Search results for: CellQuanti-MTT™ Cell Viability Assay Kits
#33468219 
2021/01/19
To Up
Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury.
Sepsis is life-threatening disease with systemic inflammation and can lead to various diseases, including septic acute kidney injury (AKI). Recently, diverse circular RNAs (circRNAs) are considered to be involved in the development of this disease. In this study, we aimed to elucidate the role of circ-FANCA and the potential action mechanism in sepsis-induced AKI.Heyun Li, Xia Zhang, Peng Wang, Xiaoyan Zhou, Haiying Liang, Caoni Li
1928 related Products with: Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury.
96T400 ug400 ug96 assays1 mg1 mg
Related Pathways
#33413359 2021/01/07
To Up
Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells.
Norwogonin is a natural flavone with three phenolic hydroxyl groups in skeletal structure and has excellent antioxidant activity. However, the neuroprotective effect of norwogonin remains unclear. Here, we investigated the protective capacity of norwogonin against oxidative damage elicited by hypoxia in PC12 cells.Linlin Jing, Rongmin Gao, Jie Zhang, Dongmei Zhang, Jin Shao, Zhengping Jia, Huiping Ma
1651 related Products with: Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells.
100ul100 ug400 ug2 Pieces/Box10 rxns100 ul1.00 flask2 ml100ug1x10e7 cells1 mg
Related Pathways
#33398543 2021/01/05
To Up
Tadalafil Alleviates LPS-Induced Inflammation and Oxidative Stress of RWPE-1 Cell by Regulating the Akt/Nrf2 Signaling Pathway.
Tadalafil (TAD) is primarily a treatment drug for erectile dysfunction. Studies have shown that TAD has a therapeutic effect on prostatitis, but the specific mechanism has not been reported. LPS induced RWPE-1 cells to form a model of chronic nonbacterial prostatitis (CNP). Cell activity was measured by MTT assay. Apoptosis was detected by TUNEL assay. Western blot was used to detect the expression of apoptosis-related proteins Bcl-2, Bax, Caspase-3, and cleaved caspase3. ELISA was used to detect the expression of inflammatory cytokines TNF-α, IL-6, and IL-8. GSH, catalase (CAT), and malondialdehyde (MDA) kits were used to detect the expression of oxidative stress-related indicators GSH, CAT, and MDA. Western blot was used to detect the expression of proteins related to Akt/Nrf2 signaling pathway. After different concentrations of TAD were given, the survival rate of LPS-induced RWPE-1 cells decreased, apoptosis increased, and inflammation and oxidative stress decreased. This process is accompanied by the activation of the Akt/Nrf2 signaling pathway. The addition of AKT inhibitor (HY-10249A) reversed the inhibitory effect of TAD on LPS-induced inflammatory response and oxidative stress of RWPE-1 cell. TAD alleviated LPS-induced inflammation and oxidative stress of RWPE-1 cell by regulating the Akt/Nrf2 signaling pathway.Xiaoqing Song, Guodong Chen, Caixia Li, Chunyan Yang, Yun Deng
2356 related Products with: Tadalafil Alleviates LPS-Induced Inflammation and Oxidative Stress of RWPE-1 Cell by Regulating the Akt/Nrf2 Signaling Pathway.
1.5 x 10^6 cells2 Pieces/Box1.5x10(6) cells2 Pieces/Box100ul100ul100ug1.5x10(6) cells100ug Lyophilized100ug100ml100ug Lyophilized
Related Pathways
#33372599 2020/12/07
To Up
RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer.
Ferroptosis is the process of cell death triggered by lipid peroxides, and inhibition of glutathione (GSH) synthesis leads to ferroptosis. Liver cancer progression is closely linked to ferroptosis suppression. However, the mechanism by which inhibition of GSH synthesis suppresses potential ferroptosis of liver cancer cells and whether ferroptosis-related liver cancer biomarkers have a promising diagnostic value remain unknown.Yueyue Yang, Jiafei Lin, Susu Guo, Xiangfei Xue, Yikun Wang, Shiyu Qiu, Jiangtao Cui, Lifang Ma, Xiao Zhang, Jiayi Wang
2716 related Products with: RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer.
