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Search results for: DISTEMPER

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#33078460   2020/10/20 To Up

Crystal structure of the giant panda MHC class I complex: first insights into the viral peptide presentation profile in the bear family.

The viral cytotoxic T lymphocyte (CTL) epitope peptides presented by classical MHC-I molecules require the assembly of a peptide-MHC-I-β2m (pMHC-I) trimolecular complex for T cell receptor (TCR) recognition, which is the critical activation link for triggering antiviral T cell immunity. Research on T cell immunology in the Ursidae family, especially structural immunology, is still lacking. In this study, the structure of the key trimolecular complex pMHC-I, which binds a peptide from canine distemper virus, was solved for the first time using giant panda as a representative species of Ursidae. The structural characteristics of the giant panda pMHC-I complex (pAime-128), including the unique pockets in the peptide-binding groove (PBG), were analyzed in detail. Comparing the pAime-128 to others in the bear family and extending the comparison to other mammals revealed distinct features. The interaction between MHC-I and β2m, the features of pAime-128 involved in TCR docking and cluster of differentiation 8 (CD8) binding, the anchor sites in the PBG, and the CTL epitopes of potential viruses that infect pandas were clarified. Unique features of pMHC-I viral antigen presentation in the panda were revealed by solving the three-dimensional (3D) structure of pAime-128. The distinct characteristics of pAime-128 indicate an unusual event that emerged during the evolution of the MHC system in the bear family. These results provide a new platform for research on panda CTL immunity and the design of vaccines for application in the bear family. This article is protected by copyright. All rights reserved.
Hongyu Yuan, Lizhen Ma, Lijie Zhang, Xiaoying Li, Chun Xia

1630 related Products with: Crystal structure of the giant panda MHC class I complex: first insights into the viral peptide presentation profile in the bear family.

150 5 mg1

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#33065872   // To Up

L'étude de la maladie de Carré chez ses hôtes naturels : un modèle de pathogenèse morbillivirale.

Morbilliviruses cause a severe and sometimes lethal disease in their respective hosts, which is characterized by a generalized immunosuppression, respiratory and gastro-intestinal clinical signs, and occasional neurological complications. This similarity in the biology of different members of the morbillivirus genus constitutes the basis for the study of canine distemper virus in its natural hosts as a model for the characterization of morbillivirus pathogenesis. In combination with the reverse genetics technology, which allows the production of recombinant viruses carrying specific genetic modifications, this model has made important contributions to our understanding of the mechanisms underlying morbillivirus immunosuppression, dissemination, and neuroinvasion.
Sébastien Delpeut, Ronan Nicolas Rouxel, Veronika von Messling

1783 related Products with: L'étude de la maladie de Carré chez ses hôtes naturels : un modèle de pathogenèse morbillivirale.

1kit30 mg100Tests96 tests10 mg100tests3 mg300 units25 mg2.5 mg

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#33028721   2020/10/07 To Up

Clustered Lysine Residues of the Canine Distemper Virus Matrix Protein Regulate Membrane Association and Budding Activity.

The canine distemper virus (CDV) matrix (M) protein is multifunctional; it orchestrates viral assembly and budding, drives the formation of virus-like particles (VLPs), regulates viral RNA synthesis and may support additional functions. CDV M may assemble into dimers, where each protomer is constituted by N-terminal and C-terminal domains (NTD and CTD, respectively). Here, to investigate whether electrostatic interactions between CDV M and the plasma membrane (PM) may contribute to budding activity, selected surface-exposed positively charged lysine residue, which locate within a large basic patch of CTD, were replaced by amino acids with selected properties. We found that some M-mutants harboring amino acid with neutral and positive charge (methionine and arginine, respectively) maintained full functionality, including proper interaction and localization with the PM as well as intact VLP and progeny virus production, as demonstrated by employing a cell exit-complementation system. Conversely, while the overall structural integrity remained mostly unaltered, most of the nonconservative M-variants (carrying a glutamic acid; negatively charged) exhibited a cytosolic phenotype secondary to the lack of interaction with the PM. Consequently, such M-variants were entirely defective in VLP production and viral particle formation. Furthermore, the proteasome inhibitor Bortezomib significantly reduced wild-type M-mediated VLP production. Nevertheless, in absence of the compound, all engineered M lysine-variants exhibited unaffected ubiquitination profiles, consistent with other residues likely involved in this functionally-essential post-translational modification. Altogether, our data identified multiple surface-exposed lysine residues, located within a basic patch of CDV M-CTD, critically contributing to PM association and ensuing membrane budding activity. Although vaccines against some morbilliviruses exist, infections still occur, which can result in dramatic brain disease or fatal outcome. Post-exposure prophylaxis with antivirals would support global vaccination campaigns. Unfortunately, there is no efficient antiviral drug currently approved. The matrix (M) protein of morbilliviruses coordinates viral assembly and egress through interaction with multiple cellular and viral components. However, molecular mechanisms supporting these functions remain poorly understood, which preclude the rationale design of inhibitors. Here, to investigate potential interactions between canine distemper virus (CDV) M and the plasma membrane (PM), we combined structure-guided mutagenesis of selected surface-exposed lysine residues with biochemical, cellular and virological assays. We identified several lysines clustering in a basic patch microdomain of the CDV M C-terminal domain, which contributed to PM association and budding activity. Our findings provides novel mechanistic information of how morbilliviruses assemble and egress from infected cells, thereby delivering bases for future antiviral drug development.
Nicole P Kadzioch, Matthieu Gast, Francesco Origgi, Philippe Plattet

