Search results for: Coating
#34525168 2021/09/15 To Up
Depletion forces drive reversible capture of live bacteria on non-adhesive surfaces.Because bacterial adhesion to surfaces is associated with infections and biofilm growth, it has been a longstanding goal to develop coatings that minimize biomolecular adsorption and eliminate bacteria adhesion. We demonstrate that, even on carefully-engineered non-bioadhesive coatings such as polyethylene glycol (PEG) layers that prevent biomolecule adsorption and cell adhesion, depletion interactions from non-adsorbing polymer in solution (such as 10 K PEG or 100 K PEO) can cause adhesion and retention of cells, defeating the antifouling functionality of the coating. The cells are immobilized and remain viable on the timescale of the study, at least up to 45 minutes. When the polymer solution is replaced by buffer, cells rapidly escape from the surface, consistent with expectations for the reversibility of depletion attractions. The dissolved polymer additionally causes cells to aggregate in solution and aggregates rapidly dissociate to singlets upon tenfold dilution in buffer, also consistent with depletion. Hydrodynamic forces can substantially reduce the adhesion of aggregates on surfaces in conditions where single cells adhere depletion. The findings reported here suggest that because bacteria thrive in polymer-rich environments both and , depletion interactions may make it impossible to avoid bacterial retention on surfaces.
Wuqi Amy Niu, Sylvia L Rivera, M Sloan Siegrist, Maria M Santore
2669 related Products with: Depletion forces drive reversible capture of live bacteria on non-adhesive surfaces.1 KIT150 mg96 T 25 ml Ready-to-use 1 kit(s) 100 stainings
#34525143 2021/09/15 To Up
Understanding the close encounter of heme proteins with carboxylated multiwalled carbon nanotubes: a case study of contradictory stability trend for hemoglobin and myoglobin.Carbon nanotubes (CNTs) are one of the unique and promising nanomaterials that possess plenty of applications, such as biosensors, advanced drug delivery systems and biotechnology. CNTs bind rapidly with proteins, which result in the formation of a protein coating layer known as a "protein corona" around the surface of the nanomaterial. This hinders their applications as a drug carrier and influences the properties of biological macromolecules. The present work focuses on studying the thermal stability and molecular level interactions of two heme proteins, hemoglobin (Hb) and myoglobin (Mb), in the presence of carboxylated functionalized multi-walled CNTs (CA-MWCNTs). Through the current study, the following steps have been taken to distinguish the biocompatibility of the hydrophilic surface CA-MWCNTs for heme proteins a series of spectroscopic techniques and differential scanning calorimetry (DSC). UV-Visible and steady-state fluorescence spectroscopy were used to reveal changes in the aromatic amino acid residues of heme proteins upon the addition of CA-MWCNTs. Circular dichroism spectroscopy (CD) shows the alteration in the native structure of proteins in the presence of the nanomaterial. A tremendous increase in the size of the protein CA-MWCNTs system is observed in dynamic light scattering (DLS), which clearly manifests the protein corona formation. Unexpectedly, both proteins interact differently with CA-MWCNTs, which is observed in CD spectroscopy and DSC. In the presence of CA-MWCNTs, an increase in the transition temperature () was observed for Hb, while the value decreases for Mb. Different interactions with proteins at the molecular scale may be the reason for this unexpected behavior. Henceforth, the present results can help in the design of the next-generation drug carrier nanomaterials with the idea of the heme protein corona formation prior to development.
Sumit Kumar, Krishan Kumar, Ritu Yadav, Prasanna Kukutla, Nagaraju Devunuri, Nirmala Deenadayalu, Pannuru Venkatesu
1903 related Products with: Understanding the close encounter of heme proteins with carboxylated multiwalled carbon nanotubes: a case study of contradictory stability trend for hemoglobin and myoglobin.100ul
#34525052 // To Up
Visible to near-infrared nearly perfect absorption from alternate silica and chromium layers deposited by magnetron sputtering.We present a novel, to the best of our knowledge, broadband and angle-insensitive nearly perfect absorber design composed of alternate silica and chromium layers. We show that by depositing a chromium nanofilm on a chromium substrate with a silica spacer, the absorption will significantly enhance from the visible to near-infrared. Then, another silica film is placed on the top of the layered structure as an antireflection coating, resulting in the broadband near-perfect absorption. We fabricate the proposed absorber by magnetron sputtering. The measured results show that our device has an average absorption over 97% in a wide range of wavelengths ranging from 350 to 1170 nm, and its absorption performance exhibits a good angular tolerance up to 50Â°. The presented absorber design offers a good prospect for large-scale and low-cost manufacturing of absorption-based optoelectronic devices.
