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#32783386   2020/08/12 To Up

Pentaplex real-time PCR for differential detection of Yersinia pestis and Y. pseudotuberculosis and application for testing fleas collected during plague epizootics.

Upon acquiring two unique plasmids (pMT1 and pPCP1) and genome rearrangement during the evolution from Yersinia pseudotuberculosis, the plague causative agent Y. pestis is closely related to Y. pseudotuberculosis genetically but became highly virulent. We developed a pentaplex real-time PCR assay that not only detects both Yersinia species but also differentiates Y. pestis strains regarding their plasmid profiles. The five targets used were Y. pestis-specific ypo2088, caf1, and pst located on the chromosome, plasmids pMT1 and pPCP1, respectively; Y. pseudotuberculosis-specific chromosomal gene opgG; and 18S ribosomal RNA gene as an internal control for flea DNA. All targets showed 100% specificity and high sensitivity with limits of detection ranging from 1 fg to 100 fg, with Y. pestis-specific pst as the most sensitive target. Using the assay, Y. pestis strains were differentiated 100% by their known plasmid profiles. Testing Y. pestis and Y. pseudotuberculosis-spiked flea DNA showed there is no interference from flea DNA on the amplification of targeted genes. Finally, we applied the assay for testing 102 fleas collected from prairie dog burrows where prairie dog die-off was reported months before flea collection. All flea DNA was amplified by 18S rRNA; no Y. pseudotuberculosis was detected; one flea was positive for all Y. pestis-specific targets, confirming local Y. pestis transmission. Our results indicated the assay is sensitive and specific for the detection and differentiation of Y. pestis and Y. pseudotuberculosis. The assay can be used in field investigations for the rapid identification of the plague causative agent.
Ying Bai, Vladimir Motin, Russell E Enscore, Lynn Osikowicz, Maria Rosales Rizzo, Andrias Hojgaard, Michael Kosoy, Rebecca J Eisen

1160 related Products with: Pentaplex real-time PCR for differential detection of Yersinia pestis and Y. pseudotuberculosis and application for testing fleas collected during plague epizootics.

100ul100 TESTS0.1 mg0.1 mg0.1 ml250 ml 100ul0.2 mg2x96 well plate1 LITRE25

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#32783325   2020/08/12 To Up

Histopathological evaluation of different regenerative protocols using Chitosan-based formulations for management of immature non-vital teeth with apical periodontitis: In vivo study.

This study aimed to assess regenerative treatment protocols for maturogenesis of immature teeth with apical periodontitis in dogs. Apical periodontitis was induced in immature premolars of 8 mongrel dogs teeth that were divided into 5 groups; regeneration via blood clotting (REG group); chitosan loaded with demineralised bone matrix (REG-CD group); chitosan loaded with dexamethazone corticosteroid (REG-CC group); and positive and negative control groups. All groups showed comparable apical hard tissue formation and significantly different from the control group. Results also showed decrease in inflammatory tissue reaction, bone resorption and periodontal ligament thickness. Tissue reaction and inflammatory infiltrates were significantly less in REG-CC group compared to others. Other parameters showed no significant difference. In conclusion, regenerative endodontic techniques using chitosan-based formulations have the potential to be used as an alternative for root maturation in teeth with apical periodontitis.
Khaled Farouk Abbas, Hossam Tawfik, Ahmed Abdel Rahman Hashem, Hany Mohamed Aly Ahmed, Ashraf Mohamed Abu-Seida, Hanan Mohamed Refai

2054 related Products with: Histopathological evaluation of different regenerative protocols using Chitosan-based formulations for management of immature non-vital teeth with apical periodontitis: In vivo study.

1 mg48 samples 5 G20 ug10 ug500 MG250 mg

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#32783227   2020/08/11 To Up

To live with a wagging tailed assistant - Service and hearing dog ownership from the perspective of Swedish owners.

Individuals who have a functional or health impairment, are often in great need of conventional healthcare, social care and support, as well as help from family and friends. The use of dogs may be an important assistive support for this population. Assistance dogs are trained to assist with their owners' specific needs. The aim of this study is to explore service and hearing dog ownership from the owner´s perspective, by examining the owner's expectations before training a dog, and experiences after having a certified dog. This study was designed as a longitudinal intervention study with pre-post design. The participants included in the study trained their own dogs to become service or hearing dogs. A number of open-ended questions were answered by the participants before the training of the dog started, three months after the dog was certified, and 1-3 years after the first follow-up. Data were analysed using thematic content analysis. The participants' expectations of what the dog would contribute after being certified was high. Their perceived experiences in many ways reflected their expectations. For example, they perceived that the dog contributed to improved health status, a more active lifestyle, improved ability to feel secure, and that they had become more independent. They also felt that the dog had strengthened their social relationships. Negative experiences were also identified. Not being allowed to bring their dog into public places and negative attitudes from other people were examples of this. This study shows that individuals being supported by an assistance dog experience the dog as an invaluable help in their everyday life. By improving the owners' lives in many ways, a certified service or hearing dog is a novel and important assistive support for people with a functional or health impairment.
Martina Lundqvist, Lars-Åke Levin, Jenny Alwin, Ann-Charlotte Nedlund

2889 related Products with: To live with a wagging tailed assistant - Service and hearing dog ownership from the perspective of Swedish owners.

