Search results for: Conjugated
#33657498 2021/02/26 To Up
Lactobionic acid-modified thymine-chitosan nanoparticles as potential carriers for methotrexate delivery.In order to achieve efficient delivery of methotrexate (MTX), thymine-chitosan nanoparticles (Thy-Cs NPs) were prepared, and further decorated with lactobionic acid (LA) to obtain tumor-targeting nanoparticles (LA-Thy-Cs NPs). These nanoparticles possessed a regular spherical structure with the average size about 190-250 nm and narrow size distribution, which were kinetically stable in the physiological environment. Due to electrostatic interactions and multiple hydrogen-bonding interactions between MTX and carriers, MTX was loaded into Thy-Cs NPs with high drug loading content (~20%). MTX release from Thy-Cs NPs was significantly accelerated in the mildly acidic environment due to the destruction of two types of non-covalent interactions. In vitro cell experiments demonstrated that LA-Thy-Cs NPs could be efficiently internalized into hepatoma carcinoma cells, leading to higher cytotoxicity. Moreover, MTX-loaded LA-Thy-Cs NPs performed an enhanced growth inhibition in three-dimensional multicellular tumor spheroids. Thus, the LA decorated thymine-chitosan nanocarriers can be a promising candidate for efficient delivery of MTX.
Jun Wang, Zongyong Zhang, Yilong Ai, Fang Liu, Min-Min Chen, Dahai Liu
2784 related Products with: Lactobionic acid-modified thymine-chitosan nanoparticles as potential carriers for methotrexate delivery.250 assays100ug Lyophilized 1 G100 assays100tests 1 G100ug Lyophilized 1KG100 assays 100 G100ug Lyophilized
#33657199 2021/03/03 To Up
An anthracene extended viologen-incorporated ionic porous organic polymer for efficient aerobic photocatalysis and antibacterial activity.A new conjugated ionic porous organic polymer (AN-POP), incorporated with anthracene-extended viologen, has been rationally designed and prepared to explore its dual functions in photocatalytic oxidation and bacterial killing. Compared with its anthracene-free counterpart (BD-POP), AN-POP showed a superior photocatalytic oxidation performance and antibacterial activity demonstrating the critical role of an anthracene-extended viologen structure.
Lu Liu, Wei-Dong Qu, Kai-Xun Dong, Ye Qi, Wei-Tao Gong, Gui-Ling Ning, Jing-Nan Cui
1976 related Products with: An anthracene extended viologen-incorporated ionic porous organic polymer for efficient aerobic photocatalysis and antibacterial activity.100ug100ug Lyophilized25 mg5 mg 100ul100ug Lyophilized10 mg0.1 ml 8 ml 100ug Lyophilized500 MG100ug
#33656323 2021/03/03 To Up
Long-Acting Human Interleukin 2 Bioconjugate Modified with Fatty Acids by Sortase A.Human Interleukin 2 (IL-2) has already achieved impressive results as a therapeutic agent for cancer and autoimmune diseases. However, one of the limitations associated with the clinical application of IL-2 is its short half-life owing to rapid clearance by the kidneys. Modification with fatty acids, as an albumin noncovalent ligand with the advantage of deep penetration into tissues and high activity-to-mass ratio, is a commonly used approach to improve the half-life of native peptides and proteins. In this investigation, we attempted to extend the half-life of IL-2 through conjugation with a fatty acid using sortase A (srtA). We initially designed and optimized three IL-2 analogues with different peptide linkers between the C-terminus of IL-2 and srtA recognition sequence (LPETG). Among these, analogue A3 was validated as the optimal IL-2 analogue for further modification. Next, six fatty acid moieties with the same fatty acid and different hydrophilic spacers were conjugated to A3 through srtA. The six bioconjugates generated were screened for biological activity, among which bioconjugate B6 was identified as near-optimal to IL-2. Additionally, B6 could effectively bind albumin through the conjugated fatty acid, which contributed to a significant improvement in its pharmacokinetic properties . In summary, we have developed a novel IL-2 bioconjugate, B6, modified with fatty acids using srtA, which may effectively serve as a new-generation long-acting IL-2 immunotherapeutic agent.
