acid C14H15NO6 CAS: 23632-67-9

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#33946921   2021/04/29 To Up

Discovery of Siderophore and Metallophore Production in the Aerobic Anoxygenic Phototrophs.

Aerobic anoxygenic phototrophs have been isolated from a rich variety of environments including marine ecosystems, freshwater and meromictic lakes, hypersaline springs, and biological soil crusts, all in the hopes of understanding their ecological niche. Over 100 isolates were chosen for this study, representing 44 species from 27 genera. Interactions with Fe and other metal(loid) cations such as Mg, V, Mn, Co, Ni, Cu, Zn, Se and Te were tested using a chromeazurol S assay to detect siderophore or metallophore production, respectively. Representatives from 20 species in 14 genera of α-, or 30% of strains, produced highly diffusible siderophores that could bind one or more metal(loid)s, with activity strength as follows: Fe > Zn > V > Te > Cu > Mn > Mg > Se > Ni > Co. In addition, γ-proteobacterial , strain EG19 excreted a brown compound into growth medium, which was purified and confirmed to act as a siderophore. It had an approximate size of ~341 Da and drew similarities to the siderophore rhodotorulic acid, a member of the hydroxamate group, previously found only among yeasts. This study is the first to discover siderophore production to be widespread among the aerobic anoxygenic phototrophs, which may be another key method of metal(loid) chelation and potential detoxification within their environments.
Steven B Kuzyk, Elizabeth Hughes, Vladimir Yurkov

2993 related Products with: Discovery of Siderophore and Metallophore Production in the Aerobic Anoxygenic Phototrophs.

18 inhibitors3 inhibitors5 inhibitorsInhibitors6 inhibitors5 Inhibitors

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#33946173   2021/04/30 To Up

MES7 Modulates Seed Germination via Regulating Salicylic Acid Content in Arabidopsis.

Seed germination is an important phase transitional period of angiosperm plants during which seeds are highly sensitive to different environmental conditions. Although seed germination is under the regulation of salicylic acid (SA) and other hormones, the molecular mechanism underlying these regulations remains mysterious. In this study, we determined the expression of SA methyl esterase () family genes during seed germination. We found that expression decreases significantly in imbibed seeds, and the dysfunction of decreases SA content. Furthermore, reduces and promotes seed germination under normal and salt stress conditions, respectively. The application of SA restores the seed germination deficiencies of mutants under different conditions. Taking together, our observations uncover a MeSA hydrolytic enzyme, MES7, regulates seed germination via altering SA titer under normal and abiotic stress conditions.
Wenrui Gao, Yan Liu, Juan Huang, Yaqiu Chen, Chen Chen, Lu Lu, Hongwei Zhao, Shuzhen Men, Xiaoming Zhang

2078 related Products with: MES7 Modulates Seed Germination via Regulating Salicylic Acid Content in Arabidopsis.

1 G10 mg 100 G100ug Lyophilized1 g 1 G100ug 1KG100ug Lyophilized 100 G10 mg

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#33944994   2021/05/04 To Up

Complete genome sequence analysis of a novel coguvirus isolated from Paris polyphylla var. yunnanensis.

A novel negative-stranded (ns) RNA virus tentatively named "Yunnan paris negative-stranded virus" (YPNSV), was isolated from Paris polyphylla var. yunnanensis plants exhibiting leaf chlorosis and mosaic symptoms in Yunnan. Its complete genome sequence was determined using Illumina and Sanger sequencing. YPNSV has a bipartite genome that consists of a negative-stranded (ns) RNA1 encoding the viral RNA-dependent RNA polymerase (RdRp, p251), an ambisense RNA2 coding for the putative movement protein (MP, p46) and nucleocapsid protein (NP, p39), with the two open reading frames separated by a long intergenic region that is rich in A and U. Sequence comparisons showed that the RdRp, MP, and NP of YPNSV are most similar to those of watermelon crinkle leaf-associated virus 2 (WCLaV-2), with 69.1%, 50.4%, and 60.9% amino acid sequence identity, respectively. Phylogenetic analysis based on deduced amino acid sequences of RdRp and NP showed that YPNSV clustered in a clade with coguviruses and that its closest known relative is WCLaV-2. Based on the above results, YPNSV should be regarded as a new member of genus Coguvirus, family Phenuiviridae.
Zeli Chen, Rex Frimpong Anane, Zhe Wang, Like Gao, Lu Chen, Shangyun Li, Guosong Wen, Mingfu Zhao

1378 related Products with: Complete genome sequence analysis of a novel coguvirus isolated from Paris polyphylla var. yunnanensis.

