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#33631800   // To Up

Nuclear DNA replicates during zygote development in Arabidopsis and Torenia fournieri.

The progression of the cell cycle is continuous in most cells, but gametes (sperm and egg cells) exhibit an arrest of the cell cycle to await fertilization to form a zygote, which then continues through the subsequent phases to complete cell division. The phase in which gametes of flowering plants arrest has been a matter of debate, since different phases have been reported for the gametes of different species. In this study, we reassessed the phase of cell-cycle arrest in the gametes of two species, Arabidopsis (Arabidopsis thaliana) and Torenia fournieri. We first showed that 4', 6-diamidino-2-phenylindole staining was not feasible to detect changes in gametic nuclear DNA in T. fournieri. Next, using 5-ethynyl-2'-deoxyuridine (EdU) staining that detects DNA replication by labeling the EdU absorbed by deoxyribonucleic acid, we found that the replication of nuclear DNA did not occur during gamete development but during zygote development, revealing that the gametes of these species have a haploid nuclear DNA content before fertilization. We thus propose that gametes in the G1 phase participate in the fertilization event in Arabidopsis and T. fournieri.
Xue-Qiong Liu, Jiao-Jiao Shi, Hua Fan, Jiao Jiao, Long Gao, Li Tan, Shingo Nagawa, Dan-Yang Wang

1571 related Products with: Nuclear DNA replicates during zygote development in Arabidopsis and Torenia fournieri.

2 Pieces/Box0.1ml (1mg/ml)300 units100 μg1 Set 100ul100 μg1 Set

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#33631700   2021/01/16 To Up

Transcriptome and epigenome analysis of porcine embryos from non-esterified fatty acid-exposed oocytes.

Increasing evidence indicates that maternal malnutrition leads to decreased female fertility and dysregulated metabolic homeostasis in offspring. High levels of non-esterified fatty acids (NEFAs) in follicular fluid were reported to be involved in these maternal nutritional effects, but the mechanisms remain unclear. This study explored the mechanisms of action of abnormal NEFA levels during porcine oocyte in vitro maturation (IVM) on early embryo development (blastocysts) using phenotypic, transcriptomic, and epigenetic analysis. The oocytes were treated during IVM with, in addition to the 1% (v/v) porcine follicular fluid in the control group, a combination of 468 μmol/L palmitic acid, 194 μmol/L stearic acid, and 534 μmol/L oleic acid supplemented to North Carolina State University-23 (NCSU-23) maturation medium to achieve a high level of NEFAs. After IVM, oocytes were in vitro fertilized and then cultured in regular conditions for blastocysts. Expanded blastocysts were collected to complete transcriptomic and epigenetic analysis. Macroscopically, high level of NEFAs impaired embryo development by reducing the blastocyst rate. Analysis of the transcriptome revealed that pathways related to inflammation, apoptosis, metabolism, and oxidative stress were the most affected. Moreover, DNA methylation data demonstrated differentially methylated regions in genes related to cellular metabolism and inflammation processes. Therefore, our conclusion is that high level of NEFAs during IVM might affect porcine early embryo development by diminishing blastocyst rate and altering gene expression, especially at the metabolism and cell status levels, which could further decrease the embryo quality.
M Shi, M-A Sirard

2693 related Products with: Transcriptome and epigenome analysis of porcine embryos from non-esterified fatty acid-exposed oocytes.

100g1KG100ug 100ul500gm10 mg100gm1 mg

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#33631698   2021/02/11 To Up

Multibiomarker-based assessment of toxicity of central European strains of filamentous cyanobacteria Aphanizomenon gracile and Raphidiopsis raciborskii to zebrafish Danio rerio.