600 Tests / Kit430 testsEach430 Tests / Kit100.00 ul1 mg1 ml

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#32783206   2020/08/12 To Up

Atlanto-axial ventral stabilisation using 3D-printed patient-specific drill guides for placement of bicortical screws in dogs.


C Toni, B Oxley, S Behr

1746 related Products with: Atlanto-axial ventral stabilisation using 3D-printed patient-specific drill guides for placement of bicortical screws in dogs.

4 Arrays/Slide10mg4 Membranes/Box2 Pieces/Box2.50 nmol4 Membranes/Box2 Pieces/Box100 ml4 Arrays/Slide2 Pieces/Box4 Membranes/Box2 Pieces/Box

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#32783156   2020/08/11 To Up

Who killed my dog? Use of forensic genetics to investigate an enigmatic case.

Genetic testing of animal biological material has become a valuable tool in forensic investigations, and it is successfully used to identify unknown crime perpetrators, to unmask food frauds, or to clarify cases of animal attacks on humans or other animals. When DNA profiling is not possible due to inadequate amounts of nuclear DNA, mitochondrial DNA (mtDNA) testing is the only viable alternative, as in the case of shed hair samples. In this case, a dog was allegedly killed by wild animals while being hosted in a boarding house. Extraneous hair fragments recovered from the dog's mouth and paws were subjected to genetic analysis: the cytochrome b gene located on mtDNA was amplified and sequenced in order to determine the species responsible for the killing. The mtDNA analysis provided evidence that the dog was killed by other dogs, thus unmasking a false wild animal attack and putting the case in an entirely different perspective.
Mariana Roccaro, Carla Bini, Paolo Fais, Giuseppe Merialdi, Susi Pelotti, Angelo Peli

2930 related Products with: Who killed my dog? Use of forensic genetics to investigate an enigmatic case.

2 modules200 ug100ug Lyophilized100 ug100ug1 Set200 0.025 mg0.1ml (1.3mg/ml)100ug1 mg1 module

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#32783071   2020/08/12 To Up

Morphological and molecular characterization of Cystoisospora laidlawi oocysts (Apicomplexa: Eimeriidae) in farmed American mink (Neovison vison) in Denmark.

From a longitudinal survey conducted on 30 Danish mink farms in 2016, 11.0% of faecal samples (456/4140) were positive for Cystoisospora laidlawi oocysts by microscopy, with 60% (189/315) of mink being positive at least once during the study period. Morphological analysis of sporulated oocysts identified Cystoisospora oocysts measuring 34.3 × 29.5 μm with an oocyst length/width (L/W) ratio of 1.2. The morphological features of the oocysts were identical to Isospora laidlawi previously morphological identified in farmed mink from Denmark and elsewhere. Phylogenetic analysis of 18S rDNA sequences (1221 bp) from three positive mink indicated that Cystoisospora from mink shared the highest genetic similarity to C. canis from a Canadian dog (99.6%). The phylogenetic analysis placed Cystoisospora from mink in a clade with other Cystoisospora isolates.
H H Petersen, R Yang, M Chriel, D Liu, M S Hansen, U M Ryan

2968 related Products with: Morphological and molecular characterization of Cystoisospora laidlawi oocysts (Apicomplexa: Eimeriidae) in farmed American mink (Neovison vison) in Denmark.

1 mL25mg100 μg100ug100 μg100ug Lyophilized5 g100ug Lyophilized 100 G

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#32782751   2020/06/15 To Up

Concurrent occurrence of granulosa cell tumor, uterine adenomyosis, cystic endometrial hyperplasia and uterine serosal inclusion cyst in a bitch.

