Search results for: Chicken
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#33071350 2020/07/18 To Up
Detecting mislabelling in meat products using PCR-FINS.Economically motivated adulteration (EMA) or misrepresentation of meat products is of concern, especially in developing countries, due to obvious health hazards and religious sensitivities. As Indian cooking involves prolonged heat treatments and addition of spices and condiments, species authentication of food, especially meat products, may be challenging. This study evaluated the efficacy of Polymerase Chain Reaction-Forensically Informative Sequencing (PCR-FINS) in meat speciation of highly processed meat. Further the prevalence of mislabelling in processed and deeply cooked meat products being sold in supermarkets and restaurants in a south Indian city was investigated. FINS targeting the mitochondrial gene and the ATP synthase gene was applied to identify meat species of 106 meat products labelled as chicken, beef, carabeef, mutton and pork. Mislabelling was detected in more than half of mutton (52.3%) and carabeef (55.5%), and in under a third (27.2%) of beef products. PCR-FINS is a reliable method for meat species identification even in highly processed food but there is a need for appropriate universal primers which can target all common species used in meat products. This study is the first of its kind from the South Indian state of Kerala.
Manju Soman, Robin J Paul, Mini Antony, Soumya Padinjarattath Sasidharan 50 UG100 μg100ug Lyophilized100 μg100ug 1 G100ug Lyophilized
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#33069524 2020/10/10 To Up
Effects of pulsed electric fields on the conformation and gelation properties of myofibrillar proteins isolated from pale, soft, exudative (PSE)-like chicken breast meat: A molecular dynamics study.The potential of pulsed electric field (PEF) of different intensities (8, 18, and 28 kV/cm) on the conformation and gelation properties of myofibrillar proteins (MPs) extracted from pale, soft, and exudative-like (PSE-like) chicken meat was investigated. The results showed a positive correlation between gelation properties and PEF intensities in the range of 8-18 kV/cm; however, a further increase in intensity had a negative impact. Optimized PEF treatment (18 kV/cm) was capable of inducing MPs with a relatively small particle size, thus contributing to the production of a more homogeneous gel structure. The water distribution and mobility in the gel system significantly changed with increasing PEF intensities, the proportion of immobilized water (P) increased, and that of free water (P) decreased. Based on molecular dynamics simulations (MDS), an increasing trend in the number of hydrogen bonds and a reduction in the radius of gyration (Rg) after PEF treatment.
Ming Dong, Huixin Tian, Yujuan Xu, Minyi Han, Xinglian Xu
2406 related Products with: Effects of pulsed electric fields on the conformation and gelation properties of myofibrillar proteins isolated from pale, soft, exudative (PSE)-like chicken breast meat: A molecular dynamics study.2mg100ul100 1mg1mg50100mg1001010mg20100ug Lyophilized
#33069243 2020/10/17 To Up
Analysis of the microRNA expression profiles of chicken dendritic cells in response to H9N2 avian influenza virus infection.MicroRNA (miRNA) plays a key role in virus-host interactions. Here, we employed deep sequencing technology to determine cellular miRNA expression profiles in chicken dendritic cells infected with H9N2 avian influenza virus (AIV). A total of 66 known and 36 novel miRNAs were differently expressed upon H9N2 infection, including 72 up-regulated and 30 down-regulated miRNAs. Functional analysis showed that the predicted targets of these miRNAs were significantly enriched in several pathways including endocytosis, notch, lysosome, p53, RIG-I-like and NOD-like receptor signaling pathways. These data provide valuable information for further investigating the roles of miRNA in AIV pathogenesis and host defense response.
Jing Yang, Xinmei Huang, Yuzhuo Liu, Dongmin Zhao, Kaikai Han, Lijiao Zhang, Yin Li, Qingtao Liu
1436 related Products with: Analysis of the microRNA expression profiles of chicken dendritic cells in response to H9N2 avian influenza virus infection.251002525252 ml0.1 mg50 1 mL1 mg10
#33069231 2020/10/17 To Up
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST.Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates. Upon serotyping of the isolates, the antimicrobial susceptibility profiling using a panel of 9 commonly used antimicrobials was done. Subsequently, the molecular profiles of all the isolates were further determined using Pulsed Field Gel Electrophoresis (PFGE) and the Whole Genome Multi-Locus Sequence Type (wgMLST) analysis in order to obtain the sequence types.
