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Search results for: Cy7

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#32705686   2020/07/23 To Up

Near-infrared dye-labeled antibody COC183B2 enables detection of tiny metastatic ovarian cancer and optimizes fluorescence-guided surgery.

We aimed to evaluate the ability of the fluorescent monoclonal antibody probe COC183B2-Cy7 (Cy7-conjugated COC183B2 antibody) to detect tiny metastatic lesions of ovarian cancer and thus guide precise tumor resection.
Junchen Chen, Yanxiu Guo, Hui Li, Chen Zhang, Xiaohong Chang, Ruiqiong Ma, Hongyan Cheng, Xue Ye, Heng Cui, Yi Li

1541 related Products with: Near-infrared dye-labeled antibody COC183B2 enables detection of tiny metastatic ovarian cancer and optimizes fluorescence-guided surgery.

96 tests96 tests50 ug 8 Array supply x 2100ug100ug

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#32697591   2020/07/22 To Up

Deciphering the Structure-Property Relations in Substituted Heptamethine Cyanines.

Heptamethine cyanines (Cy7) are fluorophores essential for modern bioimaging techniques and chemistry. Here, we systematically evaluated the photochemical and photophysical properties of a library of Cy7 derivatives containing diverse substituents in different positions of the heptamethine chain. A single substitution allows modulation of their absorption maxima in the range of 693-805 nm and photophysical properties, such as quantum yields of singlet-oxygen formation, decomposition, and fluorescence or affinity to singlet oxygen, within 2-3 orders of magnitude. The same substituent in different positions of the chain often exhibits distinctly contradictory effects, demonstrating that both the type and position of the substituent are pivotal for the design of Cy7-based applications. The combination of experimental results with quantum-chemical calculations provides insights into the structure-property relationship, the elucidation of which will accelerate the development of cyanines with properties tailored for specific applications, such as fluorescent probes and sensors, photouncaging, photodynamic therapy, or singlet-oxygen detection.
Lenka Štacková, Eva Muchová, Marina Russo, Petr Slavíček, Peter Štacko, Petr Klán

1496 related Products with: Deciphering the Structure-Property Relations in Substituted Heptamethine Cyanines.

1100 μg25

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#32598578   2020/06/29 To Up

The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.

Anti-CD20 monoclonals (MoAbs) are used in a variety of autoimmune disorders. The aim is to eliminate memory B cells sustaining the tissue damage and the production of pathogenic autoantibodies, while preserving naïve cells. The disappearance of memory B cells and the repopulation by naïve cells correlate with good clinical response, while the reappearance of memory B cells and plasmablasts correlates with relapse or resistance to therapy. Anti-CD20 induce extremely low B cell levels, requiring high-resolution techniques. The immune monitoring protocol developed by ISCCA is described and validated, to provide a standardized method for the clinical decision-making process during anti-CD20 therapies in autoimmune diseases.
Arianna Gatti, Francesco Buccisano, Maria T Scupoli, Bruno Brando

1852 related Products with: The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.

100ug Lyophilized1 ml10.1 mg100ug Lyophilized100ug Lyophilized100

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#32558120   2020/06/17 To Up

A Complex Comprising a Cyanine Dye Rotaxane and a Porphyrin Nanoring as a Model Light-Harvesting System.

A nanoring-rotaxane supramolecular assembly with a Cy7 cyanine dye (hexamethylindotricarbocyanine) threaded along the axis of the nanoring was synthesized as a model for the energy transfer between the light-harvesting complex LH1 and the reaction center in purple bacteria photosynthesis. The complex displays efficient energy transfer from the central cyanine dye to the surrounding zinc porphyrin nanoring. We present a theoretical model that reproduces the absorption spectrum of the nanoring and quantifies the excitonic coupling between the nanoring and the central dye, thereby explaining the efficient energy transfer and demonstrating similarity with structurally related natural light-harvesting systems.
Jiratheep Pruchyathamkorn, William J Kendrick, Andrew T Frawley, Andrea Mattioni, Felipe Caycedo-Soler, Susana F Huelga, Martin B Plenio, Harry L Anderson

1098 related Products with: A Complex Comprising a Cyanine Dye Rotaxane and a Porphyrin Nanoring as a Model Light-Harvesting System.

1 kit(96 Wells)100 assays1 kit1 kit720/kit1 kit(96 Wells)1 x 96 assays

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#32548514   2020/05/28 To Up

Near-Infrared Dual-Emission Ratiometric Fluorescence Imaging Nanoprobe for Real-Time Tracing the Generation of Endogenous Peroxynitrite in Single Living Cells and In Vivo.

