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#32480285   2020/05/19 To Up

Impact of diet moisture on the development of the forensically important blow fly Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae).

The secondary screwworm, Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), is a carrion-breeding species of veterinary, medical, and forensic importance. It is very abundant in the Nearctic and Neotropical regions and is one of the most common colonizers of vertebrate remains in the southern United States. Therefore, it is of great evidential value in estimating the minimum time of colonization (TOC) of remains related to forensic investigations. So far, studies have investigated the effects of several biotic and abiotic factors on C. macellaria. However, no data on the specific impact of food source moisture on the larval development of this species are known to have been published. In this study, the effects of diet moisture on larval development time, larval length, and weight over time, as well as adult emergence and weight were investigated. C. macellaria was reared on diets prepared from freeze-dried bovine liver with varying moisture content (0.0, 33.0, 50.0, or 70.8%) at 25.6 °C, 77% RH, and 14:10 L:D. Frozen-thawed liver was used as a control. Water content was found to significantly impact immature development time and corresponding life-history traits, both within and among developmental stages of C. macellaria. This result indicates moisture content of the carrion source should be considered when estimating time of colonization and identification of immature stage of flies in forensic investigations. Furthermore, as diet moisture content significantly affected the dry mass of emerging adults, the mass of adults collected at a crime scene could provide useful information as it could be an indicator of the condition the remains were in during time of colonization, including the moisture content of the tissue.
Alexandra Bauer, Amely M Bauer, Jeffery K Tomberlin

2301 related Products with: Impact of diet moisture on the development of the forensically important blow fly Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae).

0.1 mg1 ml1100 IU1500 Units121 ml

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#32480277   2020/05/23 To Up

Interaction mechanism of flavonoids on bovine serum albumin: Insights from molecular property-binding affinity relationship.

The molecular structure properties-binding affinity relationship of a series of flavonoids and bovine serum albumin (BSA) was investigated in vitro from comparing the binding constants determined through the fluorescence method. As a result, the binding process was greatly influenced by different structural elements or substituents of flavonoids under analysis. The hydroxylation at the positions C3, C6, C4', C5' (for type I) and C5, C3' (for type II) were in favor of forming hydrogen bonds with the amino acids of BSA, which was of great importance in the binding and interaction between flavonoids and the protein. The decreased affinity could be realized by the methoxylation (C8, C3' and C4') and glycosylation (C3 and C7) of flavonoid type I. However, the adverse trend on binding affinity was observed when the methoxylation and glycosylation appeared at the sites C4' and C7, C4' of structure type II, respectively. Meanwhile, glycosylation at C7 mainly induced the decline in the affinity of flavonoids (type III), and the hydrogenation of the C2C3 double bond for type I was beneficial to increase the affinity on BSA. Moreover, part of flavonoids could mediate the conformational alteration of secondary structures of the protein during the interaction process, which was inferred by means of the synchronous fluorescence spectra. The determinations of ANS fluorescence probe suggested that hydrophobic interaction played an important role in the binding of a majority of flavonoids to BSA. Further evidences from the site-specific experiments revealed that the location of flavonoids 19, 29 and 34 binding on BSA mainly belonged to site I, while compound 3 bound to both sites I and II. Additionally, molecular modelling studies further confirmed the indispensable character of hydrophobic interaction and hydrogen bonds, and illustrated the preferred complex binding behaviors.
Hongjin Tang, Lin Huang, Dongsheng Zhao, Chunyong Sun, Ping Song

1521 related Products with: Interaction mechanism of flavonoids on bovine serum albumin: Insights from molecular property-binding affinity relationship.

100ug1 ml100ug100 μg100ul50ul100ug1 ml500 g Product tipe: Kit100ug200ul100ul

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#32480270   2020/05/22 To Up

Decisive role of pH in synthesis of high purity fluorescent BSA-Au nanoclusters.

