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Search results for: Cytometry

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#33096430   2020/10/14 To Up

One-step quantification of salivary exosomes based on combined aptamer recognition and quantum dot signal amplification.

As promising fluid biomarkers for non-invasive diagnosis, naturally-occurring exosomes in saliva have attracted a wide interest for their potential application in oral diseases especially oral cancers. However, accurate quantification of salivary exosomes is still challenging due to the current difficulties in simultaneous identification and measurement of these nano-sized vesicles. In this study, we developed a novel fluorescent biosensor for one-step sensitive quantification of salivary exosomes based on magnetic and fluorescent bio-probes (MFBPs). Within the MFBPs, self-assembled DNA concatamers loaded with numerous quantum dots (QDs) were ingeniously tethered to aptamers, which were anchored on the surface of magnetic microspheres (MMs). Efficient recognition and capture of an exosome by the aptamer would simultaneously trigger the release of a DNA concatamer as the detection signal carrier, thereby generating a "one exosome-numerous QDs" amplification effect. As the result, this biosensor allowed one-step quantification with less assay time and achieved a high sensitivity with low limit of detection. Moreover, unique fluorescent properties of QDs and the superparamagnetism of MMs offered a strong anti-interference ability, enabling a robust quantification in complex matrices. Furthermore, this biosensor exhibited a good clinical feasibility with favorable accuracy comparable to nanoscale flow cytometry, and a superiority in label-free analysis and convenient operation. This study provides a novel and general strategy for one-step sensitive quantification of exosomes from body fluids, facilitating the development of exosome-based liquid biopsy for disease diagnosis.
Min Wu, Zhuokun Chen, Qihui Xie, Bolin Xiao, Gang Zhou, Gang Chen, Zhuan Bian

2332 related Products with: One-step quantification of salivary exosomes based on combined aptamer recognition and quantum dot signal amplification.

100 mg 500 ml 25 ml 1 ml 25 MG100ul5 mL1 g

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#33096404   2020/10/07 To Up

Oral commensal bacteria differentially modulate epithelial cell death.

Epithelial cell death is an important innate mechanism at mucosal surfaces, which enables the elimination of pathogens and modulates immunoinflammatory responses. Based on the antimicrobial and anti-inflammatory properties of cell death, we hypothesized that oral epithelial cell (OECs) death is differentially modulated by oral bacteria.
Tyresia White, Yelena Alimova, Vanessa Tubero Euzebio Alves, Pinar Emecen-Huja, Mohanad Al-Sabbagh, Alejandro Villasante, Jeffrey L Ebersole, Octavio A Gonzalez

2458 related Products with: Oral commensal bacteria differentially modulate epithelial cell death.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized25 TESTS100ug Lyophilized100ug Lyophilized

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#33096314   2020/09/25 To Up

SP1 activated-lncRNA SNHG1 mediates the development of epilepsy via miR-154-5p/TLR5 axis.

Epilepsy is a one of the most frequent serious neurological disorders characterized by enduring and unprovoked seizures. The treatments to epilepsy are very limited and many patients are even resistant to current medications due to the elusive pathogenesis. Here, we sought to investigate the functions of lncRNA SNHG1 and miR-154-5p in epilepsy.
Meng-Wen Zhao, Wen-Jie Qiu, Pu Yang

1460 related Products with: SP1 activated-lncRNA SNHG1 mediates the development of epilepsy via miR-154-5p/TLR5 axis.

100 ug/vial100ug/vial100ìl x 10 vials5 ml1 mg1mg1 x 10^6 cells/vial500 µl100 ug/vial

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#33096269   2020/10/20 To Up

Neoadjuvant Chemotherapy Increases Cytotoxic T cell, Tissue Resident Memory T cell and B Cell Infiltration in Resectable Non-Small Cell Lung Cancer.

