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#34118450   2021/06/09 To Up

Combining tunable proteolytic sequences and a VEGF-mimetic peptide for the spatiotemporal control of angiogenesis within Elastin-Like Recombinamer scaffolds.

One of the main challenges in regenerative medicine is the spatiotemporal control of angiogenesis, which is key for the successful repair of many tissues, and determines the proper integration of the implant through the generation of a functional vascular network. To this end, we have designed a three-dimensional (3D) model consisting of a coaxial binary elastin-like recombinamer (ELR) tubular construct. It displays fast and slow proteolytic hydrogels on its inner and outer part, respectively, both sensitive to the urokinase plasminogen activator protease. The ELRs used to build the scaffold included crosslinkable domains to stabilize the structure and a conjugated VEGF-derived peptide (QK) to induce angiogenesis. The mechanical and morphological evaluation of the ELR hydrogels proved their suitability for soft tissue regeneration. In addition, in vitro studies evidenced the effect of the QK peptide on endothelial cell spreading and anastomosis. Moreover, immunohistochemical analyses after subcutaneous implantation of the ELR hydrogels in mice showed the induction of a low macrophage response that resolved over time. The implantation of the 3D model constructs evidenced the ability of the fast proteolytic sequence and the QK peptide to guide cell infiltration and capillary formation in the pre-designed arrangement of the constructs. These results set the basis for the application of this type of scaffolds in regenerative medicine, where spatiotemporally controlled vascularization will help in the promotion of an optimal tissue repair. STATEMENT OF SIGNIFICANCE: : Herein, we show the spatiotemporal control of angiogenesis in vivo by the combination of proteolytic sequences, with fast and slow degradation kinetics, and VEGF-mimetic peptide (QK) in a coaxial binary elastin-like recombinamer (ELR) tubular scaffold. These two bioactivities, have been previously described for angiogenesis purposes, but have never been combined. This work demonstrates that the bioactivities act synergistically in promoting cell infiltration and subsequent vascularization, thus leading to a controlled evolution in space and time of the vascular microstructure within the hydrogel-like tubular scaffold. This effect has not been showed before and holds great potential for future vascular applications, which might be of great interest for a substantial part of Acta Biomaterialia readership.
Fernando González-Pérez, Arturo Ibáñez-Fonseca, Matilde Alonso, José Carlos Rodríguez-Cabello

1024 related Products with: Combining tunable proteolytic sequences and a VEGF-mimetic peptide for the spatiotemporal control of angiogenesis within Elastin-Like Recombinamer scaffolds.

0.1 mg 96 Tests100 μg 0.1 mg 50 assays0.25 ml1100ug50 ug

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#34118331   2021/06/09 To Up

Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability.

Haloalkane dehalogenase DhaA can catalyze the hydrolytic cleavage of carbonhalogen bonds, along with production of the corresponding alcohol, a proton and a halide. However, DhaA suffers from poor environmental tolerance, such as sensitivity to high temperature, low pH and hypersaline. Arabinogalactan (AG) is a hydrophilic polysaccharide with highly branched long chains. DhaA was conjugated with AG to improve the environmental stability of DhaA in the present study. Each DhaA was averagely conjugated with 4∼5 AG molecules. Conjugation of AG essentially maintained the enzymatic activity of DhaA (91.4 %) without apparent structural alteration. The hydration layer formed by AG could reduce the solvent accessible area of DhaA and slow the protonation process, thereby improving the pH and high salt stability of DhaA. In particular, the remaining activities of the conjugate (AG-DhaA) were 35.3 % after treatment at pH4.0 for 1 h, and 80.8 % in 1 M NaCl after treatment for 16 h. As compared with DhaA, AG-DhaA showed slightly different kinetic parameters (K M of 1.90 μmol/L and k cat of 2.60 s -1).
Meiqi Wang, Weili Yu, Lijuan Shen, He Zheng, Xuan Guo, Jinyi Zhong, Tao Hu

1963 related Products with: Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability.

5 G1 module0.1 mg1 module96 wells (1 kit)5 g1 kit(96 Wells)100 50 100 1 module30 m Pcs Per Pack

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#34118044   // To Up

Norbormide-Based Probes and Their Application for Mitochondrial Imaging in Drosophila Melanogaster.