1366 related Products with: Clustered Lysine Residues of the Canine Distemper Virus Matrix Protein Regulate Membrane Association and Budding Activity.

0.1 mg0.25 mg200 10000.25 mg0.25 mg5001001 mL0.1 mg1001 mg

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#33016015   // To Up

Isolation and molecular characterizations of canine distemper virus from a naturally infected Korean dog using Vero cells expressing dog signaling lymphocyte activation molecule.

Canine distemper virus (CDV) infection results in high morbidity and mortality in dogs. There has been no report about isolation of Korean CDV since 1980 in Korea.
Dong Kun Yang, Ha Hyun Kim, Siu Lee, Yoon Seek Yoon, Jungwon Park, Dongryul Oh, Jae Young Yoo, Miryeon Ji, Bokhee Han, Subin Oh, Bang Hun Hyun

2488 related Products with: Isolation and molecular characterizations of canine distemper virus from a naturally infected Korean dog using Vero cells expressing dog signaling lymphocyte activation molecule.

0.1 mg0.1 mg0.25 mg0.25 mg0.25 mg1.00 flask10100 µg2100 µg0.25 mg.

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#33012001   2020/10/03 To Up

Harnessing genomics to trace the path of a viral outbreak in African lions.

Predicting the emergence of novel infectious diseases requires an understanding of how pathogens infect and efficiently spread in alternative naïve hosts. A pathogen's ability to adapt to a new host (i.e. host shift) oftentimes is constrained by host phylogeny, due to limits in the molecular mechanisms available to overcome host-specific immune defenses (Longdon, Brockhurst, Russell, Welch, & Jiggins, 2014). Some pathogens, such as RNA viruses, however, have a propensity to jump hosts due to rapid mutation rates. For example, canine distemper virus (CDV) infects a broad range of terrestrial carnivores, as well as non-carnivore species worldwide, with a host range that is distributed across 5 orders and 22 families (Beineke, Baumgärtner, & Wohlsein, 2015). In 1993-1994, a severe CDV outbreak infected multiple carnivore host species in Serengeti National Park, causing widespread mortality and the subsequent decline of the African lion (Panthera leo) population (Roelke-Parker et al., 1996). While previous studies established domestic dogs (Canis lupus familiaris) as the disease reservoir, the precise route of transmission to lions remained a mystery, and a number of wild carnivore species could have facilitated viral evolution and spread. In this issue of Molecular Ecology, Weckworth et al. 2020 used whole genome viral sequences obtained from four carnivore species during the CDV outbreak, in combination with epidemiological data, to illuminate the pathway and evolutionary mechanisms leading to disease emergence in Serengeti lions.
Pauline L Kamath

2184 related Products with: Harnessing genomics to trace the path of a viral outbreak in African lions.



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#33006556   // To Up

[Vaccinovigilance: Reports of adverse reactions in the year 2019].

The registration of adverse events after the use of immunological veterinary medicinal products (IVMP) is the aim of the vigilance reporting system in Switzerland. Adverse events comprise suspected adverse reactions and lack of expected efficacy. Since the Institute of virology and immunology (IVI) is the competent authority for the regulation of immunological VMP in Switzerland, the reporting system is administrated by the IVI. In 2019, 137 reports concerning authorized immunological VMP were received (15% less compared to 2018). While most of the reports were submitted by the marketing authorization holders (56%), practicing veterinary surgeons contributed to the reporting system, too (40%). This corresponds to an increase of 22% of reported adverse events by the practicing veterinary surgeons compared to the previous year. Private persons (4%) submitted five reports. In comparison to 2018, in 2019 79% of the adverse events were reported by marketing authorization holders and 18% by veterinarians. Dogs (55%) and cats (20%) were mainly affected. Further reports were related to cattle (13%) and horses (5%). Recently, the numbers of reports concerning dogs (+12%) and cats (+4%) have considerably increased. Most of the reports were based on the application of vaccines against canine distemper, hepatitis, parvovirosis and parainfluenza in combination with leptospirosis in dogs as well as cat flu and feline panleukopenia in cats. In 34% of the submitted cases, the causality assessment between the vaccination and the reaction described was evaluated as probable.
I Zaugg, N Herrmann, H Ottiger

1552 related Products with: [Vaccinovigilance: Reports of adverse reactions in the year 2019].