The combination of PD-1/PD-L1 immune checkpoint blockade (ICB) and chemotherapy has revolutionized the treatment of advanced non-small cell lung cancer (NSCLC), but the mechanisms underlying this synergy remain incompletely understood. In this study, we explored the relationships between neoadjuvant chemotherapy and the immune microenvironment (IME) of resectable non-small cell lung cancer (NSCLC) in order to identify novel mechanisms by which chemotherapy may enhance the effect of ICB.
Pierre-Olivier Gaudreau, Marcelo V Negrao, Kyle G Mitchell, Alexandre Reuben, Erin M Corsini, Jun Li, Tatiana Karpinets, Qi Wang, Lixia Diao, Jing Wang, Lorenzo Federico, Edwin R Parra-Cuentas, Roohussaba Khairullah, Carmen Behrens, Arlene M Correa, Daniel Gomez, Latasha Little, Curtis Gumbs, Humam Kadara, Junya Fujimoto, Daniel J McGrail, Ara A Vaporciyan, Steven G Swisher, Garrett Walsh, Mara B Antonoff, Annikka Weissferdt, Hai Tran, Emily Roarty, Cara Haymaker, Chantale Bernatchez, Jianhua Zhang, P Andrew Futreal, Ignacio I Wistuba, Tina Cascone, John V Heymach, Boris Sepesi, Jianjun Zhang, Don L Gibbons

1014 related Products with: Neoadjuvant Chemotherapy Increases Cytotoxic T cell, Tissue Resident Memory T cell and B Cell Infiltration in Resectable Non-Small Cell Lung Cancer.



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#33096245   2020/10/20 To Up

Variation in tumor pH affects pH-triggered delivery of peptide-modified magnetic nanoparticles.

Acidification of the extracellular matrix, an intrinsic characteristic of many solid tumors, is widely exploited for physiologically triggered delivery of contrast agents, drugs, and nanoparticles to tumor. However, pH of tumor microenvironment shows intra- and inter-tumor variation. Herein, we investigate the impact of this variation on pH-triggered delivery of magnetic nanoparticles (MNPs) modified with pH-(low)-insertion peptide (pHLIP). Fluorescent flow cytometry, laser confocal scanning microscopy and transmission electron microscopy data proved that pHLIP-conjugated MNPs interacted with 4 T1 cells in two-dimensional culture and in spheroids more effectively at pH6.4 than at pH7.2, and entered the cell via clathrin-independent endocytosis. The accumulation efficiency of pHLIP-conjugated MNPs in 4 T1 tumors after their intravenous injection, monitored in vivo by magnetic resonance imaging, showed variation. Analysis of the tumor pH profiles recorded with implementation of original nanoprobe pH sensor, revealed obvious correlation between pH measured in the tumor with the amount of accumulated MNPs.
Alexandra G Pershina, Olga Ya Brikunova, Alexander M Demin, Maxim A Abakumov, Alexander N Vaneev, Victor A Naumenko, Alexander S Erofeev, Peter V Gorelkin, Timur R Nizamov, Albert R Muslimov, Alexander S Timin, Dina Malkeyeva, Elena Kiseleva, Sergey V Vtorushin, Irina V Larionova, Elena A Gereng, Artem S Minin, Aidar M Murzakaev, Victor P Krasnov, Alexander G Majouga, Ludmila M Ogorodova

2619 related Products with: Variation in tumor pH affects pH-triggered delivery of peptide-modified magnetic nanoparticles.

2 Pieces/Box480/kit100ug Lyophilized1 ml2 Pieces/Box100 μg

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#33095946   2020/10/23 To Up

Collagen and soy peptides attenuate contractile loss from UVA damage and enhance the antioxidant capacity of dermal fibroblasts.

Wrinkles and extracellular matrix (ECM) loss are common signs of skin aging and are thought to be the result of damage caused by reactive oxygen species (ROS); ROS induces an imbalance between ECM degradation and production.
Sophia Yi Zhang, Molly Hood, Iris Xue Zhang, Clark Liang Chen, Lynn Lu Zhang, Jun Du

1506 related Products with: Collagen and soy peptides attenuate contractile loss from UVA damage and enhance the antioxidant capacity of dermal fibroblasts.

