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#33080463   2020/10/15 To Up

Highly sensitive detection of Campylobacter spp. In chicken meat using a silica nanoparticle enhanced dot blot DNA biosensor.

Paper-based DNA biosensors are powerful tools in point-of-care diagnostics since they are affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high as required to enable DNA quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases the sensitivity of assays based on the use of biotinylated silica-nanoparticles (biotin-Si-NPs). After immobilization of a genomic Campylobacter DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP) in the presence of luminol and HO. Replacement of the single biotin by the biotin-Si-NPs boosted on average a 30 fold chemiluminescent read-out of the biosensor. Characterization of biotin-Si-NPs onto a paper with immobilized DNA was done using a scanning electron microscope. A limit of detection of 3 pg/μL of DNA, similar to the available qPCR kits, is achieved, but it is cheaper, easier and avoids inhibition of DNA polymerase by molecules from the food matrices. We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected Campylobacter from naturally contaminated chicken meat, without needing a PCR step. Hence, such an enhanced dot blot paves the path to the development of a portable and multiplex paper based platform for point-of-care screening of chicken carcasses for Campylobacter.
Priya Vizzini, Marisa Manzano, Carole Farre, Thierry Meylheuc, Carole Chaix, Nalini Ramarao, Jasmina Vidic

2791 related Products with: Highly sensitive detection of Campylobacter spp. In chicken meat using a silica nanoparticle enhanced dot blot DNA biosensor.

96 tests4 Membranes/Box50 assays2 Pieces/Box4 Arrays/Slide4 Membranes/Box2 Pieces/Box2 Pieces/Box4 Membranes/Box2 Pieces/Box4 Arrays/Slide

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#33080460   2020/09/18 To Up

Lymph-directed nitric oxide increases immune cell access to lymph-borne nanoscale solutes.

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Lauren F Sestito, Susan N Thomas

1317 related Products with: Lymph-directed nitric oxide increases immune cell access to lymph-borne nanoscale solutes.

100 2 x 96 well plate100

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#33080389   2020/10/17 To Up

Validation of steroid sulfates deconjugation for metabolic studies. Application to human urine samples.

Urinary sulfate fraction of the anabolic androgenic steroids is not analyzed routinely in anti-doping analyses but has demonstrated in the last years an increasing interest among the anti-doping community. Sulfate conjugates are linked to plasma proteins increasing the residence time in the body compared to glucuro-conjugated metabolites, and then their analyses may allow improving the detection time window of specific metabolites. Hydrolysis of sulfates can be made enzymatically or chemically and can be challenging, depending on the strategy selected.
D Martinez-Brito, M L Notarianni, M Iannone, X de la Torre, F Botrè

1781 related Products with: Validation of steroid sulfates deconjugation for metabolic studies. Application to human urine samples.

1 kit(96 Wells)100 TESTS1 kit(96 Wells)0.1 mg 100ul25 µg1 kit(96 Wells)0.1 mg0.1ml (1mg/ml) 100ul1 kit(96 Wells) 100ul

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#33080074   2020/10/20 To Up

MAIT Cell Dysregulation Correlates with Conjugated Bilirubin Level in Chronic Hepatitis B Virus Infection.

Mucosal-associated invariant T (MAIT) cells are non-conventional T cells restricted to MHC class I-related protein 1 (MR1). They are highly abundant in human liver, and activated by TCR-dependent and TCR-independent mechanisms to exhibit rapid innate-like effector responses. However, the roles of MAIT cells in chronic HBV infection still remain obscure. This study aims to test their antiviral potential, and investigate their dynamic changes and regulating factors during chronic HBV infection.
Yu Liu, Peng Zhu, Wei Wang, Xiaosheng Tan, Chuanqiao Liu, Yingshan Chen, Rongjuan Pei, Xue Cheng, Mi Wu, Qing Guo, Hongmei Liang, Zhihui Liang, Jia Liu, Yang Xu, Xiongwen Wu, Xiufang Weng

2331 related Products with: MAIT Cell Dysregulation Correlates with Conjugated Bilirubin Level in Chronic Hepatitis B Virus Infection.

100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100 μg100ug Lyophilized

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#33079558   2020/10/20 To Up

Enhanced Caspase-Mediated Abrogation of Autophagy by Temozolomide-Loaded and Panitumumab-Conjugated Poly(lactic--glycolic acid) Nanoparticles in Epidermal Growth Factor Receptor Overexpressing Glioblastoma Cells.