Mengxin Qian, Qingbin Zhang, Jianguang Lu, Jinhua Zhang, Yapeng Wang, Wenwen Shangguan, Meiqing Feng, Jun Feng
2228 related Products with: Long-Acting Human Interleukin 2 Bioconjugate Modified with Fatty Acids by Sortase A.5ug1 kit(96 Wells)96T100 μg100ug Lyophilized 100ul50 ug10.00 ug100ug Lyophilized20μg/vial100ug Lyophilized
#33656311 2021/03/03 To Up
Paintable Hybrids with Thermally Stable Dual Emission Composed of Tetraphenylethene-Integrated POSS and MEH-PPV for Heat-Resistant White-Light Luminophores.Thermally stable dual emission followed by white-light luminescence from hybrid materials is reported. Hybrid films were prepared with a spin-coating method with the mixture solution containing tetraphenylethene (TPE)-integrated polyhedral oligomeric silsesquioxane (POSS) and poly[2-methoxy-5-(2'-ethylhexyloxy)-1,4-phenylene vinylene] (). TPE-tethered POSS () showed high compatibility with . Therefore, homogeneous films with variable concentrations of were obtained. Owing to good dispersion of rigid silica cubes into matrices, POSS-containing films demonstrated high thermal stability toward molecular rearrangement by annealing as well as pyrolysis, similar to conventional polymer hybrids. Furthermore, it was found that was able to enhance emission efficiencies, probably by suppressing chain aggregation. By modulating introduction ratios of , dual-emission properties followed by white-light luminescence composed of cyan and orange emissions from and , respectively, were accomplished. It should be noted that these color balances can be preserved even in the high-temperature region (425 K). Finally, white-light luminescent materials with thermal durability were obtained.
Masayuki Gon, Satoru Saotome, Kazuo Tanaka, Yoshiki Chujo
2003 related Products with: Paintable Hybrids with Thermally Stable Dual Emission Composed of Tetraphenylethene-Integrated POSS and MEH-PPV for Heat-Resistant White-Light Luminophores.100 mg96T500 mg 500 ml
#33655650 2021/03/02 To Up
Randomized trial comparing radial hemostasis techniques; catechol conjugated chitosan pad (InnoSEAL) versus pneumatic compression band.Primary objectives: to compare radial artery occlusion rate (RAO) after cardiac catheterization between catecholamine-chitosan pad (InnoSEAL) and pneumatic compression device (PCD) and to compare difference in hemostasis time and radial monitoring termination time between two arms. Secondary objectives: to compare radial site bleeding and ease of use of two methods by cath-lab technicians.
Asad Z Pathan, Saba Aijaz, Sana Sheikh, Saadia Sattar
1284 related Products with: Randomized trial comparing radial hemostasis techniques; catechol conjugated chitosan pad (InnoSEAL) versus pneumatic compression band.100ug Lyophilized100ug Lyophilized100ug100ug100ug100ug Lyophilized100ug100ug100ug Lyophilized100ug100ug100ug Lyophilized
#33655441 2021/03/02 To Up
A chitosan-based nanosystem as pneumococcal vaccine delivery platform.Chitosan-based nanosystems have been described as interesting tools for antigen delivery and for enhancing the immunogenicity of nasally administered vaccines. As a possible vaccine delivery method, the chemical conjugation of chitosan nanocapsules with the Streptococcus pneumoniae cell membrane protein PsaA (pneumococcal surface adhesin A) is suggested here. The antigen PsaA, common to all pneumococcus serotypes, is expected to improve its uptake by immune cells and to activate specific T cells, generating an adaptive immune response against pneumococcus. With this aim, chitosan nanocapsules with thiol-maleimide conjugation between the polymer (chitosan) and the antigen (PsaA) were designed to enable the surface presentation of PsaA for immune cell recognition. Spherical-shaped particles, with a size of 266 ± 32 nm, positive charge of +30 ± 1 mV, and good stability profiles in simulated nasal fluids (up to 24 h) were achieved. PsaA association rates were three times higher compared with nanocapsules without covalent polymer-protein conjugation. Cytotoxicity studies in cell culture media showed non-toxic effect under 150 µg/mL concentration of nanocapsules, and subsequent studies on the maturation of immature dendritic cells in the presence of antigen-conjugated nanocapsules displayed peripheral blood mononuclear cell activation and lymphocyte differentiation after their presentation by dendritic cells. Secretion of TNFα following exposure to nanocapsules and the ability of nanocapsules to activate CD4 and CD8 T lymphocytes had also been studied. Antigen loaded nanocarrier uptake and presentation by professional presenting cells.