Fluorescent live imaging on Drosophila melanogaster is a microscopy technique in rapid expansion. The growing number of probes available to detect cellular components and the relatively easy genetic manipulation of fruit fly make this model one of the most used for in vivo analysis of several physiological and/or pathological processes. Here we describe the chemical synthesis of two norbormide-derived BODIPY-conjugated fluorescent probes (NRB and NRB). Moreover, we describe the larval dissection method, and subsequent live imaging acquisition. Both probes are able to label mitochondria in different Drosophila larval tissues, which allows for the characterization of mitochondrial morphological alterations by using a simple and quick method that avoids the fixation artefacts that often occur in immunofluorescence studies.
Alessia Forgiarini, Zifei Wang, Sergio Bova, Margaret Anne Brimble, Brian Hopkins, David Rennison, Genny Orso

1035 related Products with: Norbormide-Based Probes and Their Application for Mitochondrial Imaging in Drosophila Melanogaster.

0.1ml (1mg/ml) 100ul 5 G50ul100ug Lyophilized1 mg0.1ml (1mg/ml)100ug Lyophilized0.1ml (1mg/ml) 100ul100 TESTS1mg

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#34118043   // To Up

Development of Mitochondria-Targeted Imaging Nanoplatforms by Incorporation of Fluorescent Carbon Quantum Dots.

Multifunctional nanoplatforms are promising scaffolds for biomedical applications such as bioimaging, chemical/biological sensors, drug delivery, and cancer diagnosis and/or treatments. Mitochondria play crucial roles in metabolism of eukaryotic cells; therefore, mitochondria-targeting molecule such as triphenylphosphonium (TPP) is attached onto the magnetic mesoporous silica nanoparticle ([email protected]). In order to track the nanoparticles, fluorescent carbon quantum dots (CDs) were conjugated to the [email protected] The as-constructed [email protected]/CQD nanoplatform showed minimal cytotoxicity in various cell lines such as A549, CHO, HeLa, SH-SY5Y, HFF, and HMEC-1. External magnetic field-assisted uptake of the nanoplatform by tumor cell has been achieved promptly. More importantly, conjugation with CQDs endows the nanoplatform multicolored fluorescence that can remain bright and stable inside cells for a long time. This nanoplatform provides a multifunctional platform in targeting, imaging, and agent delivery for mitochondria-related disease diagnosis and treatment.
Ye Zhang, Hong Bi

1015 related Products with: Development of Mitochondria-Targeted Imaging Nanoplatforms by Incorporation of Fluorescent Carbon Quantum Dots.

5 G5 mg250 mg1 g10 mg 25 G2x384 well plate1 mg 1 kit(s)

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#34118034   // To Up

Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes.

Surface modification of liposomes with a ligand is facilitated by the conjugation of the ligand to a hydrophobic molecule that serves to anchor the ligand to the liposomal bilayer. We describe here a simple protocol to conjugate a triphenylphosphonium group to several commercially available functionalized phospholipids. The resulting triphenylphosphonium-conjugated lipids can be used to prepare liposomes that preferentially associate with mitochondria when exposed to live mammalian cells in culture.
Parul Benien, Mohammed Almuteri, Shrey Shah, Mark Böhlke, Ahmed Mehanna, Gerard G M D'Souza

1784 related Products with: Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes.

25 G 5 G1,000 tests 500 ml 100μl1 kit1 mg1 mg100μg2000 IU 1 G

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#34117838   2021/06/12 To Up

Regenerating Critical Size Rat Segmental Bone Defects with a Self-Healing Hybrid Nanocomposite Hydrogel: Effect of Bone Condition and BMP-2 Incorporation.

The aim of the current study is to assess the biological performance of self-healing hydrogels based on calcium phosphate (CaP) nanoparticles and bisphosphonate (BP) conjugated hyaluronan (HA) in a critical size segmental femoral bone defect model in rats. Additionally, these hydrogels are loaded with bone morphogenetic protein 2 (BMP-2) and their performance is compared in healthy and osteoporotic bone conditions. Treatment groups comprise internal plate fixation and placement of a PTFE tube containing hydrogel (HA -CaP) or hydrogel loaded with BMP-2 in two dosages (HA -CaP-lowBMP2 or HA -CaP-highBMP2). Twelve weeks after bone defect surgery, bone formation is analyzed by X-ray examination, micro-CT analysis, and histomorphometry. The data show that critical size, segmental femoral bone defects cannot be healed with HA -CaP gel alone. Loading of the HA -CaP gel with low dose BMP-2 significantly improve bone formation and resulted in defect bridging in 100% of the defects. Alternatively, high dose BMP-2 loading of the HA -CaP gel does not improve bone formation within the defect area, but leads to excessive bone formation outside the defect area. Bone defect healing is not affected by osteoporotic bone conditions.
Claire I A van Houdt, Marianne K E Koolen, Paula M Lopez-Perez, Dietmar J O Ulrich, John A Jansen, Sander C G Leeuwenburgh, Harrie H Weinans, Jeroen J J P van den Beucken

1623 related Products with: Regenerating Critical Size Rat Segmental Bone Defects with a Self-Healing Hybrid Nanocomposite Hydrogel: Effect of Bone Condition and BMP-2 Incorporation.