Peroxynitrite (ONOO) is a highly reactive nitrogen species with potent oxidant and nitrating properties. Its excessive generation can cause DNA and protein damage, thereby contributing to cell injury, and it is closely related to the development of many diseases. Thus, there is an urgent need for a reliable method to determine changes in the steady-state levels of ONOO in vivo. Ratiometric imaging, due to its built-in self-calibration system, can reduce artifacts and enable reliable in vivo imaging. In this study, we designed and prepared near-infrared (NIR) biomass quantum dots (NI-BQDs) and covalently coupled them with the NIR dye Cyanine7 (Cy7) to construct an NIR dual-emission nanoprobe (NI-BQD-Cy7) for real-time tracing the generation of endogenous ONOO in single living cells and in vivo by ratiometric fluorescence imaging. NI-BQD-Cy7 exhibited high detection sensitivity and selectivity for ONOO in the mitochondria. Additionally, it can produce dual NIR fluorescence emission, thus allowing in situ ratiometric fluorescence imaging to real-time trace the generation and concentration changes of ONOO in vivo. The application of the proposed NIR dual-emission nanoprobe can provide accurate information for the study of the biological function of ONOO in single living cells and in vivo, and it is very useful to explain the mechanism of cell damage caused by ONOO.
Pengxiang Lin, Dongxia Chen, Liangliang Zhang, Jiayao Xu, Yong Huang, Shulin Zhao

1536 related Products with: Near-Infrared Dual-Emission Ratiometric Fluorescence Imaging Nanoprobe for Real-Time Tracing the Generation of Endogenous Peroxynitrite in Single Living Cells and In Vivo.

251 kit1 kit251 mg1 kit251 mg1 mg25

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#32505033   2020/05/27 To Up

A novel nanoparticle-based theranostic agent targeting LRP-1 enhances the efficacy of neoadjuvant radiotherapy in colorectal cancer.

Neoadjuvant radiotherapy has become an important therapeutic option for colorectal cancer (CRC) patients, whereas complete tumor response is observed only in 20-30% patients. Therefore, the development of diagnostic probe for radio-resistance is important to decide an optimal treatment timing and strategy for radiotherapy-resistant CRC patients. In this study, using the patient-derived xenograft (PDX) mouse model established with a radio-resistant CRC tumor tissue, we found low-density lipoprotein receptor-related protein-1 (LRP-1) as a radio-resistant marker protein induced by initial-dose radiation in radio-resistant CRC tumors. Simultaneously, we discovered a LRP-1 targeting peptide in a radio-resistant CRC PDX through in vivo peptide screening. We next engineered the theranostic agent made of human serum albumin nanoparticles (HSA NPs) containing 5-FU for chemo-radiotherapy and decorating LRP-1-targeting peptide for tumor localization, Cy7 fluorophore for diagnostic imaging. The nanoparticle-based theranostic agent accurately targeted the tumor designated by LRP-1 responding radiation and showed dramatically improved therapeutic efficacy in the radio-resistant PDX model. In conclusion, we have identified LRP-1 as a signature protein of radio-resistant CRC and successfully developed LRP-1-targeting HSA-NP containing 5-FU that is a novel theranostic tool for both diagnostic imaging and neoadjuvant therapy of CRC patients. This approach is clinically applicable to improve the effectiveness of neo-adjuvant radiotherapy and increase the ratio of complete tumor response in radio-resistant CRC.
Kyoung Jin Lee, Eun Jung Ko, Yun-Yong Park, Seok Soon Park, Eun Jin Ju, Jin Park, Seol Hwa Shin, Young-Ah Suh, Seung-Mo Hong, In Ja Park, Kyu-Pyo Kim, Jung Jin Hwang, Se Jin Jang, Jung Shin Lee, Si Yeol Song, Seong-Yun Jeong, Eun Kyung Choi

1075 related Products with: A novel nanoparticle-based theranostic agent targeting LRP-1 enhances the efficacy of neoadjuvant radiotherapy in colorectal cancer.

100ul100ug Lyophilized100ug100ug

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#32490374   2020/05/16 To Up

Polymeric micelles loaded with carfilzomib increase tolerability in a humanized bone marrow-like scaffold mouse model.

