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#35764395   2022/06/28 To Up

The Pathophysiology of Sepsis-Associated AKI.

Sepsis-associated AKI is a life-threatening complication that is associated with high morbidity and mortality in patients who are critically ill. Although it is clear early supportive interventions in sepsis reduce mortality, it is less clear that they prevent or ameliorate sepsis-associated AKI. This is likely because specific mechanisms underlying AKI attributable to sepsis are not fully understood. Understanding these mechanisms will form the foundation for the development of strategies for early diagnosis and treatment of sepsis-associated AKI. Here, we summarize recent laboratory and clinical studies, focusing on critical factors in the pathophysiology of sepsis-associated AKI: microcirculatory dysfunction, inflammation, NOD-like receptor protein 3 inflammasome, microRNAs, extracellular vesicles, autophagy and efferocytosis, inflammatory reflex pathway, vitamin D, and metabolic reprogramming. Lastly, identifying these molecular targets and defining clinical subphenotypes will permit precision approaches in the prevention and treatment of sepsis-associated AKI.
Shuhei Kuwabara, Eibhlin Goggins, Mark D Okusa

2926 related Products with: The Pathophysiology of Sepsis-Associated AKI.

100 IU0.1 mg 5 G500 Units 100 G11250 IU100.00 ul

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#35764385   2022/06/28 To Up

Using Sterile Flocked Swabs as an Alternative Method for Rodent Health Monitoring.

Routine health monitoring is an integral part of managing SPF rodent colonies. In recent years, rack-level environmental sampling has been introduced as an adjunct method or replacement for exposure of sentinel rodents to soiled bedding. However, rack level environmental monitoring is not compatible with rodent housing systems that have cage-level filtration. The current study investigated whether exposure of sterile flocked swabs to soiled bedding can be an alternative sampling method for routine health monitoring in mice, thus replacing the use of sentinels in soiled bedding cages. Flocked swabs were placed in cages containing pooled samples of soiled bedding but no mice; swabs remained there for 90 d, with weekly agitation and biweekly swabbing of the cage floor to mimic the agitation of soiled bedding by sentinel mice and facilitate the collection of dust particles. Fecal samples were collected from both colony and sentinel mice. For environmental samples, exhaust debris was collected from the rack plenum, and dust samples were collected from the exhaust hose. All samples were collected on days 88 through 91 and were tested for multiple pathogens by using real-time PCR assays. To determine the diagnostic agreement of flocked swab sampling with the other methods, we used κ statistics to compare the test results from flocked swabs with those from sentinel feces, exhaust debris, and colony animal feces; we found excellent agreement between the colony feces and the flocked swab methods. The sterile flocked swab method detected all enzootic pathogens in the colonies tested. Results from flocked swab samples had the least agreement with sentinel feces, which also failed to detect the presence of fur mites. This study supports the use of sterile flocked swabs as alternative to using sentinel mice, thus conforming to the guiding principles of replacement and reduction in the use of animals for routine colony health monitoring.
Mynn Michele D Varela, Jan Irving A Bibay, Bryan E Ogden, Marcus J Crim, Hla M Htoon

1627 related Products with: Using Sterile Flocked Swabs as an Alternative Method for Rodent Health Monitoring.

96 Tests50 assays100Tests100μl100ug Lyophilized 100ul100 ug100ug25 assays

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#35764366   // To Up

Development of anti-somatostatin receptors CAR T cells for treatment of neuroendocrine tumors.

Neuroendocrine tumors (NETs) overexpress somatostatin receptors (SSTRs).
Barbara Mandriani, Eleonora Pellè, Francesco Mannavola, Antonio Palazzo, Renè Massimiliano Marsano, Giuseppe Ingravallo, Gerardo Cazzato, Maria Cecilia Ramello, Camillo Porta, Jonathan Strosberg, Daniel Abate-Daga, Mauro Cives

2958 related Products with: Development of anti-somatostatin receptors CAR T cells for treatment of neuroendocrine tumors.

100μg1 mg1 mg100ug Lyophilized100ul0.1ml (1mg/ml)100μg100ug Lyophilized

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#35764345   2022/06/28 To Up

Impact of Changes in Early Respiratory Support Management on Respiratory Outcomes of Preterm Infants.