Zunita Zakaria, Latiffah Hassan, Zawiyah Sharif, Norazah Ahmad, Rohaya Mohd Ali, Suraya Amir Husin, Nor Hazrin Binti Abd Hazis, Nor Fitriah Mohamed Sohaimi, Shafini Abu Bakar, Bashiru Garba
2833 related Products with: Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST.200ug1 mg100ug Lyophilized1,000 tests100ug100ug100 mg100ug Lyophilized1000 tests
#33065877 // To Up
Marek's disease virus and skin interactions.Marek's disease virus (MDV) is a highly contagious herpesvirus which induces immunosuppression and T-cell lymphoma in chicken. This virus still circulates in flocks despite forty years of vaccination, with important economical losses at the world level. The feather follicles, which allow feathers morphogenesis and their anchor into the skin, are the unique known source of MDV excretion. This tissue causes environment contamination and MDV bird-to-bird transmission. Epithelial cells from the feather follicles are the only identified cells, in which high levels of infectious mature virions are visible by transmission electron microscopy and from which cell-free infectious virions have been purified. Finally, feathers harvested on animals and poultry dust are today considered as excellent materials in order to follow vaccination, circulation of pathogenic viruses and environment contamination. This article aims at summarizing the current knowledge on MDV-skin interactions and at suggesting new approaches which could solve important questions on MDV biology.
Mathilde Couteaudier, Caroline Denesvre1 mg200 200 500100ug
#33069036 2020/10/06 To Up
Duration of protective immunity induced by Mycoplasma gallisepticum strain ts-304 vaccine in chickens.Mycoplasma gallisepticum (MG) is an important pathogen of poultry worldwide, causing chronic respiratory disease in chickens and turkeys. MG ts-304 is a GapA positive clone recovered from Vaxsafe MG (strain ts-11) that has been shown to be safe in chickens when delivered by the eye drop route to 3-week-old specific-pathogen-free chickens and to confer protection against challenge at 4 weeks after vaccination, as measured by tracheal mucosal thickness and air sac lesion scores. In this study, specific pathogen-free chickens (SPF) were vaccinated with a single dose of the MG ts-304 vaccine (10 colour changing units) at 3 weeks of age and experimentally challenged by aerosol with the virulent M. gallisepticum strain Ap3AS at 40, 48 and 57 weeks after vaccination. There were no significant differences in tracheal mucosal thickness 2 weeks after challenge between chickens challenged at the three time points, or between the vaccinated birds after challenge and unvaccinated/unchallenged control birds. Thus there was clear evidence that the immunity conferred by vaccination with the MG ts-304 vaccine resulted in significant protection against tracheitis in chickens that extended to, but was highly likely to exceed, 57 weeks after vaccination and that similar long term protective immunity could be expected to be conferred by a vaccine dose lower than that used in this study.