1100ug Lyophilized2 Pieces/Box1 Set

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#32989769   2020/09/28 To Up

Presumed optic neuritis of non-infectious origin in dogs treated with immunosuppressive medication: 28 dogs (2000-2015).

To describe the clinical findings, magnetic resonance imaging features, management and outcome of canine cases with presumed optic neuritis of non-infectious origin that were presented to a UK referral centre from January 2000 to December 2015.
L Bedos, R Tetas, V Crespo, A Shea

1252 related Products with: Presumed optic neuritis of non-infectious origin in dogs treated with immunosuppressive medication: 28 dogs (2000-2015).

2000 Units500 tests50 ul500 tests50 ul10020 ul

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#32961282   2020/09/19 To Up

Codon usage bias in the H gene of canine distemper virus.

Canine distemper virus (CDV), a non-segmented single negative-stranded RNA (ssRNA), is the etiological agent of canine distemper. Canine distemper is a highly contagious and lethal viral disease in domestic dogs and wild carnivores. Study of the evolution of CDV presents an essential key to improve the vaccine efficacy. In this study, a total of 328 full-length CDV hemagglutinin (H) gene sequences were subjected to phylogenetic, amino acid mutations, and codon usage analysis. In accordance with previous study, CDV genotypes consisted of fifteen lineages. The unique amino acid substitution sites in each CDV lineages have been identified for the first time, including America-1 (Q330H), America-2 (I585S), Asia-1 (A359V), Asia-2 (H61R), Asia-3 (P108Q), Asia-4 (K213T), India-1/Asia-5(S497P), Arctic (S20L), Africa-1(N489S), Colombian (V41I), EWL (I44V), Europe (D560E), Europe-1/South America-1(K161Q), South America-2 (R580Q), and East African (S214A). Codon usage analysis indicated that H gene exhibited low codon usage bias and further neutrality plot analysis demonstrated that natural selection played a dominated role in driving CPV evolution. The effective number of codons (ENC) plots show that all the different sequences are below the standard curve, indicating that mutational pressure is not the only factor affecting CUB but other forces, including natural selection. The neutrality analysis showed that the slope of the regression line was 0.1501, indicating natural selection dominates directional mutation pressure in driving the codon usage pattern. In addition, nucleotide composition, relative synonymous codon usage value, dinucleotide content, and geographical distribution have been proven to influence the codon usage bias of the CDV H gene. The novel findings enhanced the understanding of CDV evolution.
Xin Wang, Weihua Xu, Kewei Fan, Hung-Chuan Chiu, Cuiqin Huang

1997 related Products with: Codon usage bias in the H gene of canine distemper virus.

0.25 mg5ug100 1 mL1 mg0.25 mg100 1 mL251010 2ug

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#32883344   2020/09/03 To Up

Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein.

Canine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2. The IgY single chain fragment variables (scFv) were generated by T7 phage display system and expressed in E. coli system. The titer of the primary scFv library reached to 1.5 × 10 pfu/mL, and 95% of the phages contained the target fragments. The CPV-VLP and CPV-VP2 protein showed similar reaction values to the purified scFv in the ELISA test, and the results of ELISA analysis using IgY-scFv toward CPV clinical samples were consistent with commercial immunochromatographic assay (ICA) and PCR detection, the scFv did not show cross reactivity with canine distemper virus (CDV) and canine coronavirus (CCV). IgY-scFv was successfully expressed in CRFK cells, and in the virus suppression assay, 55% of CPV infections were eliminated within 24 h. Docking results demonstrated that the number of amino acids of the binding sides between scFv and VP2 were AA37 and AA40, respectively. This study revealed the feasibility of a novel functional antibody fragment development strategy by generating diversified avian IgY-scFv libraries towards the pathogenic target of interest for both detection and therapeutic purposes in veterinary medicine.
Shikun Ge, Long Xu, Ben Li, Fagang Zhong, Xiang Liu, Xiaoying Zhang

1841 related Products with: Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein.

0.25 mg200 100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized0.1 mg100ug Lyophilized100ug Lyophilized100ug Lyophilized

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