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#34678651   2021/10/13 To Up

Metal-organic frameworks-assisted nonenzymatic cascade amplification multiplexed strategy for sensing acute myocardial infarction related microRNAs.

Amplification strategies for multiple microRNAs (miRNAs) detection are pivotal for acute myocardial infarction (AMI). Herein, we rationally developed a metal-organic frameworks-assisted nonenzymatic cascade amplification strategy for simultaneous quantification of three AMI-related miRNAs (miR-21, miR-499 and miR-133a). The fluorescence of the elaborately designed DNA molecular beacons with the respective modification of FAM, TAMRA and Cy5 in the terminal was quenched by a metal-organic framework named Fe-MIL-88. When targets miRNA appeared, they hybridized with the corresponding DNA molecular beacons, and the catalyzed hairpin assembly (CHA) reaction would be triggered, producing "Y" shaped three-branched duplex nanostructure with the targets released, and initiating subsequent another cycle. The "Y" shaped nanostructures could not be adsorbed onto the surface of Fe-MIL-88 due to the weaker affinity between Fe-MIL-88 and "Y" shaped nanostructures. Therefore, the fluorescence of "Y" shaped nanostructures could not be quenched by Fe-MIL-88. In this way, three AMI-related miRNAs were simultaneously detected in the respective ranges of 0.05-30 nM, 0.08-30 nM and 0.1-20 nM with respective limits of detection down to 13, 25 and 40 pM. Furthermore, the method was successfully employed to determine three AMI-related miRNAs in human serum. The strategy offered great opportunity for ultrasensitive detecting multiple AMI-related miRNAs and substantially improving the accuracy of clinical early AMI diagnosis.
Xia Cheng, Dandan Ren, Guanhong Xu, Fangdi Wei, Jing Yang, Jian Xu, Lin Wang, Qin Hu, Yao Cen

2702 related Products with: Metal-organic frameworks-assisted nonenzymatic cascade amplification multiplexed strategy for sensing acute myocardial infarction related microRNAs.

100ug5 g 6 ml Ready-to-use 100ug25 μg2 Pieces/Box 100ul 100 G1kit100 mg

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#34677979   2021/10/22 To Up

MR/NIRF Dual-Mode Imaging of αvβ3 Integrin-Overexpressing Tumors Using a Lipopeptide-Based Contrast Agent.

Early diagnosis and noninvasive detection of hepatocellular carcinoma have profound clinical implications for treatment quality and improved prognosis. To obtain high-resolution macroscopic anatomical information and high-sensitivity microscopic optical signals to detect tumors, it is highly desirable to develop dual-mode magnetic resonance imaging (MRI) and near-infrared fluorescent (NIRF) probes. An MR/NIRF dual-mode targeted contrast agent was created by encapsulating cyclic arginine-glycine-aspartate (cRGD) and Cy5.5 in liposomes and characterized by the particle size distribution, cytotoxicity, targeting, and MRI relaxivity. The MR T2 intensity and fluorescence intensity were evaluated in the tumors, livers, and muscles after the injection of cRGD-Liposome-Cy5.5 and Liposome-Cy5.5 at different time points. The average size of cRGD-Liposome-Cy5.5 was 62.33 ± 4.648 nm. The transverse relaxivity (R2) values had a negative correlation with the concentration of molecular probes. The MR signal intensity was enhanced in tumors after the cRGD-Liposome-Cy5.5 injection and not enhanced in liver parenchyma and muscles at the same time. The fluorescence intensity was enhanced in tumors after cRGD-Liposome-Cy5.5 injection in the targeted group. cRGD -Liposome-Cy5.5 as an entirely organic T2-positive dual-mode MR/NIRF targeted contrast agent is therefore able to detect early-stage hepatocellular carcinoma by targeting integrin αvβ3, providing advantages for potential clinical utility and ease of clinical transformation.
Qi Wang, Lei Huang, Xuna Zhu, Yan Zhou, Jialing Wang, Danke Su, Lidong Liu

2530 related Products with: MR/NIRF Dual-Mode Imaging of αvβ3 Integrin-Overexpressing Tumors Using a Lipopeptide-Based Contrast Agent.

0.1 mg100ug100ug100ug Lyophilized100ug 100ul100.00 ug100ug Lyophilized100ug Lyophilized100ug100ug100tests

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#34672583   2021/10/21 To Up

Hydrogen Bond Engineering Visualized by Picometer-Level Distortion of Planar Porphyrin Isomers.