The global increase in cyanobacterial blooms poses environmental and health threats. Selected cyanobacterial strains reveal toxicities despite a lack of synthesis of known toxic metabolites, and the mechanisms of these toxicities are not well understood. Here we investigated the toxicity of non-cylindrospermopsin and non-microcystin producing Aphanizomenon gracile and Raphidiopsis raciborskii of Central European origin to zebrafish exposed for 14 days to their extracts. Toxicological screening revealed the presence of anabaenopeptins and a lack of anatoxin-a, ß-methylamino-L-alanine or saxitoxins in examined extracts. The responses were compared to 20 μg L of common cyanobacterial toxins cylindrospermopsin (CYN) and microcystin-LR (MC-LR). The expression of the marker genes involved in apoptosis (caspase 3a and 3b, Bcl-2, BAX, p53, MAPK, Nrf2), DNA damage detection and repair (GADD45, RAD51, JUN, XPC), detoxification (CYP1A, CYP26, EPHX1), lipid metabolism (PPARa, FABP1, PLA2), phosphorylation/dephosphorylation (PPP6C, PPM1) and cytoskeleton (actin, tubulin) were examined using targeted transcriptomics. Cellular stress and toxicity biomarkers (oxidative injury, antioxidant enzymes, thiol pool status, and lactate dehydrogenase activity) were measured in the liver, and acetylcholinesterase activity was determined as an index of neurotoxicity in the brain. The extracts of three cyanobacterial strains that produce no known cyanotoxins caused marked toxicity in D. rerio, and the biomarker profiles indicate different toxic mechanisms between the bioactive compounds extracted from these strains and the purified cyanotoxins. All studied cyanobacterial extracts and purified cyanotoxins induced oxidative stress and neurotoxicity, downregulated Nrf2 and CYP26B1, disrupted phosphorylation/dephosphorylation processes and actin/tubulin cytoskeleton and upregulated apoptotic activity in the liver. The tested strains and purified toxins displayed distinctively different effects on lipid metabolism. Unlike CYN and MC-LR, the Central European strain of A. gracile and R. raciborskii did not reveal a genotoxic potential. These findings help to further understand the ecotoxicological consequences of toxic cyanobacterial blooms in freshwater ecosystems.
Halina Falfushynska, Oksana Horyn, Inna Osypenko, Piotr Rzymski, Łukasz Wejnerowski, Marcin K Dziuba, Inna M Sokolova

2995 related Products with: Multibiomarker-based assessment of toxicity of central European strains of filamentous cyanobacteria Aphanizomenon gracile and Raphidiopsis raciborskii to zebrafish Danio rerio.

5 G101 module10 mgOne 96-Well Microplate Ki1 module100ug 50 UG5 mg1 kit(96 Wells)1 ml

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#33631697   2021/02/09 To Up

Discovery of 4-alkoxy-2-aryl-6,7-dimethoxyquinolines as a new class of topoisomerase I inhibitors endowed with potent in vitro anticancer activity.

In our attempt to develop potential anticancer agents targeting Topoisomerase I (TOP1), two novel series of 4-alkoxy-2-arylquinolines 14a-p and 19a-c were designed and synthesized based on structure activity relationships of the reported TOP1 inhibitors and structural features required for stabilization of TOP1-DNA cleavage complexes (TOP1ccs). The in vitro anticancer activity of these two series of compounds was evaluated at one dose level using NCI-60 cancer cell lines panel. Compounds 14e-h and 14m-p, with p-substituted phenyl at C2 and propyl linker at C4, were the most potent and were selected for assay at five doses level in which they exhibited potent anticancer activity at sub-micromolar level against diverse cancer cell lines. Compound 14m was the most potent with full panel GI MG-MID 1.26 μM and the most sensitive cancers were colon cancer, leukemia and melanoma with GI MG-MID 0.875, 0.904 and 0.926 μM, respectively. Melanoma (LOX IMVI) was the most sensitive cell line to all tested compounds displaying GI from 0.116 to 0.227 μM, TGI from 0.275 to 0.592 μM and LC at sub-micromolar concentration against almost of the tested compounds. Compounds 14e-h and 14m-p were assayed using TOP1-mediated DNA cleavage assay to evaluate their ability to stabilize TOP1ccs resulting in cancer cell death. The morpholino analogs 14h and 14p exhibited moderate TOP1 inhibitory activity compared to 1 μM camptothecin suggesting their use as lead compounds that can be optimized for the development of more potent anticancer agents with potential TOP1 inhibitory activity. Finally, Swiss ADME online web tool predicted that compounds 14h and 14p possessed good oral bioavailability and druglikeness characteristics.
Mostafa M Elbadawi, Wagdy M Eldehna, Wenjie Wang, Keli K Agama, Yves Pommier, Manabu Abe

1892 related Products with: Discovery of 4-alkoxy-2-aryl-6,7-dimethoxyquinolines as a new class of topoisomerase I inhibitors endowed with potent in vitro anticancer activity.

400Tests1 kit48 assays 96T96 assays 96 tests48 assays

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#33631683   2021/02/17 To Up

Fish farm effluents as a source of antibiotic resistance gene dissemination on Jeju Island, South Korea.

