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Search results for: CEA

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#32272156   2020/04/06 To Up

Development of blood-based biomarker tests for early detection of colorectal neoplasia: Influence of blood collection timing and handling procedures.

Blood-based, cancer-associated biomarkers are susceptible to a variety of well-known preanalytical factors. The influence of bowel preparation before a diagnostic colonoscopy on biomarker levels is, however, poorly investigated. The present study assessed the influence of bowel preparation on colorectal cancer-associated biomarkers. In addition, the effect of single versus double centrifugation of plasma biomarkers was assessed.
Niels Lech Pedersen, Mathias Mertz Petersen, Jon J Ladd, Paul D Lampe, Robert S Bresalier, Gerard J Davis, Christina Demuth, Sarah Ø Jensen, Claus L Andersen, Linnea Ferm, Ib Jarle Christensen, Hans J Nielsen

2113 related Products with: Development of blood-based biomarker tests for early detection of colorectal neoplasia: Influence of blood collection timing and handling procedures.

500 tests132/kit 5 G96 tests100 Tests500 tests66/kit50ml

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#32271158   2020/04/08 To Up

Tumor-associated exosomal miRNA biomarkers to differentiate metastatic vs. nonmetastatic non-small cell lung cancer.

Background Exosomal microRNAs (miRNAs) are proposed to be excellent candidate biomarkers for clinical applications. However, little is known about their potential value as diagnostic biomarkers for metastatic non-small cell lung cancer (NSCLC). Methods In this study, microarrays were used to determine distinct miRNA profiles of plasma exosomes in a discovery cohort of healthy donors, metastatic NSCLC and nonmetastatic NSCLC patients. Three potential candidate miRNAs were selected based on the differential expression profiles. The discovery set data were validated by quantitative real-time polymerase chain reaction using a validation cohort. Results NSCLC patients (n = 80) and healthy controls (n = 30) had different exosome-related miRNA profiles in plasma. Results demonstrated that the level of let-7f-5p was decreased in plasma exosomes of NSCLC patients (p < 0.0001). Further analysis of three differentially expressed miRNAs revealed that miR-320a, miR-622 and let-7f-5p levels could significantly segregate patients with metastatic NSCLC from patients with nonmetastatic NSCLC (p < 0.0001, p < 0.0001 and p = 0.023, respectively). In addition, the combination of let-7f-5p, CEA and Cyfra21-1 generated an area under the curve (AUC) of 0.981 for the diagnosis of NSCLC patients, and the combination of miR-320a, miR-622, CEA and Cyfra21-1 had an AUC of 0.900 for the diagnosis of patients with metastatic NSCLC. Conclusions This novel study demonstrated that plasma exosomal miRNAs are promising noninvasive diagnostic biomarkers for metastatic NSCLC.
Ning Wang, Wei Guo, Xingguo Song, Lisheng Liu, Limin Niu, Xianrang Song, Li Xie

2442 related Products with: Tumor-associated exosomal miRNA biomarkers to differentiate metastatic vs. nonmetastatic non-small cell lung cancer.



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#32270773   2020/04/06 To Up

Serum mucin 3A as a potential biomarker for extrahepatic cholangiocarcinoma.

The aim of this study is to evaluate serum mucin 3A (MUC3A) as a candidate biomarker for extrahepatic cholangiocarcinoma (EHCC).
Jing Wang, Haibin Zhou, Yucheng Wang, Haitao Huang, Jing Yang, Weigang Gu, Xiaofeng Zhang, Jianfeng Yang

2306 related Products with: Serum mucin 3A as a potential biomarker for extrahepatic cholangiocarcinoma.

100 assays10 plates1ml1 kit100 plates50 assays96 Tests1 kit10 plates100Tests

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#32270338   2020/04/08 To Up

Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and ordered mesoporous carbon.

An electrochemical immunoassay for the carcinoembryonic antigen is described. It is based on the use of Au NPs modified zeolitic imidazolate framework (ZIF-8) and ordered mesoporous carbon (OMC). Au [email protected] was synthesized by reduction of chloroauric acid. It serves as immobilization support nanocarrier to increase antibody loading due to its large surface area. OMC was dropped on a glassy carbon electrode to improve electrochemical signals due to enhanced electrical conductivity. Differential pulse voltammetry was carried out to record electrochemical responses (best measured at 0.26 V vs. Ag/AgCl). The immunosensor demonstrated excellent electrochemical performance with a linear determination range of 5 pg mL to 400 ng mL and a determination limit of 1.3 pg mL (S/N = 3). The sensor also exhibited high selectivity, good stability, and acceptable reproducibility. Graphical abstract Scheme 1 Schematic representation of fabrication of the immunosensor for CEA determination.
Yingcong Zhang, Ze Zhang, Shengzhong Rong, Hongwei Yu, Hongmin Gao, Ping Ding, Dong Chang, Hongzhi Pan

1990 related Products with: Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and ordered mesoporous carbon.