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Asmita Banstola, Ramesh Duwa, Fakhrosaddat Emami, Jee-Heon Jeong, Simmyung Yook

2216 related Products with: Enhanced Caspase-Mediated Abrogation of Autophagy by Temozolomide-Loaded and Panitumumab-Conjugated Poly(lactic--glycolic acid) Nanoparticles in Epidermal Growth Factor Receptor Overexpressing Glioblastoma Cells.

5 x 50 ug2 Pieces/Box50 ug100ug100ug100ug Lyophilized1 kit(96 Wells)100.00 ug100ug50 ug100ug100ug Lyophilized

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#33079536   2020/10/20 To Up

Low-Valent, Multiply Bonded, Trigonal-Planar Sb Complex: Rational Syntheses, Dual Acidic/Basic Properties, and Unexpected Semiconducting Characteristics.

A 4-center, 6π-conjugated, multiply bonded trigonal-planar complex, [Sb{Cr(CO)}] (), was synthesized via the hydride abstraction of [HSb{Cr(CO)}] () with HBF·HO, with the release of high yields of H. The oxidation state of the Sb atom in [EtN][] was well-defined as 0, which was evidenced by X-ray photoelectron spectroscopy and X-ray absorption near-edge structure. The distinct color-structure relationship of this low-valent Sb complex toward a wide range of organic solvents was demonstrated, as interpreted by time-dependent density functional theory calculations, allowing the trigonal-planar and the tetrahedral solvent adducts to be probed, revealing the dual acid/base properties of the Sb center. In addition, showed pronounced electrophilicity toward anionic and neutral nucleophiles, even with solvent molecules, to produce tetrahedral complexes [(Nu)Sb{Cr(CO)}] [; = 2, Nu = H, F, Cl, Br, I, OH; = 1, Nu = PEt, PPh, ,-dimethylformamide (DMF), acetonitrile (MeCN)]. On the contrary, the Fe/Cr hydride complex [HSb{Fe(CO)}{Cr(CO)}] () was obtained by treating with [HFe(CO)]. Upon hydride abstraction of with HBF·HO or [CPh][BF], a multiply bonded Fe/Cr trigonal-planar complex, [Sb{Fe(CO)}{Cr(CO)}] (), was produced in which the oxidation coupling Sb-containing complexes [SbCrFe(CO)] () and [HSbCrFe(CO)] () were yielded as final products. Complex exhibited dual Lewis acid/base properties via hydridation and protonation reactions, to form or , respectively. Surprisingly, [EtN][] possessed a low energy gap of 1.13 eV with an electrical conductivity in the range of (1.10-2.77) × 10 S·cm, showing that [EtN][] was a low-energy-gap semiconductor. The crystal packing, crystal indexing, and density of states results of [EtN][] further confirmed the efficient through-space conduction pathway via the intermolecular Sb···O(carbonyl) and O(carbonyl)···O(carbonyl) interactions of the 1D anionic zigzag chain of .
Minghuey Shieh, Yu-Huei Li, Chia-Hsien Lin, Tzu-Yen Sun

2253 related Products with: Low-Valent, Multiply Bonded, Trigonal-Planar Sb Complex: Rational Syntheses, Dual Acidic/Basic Properties, and Unexpected Semiconducting Characteristics.

5 IU1,000 tests10ug20 ul100 200 10ug1 mg100ug0.1 mg2

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#33079516   2020/10/20 To Up

Rapid, Ultrasensitive, and Quantitative Detection of SARS-CoV-2 Using Antisense Oligonucleotides Directed Electrochemical Biosensor Chip.

A large-scale diagnosis of the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) is essential to downregulate its spread within as well as across communities and mitigate the current outbreak of the pandemic novel coronavirus disease 2019 (COVID-19). Herein, we report the development of a rapid (less than 5 min), low-cost, easy-to-implement, and quantitative paper-based electrochemical sensor chip to enable the digital detection of SARS-CoV-2 genetic material. The biosensor uses gold nanoparticles (AuNPs), capped with highly specific antisense oligonucleotides (ssDNA) targeting viral nucleocapsid phosphoprotein (N-gene). The sensing probes are immobilized on a paper-based electrochemical platform to yield a nucleic-acid-testing device with a readout that can be recorded with a simple hand-held reader. The biosensor chip has been tested using samples collected from Vero cells infected with SARS-CoV-2 virus and clinical samples. The sensor provides a significant improvement in output signal only in the presence of its target-SARS-CoV-2 RNA-within less than 5 min of incubation time, with a sensitivity of 231 (copies μL) and limit of detection of 6.9 copies/μL without the need for any further amplification. The sensor chip performance has been tested using clinical samples from 22 COVID-19 positive patients and 26 healthy asymptomatic subjects confirmed using the FDA-approved RT-PCR COVID-19 diagnostic kit. The sensor successfully distinguishes the positive COVID-19 samples from the negative ones with almost 100% accuracy, sensitivity, and specificity and exhibits an insignificant change in output signal for the samples lacking a SARS-CoV-2 viral target segment (.., SARS-CoV, MERS-CoV, or negative COVID-19 samples collected from healthy subjects). The feasibility of the sensor even during the genomic mutation of the virus is also ensured from the design of the ssDNA-conjugated AuNPs that simultaneously target two separate regions of the same SARS-CoV-2 N-gene.
Maha Alafeef, Ketan Dighe, Parikshit Moitra, Dipanjan Pan