Various types of bovine serum albumin (BSA)-protected fluorescent gold nanoclusters (BSA-AuNCs) have been fabricated and applied in various fields. However, the conventional synthesis methods for BSA-AuNCs usually yield a low photoluminescence quantum yield (PLQY) in solution. In this study, we systematically examined the influences of incubation time, temperature, and pH on the formation process of BSA-AuNCs and then developed a novel strategy to synthesize BSA-AuNCs with PLQY (26%), far exceeding that of existing counterparts. Of the three important factors, pH, temperature, and time, pH plays a key role in the formation of BSA-AuNCs with different compositions and fluorescence properties. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) results showed that BSA-AuNCs with high purity can be produced at a pH value of 10 and the correct combination of incubation temperature and reaction time. The advantages of the obtained BSA-AuNCs, including small size, high PLQY, long lifetime, high purity, as well as facile modification, make them ideal candidates for luminescent probes in imaging and sensing applications.
Quan-Quan Zhuang, Shao-Bin He, Kai-Yuan Huang, Hua-Ping Peng, Chang-Mai Chen, Hao-Hua Deng, Xing-Hua Xia, Wei Chen, Guo-Lin Hong

2391 related Products with: Decisive role of pH in synthesis of high purity fluorescent BSA-Au nanoclusters.

2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box

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#32480260   2020/05/23 To Up

Improving the functional properties of bovine serum albumin-glucose conjugates in natural deep eutectic solvents.

Glycation between target proteins and saccharides is time-consuming or requires high temperatures. Here, a promising reaction medium, natural deep eutectic solvents (NADES), for glucose glycation with bovine serum albumin (BSA) was applied to improve the grafting of glucose-glycated BSA by shifting reaction equilibrium. Two types of glucose-glycated BSA products were prepared using NADES and water systems. SDS-PAGE and MALDI-TOF-MS revealed that BSA and glucose were covalently bonded. Compared with in water system, glycated BSA products in NADES system had more -OH groups, more disordered secondary structures, lower intrinsic fluorescence intensity, and higher ultraviolet-visible absorption. Lower surface hydrophobicity (1100 versus 1356), higher emulsifying activity index (66.17 versus 46.49 m/g), higher emulsion stability index (79.62 versus 63.61%), and lesser free sulfhydryl (8.07 versus 8.98 μmol/g) groups were obtained with NADES system than with water system. The results suggest that NADES is a suitable alternative reaction medium for promoting the glycation of BSA.
Jing-Jing Fu, Cong Sun, Xian-Bing Xu, Da-Yong Zhou, Liang Song, Bei-Wei Zhu

1769 related Products with: Improving the functional properties of bovine serum albumin-glucose conjugates in natural deep eutectic solvents.

100 ml500 g Product tipe: Kit100ug500 ml1 ml50 ml200ul500 ml10 ml96T100 μg100ul

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#32480063   2020/05/23 To Up

Dynamic replacement of H3.3 affects nuclear reprogramming in early bovine SCNT embryos.

The histone variant H3.3 is an important maternal factor in fertilization of oocytes and reprogramming of somatic cell nuclear transfer (SCNT) embryos. As a crucial replacement histone, maternal H3.3 is involved in chromatin remodeling and zygote genome activation. Litte is, however, known about the replacement of H3.3 in the bovine SCNT embryos. In this study, the maternal H3.3 in mature ooplasm was labeled with HA tag and the donor cells H3.3 was labeled with Flag tag, in order to observe the replacement of H3.3 in the bovine SCNT embryos. Meanwhile, maternal H3.3 knockdown was performed by microinjecting two different interfering fragments before nucleus transfer. It was showed that the dynamic replacement between maternal- and donor nucleus-derived H3.3 was detected after SCNT. And it could be observed that the blastocyst development rate of the cloned embryos decreased from 22.3% to 8.2-10.3% (P < 0.05), the expression of Pou5f1 and Sox2 was down-regulated and the level of H3K9me3 was increased in the interfered embryos. In summary, H3.3 replacement impacted on the process of reprogramming, including embryonic development potential, activation of pluripotency genes and epigenetic modification in bovine SCNT embryos.
Yile Wang, Yanhe Li, Deji Luan, Jian Kang, Rongjun He, Yong Zhang, Fusheng Quan

1496 related Products with: Dynamic replacement of H3.3 affects nuclear reprogramming in early bovine SCNT embryos.

25 mg100ug Lyophilized10mg500 ml100ug Lyophilized35mg500 ml2 Pieces/Box

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#32480006   2020/05/22 To Up

Integrated farming system producing zero emissions and sustainable livelihood for small-scale cattle farms: Case study in the Mekong Delta, Vietnam.