1 kit(96 Wells)0.2ml (105g/ml)2 mL2 mL0.1ml (1mg/ml)2 mL1 mg0.2 mg100 µg200 50ul

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#32270227   2020/04/08 To Up

High-throughput synthetic rescue for exhaustive characterization of suppressor mutations in human genes.

Inherited or acquired mutations can lead to pathological outcomes. However, in a process defined as synthetic rescue, phenotypic outcome created by primary mutation is alleviated by suppressor mutations. An exhaustive characterization of these mutations in humans is extremely valuable to better comprehend why patients carrying the same detrimental mutation exhibit different pathological outcomes or different responses to treatment. Here, we first review all known suppressor mutations' mechanisms characterized by genetic screens on model species like yeast or flies. However, human suppressor mutations are scarce, despite some being discovered based on orthologue genes. Because of recent advances in high-throughput screening, developing an inventory of human suppressor mutations for pathological processes seems achievable. In addition, we review several screening methods for suppressor mutations in cultured human cells through knock-out, knock-down or random mutagenesis screens on large scale. We provide examples of studies published over the past years that opened new therapeutic avenues, particularly in oncology.
Farah Kobaisi, Nour Fayyad, Eric Sulpice, Bassam Badran, Hussein Fayyad-Kazan, Walid Rachidi, Xavier Gidrol

1242 related Products with: High-throughput synthetic rescue for exhaustive characterization of suppressor mutations in human genes.

96 wells (1 kit)96T96 wells (1 kit)96 wells (1 kit)10 96 wells (1 kit)96 wells (1 kit)100 1mg25 100 μg100 μg

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#32270045   2020/04/01 To Up

Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly.

Many viruses are known to form cellular compartments, also called viral factories. Paramyxoviruses, including measles virus, colocalize their proteomic and genomic material in puncta in infected cells. We demonstrate that purified nucleoproteins (N) and phosphoproteins (P) of measles virus form liquid-like membraneless organelles upon mixing in vitro. We identify weak interactions involving intrinsically disordered domains of N and P that are implicated in this process, one of which is essential for phase separation. Fluorescence allows us to follow the modulation of the dynamics of N and P upon droplet formation, while NMR is used to investigate the thermodynamics of this process. RNA colocalizes to droplets, where it triggers assembly of N protomers into nucleocapsid-like particles that encapsidate the RNA. The rate of encapsidation within droplets is enhanced compared to the dilute phase, revealing one of the roles of liquid-liquid phase separation in measles virus replication.
Serafima Guseva, Sigrid Milles, Malene Ringkjøbing Jensen, Nicola Salvi, Jean-Philippe Kleman, Damien Maurin, Rob W H Ruigrok, Martin Blackledge

2436 related Products with: Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly.

1001 g1000100 ug5001000100 mg100 5001000 100500

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#32270043   2020/04/01 To Up

MAP6 is an intraluminal protein that induces neuronal microtubules to coil.

Neuronal activities depend heavily on microtubules, which shape neuronal processes and transport myriad molecules within them. Although constantly remodeled through growth and shrinkage events, neuronal microtubules must be sufficiently stable to maintain nervous system wiring. This stability is somehow maintained by various microtubule-associated proteins (MAPs), but little is known about how these proteins work. Here, we show that MAP6, previously known to confer cold stability to microtubules, promotes growth. More unexpectedly, MAP6 localizes in the lumen of microtubules, induces the microtubules to coil into a left-handed helix, and forms apertures in the lattice, likely to relieve mechanical stress. These features have not been seen in microtubules before and could play roles in maintaining axonal width or providing flexibility in the face of compressive forces during development.
Camille Cuveillier, Julie Delaroche, Maxime Seggio, Sylvie Gory-Fauré, Christophe Bosc, Eric Denarier, Maria Bacia, Guy Schoehn, Hervé Mohrbach, Igor Kulić, Annie Andrieux, Isabelle Arnal, Christian Delphin

1850 related Products with: MAP6 is an intraluminal protein that induces neuronal microtubules to coil.