96100 mg50 ug 10 mg100.00 ul10 mg1 mg1,000 tests200ug100ul25 mg

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#33095875   // To Up

ICOS is widely expressed in cutaneous T-cell lymphoma, and its targeting promotes potent killing of malignant cells.

The treatment of advanced-stage cutaneous T-cell lymphoma (CTCL) remains an unmet medical need. Mogamulizumab, anti-KIR3DL2, and brentuximab vedotin (BV), an anti-CD30 antibody-drug conjugate (ADC) coupled with monomethyl-auristatin-E (MMAE), provided encouraging results, but new targeted therapies are needed. Inducible T-cell costimulator (ICOS), a T-cell costimulatory receptor, is a promising therapeutic target, not only because it is expressed by malignant T cells in CTCL but also because of its connection with the suppressive activity of regulatory T (Treg) cells. Immunohistochemical analysis revealed that ICOS was widely expressed by malignant cells in skin biopsy specimens from 52 patients with mycosis fungoides and Sézary syndrome (SS), as well as in involved node biopsy specimens from patients with SS. Furthermore, flow cytometry demonstrated its strong expression by circulating tumor cells in all our patients with SS. Percentages of ICOS+ Treg cells were significantly higher in patients with SS than in healthy donors. We then investigated the preclinical efficacy of anti-ICOS ADCs generated by coupling murine anti-ICOS monoclonal antibodies with MMAE and pyrrolobenzodiazepine. In 3 CTCL cell lines (Myla, MJ, and HUT78), we observed a significant dose-dependent decrease in cell viability in the presence of anti-ICOS ADCs. In addition, anti-ICOS-MMAE ADCs had an in vitro and in vivo efficacy superior to BV in a mouse xenograft model (MyLa). Finally, we assessed the efficacy of anti-ICOS ADCs in ICOS+ patient-derived xenografts from patients with SS and angioimmunoblastic T-cell lymphoma. Collectively, our findings provide the preliminary basis for a therapeutic trial.
Florent Amatore, Nicolas Ortonne, Marc Lopez, Florence Orlanducci, Rémy Castellano, Saskia Ingen-Housz-Oro, Amandine De Croos, Clémentine Salvado, Laurent Gorvel, Armelle Goubard, Yves Collette, Réda Bouabdallah, Jean-Marc Schiano, Nathalie Bonnet, Jean-Jacques Grob, Philippe Gaulard, Martine Bagot, Armand Bensussan, Philippe Berbis, Daniel Olive

1846 related Products with: ICOS is widely expressed in cutaneous T-cell lymphoma, and its targeting promotes potent killing of malignant cells.

1x10e7 cells100ug Lyophilized100.00 ug100ug Lyophilized100ug Lyophilized100ug Lyophilized96 tests1x10e7 cells96T

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#33095809   2020/10/23 To Up

Enumerating regulatory T cells in cryopreserved umbilical cord blood samples using FOXP3 methylation specific quantitative PCR.

Allogeneic haematopoietic cell transplantation (HCT) is a curative therapy for severe haematological disorders. However, it carries significant risk of morbidity and mortality. To improve patient outcomes, better graft selection strategies are needed, incorporating HLA matching with clinically important graft characteristics. Studies have shown that the cellular content of HCT grafts, specifically higher ratios of T regulatory (Tregs)/T cells, are important factors influencing outcomes when using adult peripheral blood mobilised grafts. So far, no equivalent study exists in umbilical cord blood (CB) transplantation due to the limitations of cryopreserved CB samples.
Richard C Duggleby, Hoi Pat Tsang, Kathryn Strange, Alasdair McWhinnie, Abigail A Lamikanra, David J Roberts, Diana Hernandez, J Alejandro Madrigal, Robert D Danby

2631 related Products with: Enumerating regulatory T cells in cryopreserved umbilical cord blood samples using FOXP3 methylation specific quantitative PCR.

100 extractions96 tests100ml100 extractions2 Pieces/Box16 Arrays/Slide96 tests

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#33095510   2020/10/23 To Up

Acute monocytic leukemia presenting as generalized lymphadenopathy and skin rash in a toddler: highlighting the clinicopathologic mimics.

