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#33949387   // To Up

Enhanced electrochemiluminescence of CdS quantum dots capped with mercaptopropionic acid activated by EDC for Zika virus detection.

Enhanced electrochemiluminescence (ECL) signals of CdS quantum dots capped with 3-mercaptopropionic acid ([email protected] QDs) have been observed after using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) to activate the carboxyl groups. The generated ECL signals are strong enough that their images can be captured using a Huawei mobile phone. A possible mechanism for the generation of enhanced ECL signals has been proposed. Then, a sandwich immunosensor platform for detecting Zika virus (ZIKV) was fabricated with silica microspheres as the carrier and [email protected] QDs as ECL signal labels. Due to the dual signal amplification of EDC activation and microsphere enrichment, good linearity from 1.0 fg mL-1 to 1.0 ng mL-1 was exhibited by the QD-based ECL immunosensor for ZIKV detection. The detection limit was 0.3 fg mL-1.
Hui-Jun Zhang, Jin Zhu, Ning Bao, Shou-Nian Ding

2493 related Products with: Enhanced electrochemiluminescence of CdS quantum dots capped with mercaptopropionic acid activated by EDC for Zika virus detection.

0.25 mg100tests 1 G2x96 well plate100tests10 mg5 g 1 G50 assays100tests 100 G

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#33945254   2021/05/04 To Up

A Multitargeted Electrochemiluminescent Biosensor Coupling DNAzyme with Cascading Amplification for Analyzing Myocardial miRNAs.

Several circulating miRNAs are associated with the pathogenic process of acute myocardial infarction (AMI). Thus, analyzing myocardial miRNAs in the circulatory system is important for the diagnosis and treatment of AMI, especially for early-stage diagnosis. Based on the characteristics of myocardial miRNAs, an ultrasensitive and multitargeted electrochemiluminescence (ECL) sensing platform was developed with a versatile probe that can couple DNAzyme with hybridization chain reaction amplification. The target miRNA and auxiliary chains form a circular unit that shears the versatile probe hairpin, and the products subsequently trigger cascading amplification; a long strand of dsDNA is then generated with many C-rich sequences that can undergo in situ reductions to generate ECL luminophore silver clusters. Using this strategy, three myocardial miRNAs are successfully detected with a detection limit as low as 29.6 aM (/ = 3). Notably, our method can detect myocardial miRNA groups composed of multiple related circulating miRNAs with high selectivity over interfering miRNAs in blood. This is extremely important for solving the problem of diverse and low abundance of infarct-associated miRNAs. Our strategy pioneers a new idea of miRNA detection, and given its versatility and sensitivity, it is promising for the diagnosis of multigene-regulated cardiovascular diseases.
Yudie Sun, La Fang, Zhe Zhang, Yang Yi, Shengjun Liu, Qian Chen, Jian Zhang, Cheng Zhang, Lifang He, Kui Zhang

2479 related Products with: A Multitargeted Electrochemiluminescent Biosensor Coupling DNAzyme with Cascading Amplification for Analyzing Myocardial miRNAs.

5 mg1 LITRE0.1ml (1mg/ml)2.5 mg0.25 mg1.0 mg 1 G100ug1 ml100 tests0.2 mg 100ul

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#33917921   2021/04/08 To Up

Gold-seeded Lithium Niobate Nanoparticles: Influence of Gold Surface Coverage on Second Harmonic Properties.

Hybrid nanoparticles composed of an efficient nonlinear optical core and a gold shell can enhance and tune the nonlinear optical emission thanks to the plasmonic effect. However the influence of an incomplete gold shell, i.e., isolated gold nano-islands, is still not well studied. Here LiNbO (LN) core nanoparticles of 45 nm were coated with various densities of gold nano-seeds (AuSeeds). As both LN and AuSeeds bear negative surface charge, a positively-charged polymer was first coated onto LN. The number of polymer chains per LN was evaluated at 1210 by XPS and confirmed by fluorescence titration. Then, the surface coverage percentage of AuSeeds onto LN was estimated to a maximum of 30% using ICP-AES. The addition of AuSeeds was also accompanied with surface charge reversal, the negative charge increasing with the higher amount of AuSeeds. Finally, the first hyperpolarizability decreased with the increase of AuSeeds density while depolarization values for Au-seeded LN were close to the one of bare LN, showing a predominance of the second harmonic volumic contribution.
Rachael Taitt, Mathias Urbain, Zacharie Behel, Ana-María Pablo-Sainz-Ezquerra, Iryna Kandybka, Eloïse Millet, Nicolas Martinez-Rodriguez, Christelle Yeromonahos, Sandrine Beauquis, Ronan Le Dantec, Yannick Mugnier, Pierre-François Brevet, Yann Chevolot, Virginie Monnier

2734 related Products with: Gold-seeded Lithium Niobate Nanoparticles: Influence of Gold Surface Coverage on Second Harmonic Properties.

100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized25ml625/1560 assays100ug100ug Lyophilized100ug Lyophilized 125 ml

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#33913953   // To Up

Highly efficient electrochemiluminescence resonance energy transfer material constructed from an AIEgen-based 2D ultrathin metal-organic layer for thrombin detection.

A facile strategy to design a highly efficient electrochemiluminescence resonance energy transfer (ECL-RET) system was proposed by using an AIEgen-based 2D ultrathin metal-organic layer (MOL) to coordinatively immobilize energy donors and acceptors simultaneously, in which the distance between adjacent donor-acceptor pairs was precise and short for obtaining high ECL-RET efficiency.
Yang Yang, Li-Ying Yao, Wen-Bin Liang, Wei Huang, Yong-Jiang Zhang, Jia-Ling Zhang, Ruo Yuan, Dong-Rong Xiao

2248 related Products with: Highly efficient electrochemiluminescence resonance energy transfer material constructed from an AIEgen-based 2D ultrathin metal-organic layer for thrombin detection.

