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#34525477   2021/09/15 To Up

Effect of human periodontal ligament stem cell-derived extracellular vesicles on macrophage pyroptosis and periodontal inflammatory injury in periodontitis.


Xiao-Dong Han, Hong-Min Chen, Cai Li

2296 related Products with: Effect of human periodontal ligament stem cell-derived extracellular vesicles on macrophage pyroptosis and periodontal inflammatory injury in periodontitis.

2ug10 ug5ug2ug5ug2ug5ug1 mg2ug100ug Lyophilized5ug96 wells (1 kit)

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#34525476   2021/09/15 To Up

Expression and clinical significance of lncRNA OSER1-AS1 in peripheral blood of patients with non-small cell lung cancer.


Peirui Chen, Zhen Qin, Xiaokang Sun, Junrong Yang, Jing Lv, Mingqiang Diao

1489 related Products with: Expression and clinical significance of lncRNA OSER1-AS1 in peripheral blood of patients with non-small cell lung cancer.



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#34525462   2021/09/15 To Up

A highly transparent tri-polymer complexhydrogel of HA, Collagen and 4-arm-PEG as potential vitreous substitute.

There is a requirement of removal and replacement of vitreous for various ophthalmic diseases, e.g., retinopathy and retinal detachment. Clinical tamponades, e.g., silicone oil and fluorinated gases are used but limited due to their toxicity and some complications. A lot of polymer-based materials have been tested and proposed as vitreous substitute, but till date, there is no ideal vitreous substitute available. Thus, it requires to develop an improved vitreous substitute which will be highly suitable for vitreous replacement. We have developed tri-polymer complex in situ hydrogels by crosslinking among hyaluronic acid (HA), collagen (Coll) and 4-arm-polyethylene glycol (PEG). All the developed hydrogels are biocompatible with NIH 3T3 mouse fibroblast cells, having pH in the range 7-7.44 and refractive index in the range 1.333-1.345. The developed hydrogels are highly transparent, showing transmittance >97%. FTIR study shows that the hydrogel was crosslinked by amide bond formation between HA and PEG, and between collagen and PEG. The rheological study shows that all the developed hydrogels exhibit viscoelastic behavior and all the hydrogels have storage modulus values (>100 pa) which is greater than loss modulus values-indicating sufficient elasticity for vitreous application. The elastic nature of the hydrogel increases with the increase in PEG concentration. The gel is formed in between 2 to 3 min-indicating its application in situ. The viscosity of the developed hydrogels shows shear thinning behavior. The pre-gel solution of the hydrogel is injectable through a 22 G needle -indicating its application in situ through vitrectomy surgery. All the hydrogels are hydrophilic and have water content of 96% approximately. Thus, the results show the positive properties for its application as a potential vitreous substitute.
Indu Yadav, Shiv Dutt Purohit, Hemant Singh, Neeladri Singha Das, Partha Roy, Narayan Chandra Mishra

1228 related Products with: A highly transparent tri-polymer complexhydrogel of HA, Collagen and 4-arm-PEG as potential vitreous substitute.

10 plates

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#34525437   2021/09/07 To Up

Ultrasound and heat treatment effects on Staphylococcus aureus cell viability in orange juice.

Ultrasounds are being considered an excellent alternative technology in juice preservation. Yet, when combined with heat treatment, the process seems to be further intensified. This work aimed to evaluate and compare the impact of ultrasounds and heat treatments, when applied alone or in combination, on Staphylococcus aureus survival in orange juice. Inoculated commercial pasteurized orange juice was treated at different times at 20, 30, 40, 50 and 60 °C. SEM analyses were applied to identify morphological changes in S. aureus cells appearance. The microbial inactivation data were fitted using two mathematical models, depending on the behaviour observed. Sonication at 20, 30, and 40 °C induced 4.02 ± 0.52, 3.80 ± 0.49 and 4.30 ± 0.74 log cycles reduction of S. aureus after treatments of 90, 60 and 60 min, respectively. The heat treatments at the same temperatures had no impact on S. aureus survival. When 50 and 60 °C were applied, more than 5-log reductions were attained for both thermosonication and heat treatments alone. A synergistic effect was observed between sonication and high temperatures. At 50 °C, the thermosonication reduced the treatment time from 60 to 35 min and the microbial load from 5.14 ± 0.08 to 10.76 ± 0.28 log cycles reduction, compared to heat treatment alone. Results from SEM images showed that cells undergo membrane damage during sonication exposure. This was observed by collapsed cells, cell disruption, and holes in the cell's membrane. Thermosonication proved to be a viable alternative to thermal pasteurization of orange juice since milder treatments can be safely applied, improving the final product quality.
Akila Amir Tahi, Sérgio Sousa, Khodir Madani, Cristina L M Silva, Fátima A Miller

2823 related Products with: Ultrasound and heat treatment effects on Staphylococcus aureus cell viability in orange juice.

1 kit5000100 µg1x10e7 cells-100ug Lyophilized1 kit50 ml100 μg200

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#34525422   2021/09/04 To Up

Adoptive T-cell immunotherapy in digestive tract malignancies: Current challenges and future perspectives.