200ul100ug Lyophilized100 μg100ug100ug100ug Lyophilized900 tests100ug10ìg

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#34117693   2021/06/11 To Up

Platelet-like particles reduce coagulopathy-related and neuroinflammatory pathologies post-experimental traumatic brain injury.

Coagulopathy may occur following traumatic brain injury (TBI), thereby negatively affecting patient outcomes. Here, we investigate the use of platelet-like particles (PLPs), poly(N-isopropylacrylamide-co-acrylic-acid) microgels conjugated with a fibrin-specific antibody, to improve hemostasis post-TBI. The objective of this study was to diminish coagulopathy in a mouse TBI model (controlled cortical impact) via PLP treatment, and subsequently decrease blood-brain barrier (BBB) permeability and neuroinflammation. Following an acute intravenous injection of PLPs post-TBI, we analyzed BBB permeability, ex vivo coagulation parameters, and neuroinflammation at 24 hr and 7 days post-TBI. Both PLP-treatment and control particle-treatment had significantly decreased BBB permeability and improved clot structure 24 hr post-injury. Additionally, no significant change in tissue sparing was observed between 24 hr and 7 days for PLP-treated cohorts compared to that observed in untreated cohorts. Only PLP-treatment resulted in significant reduction of astrocyte expression at 7 days and percent difference from 24 hr to 7 days. Finally, PLP-treatment significantly reduced the percent difference from 24 hr to 7 days in microglia/macrophage density compared to the untreated control. These results suggest that PLP-treatment addressed acute hypocoagulation and decreased BBB permeability followed by decreased neuroinflammation and fold-change tissue loss by 7 days post-injury. These promising results indicate that PLPs could be a potential therapeutic modality for TBI.
Jordan Todd, Vimala N Bharadwaj, Kimberly Nellenbach, Seema Nandi, Emily Mihalko, Connor Copeland, Ashley C Brown, Sarah E Stabenfeldt

2966 related Products with: Platelet-like particles reduce coagulopathy-related and neuroinflammatory pathologies post-experimental traumatic brain injury.

50 ug1.00 ml0.5mg10 mg96 tests100ug100 0.1 mg10ml50ug

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#34117664   2021/06/12 To Up

Nanographene-Osmapentalyne Complexes as a Cathode Interlayer in Organic Solar Cells Enhance Efficiency over 18.

Interface engineering is a critical method by which to efficiently enhance the photovoltaic performance of nonfullerene solar cells (NFSC). Herein, a series of metal-nanographene-containing large transition metal involving d -p conjugated systems by way of the addition reactions of osmapentalynes and p-diethynyl-hexabenzocoronenes is reported. Conjugated extensions are engineered to optimize the π-conjugation of these metal-nanographene molecules, which serve as alcohol-soluble cathode interlayer (CIL) materials. Upon extension of the π-conjugation, the power conversion efficiency (PCE) of PM6:BTP-eC9-based NFSCs increases from 16% to over 18%, giving the highest recorded PCE. It is deduced by X-ray crystallographic analysis, interfacial contact methods, morphology characterization, and carrier dynamics that modification of hexabenzocoronenes-styryl can effectively improve the short-circuit current density (J ) and fill factor of organic solar cells (OSCs), mainly due to the strong and ordered charge transfer, more matching energy level alignments, better interfacial contacts between the active layer and the electrodes, and regulated morphology. Consequently, the carrier transport is largely facilitated, and the carrier recombination is simultaneously impeded. These new CIL materials are broadly able to enhance the photovoltaic properties of OSCs in other systems, which provides a promising potential to serve as CILs for higher-quality OSCs.
Longzhu Liu, Shiyan Chen, Yangyang Qu, Xiang Gao, Liang Han, Zhiwei Lin, Liulin Yang, Wei Wang, Nan Zheng, Yongye Liang, Yuanzhi Tan, Haiping Xia, Feng He

1212 related Products with: Nanographene-Osmapentalyne Complexes as a Cathode Interlayer in Organic Solar Cells Enhance Efficiency over 18.

96 assays11 kit100 assays20 48 assays 1 kit400Tests100 assays100 assays100 assays

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