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Search results for: Cy3

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#35715613   2022/06/18 To Up

Preclinical evaluation and pilot clinical study of [F]AlF-NOTA-FAPI-04 for PET imaging of rheumatoid arthritis.

Fibroblast-like synoviocytes (FLSs) are key effector cells in the inflamed joints of patients with rheumatoid arthritis (RA). Previous studies have suggested that fibroblast activation protein (FAP) is highly expressed in RA-derived FLSs and is a specific marker of activated RA FLSs. In this study, we developed aluminum-[F]-labeled 1,4,7-triazacyclononane-N,N',N″-triacetic acid-conjugated FAP inhibitor 04 ([F]AlF-NOTA-FAPI-04) to image RA-FLSs in vitro and arthritic joints in collagen-induced arthritis (CIA) mice and RA patients.
Luna Ge, Zheng Fu, Yuchun Wei, Dandan Shi, Yun Geng, Huancai Fan, Ruojia Zhang, Yuang Zhang, Shufeng Li, Shijie Wang, Haojun Shi, Guanhua Song, Jihong Pan, Kai Cheng, Lin Wang

2619 related Products with: Preclinical evaluation and pilot clinical study of [F]AlF-NOTA-FAPI-04 for PET imaging of rheumatoid arthritis.

5 G10 lt10mg 1000 ml 10 mg

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#35700414   2022/06/14 To Up

Active Uptake and Trafficking of Nucleoside Triphosphates .

Modified nucleoside triphosphates (NTPs) are powerful probes and medicines, but their anionic character impedes membrane permeability. As such, invasive delivery techniques, transport carriers, or prodrug strategies are required for their use. Here, we present a fluorescent 2'-deoxyribonucleoside triphosphate "TAMRA-dATP" that exhibits surprisingly high bioavailability . TAMRA-dATP spontaneously forms nanoparticles in Mg-containing buffers that are taken into the vesicles of living cells and animals by energy-dependent processes. In cell cultures, photochemical activation with yellow laser light (561 nm) facilitated endosomal escape of TAMRA-dATP, resulting in its metabolic incorporation into DNA . In contrast, studies revealed that TAMRA-dATP is extensively trafficked by active pathways into cellular DNA of zebrafish () and where DNA labeling was observed in live animals, even without photochemical release. Metabolic labeling of DNA in whole, living animals can therefore be achieved by simply soaking animals in a buffer containing TAMRA-dATP or a structurally related compound, Cy3-dATP.
Verena N Schreier, Morten O Loehr, Evelyn Lattmann, Nathan W Luedtke

2094 related Products with: Active Uptake and Trafficking of Nucleoside Triphosphates .

2025 assays5 ug1 ml5 ug200ul5 ug25 assays1 unit1 kit(96 Wells)5 μg

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#35699591   2022/06/14 To Up

Radiopharmaceutical for detecting PSMA - positive metastatic colon cancer: Matched-pair comparison of 18F-BF3-Cy3-ACUPA and 68Ga-PSMA PET/MRI.

Prostate-specific membrane antigen (PSMA) - based radiopharmaceuticals are promising for the evaluation of PSMA-positive non-prostate cancers. In this case study, 18F-BF3-Cy3-ACUPA and 68Ga-PSMA positron emission tomography/magnetic resonance imaging (PET/MRI) were compared in a patient with metastatic colon cancer. Both 18F-BF3-Cy3-ACUPA and 68Ga-PSMA PET/MRI showed biopsy-proven metastatic left external iliac adenopathy, highlighting the feasibility of PSMA uptake in PET/MRI of metastatic nodal disease from colon cancer. Along with imaging evaluation, PSMA-based radiopharmaceuticals may also be used as a surrogate imaging tracer for potential theranostic applications using alpha or beta emitters in the context of PSMA-directed radiopharmaceutical therapy in advanced and progressive colorectal cancer.
Omer Aras, Cetin Demirdag, Harikrishna Kommidi, Richard Ting, Haluk B Sayman

2849 related Products with: Radiopharmaceutical for detecting PSMA - positive metastatic colon cancer: Matched-pair comparison of 18F-BF3-Cy3-ACUPA and 68Ga-PSMA PET/MRI.