This study proposes an integrated cattle breeding and cultivation system that provides zero emission and sustainable livelihood for the community in rural areas. The proposed integrated farming system improves agricultural productivity and environmental and sanitation conditions, minimizes the amount of waste, and increases the family income up to 41.55%. Several waste types can be recycled and transformed into valuable products, such as energy for cooking, organic fertilizer for crops, and cattle feed for breeding. Wastewater effluent from the biogas tank can be treated by biochar and results show that it then meets the standards for irrigation purposes. Also, the waste flow from cattle breeding supplies enough nutrients to cultivate plants, and the plants grown supply are adequate food for the 30 cows living on the farm. This research shows that the use of an integrated farming system could achieve zero-emission goal. Thereby, it provides a sustainable livelihood for cattle breeding family farms. The proposed integrated cattle breeding and cultivation system improves agricultural productivity, environmental and increases the farmer income up to 41.55%.
Le Thanh Hai, Quoc Ba Tran, Van Tung Tra, Thi Phuong Thao Nguyen, Trong Nhan Le, Hans Schnitzer, Gerhart Braunegg, Son Le, Cong Tin Hoang, Xuan Cuong Nguyen, Van-Huy Nguyen, Wanxi Peng, Soo Young Kim, Su Shiung Lam, Quyet Van Le

1192 related Products with: Integrated farming system producing zero emissions and sustainable livelihood for small-scale cattle farms: Case study in the Mekong Delta, Vietnam.

100 μg

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#32479937   2020/05/29 To Up

Influence of succinylation of a wide-pore albumin cryogels on their properties, structure, biodegradability, and release dynamics of dioxidine loaded in such spongy carriers.

The goal of this study was to reveal how the chemical modification, succinylation in this case, of the wide-pore serum-albumin-based cryogels affects on their osmotic characteristics (swelling extent), biodegradability and ability to be loaded with the bactericide substance - dioxidine, as well as on its release. The cryogels were prepared via the cryogenic processing (freezing - frozen storage - thawing) of aqueous solutions containing bovine serum albumin (50 g/L), denaturant (urea or guanidine hydrochloride, 1.0 mol/L) and reductant (cysteine, 0.01 mol/L). Freezing/frozen storage temperatures were either -15, or -20, or -25 °C. After defrosting, spongy cryogels were obtained that possessed the system of interconnected gross pores, whose shape and dimensions were dependent on the freezing temperature and on the type of denaturant introduced in the feed solution. Subsequent succinylation of the resultant cryogels caused the growth of the swelling degree of the pore walls of these spongy materials, resulted in strengthening of their resistance against of trypsinolysis and gave rise to an increase in their loading capacity with respect to dioxidine. With that, the microbiological tests showed a higher bactericidal activity of the dioxidine-loaded sponges based on the succinylated albumin cryogels as compared to that of the drug-carriers based on the non-modified protein sponges.
Vladimir I Lozinsky, Anastasiya O Shchekoltsova, Ekaterina S Sinitskaya, Olga I Vernaya, Anastasiya V Nuzhdina, Irina V Bakeeva, Mariam G Ezernitskaya, Alexander M Semenov, Tatyana I Shabatina, Mikhail Ya Melnikov

2117 related Products with: Influence of succinylation of a wide-pore albumin cryogels on their properties, structure, biodegradability, and release dynamics of dioxidine loaded in such spongy carriers.

5 G100ug 0.1 mg 100 50 4 Membranes/Box100ug Lyophilized100 μg100 μg

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#32479874   2020/05/29 To Up

Computational Fluid Dynamics Modeling of Proximal Landing Zones for Thoracic Endovascular Aortic Repair in the Bovine Arch Variant.

To assess the endograft displacement forces (DF), which quantify the forces exerted by the pulsatile blood flow on the vessel wall and transmitted on the terminal fixation site of the endograft after its deployment, in proximal landing zones (PLZs) of the bovine aortic arch variant.
Massimiliano M Marrocco-Trischitta, Rodrigo M Romarowski, Moad Alaidroos, Francesco Sturla, Mattia Glauber, Giovanni Nano

2166 related Products with: Computational Fluid Dynamics Modeling of Proximal Landing Zones for Thoracic Endovascular Aortic Repair in the Bovine Arch Variant.

100 ml 5 G 1 G10 ml100 ml50 ml

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