1 module 15 ml 1 module1 module1 module1 module1 module1 module 1 kit(s) 1 module1 module

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#33941935   2021/05/03 To Up

Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes.

Competitive coevolution between microbes and viruses has led to the diversification of CRISPR-Cas defense systems against infectious agents. By analyzing metagenomic terabase datasets, we identified two compact families (775 to 803 amino acids (aa)) of CRISPR-Cas ribonucleases from hypersaline samples, named Cas13X and Cas13Y. We engineered Cas13X.1 (775 aa) for RNA interference experiments in mammalian cell lines. We found Cas13X.1 could tolerate single-nucleotide mismatches in RNA recognition, facilitating prophylactic RNA virus inhibition. Moreover, a minimal RNA base editor, composed of engineered deaminase (385 aa) and truncated Cas13X.1 (445 aa), exhibited robust editing efficiency and high specificity to induce RNA base conversions. Our results suggest that there exist untapped bacterial defense systems in natural microbes that can function efficiently in mammalian cells, and thus potentially are useful for RNA-editing-based research.
Chunlong Xu, Yingsi Zhou, Qingquan Xiao, Bingbing He, Guannan Geng, Zikang Wang, Birong Cao, Xue Dong, Weiya Bai, Yifan Wang, Xiang Wang, Dongming Zhou, Tanglong Yuan, Xiaona Huo, Jinsheng Lai, Hui Yang

1075 related Products with: Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes.

1 Product tipe: Instrumen25ul200ul11000 units10 nmol5 X 1000 U10 nmol50 mg

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#33940442   2021/04/30 To Up

Perfluoroalkyl substances in drinking water sources along the Yangtze River in Jiangsu Province, China: Human health and ecological risk assessment.

Perfluoroalkyl substances (PFASs) in source water is of growing concern for its adverse effects on human health and wildlife as well. The Yangtze River is the vital drinking water source in Jiangsu Province of China, but little attention has been paid on PFASs. The occurrence, spatial distribution and temporal trend of PFASs in 21 water sources along the Jiangsu section of the Yangtze River was investigated with sampling from 2018 to 2020. Moreover, health risk of PFASs was assessed by estimated intake dose and derived tolerable intake dose, while ecological risk was assessed by selected effect concentration and environmental exposure. PFASs concentrations in source water ranged from 12.0 to 128 ng/L, with perfluorooctanoic acid (PFOA) as the dominated congener. Fluorine chemical industry lead to a great increase of perfluorohexanoic acid (PFHxA) in its nearest water source. The estimated daily intake of PFASs through drinking was 0.54 and 0.82 ng/kg bw/day for adults and children. The major health risk was from perfluorooctane sulfonate (PFOS) and PFOA for their toxicity on liver, reproduction, development and immunity, with the maximum hazard quotient of 0.029 and 0.043 for adults and children in the worst scenario. The ecological risks from PFASs on nine species groups ranged from 2.7 × 10 to 5.2. PFOA and Perfluorobutane sulfonate (PFBS) were causing significant risk on wildlife, particularly on worms, mussels, and fish, which may further influence the structure and processes in the foodweb. Overall, PFASs, especially PFOS, PFOA and PFBS, induced considerable risk on human health and aquatic species in some hotspot area. It would be necessary to include them into monitoring in China and develop standards for different protection purposes.
Yueqing Zhang, Yunqiao Zhou, Aiguo Zhang, Juying Li, Jia Yu, Yezhi Dou, Jian He, Deyang Kong

2381 related Products with: Perfluoroalkyl substances in drinking water sources along the Yangtze River in Jiangsu Province, China: Human health and ecological risk assessment.

1 mg100 μg5 100 μg100 μg100 μg100 μg10 100 μg100 μg100 μg100μg

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#33940211   2021/04/30 To Up

A bacterial effector protein uncovers a plant metabolic pathway involved in tolerance to bacterial wilt disease.