Acute myeloid leukemia (AML) is the fifth most common malignancy in children. Extramedullary involvement in acute myeloid leukemia is rare and can be seen in soft tissues, central nervous system, skin and lymphoreticular organs. The clinical presentations can often be non-specific and hence, the diagnosis can be very challenging, especially in cases without a prior hematologic diagnosis. We report a case of pediatric acute monocytic leukemia presenting with generalized lymphadenopathy and cutaneous rash. Fine-needle aspiration was performed from the lymph nodes and a cytologic diagnosis of infiltration by a lymphoreticular malignancy was suggested. Peripheral blood, bone marrow and cerebrospinal fluid involvement were noted subsequently. Flow cytometry on the bone marrow aspirate confirmed a diagnosis of acute monocytic leukemia. The index case besides highlighting an uncommon presentation of acute monocytic leukemia in a toddler, also emphasizes the need to consider acute monocytic leukemia as a cytomorphologic differential in such presentations.
Aleena Jain, Parikshaa Gupta, Nalini Gupta, Gnanamani Senguttuvan, Alpeshkumar Bipinbhai Kapadia, Balamurugan Thirunavukkarasu, Ritambhra Nada, Neelam Varma

1212 related Products with: Acute monocytic leukemia presenting as generalized lymphadenopathy and skin rash in a toddler: highlighting the clinicopathologic mimics.

100 μg48 assays 100 μg100 assays20 ul (10 mM)96 samples1 kit

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#33095438   2020/10/23 To Up

Circular RNA Circ_ANKMY2 Regulates Temporal Lobe Epilepsy Progression via the miR-106b-5p/FOXP1 Axis.

Temporal lobe epilepsy (TLE) is common intractable epilepsy that affects the patient's lives. The circular RNA circ_ANKMY2 (circ_ANKMY2) has been reported to be abnormally expressed in TLE. Nevertheless, the role and mechanism of circ_ANKMY2 in TLE are unclear. A human neuroblastoma cell line (SK-N-AS) was used for a series of studies. Expression levels of circ_ANKMY2, miR-106b-5p, and Forkhead Box Protein 1 (FOXP1) mRNA in TLE tissues were assessed through quantitative real-time polymerase chain reaction (qRT-PCR). Cell colony formation, proliferation, and apoptosis were determined by cell colony formation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), or flow cytometry assays. The levels of FOXP1 protein, Ki67, B cell lymphoma (Bcl-2), Bcl-2 Associated X (Bax), and Cleaved caspase-3 were evaluated by western blot analysis. The relationship between circ_ANKMY2 or FOXP1 and miR-106b-5p was verified with dual-luciferase reporter assay. We observed that circ_ANKMY2 and FOXP1 expression were reduced while miR-106b-5p expression was increased in TLE tissues. Overexpression of circ_ANKMY2 decreased spontaneous recurrent seizures (SRSs) in rat TLE model and blocked cell colony formation, proliferation, and induced cell apoptosis in SK-N-AS cells. Importantly, circ_ANKMY2 was verified as a sponge for miR-106b-5p. In addition, miR-106b-5p mimics abolished circ_ANKMY2 elevation-mediated effects on colony formation, proliferation, and apoptosis of SK-N-AS cells. Also, FOXP1 served as a target for miR-106b-5p. And FOXP1 silencing overturned the effects of miR-106b-5p inhibitors on the colony formation, proliferation, and apoptosis of SK-N-AS cells. In sum, circ_ANKMY2 modulated TLE advancement via regulation of FOXP1 expression through sponging miR-106b-5p, and circ_ANKMY2 might be an underlying target for the improvement of TLE.
Qing Lin, Jinying Chen, Xian Zheng, Yi Zhang, Xiaoxiao Tao, Jiamei Ye

1329 related Products with: Circular RNA Circ_ANKMY2 Regulates Temporal Lobe Epilepsy Progression via the miR-106b-5p/FOXP1 Axis.

20μg/vial100 ug/vial250ul100.00 ul75мg/vial200ul

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