100ug4 Arrays/Slide2x96 well plate1 Kit100ug Lyophilized100ug100ug Lyophilized4 Membranes/Box2 Pieces/Box100ug96 Tests

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#33908636   2021/04/28 To Up

Vedolizumab Immunogenicity With Long-Term or Interrupted Treatment of Patients With Inflammatory Bowel Disease.

Patients in GEMINI 1 or 2 (NCT00790933; Eudra CT2008-002784-14) with ulcerative colitis or Crohn's disease had low immunogenicity rates after vedolizumab treatment for up to 52 weeks. We report immunogenicity rates from the GEMINI long-term safety (LTS) study using a new drug-tolerant electrochemiluminescence (ECL) assay, including analyses in patients who received continuous vedolizumab induction and maintenance in GEMINI 1 or 2 and LTS, or vedolizumab induction and placebo maintenance in GEMINI 1 or 2 then retreatment in LTS (treatment interruption). Patients were enrolled in GEMINI LTS from GEMINI 1, 2, or 3, or as de novo vedolizumab-treated patients; all received vedolizumab 300 mg intravenously every 4 weeks. Vedolizumab antidrug antibody (ADA) status was determined by ECL assay; ADA-positive samples were characterized by neutralizing activity. Vedolizumab ADA data were available for 1753 patients: 1513 continuously treated with vedolizumab before/during GEMINI LTS, 240 retreated after treatment interruption. Among continuously treated patients, 36 (2.4%) were ADA-positive (15 persistently, 20 neutralizing ADA-positive). Among retreated patients, 53 (22.1%) were ADA-positive (42 persistently, 40 neutralizing ADA-positive). Longitudinal immunogenicity rates increased during placebo maintenance (19.4% at week 52) then decreased in GEMINI LTS to rates (0 at the final visit) similar to continuously treated patients. ADA positivity was 1.1% versus 2.5% (continuous treatment) and 23.1% versus 22.0% (retreatment) among patients with and without infusion-related reactions, respectively. Long-term vedolizumab treatment was associated with generally low immunogenicity rates; vedolizumab-retreated patients had higher rates during placebo maintenance, which decreased during retreatment. No relationship was observed between immunogenicity and infusion-related reactions. This article is protected by copyright. All rights reserved.
Timothy Wyant, Lili Yang, Richard A Lirio, Maria Rosario

2965 related Products with: Vedolizumab Immunogenicity With Long-Term or Interrupted Treatment of Patients With Inflammatory Bowel Disease.

0.1 mg100 μg100 μg100 μg100 μg100 μg100 μg100 μg

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#33894484   2021/04/20 To Up

Performance enhancement by particle gradient assembly patterning of electrochemiluminescence immunosensor formed using magnetolithgraphy in determination of human serum albumin.

Gradient properties facilitate the correlation of chemical and physical features of particles on the structure and adherent fate. Herein, performance enhancement is explored by particle gradient assembly patterning (PGAP) formed with magnetic field gradient (MFG) by magnetolithography (ML) in the electrochemiluminescence (ECL) measurement. Magnetic FeO nanoparticles were selected as nanocarriers and coated with a SiO layer for immobilization of primary antibodies. CdTe quantum dots with protein G coatings were selected as signal labels and conjugated with secondary antibodies. Magnetized 500-nm pillar, 1 μm- and 3 μm-line arrays of nickel were placed behind the working electrode modifying the sandwich-structured ECL immunosensor to form various PGAPs. A performance enhancement of ca. 2.4 times was observed when comparing the PGAP-free immunosensor to the researched gradient immunosensor, formed with a magnetized 3 μm-line array of nickel. This concludes that the sensitivity of an ECL immunosensor has been enhanced due to PGAP properties. When the immunosensor with PGAP properties was used to quantify human serum albumin, it exhibited a wide linear range (10-480 ng/mL), and a limit of detection of 10 ng/mL. PGAP properties, formed with MFG by ML, provides a simple method to improve the ECL performance.
Bao-Yu Liao, Chih-Chia Cheng, Chih-Feng Wang, Chien-Hsing Lu, Jem-Kun Chen

2851 related Products with: Performance enhancement by particle gradient assembly patterning of electrochemiluminescence immunosensor formed using magnetolithgraphy in determination of human serum albumin.

100 mg5ml100 ul1 mg100 mg50 mg10gm10 mg100 ug50 mg10 mg10 mg

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#33893832   2021/04/24 To Up

Recent advances in electrochemiluminescence luminophores.

Electrochemiluminescence (ECL) has continued to receive considerable attention in various applications, owing to its intrinsic advantages such as near-zero background response, wide dynamic range, high sensitivity, simple instrumentation, and low cost. The ECL luminophore is one of the most significant components during the light generation processes. Despite significant progress that has been made in the synthesis of new luminophores and their roles in resolving various challenges, there are few comprehensive summaries on ECL luminophores. In this review, we discuss some of the recent advances in organic, metal complexes, nanomaterials, metal oxides, and near-infrared ECL luminophores. We also emphasize their roles in tackling various challenges with illustrative examples that have been reported in the last few years. Finally, perspective and some unresolved challenges in ECL that can potentially be addressed by introducing new luminophores have also been discussed. Graphical abstract.
Abubakar Abdussalam, Guobao Xu

2236 related Products with: Recent advances in electrochemiluminescence luminophores.

10mg4 Membranes/Box0.5 mg25 mg100μg100ug Lyophilized 5 G1 Set

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