Carfilzomib-loaded polymeric micelles (CFZ-PM) based on poly(ethylene glycol)-b-poly(N-2-benzoyloxypropyl methacrylamide) (mPEG-b-p(HPMA-Bz)) were prepared with the aim to improve the maximum tolerated dose of carfilzomib in a "humanized" bone marrow-like scaffold model. For this, CFZ-PM were prepared and characterized for their size, carfilzomib loading and cytotoxicity towards multiple myeloma cells. Further, circulation and tumor & tissue distribution of fluorescently labeled micelles were determined. Tolerability of CFZ-PM the clinical approved formulation - Kyprolis® - was assessed. CFZ-PM presented small diameter below 55 nm and low PDI < 0.1. Cy7-labeled micelles circulated for extended periods of time with over 80% of injected dose in circulation at 24 h after intravenous injection and 1.3% of the injected dose of Cy7-labeled micelles accumulated in myeloma tumor-bearing scaffolds. Importantly, CFZ-PM were well tolerated whereas Kyprolis® showed adverse effects. Kyprolis® dosed at the maximum tolerated dose, as well as CFZ-PM, did not show therapeutic benefit, while multiple myeloma cells showed sensitivity , underlining the importance of the bone marrow crosstalk in testing novel formulations. Overall, this work indicates that PM are potential drug carriers of carfilzomib.
Aida Varela-Moreira, Demian van Straten, Heleen F van Leur, Ruud W J Ruiter, Anil K Deshantri, Wim E Hennink, Marcel H A M Fens, Richard W J Groen, Raymond M Schiffelers

1167 related Products with: Polymeric micelles loaded with carfilzomib increase tolerability in a humanized bone marrow-like scaffold mouse model.

500 100ug1 mg100 μg4 Arrays/Slide1 mg100 μg100 μg100 μg100.00 ug

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#32440115   2020/05/04 To Up

Primary Studies on Construction and Evaluation of Ion-Sensitive in situ Gel Loaded with Paeonol-Solid Lipid Nanoparticles for Intranasal Drug Delivery.

Paeonol (PAE) is a potential central neuroprotective agent with poor water solubility and rapid metabolism in vivo. The key to improve the clinical application of PAE in the treatment of neurodegenerative diseases is to improve the brain delivery of it. The purpose of this study was to construct a paeonol-solid lipid nanoparticles-in situ gel (PAE-SLNs-ISG) drug delivery system based on nose-brain transport pathway.
Yue Sun, Lingjun Li, Huichao Xie, Yuzhen Wang, Shuang Gao, Li Zhang, Fumin Bo, Shanjing Yang, Anjie Feng

1689 related Products with: Primary Studies on Construction and Evaluation of Ion-Sensitive in situ Gel Loaded with Paeonol-Solid Lipid Nanoparticles for Intranasal Drug Delivery.

100 assays100 assays 1 G100 assays5 mg100ug100 assays5 mg100 assays5 mg

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#32436301   2020/05/21 To Up

Immunization with cytolethal distending toxin B produces autoantibodies to vinculin and small bowel bacterial changes in a rat model of postinfectious irritable bowel syndrome.

Recent data substantiate the importance of acute gastroenteritis in the development of irritable bowel syndrome (IBS). An animal model of postinfectious IBS determined the importance of cytolethal distending toxin B (CdtB) during live Campylobacter jejuni infection and its development of autoimmunity to vinculin. In this study, we examine whether subcutaneous exposure to CdtB alone is sufficient to produce the postinfectious IBS effect and autoimmunity.
Walter Morales, Konstantinos Triantafyllou, Gonzalo Parodi, Stacy Weitsman, Sung Chul Park, Ali Rezaie, Nipaporn Pichetshote, Eugenia Lin, Mark Pimentel

2632 related Products with: Immunization with cytolethal distending toxin B produces autoantibodies to vinculin and small bowel bacterial changes in a rat model of postinfectious irritable bowel syndrome.

100 UG100 100 ul900 tests0.5 ml1 mL200 1 mg0.1ml (1.3mg/ml)1 mg

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#32409881   2020/05/14 To Up

Fluorescence labeling of a Na1.7-targeted peptide for near-infrared nerve visualization.

Accidental peripheral nerve injury during surgical intervention results in a broad spectrum of potentially debilitating side effects. Tissue distortion and poor visibility can significantly increase the risk of nerve injury with long-lasting consequences for the patient. We developed and characterized Hs1a-FL, a fluorescent near-infrared molecule for nerve visualization in the operating theater with the aim of helping physicians to visualize nerves during surgery. Hs1a was derived from the venom of the Chinese bird spider, Haplopelma schmidti, and conjugated to Cy7.5 dye. Hs1a-FL was injected intravenously in mice, and harvested nerves were imaged microscopically and with epifluorescence.
Junior Gonzales, Giacomo Pirovano, Chun Yuen Chow, Paula Demetrio de Souza Franca, Lukas M Carter, Julie K Klint, Navjot Guru, Jason S Lewis, Glenn F King, Thomas Reiner

1293 related Products with: Fluorescence labeling of a Na1.7-targeted peptide for near-infrared nerve visualization.

1 kit1 kit1 kit5 g25 g1 kit1 kit1 kit1 kit5 g

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