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Search results for: CD326

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#32665078   2020/06/20 To Up

Circulating extracellular vesicles as non-invasive biomarker of rejection in heart transplant.

Circulating extracellular vesicles (EVs) are raising considerable interest as a non-invasive diagnostic tool, as they are easily detectable in biologic fluids and contain a specific set of nucleic acids, proteins, and lipids reflecting pathophysiologic conditions. We aimed to investigate differences in plasma-derived EV surface protein profiles as a biomarker to be used in combination with endomyocardial biopsies (EMBs) for the diagnosis of allograft rejection.
Chiara Castellani, Jacopo Burrello, Marny Fedrigo, Alessio Burrello, Sara Bolis, Dario Di Silvestre, Francesco Tona, Tomaso Bottio, Vanessa Biemmi, Giuseppe Toscano, Gino Gerosa, Gaetano Thiene, Cristina Basso, Sarah L Longnus, Giuseppe Vassalli, Annalisa Angelini, Lucio Barile

1440 related Products with: Circulating extracellular vesicles as non-invasive biomarker of rejection in heart transplant.

1 kit96 tests100 μg900 tests96 samples100 assays100 assays

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#32645550   2020/07/06 To Up

SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection.

Suppressor of cytokine signaling 3 (SOCS3) is a negative regulator of TBK1 and interferon pathway and the expression of SOCS3 is closely correlated with symptoms of influenza patients. However, whether deletion of Socs3 in the lung epithelial cells would affect influenza lung replication and inflammation in vivo is unknown. To test this, we approached the influenza infected Socs3 and SpcCre.Socs3 mice. We first found that knockdown of Socs3 in lung epithelial cells reduced influenza replication. However, in the in vivo study, there was a reduction of SOCS3 in the influenza-infected neutrophils coincided with an increase of SOCS3 in the CD45CD326 lung epithelial cells in PR8-infected SpcCre.Socs3 mice. SOCS3-deficient neutrophils expressed higher levels of IL-17 that enhanced chemokine expression in the lung epithelial cells. Lung SOCS3-dificient epithelial cells increased expression of GM-CSF and PGE which promoted SpcCre.Socs3 neutrophils to yield SOCS3. SpcCre.Socs3 lung epithelial cells internalized SOCS3 released from GM-CSF + PGE-stimulated SpcCre.Socs3 neutrophils, which could boost influenza replication in the lung epithelial cells. Thus, in the in vivo study, deletion of SOCS3 from lung epithelium could be nullified by the uptake from SOCS3 from infiltrated neutrophils. In addition, deletion of Socs3 from myeloid cells reduced lung influenza infection, but increased lung inflammation. Taken together, deletion of SOCS3 could suppress influenza replication, but intracellular SOCS3 communication between neutrophils and lung epithelial cells confounds this effect.
Ling Li, Haiya Wu, Qingmei Li, Jie Chen, Kaifeng Xu, Jinfu Xu, Xiao Su

2884 related Products with: SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection.

100 μg250ul

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#32614947   2020/07/02 To Up

Single cell analyses and machine learning define hematopoietic progenitor and HSC-like cells derived from human PSCs.

Haematopoietic stem and progenitor cells (HSPCs) develop through distinct waves at various anatomical sites during embryonic development. The in vitro differentiation of human pluripotent stem cells (hPSCs) is able to recapitulate some of these processes, however, it has proven difficult to generate functional haematopoietic stem cells (HSCs). To define the dynamics and heterogeneity of HSPCs that can be generated in vitro from hPSCs, we exploited single cell RNA sequencing (scRNAseq) in combination with single cell protein expression analysis. Bioinformatics analyses and functional validation defined the transcriptomes of naïve progenitors as well as erythroid, megakaryocyte and leukocyte-committed progenitors and we identified CD44, CD326, ICAM2/CD9 and CD18 as markers of these progenitors, respectively. Using an artificial neural network (ANN), that we trained on a scRNAseq derived from human fetal liver, we were able to identify a wide range of hPSCs-derived HPSC phenotypes, including a small group classified as HSCs. This transient HSC-like population decreased as differentiation proceeded and was completely missing in the dataset that had been generated using cells selected on the basis of CD43expression. By comparing the single cell transcriptome of in vitro-generated HSC-like cells with those generated within the fetal liver we identified transcription factors and molecular pathways that can be exploited in the future to improve the in vitro production of HSCs.
Antonella Fidanza, Patrick Simon Stumpf, Prakash Ramachandran, Sara Tamagno, Ann Babtie, Martha Lopez-Yrigoyen, Alice Helen Taylor, Jennifer Easterbrook, Beth Henderson, Richard Axton, Neil Cowan Henderson, Alexander Medvinsky, Katrin Ottersbach, Nicola Romanò, Lesley M Forrester

1720 related Products with: Single cell analyses and machine learning define hematopoietic progenitor and HSC-like cells derived from human PSCs.

