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#34129942   2021/06/12 To Up

Trimethylacetic anhydride-based derivatization facilitates quantification of histone marks at the MS1 level.

Histone post-translational modifications (hPTMs) are epigenetic marks that strongly affect numerous processes, including cell cycling and protein interactions. They have been studied by both antibody- and mass spectrometry-based methods for years, but the analyses are still challenging, mainly due to the diversity of histones and their modifications arising from high contents of reactive amine groups in their amino acid sequences. Here, we introduce use of trimethylacetic anhydride (TMA) as a new reagent for efficient histone derivatization, which is a requirement for bottom-up proteomic hPTM analysis. TMA can derivatize unmodified amine groups of lysine residues and amine groups generated at peptide N-termini by trypsin digestion. The derivatization is facilitated by microwave irradiation, which also reduces incubation times to minutes. We demonstrate that histone derivatization with TMA reliably provides high yields of fully derivatized peptides, and thus is an effective alternative to conventional methods. TMA afforded more than 98 % and 99 % labeling efficiencies for histones H4 and H3, respectively, thereby enabling accurate quantification of peptide forms. Trimethylacetylation substantially improves chromatographic separation of peptide forms, which is essential for direct quantification based on signals extracted from MS1 data. For this purpose, software widely applied by the proteomics community can be used without additional computational development. Thorough comparison with widely applied propionylation highlights the advantages of TMA-based histone derivatization for monitoring hPTMs in biological samples.
Hana Kuchaříková, Pavlína Dobrovolná, Gabriela Lochmanová, Zbyněk Zdráhal

2820 related Products with: Trimethylacetic anhydride-based derivatization facilitates quantification of histone marks at the MS1 level.



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#34129836   2021/06/08 To Up

Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration.

During development, quiescent airway basal stem cells are derived from proliferative primordial progenitors through the cell-cycle slowdown. In contrast, basal cells contribute to adult tissue regeneration by shifting from slow cycling to proliferating and subsequently back to slow cycling. Although sustained proliferation results in tumorigenesis, the molecular mechanisms regulating these transitions remain unknown. Using temporal single-cell transcriptomics of developing murine airway progenitors and genetic validation experiments, we found that TGF-β signaling decelerated cell cycle by inhibiting Id2 and contributed to slow-cycling basal cell specification during development. In adult tissue regeneration, reduced TGF-β signaling restored Id2 expression and initiated regeneration. Id2 overexpression and Tgfbr2 knockout enhanced epithelial proliferation; however, persistent Id2 expression drove basal cell hyperplasia that resembled a precancerous state. Together, the TGF-β-Id2 axis commonly regulates the proliferation transitions in basal cells during development and regeneration, and its fine-tuning is critical for normal regeneration while avoiding basal cell hyperplasia.
Hirofumi Kiyokawa, Akira Yamaoka, Chisa Matsuoka, Tomoko Tokuhara, Takaya Abe, Mitsuru Morimoto

2160 related Products with: Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration.

1 x 10^6 cells/vial3 Modulators0.1ml (1mg/ml)100 extractions

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#34129261   2021/06/15 To Up

Influence of Iron Sulfide Nanoparticle Sizes in Solid-State Batteries.

Given the inherent performance limitations of intercalation-based lithium-ion batteries, solid-state conversion batteries are promising systems for future energy storage. A high specific capacity and natural abundancy make iron disulfide (FeS 2 ) a promising cathode active material. In this work, FeS 2  nanoparticles were prepared solvothermally. By adjusting the synthesis conditions, samples with average particle diameters between 10 nm and 35 nm were synthesized. The electrochemical performance was evaluated in solid-state cells with a Li-argyrodite solid electrolyte. While the reduction of FeS 2  was found to be irreversible in the initial discharge, a stable cycling of the reduced species was observed subsequently. A positive effect of smaller particle dimensions on FeS 2  utilization was identified, which can be attributed to a higher interfacial contact area and shortened diffusion pathways inside the FeS 2  particles. These results highlight the general importance of morphological design to exploit the promising theoretical capacity of conversion electrodes in solid-state batteries.
Georg F Dewald, Zainab Liaqat, Martin Alexander Lange, Wolfgang Tremel, Wolfgang Zeier

1549 related Products with: Influence of Iron Sulfide Nanoparticle Sizes in Solid-State Batteries.

5 mg1 mg1 mg1 mg100 mg1 mg5 mg2 mg25 mg5 mg5 mg1 mg

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#34129186   2021/06/15 To Up

Role of thyroid hormone therapy in depressive disorders.

The close association among thyroid metabolism, mood disorders and behavior has long been known. The old and modern uses of thyroid hormones to modulate the expression of depression and bipolar disorder and to improve clinical outcome when used in conjunction with psychotropic medications.
M Bauer, P C Whybrow

1218 related Products with: Role of thyroid hormone therapy in depressive disorders.

100 UG1 kit(96 Wells)96 tests100 96T96 assays5 x 50 ug100 96T

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#34128686   2021/06/15 To Up

Engineering Two-Dimensional Metal-Organic Framework on Molecular Basis for Fast Li Conduction.

Metal-organic frameworks (MOFs) have been proposed as emerging fillers for composite polymer electrolytes (CPEs). However, MOF particles are usually served as passive fillers that yield limited ionic conductivity improvement. Building continuous MOF reinforcements and exploiting their active roles remain challenging. Here we demonstrate the feasibility of engineering fast Li conduction within MOF on molecule conception. Two-dimensional Cu(BDC) MOF is selected as an active filler due to its sufficient accessible open metal sites for perchlorate anion anchoring to release free Li, verified by theoretical calculations and measurements. A novel Cu(BDC)-scaffold-reinforced CPE is developed growth of MOF, which provides fast Li channels inside MOF and continuous Li paths along the MOF/polymer interface for high Li conductivity (ambient 0.24 mS cm) and enables high mechanical strength. Stable cycling is achieved in solid-state Li-NCM811 full cell using the MOF-reinforced CPE. This molecule-basis Li conduction strategy brings new ideas for designing advanced CPEs.
Jianming Yu, Taolian Guo, Chao Wang, Zihan Shen, Xunyi Dong, Shiheng Li, Huigang Zhang, Zhenda Lu

2220 related Products with: Engineering Two-Dimensional Metal-Organic Framework on Molecular Basis for Fast Li Conduction.

50 ug10 mg10 ug20 ug1 LITRE 100ul25 mg50 ligations

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#34128548   2021/06/15 To Up

The impact of invertebrate decomposers on plants and soil.

Soil invertebrates make significant contributions to the recycling of dead plant material across the globe. However, studies focussed on the consequences of decomposition for plant communities largely ignore soil fauna across all ecosystems, because microbes are often considered the primary agents of decay. Here, we explore the role of invertebrates as not simply facilitators of microbial decomposition, but as true decomposers, able to break down dead organic matter with their own endogenic enzymes, with direct and indirect impacts on the soil environment and plants. We recommend a holistic view of decomposition, highlighting how invertebrates and microbes act in synergy to degrade organic matter, providing ecological services that underpin plant growth and survival.
Hannah M Griffiths, Louise A Ashton, Catherine L Parr, Paul Eggleton

2161 related Products with: The impact of invertebrate decomposers on plants and soil.

25 ml Ready-to-use 25 mg500 Units2 1000 tests100ug2.5 mg200ul1100ul 6 ml

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