1765 related Products with: Rapid, Ultrasensitive, and Quantitative Detection of SARS-CoV-2 Using Antisense Oligonucleotides Directed Electrochemical Biosensor Chip.

250tests100tests2x96 well plate2x96 well plate200ug2x384 well plate250ul2x96 well plates100tests2x96 well plate250ul100tests

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#33079468   2020/10/20 To Up

Graphene Oxide "Surfactant"-Directed Tunable Concentration of Graphene Dispersion.

Homogeneous graphene dispersions with tunable concentrations are fundamental prerequisites for the preparation of graphene-based materials. Here, a strategy for effectively dispersing graphene using graphene oxide (GO) to produce homogeneous, tunable, and ultrahigh concentration graphene dispersions (>150 mg mL ) is proposed. The structure of GO with abundant edge-bound hydrophilic carboxyl groups and in-plane hydrophobic π-conjugated domains allows it to function as a special "surfactant" that enables graphene dispersion. In acidic solutions, GO sheets tend to form edge-to-edge hydrogen bonds and expose the π-conjugated regions which interact with graphene, thereby promoting graphene dispersion. While in alkaline solutions, GO sheets tend to stack in a surface-to-surface manner, thereby blocking the π-conjugated regions and impeding graphene dispersion. As the concentration of GO-dispersed graphene dispersion (GO/G) increases, a continuous transition between four states is obtained, including a dilute dispersion, a thick paste, a free-standing gel, and a kneadable, playdough-like material. Furthermore, GO/G can be applied to create desirable structures including highly conductive graphene films with excellent flexibility, thereby demonstrating an immense potential in flexible composite materials.
Jiajun Luo, Liangwei Yang, Danping Sun, Zhenfei Gao, Kun Jiao, Jin Zhang

2435 related Products with: Graphene Oxide "Surfactant"-Directed Tunable Concentration of Graphene Dispersion.

5 G1 mg 5 G0.5 mgKIT0.025 mg 100 G1 mg100 100ug Lyophilized100Tests5ml

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#33079456   2020/10/20 To Up

A Strategy for Tumor Targeting by Higher-Order Glycan Pattern Recognition: Synthesis and In Vitro and In Vivo Properties of Glycoalbumins Conjugated with Four Different N-Glycan Molecules.

Natural glycoconjugates that form glycocalyx play important roles in various biological processes based on cell surface recognition through pattern recognition mechanisms. This work represents a new synthesis-based screening strategy to efficiently target the cancer cells by higher-order glycan pattern recognition in both cells and intact animals (mice). The use of the very fast, selective, and effective RIKEN click reaction (6π-azaelectrocyclization of unsaturated imines) allows to synthesize and screen various structurally well-defined glycoalbumins containing two and eventually four different N-glycan structures in a very short time. The importance of glycan pattern recognition is exemplified in both cell- and mouse-based experiments. The use of pattern recognition mechanisms for cell targeting represents a novel and promising strategy for the development of diagnostic, prophylactic, and therapeutic agents for various diseases including cancers.
Ivan Smirnov, Regina Sibgatullina, Sayaka Urano, Tsuyoshi Tahara, Peni Ahmadi, Yasuyoshi Watanabe, Ambara R Pradipta, Almira Kurbangalieva, Katsunori Tanaka

1323 related Products with: A Strategy for Tumor Targeting by Higher-Order Glycan Pattern Recognition: Synthesis and In Vitro and In Vivo Properties of Glycoalbumins Conjugated with Four Different N-Glycan Molecules.

100ug100ug100ug100ug Lyophilized100ug Lyophilized100ug100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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