The abuse or misuse of antibiotics is directly linked to the emergence of antibiotic-resistant bacteria and antibiotic resistance genes (ARGs) in the environment. Most fish farms located on Jeju Island operate a flow-through system that pumps in seawater for fish farming and discharges it back to the ocean. To investigate the amount of ARGs that these fish farm effluents discharge into the marine environment, we conducted a metagenomic-based resistome analysis. We observed higher levels of ARGs in fish farm effluents than in seawater at beach and residential areas. A greater proportion of ARGs was found on plasmid rather than on chromosomal DNA, especially for sulfonamide and phenicol classes. The distribution of ARGs did not differ between summer and winter, but the microbial community did. In addition, fish farm samples contained significantly more opportunistic pathogens (i.e., Vibrio, Photobacterium, Aliivibrio, and Tenacibaculum) and virulence factors than non-fish farm samples. Vibrio was the most frequently identified host of ARGs and virulence factors. The presence of Vibrio in the coastal area has been increasing owing to the recent rise in the temperature of seawater. This study suggests the need for actions to treat or monitor ARGs in the coastal areas where fish farms operating a flow-through system are located.
Hyejun Jo, Shahbaz Raza, Adeel Farooq, Jungman Kim, Tatsuya Unno

2524 related Products with: Fish farm effluents as a source of antibiotic resistance gene dissemination on Jeju Island, South Korea.

100ul10 Plates50ul 100ul50096 assays 100ul100 plates100 plates

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#33631672   2021/02/22 To Up

Structural basis of the p53 DNA binding domain and PUMA complex.

PUMA (p53-upregulated modulator of apoptosis) is localized in mitochondria and a direct target in p53-mediated apoptosis. p53 elicits mitochondrial apoptosis via transcription-dependent and independent mechanisms. p53 is known to induce apoptosis via the transcriptional induction of PUMA, which encodes proapoptotic BH3-only members of the Bcl-2 protein family. However, the transcription-independent mechanisms of human PUMA remain poorly defined. For example, it is not known whether PUMA interacts directly with the DNA binding domain (DBD: residues 92-293) of p53 in vitro. Here, the structure of the complex between the DBD of p53 and PUMA peptide was elucidated by X-ray crystallography. Isothermal titration calorimetry showed that PUMA peptide binds strongly with p53 DBD, and the crystal structure of p53-PUMA peptide complex revealed it contains four molecules of p53 DBD and one PUMA peptide per asymmetric unit in space group P. PUMA peptide bound to the N-terminal residues of p53 DBD. A cell proliferation assay demonstrated PUMA peptide inhibited the growth of a lung cancer cell line. These results contribute to understanding of the mechanism responsible for p53-mediated apoptosis.
Chang Woo Han, Han Na Lee, Mi Suk Jeong, So Young Park, Se Bok Jang

1010 related Products with: Structural basis of the p53 DNA binding domain and PUMA complex.

1000 TESTS/0.65ml100ul 100ul200 ug100 μg50ug96T100ug Lyophilized100ug 100ul100 ul

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#33631629   2021/02/06 To Up

Biospectroscopy and chemometrics as an analytical tool for comparing the antibacterial mechanism of silver nanoparticles with popular antibiotics against Escherichia coli.

Despite the fact that silver nanoparticles (AgNPs) have been widely studied in medical and correlated fields, details on their mechanisms are yet to be fully understood. Herein we present the first study on the combination of infrared spectroscopy and chemometrics as an analytical tool to investigate the mechanism of action of AgNPs against Escherichia coli by comparison with popular and commercially available antibiotics. The rationale behind this study is that the selected antibiotics act on bacteria in specific and distinct manners (DNA, cell membrane, mitochondria, etc.). Hence, via multivariate analysis we were able to compare the spectra of bacteria treated with the antibiotics and AgNPs to determine the main target of the latter. Spectral comparison, exploratory analysis, clustering and classification based on infrared spectra were carried out for E. coli samples in the absence and presence (treated) of four widely known antibiotics (ampicillin, ciprofloxacin, gentamicin and sulfadiazine) as well as RA-AgNPs and ERA-AgNPs. Chemometrics models indicated an interesting similarity between infrared spectra from E. coli treated with sulfadiazine and AgNPs, in which vibrational modes associated to phosphate groups were found to be the most representative. This result suggests that both AgNPs and sulfadiazine affects DNA structural features and availability, but not necessarily through the same mechanism. This biospectroscopy-based approach opens an interesting possibility for the understanding over the mechanism of antibacterial activity of AgNPs.
Ana C O Neves, Anderson D Viana, Fabrício G Menezes, Alcides O Wanderlei Neto, Maria Celeste N Melo, Luiz H S Gasparotto

1621 related Products with: Biospectroscopy and chemometrics as an analytical tool for comparing the antibacterial mechanism of silver nanoparticles with popular antibiotics against Escherichia coli.

96 Tests1 mL200 200 50 assays200 200 200 200 100 200 100

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#33631598   2021/02/22 To Up

Partial recovery of senescence in circulating follicular helper T cells after Dasatinib treatment.