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#35696870   2022/05/19 To Up

Fluorescent dendrimer-based probes for cell membrane imaging: Zebrafish epidermal labeling-based toxicity evaluation.

Visualizing the plasma membrane of living mammalian cells both in vitro and in vivo is crucial for tracking their cellular activities. However, due to the complex and dynamic nature of the plasma membrane, most commercial dyes for membrane staining can only realize very limited imaging performance. Thus, precise and stable plasma membrane imaging remains technically challenging. Here, by taking advantage of the small, well-defined, and amine-rich dendrimers, we prepared poly(ethylene glycol)-cholesterol (PEG-Chol)-conjugated and cyanine dye (e.g., cyanine2, cyanine3, and cyanine5)-labeled dendrimer nanoprobes (termed DPC-Cy2, DPC-Cy3, and DPC-Cy5 NPs). It was revealed that these probes enabled universal, wash-free, long-term (at least 8 h), and multicolor (green, yellow, and red) plasma membrane labeling of a variety of live mammalian cells. Further, we confirmed that the nanoprobes (using DPC-Cy5 as a representative) could achieve high-quality, wash-free, and stable cell surface labeling of live zebrafish embryos. More importantly, we demonstrated that our probes could act as biosensors to visualize the toxicity of metal-organic frameworks (MOFs) toward the epidermal cells of zebrafish embryos, and thus they hold great potential for identifying the toxic effect of drugs/materials at the single-cell scale or in live animals. The present work highlights the advantages of utilizing dendrimers for constructing functional imaging materials, and it is also believed that the fluorescent dendrimer nanoprobes developed in this work may find wide applications like cell imaging, drug toxicity evaluation, and cellular state monitoring.
Ke-Fei Xu, Hao-Ran Jia, Xiaoyang Liu, Ya-Xuan Zhu, Cong She, Junying Li, Qiu-Yi Duan, Rufeng Zhang, Fu-Gen Wu

2581 related Products with: Fluorescent dendrimer-based probes for cell membrane imaging: Zebrafish epidermal labeling-based toxicity evaluation.

Two 96-Well Microplate Ki10reactions One 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate KiTwo 96-Well Microplate Ki100tests10reactions One 96-Well Microplate Ki

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#35687263   2022/06/10 To Up

Role of M2 macrophages-derived extracellular vesicles in IL-1β-stimulated chondrocyte proliferation and inflammatory responses.

M2 macrophages-derived extracellular vesicles (M2-EVs) serve as a tool for the delivery of miRNAs and play an anti-inflammatory role in diseases. This study sought to explore the role of (M2-EVs) in the proliferation and inflammatory responses of IL-1β-stimulated chondrocytes. M2 macrophages were induced and characterized, followed by isolation and characterization of M2-EVs. Chondrocytes were treated with 10 ng/mL IL-1β and co-cultured with M2 macrophages transfected with Cy3-labeled miR-370-3p. Cell viability, TNF (tumor necrosis factor)-α, IL(Interleukin)-18, IL-10, miR-370-3p, and sex-determining region Y-related high-mobility-group box transcription factor 11 (SOX11) mRNA were determined via cell counting assay kit, colony formation, ELISA, and qRT-PCR. The binding relationship between miR-370-3p and SOX11 was testified via the dual-luciferase assay. The functional rescue experiment was designed to confirm the role of SOX11. M2-EVs improved chondrocyte viability and colony formation, lowered TNF-α and IL-18, and elevated IL-10. M2-EVs delivered miR-370-3p into chondrocytes to upregulate miR-370-3p. Upregulation of miR-370-3p in M2-EVs enhanced the protective role of M2-EVs in chondrocytes. miR-370-3p inhibited SOX11 transcription. SOX11 overexpression attenuated the protective role of M2-EVs in chondrocytes. Overall, our findings suggested that M2-EVs promote proliferation and suppress inflammatory responses in IL-1β-stimulated chondrocytes via the miR-370-3p/SOX11 axis.
Weiwei Guo, Li Su, Hao Zhang, Zhanhu Mi

2500 related Products with: Role of M2 macrophages-derived extracellular vesicles in IL-1β-stimulated chondrocyte proliferation and inflammatory responses.