0.1ml (1mg/ml)1 mg1.00 flask4 x 96-well plate1.00 flask1025 1.00 flask1.00 flask200 2ug0.1 mg

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#32361317   2020/04/12 To Up

SERS studies on normal epithelial and cancer cells derived from clinical breast cancer specimens.

Surface-enhanced Raman scattering (SERS) spectroscopy of single-cell suspensions obtained from fresh specimens of breast cancer tissue and normal breast tissue by mechanical enzymatic digestion was obtained and analysed, which is different from most Raman studies using breast cancer cell lines. Random forest classification was implemented to develop effective diagnostic algorithms for the classification of SERS of different typed cells. We first examined the SERS spectra of the primary breast cancer single cell and normal epithelial single cell obtained by flow sorting cytometry due to their biomarkers of CD326+/CD45-. Comparison analyses on their SERS spectra disclose that the nucleic acid and protein levels of the primary breast cancer single cell are higher than those of the normal epithelial single cell, while the lipids are at a relatively lower level. An important finding is that the cholesterol, palmitic acid, and sphingomyelin in the cancer cell profiles exhibit stronger than those of normal cells, while the glycans are at a relatively lower level. Furthermore, the standard deviation (SD) of the normal epithelial single cell is larger than that of the breast cancer cell, and the SD of the primary breast cancer single cell is more obvious than that of the normal epithelial cells. In addition, the prospective application of an algorithm to the dataset results in an accuracy of 78.2%, a precision of 75.5%, and a recall of 66.7%. The breast cancer diagnostic model laid a solid foundation for judgment of breast-conserving surgical margins and early diagnosis of breast cancer.
LiShengNan Shen, Ye Du, Na Wei, Qian Li, SiMin Li, TianMeng Sun, Shuping Xu, Han Wang, XiaXia Man, Bing Han

1469 related Products with: SERS studies on normal epithelial and cancer cells derived from clinical breast cancer specimens.



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#32158188   2020/01/23 To Up

In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment.

Colorectal cancer (CRC) is a type of cancer in humans that leads to high mortality and morbidity. CD166 and CD326 are immunoglobulins that are associated with cell migration. These molecules are included in tumorigenesis of CRC and serve a great marker of CRC stem cells. In the present study, we devised a novel chimeric protein including the V-domain of the CD166 and two epitopes of CD326 to use in diagnostic or therapeutic applications.
Hassan Dana, Ghanbar Mahmoodi Chalbatani, Elahe Gharagouzloo, Seyed Rouhollah Miri, Fereidoon Memari, Reza Rasoolzadeh, Mohammad Reza Zinatizadeh, Peyman Kheirandish Zarandi, Vahid Marmari

1650 related Products with: In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment.

1 Set1 Set1 Set1 Set1001 Set1 Set1 Set

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#32102433   2020/02/24 To Up

Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease.

Disruption of epithelial junctional complex (EJC), especially tight junctions (TJ), resulting in increased intestinal permeability, is supposed to activate the enhanced immune response to gluten and to induce the development of celiac disease (CD). This study is aimed to present the role of EJC in CD pathogenesis. To analyze differentially expressed genes the next-generation mRNA sequencing data from CD326+ epithelial cells isolated from non-celiac and celiac patients were involved. Ultrastructural studies with morphometry of EJC were done in potential CD, newly recognized active CD, and non-celiac controls. The transcriptional analysis suggested disturbances of epithelium and the most significant gene ontology enriched terms in epithelial cells from CD patients related to the plasma membrane, extracellular exome, extracellular region, and extracellular space. Ultrastructural analyses showed significantly tighter TJ, anomalies in desmosomes, dilatations of intercellular space, and shorter microvilli in potential and active CD compared to controls. Enterocytes of fetal-like type and significantly wider adherence junctions were observed only in active CD. In conclusion, the results do not support the hypothesis that an increased passage of gluten peptides by unsealing TJ precedes CD development. However, increased intestinal permeability due to abnormality of epithelium might play a role in CD onset.
Agnieszka Sowińska, Yasser Morsy, Elżbieta Czarnowska, Beata Oralewska, Ewa Konopka, Marek Woynarowski, Sylwia Szymańska, Maria Ejmont, Michael Scharl, Joanna B Bierła, Marcin Wawrzyniak, Bożena Cukrowska

2601 related Products with: Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease.

96 tests96 tests

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#32070615   2019/12/24 To Up

Multiplex detection and characterization of breast cancer exosomes by magneto-actuated immunoassay.