Directly investigating hydrogen bond (HB) dynamics in molecular materials is a challenging task. Here, we report a set of porphyrin isomers, porphycenes, that visualize slight changes on the order of picometers in the intramolecular HB dynamics. Intramolecular HBs of porphycenes were regulated by the systematic modification at meso positions with methyl (Me), cyclopentyl (Cy5), and cyclohexyl (Cy6) moieties. Notably, the quantum yields varied from 35 to 0.04% in chloroform, depending on a slight distortion in the porphycene framework. SC-XRD, XPS, and NMR clearly revealed that the Me and Cy6 moieties increased the nonradiative deactivation by strengthening the intramolecular NH···N HBs whereas Cy5 retained their photoluminescence properties. This is the first example of how the distortion of planar porphyrinoids at the picometer level along with the strength of the intramolecular NH···N HBs can drastically affect their optical properties. The results revealed new avenues of HB engineering based on porphyrinoids.
Daiki Koga, Toshikazu Ono, Hyuga Shinjo, Yoshio Hisaeda

2255 related Products with: Hydrogen Bond Engineering Visualized by Picometer-Level Distortion of Planar Porphyrin Isomers.

1 g 1KG1 g10 mg0.5 mg 100 G1 kit5 mg 1KG100 mg1 g

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#34672523   2021/10/21 To Up

Development of a Single Quantum Dot-Mediated FRET Nanosensor for Sensitive Detection of Single-Nucleotide Polymorphism in Cancer Cells.

Single-nucleotide polymorphisms (SNPs) are important hallmarks of human diseases. Herein, we develop a single quantum dot (QD)-mediated fluorescence resonance energy transfer (FRET) nanosensor with the integration of multiple primer generation rolling circle amplification (MPG-RCA) for sensitive detection of SNPs in cancer cells. This assay involves only a linear padlock probe for MPG-RCA. The presence of a mutant target facilitates the circularization of linear padlock probes to initiate RCA, producing three short single-stranded DNAs (ssDNAs) with the assistance of nicking endonuclease. The resulting ssDNAs can function as primers to induce cyclic MPG-RCA, resulting in the exponential amplification and generation of large numbers of linker probes. The linker probes can subsequently hybridize with the Cy5-labeled reporter probes and the biotinylated capture probes to obtain the sandwich hybrids. The assembly of these sandwich hybrids on the 605 nm-emission quantum dot (605QD) generates the 605QD-oligonucleotide-Cy5 nanostructures, resulting in efficient FRET from the 605QD to Cy5. This nanosensor is free from both the complicated probe design and the exogenous primers and has distinct advantages of high amplification efficiency, zero background signal, good specificity, and high sensitivity. It can detect SNPs with a large dynamic range of 8 orders of magnitude and a detection limit of 5.41 × 10 M. Moreover, this nanosensor can accurately distinguish as low as 0.001% mutation level from the mixtures, which cannot be achieved by previously reported methods. Furthermore, it can discriminate cancer cells from normal cells and even quantify SNP at the single-cell level.
Chen-Chen Li, Jinping Hu, Xiliang Luo, Juan Hu, Chun-Yang Zhang

2279 related Products with: Development of a Single Quantum Dot-Mediated FRET Nanosensor for Sensitive Detection of Single-Nucleotide Polymorphism in Cancer Cells.

1.00 flask0.1mg

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#34662822   2021/10/15 To Up

Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.

The anticoagulant agent recombinant thrombomodulin (rTM) activates protein C to prevent excessive coagulation and also possibly regulates hyper-inflammation via neutralization of high-mobility-group B1 (HMG-B1). The glycocalyx layer in endothelial cells also plays a pivotal role in preventing septic shock-associated hyperpermeability. The present study examined the effect of rTM in a murine model of Streptococcus pneumoniae-induced sepsis.
Eizo Watanabe, Toshinobu Akamatsu, Masaaki Ohmori, Mayu Kato, Noriko Takeuchi, Naruhiko Ishiwada, Rintaro Nishimura, Haruka Hishiki, Lisa Fujimura, Chizuru Ito, Masahiko Hatano

1580 related Products with: Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.

96 assays 100ul4 Sample Kit100 ul8 Sample Kit100 ug10 50 1-8 Sample Kit

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#34655679   2021/10/13 To Up

A common strategy to improve transmembrane transport in polarized epithelial cells based on sorting signals: Guiding nanocarriers to TGN rather than to the basolateral plasma membrane directly.