5ug5ug2ug2ug2ug96 tests5 x 50 ug100 5ug96 tests5ug50 ug

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#35665468   2022/06/04 To Up

A ratiometric fluorescent aptamer homogeneous biosensor based on hairpin structure aptamer for AFB1 detection.

On the basis of aptamer (Apt) with hairpin structure and fluorescence resonance energy transfer (FRET), a ratio fluorescent aptamer homogeneous sensor was prepared for the determination of Aflatoxin B (AFB1). Initially, the Apt labeled simultaneously with Cy5, BHQ2, and cDNA labeled with Cy3 were formed a double-stranded DNA through complementary base pairing. The fluorescence signal of Cy3 and Cy5 were restored and quenched respectively. Thus, the ratio change of F to F was used to realized the detection of AFB1 with wider detection range and lower limit of detection (LOD). The response of the optimized protocol for AFB1 detection was wider linear range from 0.05 ng/mL to 100 ng/mL and the LOD was 12.6 pg/mL. The sensor designed in this strategy has the advantages of simple preparation and fast signal response. It has been used for the detection of AFB1 in labeled corn and wine, and has good potential for application in real samples.
Beibei Feng, Jing You, Fei Zhao, Min Wei, Yong Liu, Kun Yuan, Zhiguang Suo

2115 related Products with: A ratiometric fluorescent aptamer homogeneous biosensor based on hairpin structure aptamer for AFB1 detection.

1 LITRE96 tests2x96 well plate25 µg100tests2x96 well plates

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#35660893   2022/05/18 To Up

Bioinspired core-shell silica nanoparticles monitoring extra- and intra-cellular drug release.

Förster resonance energy transfer (FRET) has been widely used for monitoring drug release from nanoparticles (NPs). To understand the drug release from bioinspired drug-core silica-shell NPs, we synthesised two types of NPs using the dual-functional peptide SurSi via biosilicification for the first time, i.e., silica NP conjugated with FRET (Cy3 and Cy5) molecules, and FRET-core (DiO and DiI) silica-shell NP with different shell thicknesses (18 and 41 nm). The release kinetics of these two types of NPs were investigated under different conditions, including fetal bovine serum (FBS) and in cells, to mimic the drug release during blood circulation and intracellularly. Two different drug release mechanisms were identified. Cargo diffusion dominated the release during circulation, while the degradation of silica shell played a key role in drug release intracellularly.
Tengjisi, Yun Liu, Da Zou, Guangze Yang, Chun-Xia Zhao

1828 related Products with: Bioinspired core-shell silica nanoparticles monitoring extra- and intra-cellular drug release.

100tests100 rxns 100 mg100 µg2.50 nmol2000 rxns 10 mg100 100ul100 100ug Lyophilized

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#35652391   2022/06/02 To Up

Molecularly Precise, Bright, Photostable, and Biocompatible Cyanine Nanodots as Alternatives to Quantum Dots for Biomedical Applications.

Fluorescent imaging with fluorophores has become a powerful way to explore complex biological systems and visualize nanoparticles for drug delivery. However, it is challenging to develop fluorophores with ideal physical and optical properties. We report a method to synthesize cyanine nanodots with single-molecule structure, well-defined particle size, customizable fluorescent spectrum, bright and stable fluorescence. These cyanine nanodots are acquired by the divergent synthesis of cyanine dyes-cored polylysine (PLL) dendrimers. We demonstrated the feasibility of the method by synthesizing cyanine 3 (Cy3), cyanine 5 (Cy5), or cyanine 7 (Cy7) cored single-molecule nanodots up to eight generations with a size around 11 nm. We show these cyanine nanodots are capable of multiple biomedical applications, including multicolor cellular tracing and cancer imaging. These cyanine nanodots possess many merits of organic dots and quantum dots that are promising for future application.
Jiajia Yang, Kaiqi Wang, Yihuan Zheng, Ying Piao, Jinqiang Wang, Jianbin Tang, Youqing Shen, Zhuxian Zhou

1243 related Products with: Molecularly Precise, Bright, Photostable, and Biocompatible Cyanine Nanodots as Alternatives to Quantum Dots for Biomedical Applications.