Cellular senescence is an irreversible arrest of cell proliferation triggered by different stimuli, including DNA damage, telomere shortening and oncogenic stress. Senescent cells, by releasing the senescence-associated-secretory-phenotype (SASP), contribute to various diseases pathogenesis. Human atherosclerotic plaque contains cells with multiple markers of senescence that associate with disease severity. We characterized the frequency of senescent cTfh cells and genes expressions before and after treatment with Dasatinib in patients with different degrees of stenosis. Twelve high (≥50%), and twelve low (<50%) stenosis patients and six healthy controls were enrolled. The percentage of senescent CD3CD4CXCR5CD153CD57 cells was significantly decreased in Dasatinib treated cells from individuals with low and high stenosis (P = 0.0007 and P = 0.0002, respectively). However, the frequency of total lymphocytes, CD3 and CD4 T cells were not significantly different between the groups before and after treatment. The expression levels of P53 (P = 0.0003 and P = 0.0001), P16 (P = 0.0005 and P = 0.0002), p21 (P = 0.0002 and P < 0.0001), SENEX (P = 0.0005 and P < 0.0001) and BCL-2 (P = 0.0005 and P = 0.0002) were decreased in PBMCs of low and high stenosis groups after treatment with Dasatinib, respectively. The percentage of senescent cTfh cells positively correlated with cholesterol (P = 0.034; r = 0.671), C-reactive protein (CRP) (P = 0.029; r = 0.707), Erythrocyte sedimentation rate (ESR) levels (P = 0.030; r = 0.598) and neutrophil counts (P = 0.021; r = 0.799) in patients with high stenosis. The decreased frequency of senescent cTfh cells and the expression levels of senescence genes after Dasatinib treatment in patients with atherosclerosis suggest a role for Dasatinib in partial clearance or rejuvenation of senescent cTfh cells, which may decrease inflammatory mediators and attenuate disease progression.
Atefe Ghamar Talepoor, Shahdad Khosropanah, Mehrnoosh Doroudchi

2281 related Products with: Partial recovery of senescence in circulating follicular helper T cells after Dasatinib treatment.

96 tests96 tests96T50100 ug/vial

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#33631540   2021/02/22 To Up

Telomerase reverse transcriptase promoter mutation- and O-methylguanine DNA methyltransferase promoter methylation-mediated sensitivity to temozolomide in isocitrate dehydrogenase-wild-type glioblastoma: is there a link?

Benefit from temozolomide (TMZ) chemotherapy in the treatment of isocitrate dehydrogenase (IDH)-wild-type glioblastoma is essentially limited to patients with O-methylguanine DNA methyltransferase (MGMT) promoter-methylated tumours. Recent studies suggested that telomerase reverse transcriptase (TERT) promoter hotspot mutations may have an impact on the prognostic role of the MGMT status in patients with glioblastoma.
Dorothee Gramatzki, Jörg Felsberg, Bettina Hentschel, Marietta Wolter, Gabriele Schackert, Manfred Westphal, Luca Regli, Niklas Thon, Marcos Tatagiba, Wolfgang Wick, Uwe Schlegel, Dietmar Krex, Jakob Matschke, Patrick Roth, Marian P Suresh, Marcel A Kamp, Elisabeth J Rushing, Torsten Pietsch, Andreas von Deimling, Michael Sabel, Markus Loeffler, Michael Weller, Guido Reifenberger

2996 related Products with: Telomerase reverse transcriptase promoter mutation- and O-methylguanine DNA methyltransferase promoter methylation-mediated sensitivity to temozolomide in isocitrate dehydrogenase-wild-type glioblastoma: is there a link?

1 ml300 units100 assays100 ul100ug Lyophilized25 µg100ug Lyophilized100ug Lyophilized1mg0.1ml0.1 ml100ug Lyophilized

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#33631460   2021/02/08 To Up

Prediction of protein structural class based on symmetrical recurrence quantification analysis.

Protein structural class prediction for low similarity sequences is a significant challenge and one of the deeply explored subjects. This plays an important role in drug design, folding recognition of protein, functional analysis and several other biology applications. In this paper, we worked with two benchmark databases existing in the literature (1) 25PDB and (2) 1189 to apply our proposed method for predicting protein structural class. Initially, we transformed protein sequences into DNA sequences and then into binary sequences. Furthermore, we applied symmetrical recurrence quantification analysis (the new approach), where we got 8 features from each symmetry plot computation. Moreover, the machine learning algorithms such as Linear Discriminant Analysis (LDA), Random Forest (RF) and Support Vector Machine (SVM) are used. In addition, comparison was made to find the best classifier for protein structural class prediction. Results show that symmetrical recurrence quantification as feature extraction method with RF classifier outperformed existing methods with an overall accuracy of 100% without overfitting.
Ines Abdennaji, Mourad Zaied, Jean-Marc Girault

2352 related Products with: Prediction of protein structural class based on symmetrical recurrence quantification analysis.

96T0.2 mg96T1mg100ug1 Set220100ug Lyophilized1 Set

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