In the period immediately after birth, preterm infants are highly susceptible to lung injury. Ventilator-induced lung injury has been recognized as a major contributing factor for bronchopulmonary dysplasia (BPD) in preterm infants. Noninvasive respiratory support (NIRS) could decrease lung injury, and early respiratory support management might affect pulmonary outcomes. We conducted a study to evaluate the changes in early respiratory support management and their impact on respiratory outcome and complications of preterm infants in 3 different time periods over the last 13 years.
Lulu Xie, Xianqiong Luo, Bing Li, Lanlan Du, Zhu Wang, Ying Liu, Jia Chen, Shunyan Duan, Longli Yan, Weiwei Gao

1711 related Products with: Impact of Changes in Early Respiratory Support Management on Respiratory Outcomes of Preterm Infants.

5 G100 μg100ug96T96T100ug Lyophilized2 mg100100 assays5µg

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#35764337   2022/06/28 To Up

Anti-NMDA receptor encephalitis with initial negative markers: diagnostic and therapeutic challenges of a refractory case with 9-month-long follow-up.

Anti--methyl-d-aspartate (NMDA) receptor encephalitis is a progressively debilitating, frequently fatal disease caused by autoantibodies against the NMDA receptor. Risk of delayed treatment is high due to variable presentations, lack of awareness and potential false negative diagnostic studies. In this case report, a woman in her 20s presented with psychiatric manifestations and rapidly declined. Dyskinetic movements and dysautonomia were observed. Initial cerebrospinal fluid and serum anti-NMDA receptor antibodies were negative. MRI was inconclusive. Electroencephalography demonstrated extreme delta brush. Pelvic CT revealed an adnexal teratoma. She remained refractory to treatment until day 126 when, after two cycles of cyclophosphamide, she started to improve. She participated in rehabilitation with eventual discharge home on day 269. Recognising the variable presentations of anti-NMDA receptor encephalitis is important in avoiding misdiagnosis and delayed treatment. If clinical suspicion remains high despite negative results, repeat testing should be pursued. Clinical response should guide treatment decisions in refractory cases.
Camilla Cascardo, Alexander Ismail, Joseph Fullmer, Francisco Davila

1179 related Products with: Anti-NMDA receptor encephalitis with initial negative markers: diagnostic and therapeutic challenges of a refractory case with 9-month-long follow-up.

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#35764236   2022/06/25 To Up

Brief Report: Exploratory analysis of maintenance therapy in patients with extensive-stage small cell lung cancer treated first-line with atezolizumab plus carboplatin and etoposide.

In the Phase I/III IMpower133 study, atezolizumab plus carboplatin and etoposide (CP/ET) followed by maintenance atezolizumab for first-line treatment of extensive-stage small-cell lung cancer (ES-SCLC) led to significant improvement in both overall survival (OS) and progression-free survival (PFS) versus placebo plus CP/ET followed by maintenance placebo. We explored the benefit of atezolizumab versus placebo in the subset of patients who reached the IMpower133 maintenance phase as well as the safety profile of maintenance therapy.
Martin Reck, Tony S K Mok, Aaron Mansfield, Richard De Boer, Gyorgy Losonczy, Shunichi Sugawara, Rafal Dziadziuszko, Maciej Krzakowski, Alexey Smolin, Maximilian Hochmair, Marina Chiara Garassino, Gilberto de Castro Junior, Helge Bischoff, Sivuonthanh Lam, Andres Cardona, Stefanie Morris, Stephen V Liu

2783 related Products with: Brief Report: Exploratory analysis of maintenance therapy in patients with extensive-stage small cell lung cancer treated first-line with atezolizumab plus carboplatin and etoposide.



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#35764205   2022/06/25 To Up

Effectiveness of Vitamin D Supplementation on Knee Osteoarthritis - A target trial emulation study using data from the Osteoarthritis Initiative cohort.

To assess the real-world effectiveness of vitamin D supplementation in patients with knee osteoarthritis (KOA) by replicating a randomized controlled trial (RCT) design in an observational study.
Xingzhong Jin, Changhai Ding, David J Hunter, Blanca Gallego

1814 related Products with: Effectiveness of Vitamin D Supplementation on Knee Osteoarthritis - A target trial emulation study using data from the Osteoarthritis Initiative cohort.