Jiaxin Wang, Jiannan Dong, Yige Cheng, Zhenlong Xie, Yihang Chen
1600 related Products with: Visible to near-infrared nearly perfect absorption from alternate silica and chromium layers deposited by magnetron sputtering.1 module96 wells (1 kit)96/kit 100 G1 kit(96 Wells)1 ml100 6 ml Ready-to-use 1 kit1 module200ug
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#34524812 2021/09/15 To Up
Ionic Hydrogels Based Wearable Sensors to Monitor the Solar Radiation Dose for Vitamin D Production and Sunburn Prevention.Wearable solar radiation sensors based on ionic hydrogels are facilely prepared to simultaneously monitor the radiation dose for the production of vitamin D and the prevention of sunburn. Tetramethylethylenediamine (TEMED) is neutralized with acrylic acid (AA) to obtain tetramethylethylenediamine acrylate (TEMEDA), which is further polymerized with acrylamide by a free radical reaction. By simply adding MB or NR during the polymerization, the final obtained ionic hydrogels can indicate solar radiation. Due to the extent of discoloration, the discoloration speed of MB and NR is correlated to the radiation dose. This wearable sensor can indicate the solar radiation dose required by the human body to synthesize vitamin D through the discoloration of the ionized hydrogel of MB, whereas those with NR are able to illustrate the threshold of radiation dose that causes potential skin hurt. Therefore, the benefit and drawback of solar radiation can be well balanced by optimizing the exposure time to solar irradiation. In addition, polyurethane cross-linked with a thermoresponsive coating is used as band for this wearable sensor. Due to the hydrophilicity below its transition temperature, the cross-linked band possesses the easy cleaning capability of stains after the daily wear. Such type of wearable sensor can be broadly used for monitoring the solar radiation, especially in outdoor activities.
Li Lin, Liangen Shen, Junfeng Zhang, Yiyan Xu, Zheng Fang, Peter MÃ¼ller-Buschbaum, Qi Zhong
2260 related Products with: Ionic Hydrogels Based Wearable Sensors to Monitor the Solar Radiation Dose for Vitamin D Production and Sunburn Prevention.10 mg 5 G200 ug0.1 mg100 IU100Tests
#34524805 2021/09/15 To Up
Nondestructive Microanalysis of Thin-Film Coatings on Historic Metal Threads.A new standards-based scanning electron microscopy with the energy-dispersive X-ray spectrometry (SEM-EDS) quantification method was used to analyze the thin-film coating of an 18th century French textile decorated with metal threads in variable pressure conditions. This analytical technique can allow for nondestructive quantitative characterization of the near surface of cultural heritage objects small enough to be placed in an SEM chamber that may contain corrosion products, without applying a conductive coating. A multivoltage analysis consisting of measurements taken at a series of electron beam energies was obtained and input into a film thickness and composition (FTC) computational model to characterize a layered Au on Ag reference material, in addition to a historic metal thread. Using the FTC computation, the thread coating was determined to be an alloy â 80% Au 20% Ag on a nominally pure Ag substrate, and this composition matches a minimum gold standard allowed for goods around the time of manufacture. The computed gilding thicknesses range from single digit nm to 300 nm depending upon surface inhomogeneities formed during the production of the thread. Interaction volumes and X-ray spectra generated by Monte Carlo modeling are consistent with the measured gilding thicknesses and compositions. Validation of the FTC-computed gilding composition and thickness variations were obtained by cross-sectional analysis.
Aleksandra Popowich, Thomas Lam, Edward P Vicenzi
1559 related Products with: Nondestructive Microanalysis of Thin-Film Coatings on Historic Metal Threads.20 ul100ug100μg5100μg0,0550 ul20 ul8100μg0,4 6 ml
#34523902 2021/09/15 To Up
Machine Learning Prediction of TiO-Coating Wettability Tuned via UV Exposure.Surfaces with extreme wettability (too low, superhydrophobic; too high, superhydrophilic) have attracted considerable attention over the past two decades. Titanium dioxide (TiO) has been one of the most popular components for generating superhydrophobic/hydrophilic coatings. Combining TiO with ethanol and a commercial fluoroacrylic copolymer dispersion, known as PMC, can produce coatings with water contact angles approaching 170Â°. Another property of interest for this specific TiO formulation is its photocatalytic behavior, which causes the contact angle of water to be gradually reduced with rising timed exposure to UV light. While this formulation has been employed in many studies, there exists no quantitative guidance to determine or tune the contact angle (and thus wettability) with the composition of the coating and UV exposure time. In this article, machine learning models are employed to predict the required UV exposure time for any specified TiO/PMC coating composition to attain a certain wettability (UV-reduced contact angle). For that purpose, eight different coating compositions were applied to glass slides and exposed to UV light for different time intervals. The collected contact-angle data was supplied to different regression models to designate the best method to predict the required UV exposure time for a prespecified wettability. Two types of machine learning models were used: (1) parametric and (2) nonparametric. The results showed a nonlinear behavior between the coating formulation and its contact angle attained after timed UV exposure. Nonparametric methods showed high accuracy and stability with general regression neural network (GRNN) performing best with an accuracy of 0.971, 0.977, and 0.933 on the test, train, and unseen data set, respectively. The present study not only provides quantitative guidance for producing coatings of specified wettability, but also presents a generalized methodology that could be employed for other functional coatings in technological applications requiring precise fluid/surface interactions.