Anna Kanci Condello, Sathya N Kulappu Arachchige, Pollob K Shil, Gregory J Underwood, Amir H Noormohammadi, Philip F Markham, Nadeeka K Wawegama, Glenn F Browning
2891 related Products with: Duration of protective immunity induced by Mycoplasma gallisepticum strain ts-304 vaccine in chickens.100ul96T1 mg2ug100 ul5ug96 assays100 ul400 ug50 ul100ug400 ug
#33068825 2020/09/19 To Up
Antimicrobial susceptibility of pathogenic mycoplasmas in chickens in Asia.Mycoplasma synoviae (n = 26) and M. gallisepticum (n = 11) isolates were gained from 164 clinical samples collected from China, India, Indonesia, Malaysia, Philippines, Republic of Korea and Thailand. Most isolates were from commercial chicken production systems. A method of filtering (0.45 μm) samples immediately after collection was convenient allowing over a week for transit to the laboratory. Minimum inhibitory concentrations (MICs) were characterized by a broth microdilution method to enrofloxacin, difloxacin, oxytetracycline, chlortetracycline, doxycycline, tylosin, tilmicosin, tylvalosin, tiamulin, florfenicol, lincomycin, spectinomycin and lincomycin and spectinomycin combination (1:2). Increased MICs to various antimicrobials were seen in different isolates but appeared largely unrelated to the antimicrobial treatment histories. Overall, the results were similar to other MIC surveys around the world. Generally, low MICs to tetracyclines, tiamulin and tylvalosin were observed. Increased tilmicosin MICs were observed in both M. synoviae and M. gallisepticum isolates (≥64 μg/ml MIC values) and this was seen in all isolates with high tylosin MICs. Increases in lincomycin MICs were mostly associated with increases in tilmicosin MICs. The results also suggested that antimicrobial use after mycoplasma vaccination may interfere with vaccine strain persistence and efficacy (field strains were more commonly observed in flocks that had treatments after vaccination) and this area warrants more investigation. The study shows that isolation and MIC determination can be done from remote locations and suggests that this may provide information that will allow more effective use of antimicrobials or other methods of control of avian mycoplasma in chickens (e.g. live vaccines) and therefore more responsible use of antimicrobials from a one health perspective.
Chris J Morrow, Zsuzsa Kreizinger, Robin R Achari, Katinka Bekő, Cécile Yvon, Miklós Gyuranecz
1263 related Products with: Antimicrobial susceptibility of pathogenic mycoplasmas in chickens in Asia.100 μg100ug Lyophilized100 μg96 samples100ug Lyophilized1 g100ug Lyophilized 25 G
#33068733 2020/10/14 To Up
Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus.Infectious bronchitis (IB) is an economically important disease of poultry that also serve as model for the understanding of other coronaviruses associated diseases. IB is considered as a major challenge to the poultry industry worldwide as a result of its effect on egg production, weight gain as well as mortality. Different IBV genotypes continue to emerge, thus, the need for broad based vaccines to curb the disease. Based on bioinformatic data obtained in this study, sets of monovalent (either M41 or CR88) and bivalent DNA vaccines encoding the S1 glycoprotein from two different strains namely, M41 and CR88 were developed. The candidate vaccine was further encapsulated with a chitosan-saponin nanoparticle with the view to enhance its immunogenicity. Following in vitro characterization of the constructs, the vaccine candidates were tested in specific pathogen free (SPF) chickens. Analysis of humoral responses revealed a significant increase in anti-IBV antibody after immunization with the bivalent DNA plasmid (pBudCR88-S1/M41-S1). Likewise, cell mediated immune (CMI) response was significantly higher in vaccinated groups as compared to the unvaccinated chickens. Vaccinated chickens exhibited milder clinical signs as well as tracheal and kidney lesion scores following virus challenge as compared to the control groups. Additionally, encapsulation of the bivalent DNA vaccine with chitosan-saponin nanoparticles was found to improve protection against challenge with IBV strains M41 and CR88 as revealed by a significant reduction (p < 0.05) in oropharyngeal and cloacal virus sheddings following challenges with each of the two viruses. In contrast, monovalent IB-DNA vaccines were protective against homologous virus challenge. Moreover, nanoencasulation of the candidate vaccine was demostrated to abrogate the need for booster vaccination. In conclusion, this study demonstrates the immunogenic potentials of a bivalent IB-DNA vaccine and the use of chitosan-saponin nanoparticle to enhance its response. This development could serve as alternative strategy for the control of IB in poultry.
Faruku Bande, Siti Suri Arshad, Mohd Hair Bejo, Abdul Rahman Omar, Hassan Moeini, Saed Khadkodae, Tan Sheau Wei, Yeap Swee Keong, Yusuf Abba, Ibrahim Abubakar Anka
1209 related Products with: Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus.100ug100ug Lyophilized 25 MG500 tests100ul500gm 100ul96T25 mg100 ug/vial100ug Lyophilized20
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