Sandra Robla, Maruthi Prasanna, Rubén Varela-Calviño, Cyrille Grandjean, Noemi Csaba500 tests100tests100 tests100 assays1 kit500200 assays100 assays100ug Lyophilized100 mg
#33655404 2021/03/03 To Up
Recent Advances in Clusteroluminescence.Clusteroluminescence is a phenomenon whereby the aggregation or clustering of non-conjugated electron-rich units leads to the emission of light at long wavelengths. This phenomenon was first discovered in poly(amido amine) (PAMAM) dendrimers. In recent years, clusteroluminescence has attracted growing research interest and its photophysical properties and mechanism have been thoroughly studied. In this review, we first briefly introduce the development of different types of clusteroluminogens. Then we highlight recent developments in clusteroluminescence, including mechanistic studies, the disclosure of room-temperature phosphorescence, and the extension of emission to the longer-wavelength region. Lastly, we demonstrate a few applications in various fields. With advantages such as being earth-abundant, biocompatible and biodegradable, clusteroluminogens are envisioned to be commonplace in the future.
Zhaoyu Wang, Haoke Zhang, Siqi Li, Dangyuan Lei, Ben Zhong Tang, Ruquan Ye10mg100 μg100ug100 μg0.1ml100ug100ug Lyophilized1 Set101 Set
#33654992 2019/03/20 To Up
Detection of mRNA by Whole Mount Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos.hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to chromogenic or fluorescent visualization. Although recent fluorescent methods have allowed new capabilities such as single-molecule counting, qualitative chromogenic detection remains important for many applications because of its relative simplicity, low cost and high throughput, and ease of imaging using transmitted light microscopy. A remaining challenge is combining high contrast signals with reliable genotyping after hybridization. Dextran sulfate is commonly added to the hybridization buffer to shorten development times and improve contrast, but this reagent inhibits PCR-based genotyping. This paper describes a modified protocol for hybridization in fixed whole mount zebrafish embryos using digoxigenin (DIG) labeled riboprobes that are detected with alkaline phosphatase conjugated anti-DIG antibodies and nitroblue tetrazolium (NBT)/5-bromo-4-chloro-3-indolyl-phosphate (BCIP) chromogenic substrates. To yield embryos compatible with downstream genotyping after hybridization without sacrificing contrast of the signal, this protocol omits dextran sulfate and utilizes a lower hybridization temperature.
Rachna Narayanan, Andrew C Oates
1298 related Products with: Detection of mRNA by Whole Mount Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos.5 G250 ml2x96 well plate100100ug Lyophilized 5 lt20100 tests100ug Lyophilized2x96 well plates
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#33654871 2019/09/20 To Up
Conjugation of Fab' Fragments with Fluorescent Dyes for Single-Molecule Tracking On Live Cells.Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to antibodies and wide-field microscopy methods such as total internal reflection fluorescence microscopy have become valuable tools to discern how endogenous proteins control cell biology. Yet, some technical challenges remain; in SMT, these revolve around the characteristics of the labeling reagent. A good reagent should have neutrality (in terms of not affecting the target protein's functions), tagging specificity, and a bright fluorescence signal. In addition, a long shelf-life is desirable due to the time and monetary costs associated with reagent preparation. Semiconductor-based quantum dots (Qdots) or Janelia Fluor (JF) dyes are bright and photostable, and are thus excellent candidates for SMT tagging. Neutral, high-affinity antibodies can selectively bind to target proteins. However, the bivalency of antibodies can cause simultaneous binding to two proteins, and this bridging effect can alter protein functions and behaviors. Bivalency can be avoided using monovalent Fab fragments generated by enzymatic digestion of neutral antibodies. However, conjugation of a Fab with a dye using the chemical cross-linking agent SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) requires reduction of the interchain disulfide bond within the Fab fragment, which can decrease the structural stability of the Fab and weaken its antigen-binding capability. To overcome this problem, we perform limited reduction of F(ab')2 to generate Fab' fragments using a weak reducer, cysteamine, which yields free sulfhydryl groups in the hinge region, while the interchain disulfide bond in Fab' is intact. Here, we describe a method that generates Fab' with high yield from two isoforms of IgG and conjugates the Fab' fragments with Qdots. This conjugation scheme can be applied easily to other types of dyes with similar chemical characteristics.
I-Ting Teng, Xiangning Bu, Inhee Chung
2489 related Products with: Conjugation of Fab' Fragments with Fluorescent Dyes for Single-Molecule Tracking On Live Cells.1.00 flask1.00 flask1.00 flask
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