0.1ml (1mg/ml)0.1ml (1mg/ml)

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#35764196   2022/06/25 To Up

Anticancer activity of D-LAK-120A, an antimicrobial peptide, in non-small cell lung cancer (NSCLC).

Non-small cell lung cancer (NSCLC) is a major cause of global cancer mortalities and accounts for approximately 80-85% of reported lung cancer cases. Conventional chemotherapeutics show limited application because of poor tumor selectivity and acquired drug resistance. Antimicrobial peptides (AMPs) have gained much attention as potential anticancer therapeutics owing to their high potency and high target selectivity and specificity with limited scope for drug resistance. In this study, D-LAK (D-LAK-120A), a cationic AMP, was evaluated for its anticancer efficacy in various NSCLC cell lines. D-LAK peptide demonstrated enhanced cytotoxicity in A549, H358, H1975, and HCC827 cell lines with inhibitory concentrations between 4.0 and 5.5 μM. An increase in the lactate dehydrogenase (LDH) levels and propidium iodide (PI) uptake across compromised membrane suggested the membranolytic activity as an inhibition pathway. In addition, we found D-LAK induced lung cancer cell apoptosis and arrested cells in the S phase (DNA synthesis) of cell cycle. Moreover, a decreased mitochondrial membrane potential and elevated ROS levels were observed after D-LAK treatment, suggesting induction of mitochondria-mediated apoptosis. Additionally, D-LAK inhibited single cell proliferation and cancer cell migration in vitro. The tumor reduction observed in the 3D spheroid assay further suggests the potential use of D-LAK as an anticancer agent for NSCLC treatment. Our results postulate innovative insights on the anticancer mechanism of D-LAK, which may contribute to its further development into preclinical studies and a potential therapeutic.
Suyash M Patil, Nitesh K Kunda

1399 related Products with: Anticancer activity of D-LAK-120A, an antimicrobial peptide, in non-small cell lung cancer (NSCLC).



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#35764171   2022/06/25 To Up

PvdM of fluorescent pseudomonads is required for the oxidation of ferribactin by PvdP in periplasmic pyoverdine maturation.

Fluorescent pseudomonads such as Pseudomonas aeruginosa or Pseudomonas fluorescens produce pyoverdine siderophores that ensure iron-supply in iron-limited environments. After its synthesis in the cytoplasm, the non-fluorescent pyoverdine precursor ferribactin is exported into the periplasm, where the enzymes PvdQ, PvdP, PvdO, PvdN, and PtaA are responsible for fluorophore maturation and tailoring steps. While the roles of all these enzymes are clear, little is known about the role of PvdM, a human renal dipeptidase-related protein that is predicted to be periplasmic and that is essential for pyoverdine biogenesis. Here we reveal the subcellular localization and functional role of PvdM. Using the model organism P. fluorescens, we show that PvdM is anchored to the periplasmic side of the cytoplasmic membrane, where it is indispensable for the activity of the tyrosinase PvdP. While PvdM does not share the metallopeptidase function of renal dipeptidase, it still has the corresponding peptide-binding site. The substrate of PvdP, deacylated ferribactin, is secreted by a ΔpvdM mutant strain, indicating that PvdM prevents loss of this periplasmic biosynthesis intermediate into the medium by ensuring the efficient transfer of ferribactin to PvdP in vivo. We propose that PvdM belongs to a new dipeptidase-related protein subfamily with inactivated Zn coordination sites, members of which are usually genetically linked to TonB-dependent uptake systems and often associated with periplasmic FAD-dependent oxidoreductases related to D-amino acid oxidases. We suggest that these proteins are necessary for selective binding, exposure, or transfer of specific D- and L-amino acid-containing peptides and other periplasmic biomolecules in manifold pathways.
Michael-Frederick Sugue, Ali Nazmi Burdur, Michael T Ringel, Gerald Dräger, Thomas Brüser

2331 related Products with: PvdM of fluorescent pseudomonads is required for the oxidation of ferribactin by PvdP in periplasmic pyoverdine maturation.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized1 ml100 μg100ug Lyophilized100ug Lyophilized50ul (1mg/ml)100 ul

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