Multiple systemic treatments are currently available for advanced cancers of the digestive tract, but none of them is curative. Adoptive T-cell immunotherapy refers to the extraction, modification and re-infusion of autologous or allogenic T lymphocytes for therapeutic purposes. A number of clinical trials have investigated either non-engineered T cells (i.e., lymphokine-activated killer cells, cytokine induced killer cells, or tumor-infiltrating lymphocytes) or engineered T cells (T cell receptor-redirected T cells or chimeric antigen receptor T cells) in patients with digestive tract malignancies over the past two decades, with variable degrees of success. While the majority of completed trials have been primarily aimed at assessing the safety of T-cell transfer strategies, a new generation of studies is being designed to formally evaluate the antitumor potential of adoptive T-cell immunotherapy in both the metastatic and adjuvant settings. In this review, we provide an overview of completed and ongoing clinical trials of passive T-cell immunotherapy in patients with cancers of the digestive tract, focusing on present obstacles and future strategies for achieving potential success.
Barbara Mandriani, Eleonora Pelle', Gaetano Pezzicoli, Jonathan Strosberg, Daniel Abate-Daga, Attilio Guarini, Mauro Cives, Camillo Porta

2368 related Products with: Adoptive T-cell immunotherapy in digestive tract malignancies: Current challenges and future perspectives.

100ug Lyophilized2 ml1 mg3 inhibitors25 ml.96 tests100 µg1x10e7 cells

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#34525396   2021/09/12 To Up

The mitochondria-targeting antioxidant MitoQ alleviated Lipopolysaccharide/ D-galactosamine-induced acute liver injury in mice.

The mitochondria are the primary source of reactive oxygen species (ROS) under pathological condition, but the significance of mitochondrial ROS in the development of Lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced acute liver injury remains unclear. In the present study, the level of mitochondrial ROS in LPS/D-Gal has been determined by MitoSox staining and the potential roles of mitochondrial ROS in LPS/D-Gal-induced liver injury have been investigated by using the mitochondria-targeting antioxidant MitoQ. The results indicated that LPS/D-Gal exposure induced the generation of mitochondrial ROS while treatment with MitoQ reduced the level of mitochondrial ROS. Treatment with MitoQ ameliorated LPS/D-Gal-induced histopathologic abnormalities, suppressed the elevation of AST and ALT, increased the survival rate of the experimental animals. Treatment with MitoQ also suppressed LPS/D-Gal-induced production of tumor necrosis factor α (TNF-α), inhibited the activities of caspase-3, caspase-8 and caspase-9, decreased the level of cleaved caspase-3 and reduced the counts of TUNEL positive cells. These results indicate that mitochondrial ROS is involved in the development of LPS-induced acute liver injury and the mitochondria-targeting antioxidant MitoQ might have potential value for the treatment of inflammation-based acute liver injury.
Kai Hu, Lidan Xiao, Longjiang Li, Yi Shen, Yongqiang Yang, Jiayi Huang, Yaping Wang, Li Zhang, Sha Wen, Li Tang

1117 related Products with: The mitochondria-targeting antioxidant MitoQ alleviated Lipopolysaccharide/ D-galactosamine-induced acute liver injury in mice.

100 ul400 ug96 wells

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#34525386   // To Up

Derivation of feeder-free human extended pluripotent stem cells.


Ran Zheng, Ting Geng, Dan-Ya Wu, Tianzhe Zhang, Hai-Nan He, Hai-Ning Du, Donghui Zhang, Yi-Liang Miao, Wei Jiang

2655 related Products with: Derivation of feeder-free human extended pluripotent stem cells.

1 mg110 ug1.00 flask1 mg1 mg100 1 kit(96 Wells)1.00 flask1.00 flask1 mgOne 96-Well Strip Micropl

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#34525385   // To Up

High-throughput screening on cochlear organoids identifies VEGFR-MEK-TGFB1 signaling promoting hair cell reprogramming.

Hair cell degeneration is a major cause of sensorineural hearing loss. Hair cells in mammalian cochlea do not spontaneously regenerate, posing a great challenge for restoration of hearing. Here, we establish a robust, high-throughput cochlear organoid platform that facilitates 3D expansion of cochlear progenitor cells and differentiation of hair cells in a temporally regulated manner. High-throughput screening of the FDA-approved drug library identified regorafenib, a VEGFR inhibitor, as a potent small molecule for hair cell differentiation. Regorafenib also promotes reprogramming and maturation of hair cells in both normal and neomycin-damaged cochlear explants. Mechanistically, inhibition of VEGFR suppresses TGFB1 expression via the MEK pathway and TGFB1 downregulation directly mediates the effect of regorafenib on hair cell reprogramming. Our study not only demonstrates the power of a cochlear organoid platform in high-throughput analyses of hair cell physiology but also highlights VEGFR-MEK-TGFB1 signaling crosstalk as a potential target for hair cell regeneration and hearing restoration.
Qing Liu, Linqing Zhang, Min-Sheng Zhu, Guoqiang Wan

1594 related Products with: High-throughput screening on cochlear organoids identifies VEGFR-MEK-TGFB1 signaling promoting hair cell reprogramming.

1.5 x 10^6 cells2 Pieces/Box400Tests2 Sample Kit4 Sample Kit4 Sample Kit2 Sample Kit1.5x10(6) cells

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