100ug Lyophilized0.25 mg1 g100ug Lyophilized
Related Pathways
#33365072 2020/11/25
To Up
Icariin enhances cell survival in lipopolysaccharide-induced synoviocytes by suppressing ferroptosis via the Xc-/GPX4 axis.
The mechanism of action of synovitis, as the vital pathological process of rheumatoid arthritis and osteoarthritis, remains to be elucidated. The effects and the mechanism of icariin (ICA), which is a promising therapeutic agent in synovitis, was investigated in the present study. In addition, ferroptosis, a vital cell process involved in several diseases, was also studied in synovitis for the first time. Lipopolysaccharide (LPS)-induced synoviocytes served as a synovitis cell model. The cells were divided into control, LPS and experimental groups and were treated with different concentrations of ICA. Cell viability was determined by Cell Counting Kit-8 assay and cell death was determined by flow cytometry. The expression levels of proteins (GPX4, SLC7A11, SLC3A2L, TRF, Nrf2 and NCOA4) were measured by western blotting. Quantification of malondialdehyde (MDA), iron and glutathione peroxidase 4 (GPX4) activity levels were performed via using corresponding assay kits. Cell death was increased, and cell viability was decreased in LPS-induced synoviocytes. Furthermore, MDA levels and iron content were elevated and GPX levels was reduced in LPS-induced synoviocytes. Transferrin receptor protein 1 and nuclear receptor coactivator 4 were upregulated and proteins of the Xc-/GPX4 axis, as well as nuclear factor erythroid 2-related factor 2, were decreased by LPS treatment. All aforementioned LPS affects were alleviated by ICA via a concentration-dependent manner. ICA counteracted the effects of RSL3, a ferroptosis activator, on cell viability, lipid peroxidation, iron content and relative protein expression of ferroptosis in synoviocytes. ICA protects the cells from death in synoviocytes induced by LPS, via the inhibition of ferroptosis by activating the Xc-/GPX4 axis, which can be exploited as a new therapeutic strategy for synovitis.Huasong Luo, Rui Zhang
2763 related Products with: Icariin enhances cell survival in lipopolysaccharide-induced synoviocytes by suppressing ferroptosis via the Xc-/GPX4 axis.
400 ug400 ug1 mg1 mg96 assays100 µg24 tests150 ul1 mL1 mg
Related Pathways
#33340723 2020/12/16
To Up
LncRNA SOX2OT Knockdown Alleviates Lipopolysaccharide-Induced Damage of PC12 Cells by Regulating miR-331-3p/Neurod1 Axis.
Long noncoding RNAs (lncRNAs) serve as crucial regulators in the pathogenesis of spinal cord injury (SCI). However, the role of lncRNA SOX2 overlapping transcript (SOX2OT) in SCI remains to be well revealed.Ronggang Li, Xiaofeng Li, Yong Huang, Haiying Qiu, Linlin Li, Zhenggang Bi
1597 related Products with: LncRNA SOX2OT Knockdown Alleviates Lipopolysaccharide-Induced Damage of PC12 Cells by Regulating miR-331-3p/Neurod1 Axis.
96 assays1.00 flask100ul96 assays 1 kit(s) 10ml1.00 flask100 µg 5 G400 ug10ml
Related Pathways
#33326961 2020/12/16
To Up
Effect of Cryptotanshinone on Measures of Rat Cardiomyocyte Oxidative Stress and Gene Activation Associated with Apoptosis.
Oxidative stress is a key factor that results in cardiomyocyte apoptosis and cardiovascular diseases. Cryptotanshinone (CTS), one of the major bioactive constitutes extracted from the root of the plant Salvia miltiorrhizaBunge, has been widely studied for various disease treatments. However, the roles of CTS on cardiomyocytes remain unclear.Gang Shi, Ying Wang, Jie Yang, Ting Liu, Fei Luo, Guoyin Jin, Yuan Ma, Yongpeng Zhang