The exosomes are emerging as biomarkers for the detection of cancer in early stages, as well as for the follow-up of the patients under treatment. This paper describes the characterization of exosomes derived from three different breast cancer cell lines (MCF7, MDA-MB-231 and SKBR3), and the quantification based on a magneto-actuated immunoassay. The exosomes are separated and preconcentrated on magnetic particles by immunomagnetic separation and labelled with a second antibody conjugated with an enzyme for the optical readout performed with a standard microplate reader. Several molecular biomarkers, including the general tetraspanin CD9, CD63 and CD81, and the receptors related with cancer (CD24, CD44, CD54, CD326 and CD340) were studied either for the immunomagnetic separation or the labelling, in different formats. After a rational selection of the biomarkers, this immunoassay is able to detect 10 exosomes μL directly in human serum without any treatment, such as ultracentrifugation. The interference from free receptors in the samples could easily be prevented by performing the immunomagnetic separation with antiCD81 modified magnetic particles and the labeling based on either CD24 or CD340. Furthermore, the differentiation of healthy donors and breast cancer individuals was also demonstrated. This approach is a highly suitable alternative method for flow cytometry, providing a sensitive method for the multiplex detection but using instrumentation widely available in resource-constrained laboratories and requiring low-maintenance, as is the case of a microplate reader operated by filters.
Silio Lima Moura, Carme García Martín, Mercè Martí, María Isabel Pividori

2601 related Products with: Multiplex detection and characterization of breast cancer exosomes by magneto-actuated immunoassay.

96T

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#32010426   2020/01/14 To Up

Deregulation of cancer-stem-cell-associated miRNAs in tissues and sera of colorectal cancer patients.

Colorectal cancer (CRC) is a deadly tumour in Western countries characterized by high cellular/molecular heterogeneity. Cancer stem cells (CSC) act in cancer recurrence, drug-resistance and in metastatic epithelial-to-mesenchymal transition. microRNAs (miRNAs) contribute to cancer is increasing, and miRNA roles in CSC phenotype and fate and their utility as CRC biomarkers have also been reported. Here, we investigated miR-21, miR-221, miR-18a, miR-210, miR-31, miR-34a, miR-10b and miR-16 expression in experimental ALDH and CD44/CD326 colorectal CSCs obtained from the human CRC cell lines HCT-116, HT-29 and T-84. Then, we moved our analysis in cancer tissue (CT), healthy tissue (HT) and serum (S) of adult CRC patients (n=12), determining relationships with clinical parameters (age, sex, metastasis, biochemical serum markers). Specific miRNA patterns were evident (normal, monolayers and CSCs) and in patients' samples stratified by TNM stage (LOW vs HIGH) or metastasis (Met vs no-Met). miR-21, miR-210, miR-34a upregulation ad miR-16 dowregulation associated with the CSCs phenotype. miR-31b robustly overexpressed in monolayers and CSCs, and in CT ad S of HIGH grade and Met patients, suggesting a role as marker of CRC progression and metastasis. miR-18a upregulated in all cancer models and associated to CSC phenotype, and to metastasis and age in patients. miR-10b downregulated in CT and S of LOW/HIGH grade and no-Met patients. Our results identify miRNAs useful as colorectal CSC biomarker and that miR-21, miR-210, miR-10b and miR-31b are promising markers of CRC. A specific role of miR-18a as metastatic CRC serum biomarker in adult patients was also highlighted.
Cristiano Farace, Andrea Pisano, Carmen Griñan-Lison, Giuliana Solinas, Gema Jiménez, Marina Serra, Esmeralda Carrillo, Fabrizio Scognamillo, Federico Attene, Andrea Montella, Juan Antonio Marchal, Roberto Madeddu

2124 related Products with: Deregulation of cancer-stem-cell-associated miRNAs in tissues and sera of colorectal cancer patients.



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#31970137   2019/02/08 To Up

Initial detection of circulating tumor cells from metastatic prostate cancer patients with a novel small device.

Various devices for isolating and detecting circulating tumor cells (CTCs) have been developed, whereas the CellSearch® system has been clinically used in numerous prostate CTC studies. CTCs might become more useful surrogate markers of prostate cancer, and they should be measured in all settings, but a smaller, low-cost CTC capture system is required.
Kotaro Obayashi, Jun Akatsuka, Yuki Endo, Hayato Takeda, Tatsuro Hayashi, Yuka Toyama, Yasutomo Suzuki, Tsutomu Hamasaki, Go Kimura, Takashi Ohnaga, Yukihiro Kondo

1259 related Products with: Initial detection of circulating tumor cells from metastatic prostate cancer patients with a novel small device.



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