Mohamad Jafari Gukeh, Shashwata Moitra, Ali Noaman Ibrahim, Sybil Derrible, Constantine M Megaridis
1299 related Products with: Machine Learning Prediction of TiO-Coating Wettability Tuned via UV Exposure.75мg/vial1 1 Liter (5x Univ 50μg/vial100ug/vial100ug Lyophilized20μg/vial100 ug/vial240.00 ml10001000pcs100ug/vial
#34523626 2021/09/15 To Up
A polydopamine-gated biodegradable cascade nanoreactor for pH-triggered and photothermal-enhanced tumor-specific nanocatalytic therapy.Despite the great potential of cascade catalytic reactions in tumor treatment, uncontrolled catalytic activities lead to inevitable off-target toxicity to normal tissues, which greatly hampers their clinical conversion. Herein, an intelligent cascade nanoreactor ([email protected], hMAP) was constructed by depositing glucose oxidase (GOx)-mimicking ultrasmall gold nanoparticles (Au NPs) into honeycomb-shaped manganese oxide (hMnO) nanostructures and then coating them with polydopamine (PDA) to achieve pH-responsive and photothermal-enhanced nanocatalytic therapy. Upon exposure to the mild acidic tumor microenvironment (TME), the PDA gatekeeper would collapse, and the inner hMnO could simultaneously deplete glutathione (GSH) and generate Mn, while a considerable amount of HO produced from the oxidation of glucose by GOx-mimicking Au NPs could accelerate the Mn-mediated Fenton-like reaction, yielding sufficient highly toxic ËOH. More importantly, the pH-responsive cascade reaction between Au NPs and hMnO could be further enhanced by localized hyperthermia induced from PDA under near-infrared (NIR) laser irradiation, thereby inducing significant cell apoptosis and tumor inhibition . This work provided a promising paradigm by innovatively designing a TME-responsive and photothermal-enhanced cascade catalytic nanoreactor for safe and efficient cancer therapy.
Fanghui Chen, Peijing An, Ling Liu, Zhiguo Gao, Yaojia Li, Yuchen Zhang, Baiwang Sun, Jiancheng Zhou
2579 related Products with: A polydopamine-gated biodegradable cascade nanoreactor for pH-triggered and photothermal-enhanced tumor-specific nanocatalytic therapy.0.1 mg0.2 mg1 LITRE100 ml25 µg1 ml0.1 ml
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#34523343 2021/09/15 To Up
Imaging Sensor for the Detection of the Flow Battery Via Weak Value Amplification.Flow battery electrodes are vital for performing redox reactions, and an in-depth understanding of reaction kinetics and spatial distribution differences in electrodes is very important for improving the efficiency of electrochemical reactions. In this study, a reflection-type phase-sensitive weak measurement imaging system was developed for the detection of flow batteries. The phase difference between two polarization components in total internal reflection caused by electrode redox processes was measured by weak value amplification. The resulting refractive index resolution of the imaging system was estimated to be 2.8-4.2 Ã 10 RIU. The real-time monitoring ability of the system was demonstrated by linear sweep voltammetry tests of vanadium redox batteries. Compared to traditional optical methods, the proposed weak measurement imaging sensor did not require coating, as it can be used in acid electrolytes of vanadium flow batteries. Meanwhile, the weak value amplification effect led to a higher resolution than the total internal reflection system shown in our previous work, thereby resulting in more accurate detection of electrochemical reactions. In sum, the proposed sensor looks very promising for the detection of electrochemical reactions in flow batteries, water splitting, electrochemical corrosion, and electrocatalysis.
Yang Xu, Chongqi Zhou, Lixuan Shi, Xiaonan Zhang, Tian Guan, Cuixia Guo, Zhangyan Li, Xinhui Xing, Yanhong Ji, Le Liu, Yonghong He
1128 related Products with: Imaging Sensor for the Detection of the Flow Battery Via Weak Value Amplification.2000 IU100.00 ul500 Units110 100 G100 IU2x96 well plate0.1 mg
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