Bacterial wilt caused by the soil-borne plant pathogen Ralstonia solanacearum is a devastating disease worldwide. Upon plant colonization, R. solanacearum replicates massively, causing plant wilting and death; collapsed infected tissues then serve as a source of inoculum. In this work, we show that the plant metabolic pathway mediated by pyruvate decarboxylases (PDCs) contributes to plant tolerance to bacterial wilt disease. Arabidopsis and tomato respond to R. solanacearum infection by increasing PDC activity, and plants with deficient PDC activity are more susceptible to bacterial wilt. Treatment with either pyruvic acid or acetic acid (substrate and product of the PDC pathway, respectively) enhances tolerance to disease. An effector protein secreted by R. solanacearum, RipAK, interacts with PDCs and inhibits their oligomerisation and enzymatic activity. This work reveals a metabolic pathway involved in resistance to biotic and abiotic stresses, and a bacterial virulence strategy to promote disease and the completion of the pathogenic life cycle.
Yaru Wang, Achen Zhao, Rafael J L Morcillo, Gang Yu, Hao Xue, Jose S Rufian, Yuying Sang, Alberto P Macho

2913 related Products with: A bacterial effector protein uncovers a plant metabolic pathway involved in tolerance to bacterial wilt disease.

100 1 mg100 50 UG100ug Lyophilized100ug Lyophilized1 Set1 Set1 Set

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#33939832   2021/05/03 To Up

HDAC3 controls male fertility through enzyme-independent transcriptional regulation at the meiotic exit of spermatogenesis.

The transition from meiotic spermatocytes to postmeiotic haploid germ cells constitutes an essential step in spermatogenesis. The epigenomic regulatory mechanisms underlying this transition remain unclear. Here, we find a prominent transcriptomic switch from the late spermatocytes to the early round spermatids during the meiotic-to-postmeiotic transition, which is associated with robust histone acetylation changes across the genome. Among histone deacetylases (HDACs) and acetyltransferases, we find that HDAC3 is selectively expressed in the late meiotic and early haploid stages. Three independent mouse lines with the testis-specific knockout of HDAC3 show infertility and defects in meiotic exit with an arrest at the late stage of meiosis or early stage of round spermatids. Stage-specific RNA-seq and histone acetylation ChIP-seq analyses reveal that HDAC3 represses meiotic/spermatogonial genes and activates postmeiotic haploid gene programs during meiotic exit, with associated histone acetylation alterations. Unexpectedly, abolishing HDAC3 catalytic activity by missense mutations in the nuclear receptor corepressor (NCOR or SMRT) does not cause infertility, despite causing histone hyperacetylation as HDAC3 knockout, demonstrating that HDAC3 enzyme activity is not required for spermatogenesis. Motif analysis of the HDAC3 cistrome in the testes identified SOX30, which has a similar spatiotemporal expression pattern as HDAC3 during spermatogenesis. Depletion of SOX30 in the testes abolishes the genomic recruitment of the HDAC3 to the binding sites. Collectively, these results establish the SOX30/HDAC3 signaling as a key regulator of the transcriptional program in a deacetylase-independent manner during the meiotic-to-postmeiotic transition in spermatogenesis.
Huiqi Yin, Zhenlong Kang, Yingwen Zhang, Yingyun Gong, Mengrou Liu, Yanfeng Xue, Wenxiu He, Yanfeng Wang, Shuya Zhang, Qiushi Xu, Kaiqiang Fu, Bangjin Zheng, Jie Xie, Jinwen Zhang, Yuanyuan Wang, Mingyan Lin, Yihan Zhang, Hua Feng, Changpeng Xin, Yichun Guan, Chaoyang Huang, Xuejiang Guo, P Jeremy Wang, Joseph A Baur, Ke Zheng, Zheng Sun, Lan Ye

2683 related Products with: HDAC3 controls male fertility through enzyme-independent transcriptional regulation at the meiotic exit of spermatogenesis.

100 U500 Units100 ul5 mg200 ug5 mg100ug100ug 100ul10100μg

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#33939141   2021/04/30 To Up

Carbonic Anhydrases II, IX, and XII in Reflux Esophagitis.

The pathogenesis of gastroesophageal reflux disease (GERD) has not been resolved in detail. Esophageal epithelial cells provide resistance to acidic reflux via several mechanisms, many of which involve buffering acid with bicarbonate and transporting protons. Carbonic anhydrases (CAs) are enzymes that control the acid-base balance by catalyzing the reversible hydration of carbon dioxide to produce bicarbonate and hydrogen ions.
Minna Nortunen, Nina Väkiparta, Seppo Parkkila, Juha Saarnio, Heikki Huhta, Tuomo J Karttunen

2146 related Products with: Carbonic Anhydrases II, IX, and XII in Reflux Esophagitis.

100 μg100 μg100 μg50ul 1 G20mg500 MG11 inhibitors16 Arrays/Slide200

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