1 kit50 assays1,000 tests0.2 mg1 kit(96 Wells)100 plates1 kit100 assays100 TESTS1 kit(96 Wells)

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#35598305   2022/05/22 To Up

Serotonin 5-HT and 5-HT receptor-mediated inhibition of glutamatergic transmission onto rat basal forebrain cholinergic neurones.

Cholinergic neurones in the basal forebrain (BF) project into various brain regions and receive excitatory inputs from the cortex and brain stem. These cholinergic neurones receive serotonergic fibres from the dorsal raphe nuclei. This study was aimed to elucidate serotonin (5-HT)-induced modulation of glutamatergic transmission onto rat BF cholinergic neurones identified with Cy3-192IgG. Excitatory postsynaptic currents (EPSCs) were evoked by focal stimulation. Bath application of either 5-HT, the 5-HT receptor agonist 8-OH-DPAT (DPAT), or the 5-HT receptor agonist CP93129 (CP), inhibited the amplitude of EPSCs. In the presence of both 5-HT and 5-HT receptor antagonists, the 5-HT-induced effect disappeared. The paired-pulse ratio (PPR) and coefficient of variation (CV) of the EPSCs were increased by CP, whereas DPAT had no effect on PPR or CV. DPAT inhibited the inward currents induced by puff application of l-glutamate, which were unaffected by CP. DPAT suppressed the amplitude of miniature EPSCs (mEPSCs) without affecting their frequency. CP decreased the frequency of mEPSCs in more than half of the neurones examined, whereas the amplitude was unaffected. DPAT or CP alone inhibited the NMDA receptor-mediated currents. 5-HT-induced inhibition of EPSCs was reduced in the presence of ω-agatoxin TK (Aga). Furthermore, CP-induced inhibition of EPSCs was eliminated in the presence of Aga. DPAT-induced inhibition of EPSCs was unchanged in the presence of Aga. These results suggest that activation of 5-HT receptors reduces the sensitivity of postsynaptic glutamate receptors to glutamate, whereas presynaptic activation of 5-HT receptors inhibits glutamate release by blocking P/Q-type calcium channels. KEY POINTS: We performed a patch-clamp study to investigate serotonin (5-HT)-induced modulation of glutamatergic transmission onto cholinergic neurones in the rat basal forebrain slices. Excitatory postsynaptic currents (EPSCs) were inhibited by 5-HT as well as agonists of 5-HT or 5-HT receptors. 5-HT-induced inhibition was antagonized by co-application of 5-HT and 5-HT receptor antagonists. The effects of 5-HT receptor agonists on the paired-pulse ratio, coefficient of variation of EPSCs, inward currents induced by puff application of l-glutamate as well as miniature EPSCs suggest that activation of 5-HT receptors decreases the sensitivity of postsynaptic glutamate receptors to glutamate, whereas 5-HT receptors presynaptically inhibit glutamate release. The 5-HT agonist-induced inhibition was eliminated in the presence of a P/Q-type calcium channel blocker, whereas the 5-HT agonist still inhibited the EPSCs even in the presence of the blocker. The present study reveals different pre- and postsynaptic mechanisms underlying 5-HT and 5-HT receptor-mediated modulation of excitatory transmission.
Takuma Nishijo, Etsuko Suzuki, Toshihiko Momiyama

2658 related Products with: Serotonin 5-HT and 5-HT receptor-mediated inhibition of glutamatergic transmission onto rat basal forebrain cholinergic neurones.

100 μg100 μg100 μg100ug50 ug 200ug100 µg100 100ul100ug

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#35489746   // To Up

Expression of Is Up-regulated in Low-grade Glioma and Positively Correlated With Meningioma Grade.

α-Enolase (ENO1) is a glycolytic enzyme involved in the Warburg effect which cancer cells utilize to satisfy their higher need for nutrients. Up-regulation of ENO1 has been detected in several tumor types, including melanoma and endometrial, gastric and colorectal cancer. In these tumors, ENO1 may function as prognostic marker. Therefore, it was our interest to determine the expression of ENO1 in glioma and meningioma and whether chemotherapy of glioma alters ENO1 expression.
Dang Thuy Diem Dinh, Saskia Kuhl, Lukas Görtz, Roland Goldbrunner, Marco Timmer

2188 related Products with: Expression of Is Up-regulated in Low-grade Glioma and Positively Correlated With Meningioma Grade.

20x50 ug

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