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#32270031   2020/04/01 To Up

Virus-host coexistence in phytoplankton through the genomic lens.

Virus-microbe interactions in the ocean are commonly described by "boom and bust" dynamics, whereby a numerically dominant microorganism is lysed and replaced by a virus-resistant one. Here, we isolated a microalga strain and its infective dsDNA virus whose dynamics are characterized instead by parallel growth of both the microalga and the virus. Experimental evolution of clonal lines revealed that this viral production originates from the lysis of a minority of virus-susceptible cells, which are regenerated from resistant cells. Whole-genome sequencing demonstrated that this resistant-susceptible switch involved a large deletion on one chromosome. Mathematical modeling explained how the switch maintains stable microalga-virus population dynamics consistent with their observed growth pattern. Comparative genomics confirmed an ancient origin of this "accordion" chromosome despite a lack of sequence conservation. Together, our results show how dynamic genomic rearrangements may account for a previously overlooked coexistence mechanism in microalgae-virus interactions.
Sheree Yau, Marc Krasovec, L Felipe Benites, Stephane Rombauts, Mathieu Groussin, Emmelien Vancaester, Jean-Marc Aury, Evelyne Derelle, Yves Desdevises, Marie-Line Escande, Nigel Grimsley, Julie Guy, Hervé Moreau, Sophie Sanchez-Brosseau, Yves van de Peer, Klaas Vandepoele, Sebastien Gourbiere, Gwenael Piganeau

1635 related Products with: Virus-host coexistence in phytoplankton through the genomic lens.

1mg200ul250.1ml0.25 mg1 mg10 1 mL2 0.1ml250 ug

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#32269928   // To Up

Combination diagnosis with elastography strain ratio and molecular markers effectively improves the diagnosis rate of small breast cancer and lymph node metastasis.

To evaluate the strain ratio (SR) combined with molecular pathological and serum markers for the diagnosis of breast masses.
Yi Hao, Guanghui Ren, Wei Yang, Wenyi Zheng, Yuming Wu, WenJing Li, Xin Li, Yingjia Li, Xia Guo

1656 related Products with: Combination diagnosis with elastography strain ratio and molecular markers effectively improves the diagnosis rate of small breast cancer and lymph node metastasis.



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#32269582   2020/03/25 To Up

Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in .

Photosynthesis is an essential pathway providing the chemical energy and reducing equivalents that sustain higher plant metabolism. It relies on sunlight, which is an inconstant source of energy that fluctuates in both intensity and spectrum. The fine and rapid tuning of the photosynthetic apparatus is essential to cope with changing light conditions and increase plant fitness. Recently PROTON GRADIENT REGULATION 6 (PGR6-ABC1K1), an atypical plastoglobule-associated kinase, was shown to regulate a new mechanism of light response by controlling the homeostasis of photoactive plastoquinone (PQ). PQ is a crucial electron carrier existing as a free neutral lipid in the photosynthetic thylakoid membrane. Perturbed homeostasis of PQ impairs photosynthesis and plant acclimation to high light. Here we show that a homologous kinase, ABC1K3, which like PGR6-ABC1K1 is associated with plastoglobules, also contributes to the homeostasis of the photoactive PQ pool. Contrary to PGR6-ABC1K1, ABC1K3 disfavors PQ availability for photosynthetic electron transport. In fact, in the double mutant the () the photosynthetic defect seen in the mutant is mitigated. However, the PQ concentration in the photoactive pool of the double mutant is comparable to that of mutant. An increase of the PQ mobility, inferred from the kinetics of its oxidation in dark, contributes to the mitigation of the () photosynthetic defect. Our results also demonstrate that ABC1K3 contributes to the regulation of other mechanisms involved in the adaptation of the photosynthetic apparatus to changes in light quality and intensity such as the induction of thermal dissipation and state transitions. Overall, we suggests that, besides the absolute concentration of PQ, its mobility and exchange between storage and active pools are critical for light acclimation in plants.
Thibaut Pralon, Joy Collombat, Rosa Pipitone, Brigitte Ksas, Venkatasalam Shanmugabalaji, Michel Havaux, Giovanni Finazzi, Paolo Longoni, Felix Kessler

1019 related Products with: Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in .

111115mg

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