The intestinal barrier has always been the rate-limiting step in the oral administration process. To overcome the intestinal barrier, researchers have widely adopted nanocarriers, especially active-targeting nanocarriers strategies. However, most of these strategies focus on the ligand decoration of nanocarriers targeting specific receptors, so their applications are confined to specific receptors or specific cell types. In this study, we tried to investigate more common strategies in the field of transmembrane transport enhancement. Trans-Golgi network (TGN) is the sorting center of biosynthetic route which could achieve polarized localization of proteins in polarized epithelial cells, and the basolateral plasma membrane is where all transcytotic cargos have to pass through. Thus, it is expected that guiding nanocarriers to TGN or basolateral plasma membrane may improve the transcytosis. Hence, we choose sorting signal peptide to modify micelles to guide micelles to TGN (named as BAC decorated micelles, BAC-M) or to basolateral plasma membrane (named as STX decorated micelles, STX-M). By incorporating coumarin-6 (C6) or Cy5-PEG-PCL in the micelles to indicate the behavior of micelles, the effects of these two strategies on the transcytosis were investigated. To our surprise, BAC-M and STX-M behaved quite differently when crossing biological barriers. BAC-M showed significant superiority in colocalization with TGN, transmembrane transport and even in vivo absorption, while STX-M had no significant difference from blank micelles. Further investigation revealed that the strategy of directly guiding nanocarriers to the basolateral plasma membrane (STX-M) only caused the stack of vesicles near the basolateral plasma membrane. So, we concluded that guiding nanocarriers to TGN which related to secretion may contribute to the transmembrane transport. This common strategy based on the physiological function of TGN in polarized epithelial cells will have broad application prospects in overcoming biological barrier.
Runyu Zhang, Hailiang Deng, Yuxing Lin, Xing Wang, Bing He, Wenbing Dai, Hua Zhang, Ying Zheng, Qiang Zhang, Xueqing Wang

2304 related Products with: A common strategy to improve transmembrane transport in polarized epithelial cells based on sorting signals: Guiding nanocarriers to TGN rather than to the basolateral plasma membrane directly.

1 kit1 kit100 extractions96T100 25 100 μg

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#34651630   2021/10/27 To Up

Critical role of H-aggregation for high-efficiency photoinduced charge generation in pristine pentamethine cyanine salts.

The mechanism of photoinduced symmetry-breaking charge separation in solid cyanine salts at the base of organic photovoltaic and optoelectronic devices is still debated. Here, we employ femtosecond transient absorption spectroscopy (TAS) to monitor the charge transfer processes occurring in thin films of pristine pentamethine cyanine (Cy5). Oxidized dye species are observed in Cy5-hexafluorophosphate salts upon photoexcitation, resulting from electron transfer from monomer excited states to H-aggregates. The charge separation proceeds with a quantum yield of 86%, providing the first direct proof of high efficiency intrinsic charge generation in organic salt semiconductors. The impact of the size of weakly coordinating anions on charge separation and transport is studied using TAS alongside electroabsorption spectroscopy and time-of-flight techniques. The degree of H-aggregation decreases with increasing anion size, resulting in reduced charge transfer. However, there is little change in carrier mobility, as despite the interchromophore distance increasing, the decrease in energetic disorder helps to alleviate the trapping of charges by H-aggregates.
George C Fish, Juan Manuel Moreno-Naranjo, Andreas Billion, Daniel Kratzert, Erwin Hack, Ingo Krossing, Frank Nüesch, Jacques-E Moser

1031 related Products with: Critical role of H-aggregation for high-efficiency photoinduced charge generation in pristine pentamethine cyanine salts.

96 wells (1 kit) 1 G400Tests96 wells (1 kit)

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#34648222   2021/10/14 To Up

Emerging Design Principle of Near-Infrared Upconversion Sensitizer Based on Mitochondria-Targeted Organic Dye for Enhanced Photodynamic Therapy.

Upconversion luminescent (UCL) triggered photodynamic therapy (PDT) affords superior outcome for cancer treatment. However, conventional UCL materials which all work as multiphoton absorption (MPA) process, inevitably need extremely high power density far over the maximum permissible exposure (MPE) to laser. Here, an one-photon absorption molecular upconversion sensitizer Cy5.5-Br based on frequency upconversion luminescent (FUCL) is designed for PDT. The unusual super heavy atom effect (SHAE) in Cy5.5-Br strongly enhance its spin-orbit coupling (0.23 cm-1), triplet quantum yield (11.1%) and triplet state lifetime (18.8 μs) while the potential hot-band absoption of Cy5.5-Br are well maintained. Importantly, Cy5.5-Br can efficiently target to the tumour site and kill cancer cells by destroying mitochondria under a biosafety MPE to 808 nm laser. The photostability and antitumor results are obviously superior to that of Stoke process. This work first provide a design criterion for FUCL dyes to realize effective PDT upon a biosafety optical density, possibly being more clinical benefits than conventional MPA materials.
Ruisong Tian, Chao Wang, Weijie Chi, Jiangli Fan, Jianjun Du, Saran Long, Lianying Guo, Xiaogang Liu, Xiaojun Peng

2332 related Products with: Emerging Design Principle of Near-Infrared Upconversion Sensitizer Based on Mitochondria-Targeted Organic Dye for Enhanced Photodynamic Therapy.

20 ug1 mg 125 ml 0.2 mg10 μg2.5 mg1 mg50 assays96 rxns25 assays

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