An eleven-year-old Doberman-German Shepherd mixed bitch was presented with vulvar enlargement, vaginal discharge, and bleeding for two months and a week before, respectively. The dog was operated for routine ovariohysterectomy. During the surgery, a liquid-filled sac was observed on the ventrolateral border of the right uterine horn. The sac was ligated and excised. The uterine horns and ovaries were stained for histopathological evaluation. Granulosa cell tumor (GCT), adenomyosis (CUA), endometrial cystic hyperplasia (CEH), and uterine serosal inclusion cyst (USC) were diagnosed through histopathology assessment. Following up, 12 weeks after surgery revealed that the patient was in good condition with a good appetite and without any vaginal discharge. It seems the initial problem of the present bitch and the main cause of clinical signs was GCT. Probably, other abnormal conditions including CUA, CEH, and USC occurred as a result of hormonal disturbance associated with GCT. This report described the concurrent occurrence of GCT, CUA, CEH, and USC in a bitch as it has not been reported before.
Soroush Sabiza, Annahita Rezaie, Reza Avizeh, Navid Razmian, Reza Samaei, Ali Aghababaei

2926 related Products with: Concurrent occurrence of granulosa cell tumor, uterine adenomyosis, cystic endometrial hyperplasia and uterine serosal inclusion cyst in a bitch.



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#32782749   2020/06/15 To Up

A cross sectional study on and in sheepdogs in a western region in Iran.

Iran is one of the endemic areas of Dirofilariasis, and also one of the most important zoonotic infections. causes a severe and fatal disease called heartworm disease in dog. It also produces pulmonary nodules in humans. The worm is to be investigated as a potential infection of humans and animals in various provinces in Iran. In this research, the samples were studied with modified Knott's test and molecular method. The results of the modified Knott's test method indicated that 14.00% of sheepdogs were infected with filarial microfiler. The microfilers were characterized with basic morphological features, the length of the infective larva and tail ending. There was an estimated prevalence of 4.45% for and 9.55% for microfiler. To verify the differential diagnosis, molecular method was performed using PCR with specific primers for amplification of ITS2 locus. Gene locus sequencing results of and sequence alignment recorded in GeneBank showed 97.00% similarity, and relatively 98.00% similarity was observed in The results of the molecular method confirmed the result of modified Knott's test method. Low infection with was observed the region, probably due to the fact that the annual temperature and precipitation in this area were not suitable for the proliferation of the vector mosquitoes. In general, there was less infection in the region compared to regions with relatively similar climatic conditions. Hence, the results suggested that alternative diagnostic tests are required to determine the occult infections.
Mandana Hoseini, Fateme Jalousian, Seyed Hosein Hoseini, Abbas Gerami Sadeghian

2778 related Products with: A cross sectional study on and in sheepdogs in a western region in Iran.

100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg

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#32782138   2020/06/20 To Up

Molecular cloning, sequence analysis, and tissue distribution of marmoset monoamine oxidases A and B.

The common marmoset (Callithrix jacchus), a New World primate, is currently attracting much attention as a nonhuman primate model for pharmacological and pharmacokinetic studies in preclinical research. In this study, we newly isolated the cDNAs of marmoset monoamine oxidase A (MAO-A) and MAO-B from liver and brain, respectively. MAO-A and MAO-B cDNAs, respectively, contained open reading frames of 527 and 520 amino acids and were approximately 92% and 95% identical to their human orthologs. Marmoset MAOs were phylogenetically closer to primate MAOs, including human MAOs, than to pig, dog, or rodent MAOs. The genomic and gene structures of marmoset MAOs were similar to those of humans. Among the five marmoset tissue types analyzed, the expression levels of MAO-A mRNA were relatively abundant in lung, liver, kidney, and small intestine, whereas the expression levels of MAO-B mRNA were relatively abundant in brain, liver, kidney, and small intestine; these tissue distributions are similar to those of human MAOs. These results suggest that MAO-A and MAO-B are similar at a molecular level in marmosets and humans.
Shotaro Uehara, Yasuhiro Uno, Hiroshi Yamazaki

2452 related Products with: Molecular cloning, sequence analysis, and tissue distribution of marmoset monoamine oxidases A and B.

25 mg1000 tests100ul200ul100 mg10 mg200ug10 mg50 ug 100ug

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#32781793   2020/08/06 To Up

Excretory/Secretory Metabolome of the Zoonotic Roundworm Parasite .

Toxocariasis is a zoonotic disease affecting humans that is predominantly caused by and , primarily parasites of dogs and cats, respectively. generally establishes long-term infections by co-opting its host's physiological processes, while at the same time exploiting the nutritional environment. Adult stage reside in the gut of the definitive canine host where they employ a suite of strategies to combat intestinal immune responses by actively producing and releasing excretory-secretory products (ESPs). The protein component of ESPs has been widely studied, but characterisation of the non-protein ESP complement remains neglected. To characterize the secreted metabolome of ESPs and to shed light on the parasite's metabolic processes, we profiled the ESPs of using both gas chromatography (GC) and liquid chromatography (LC) mass spectrometry approaches. We successfully identified 61 small molecules, including 41 polar metabolites, 14 medium-long chain fatty acids (MLCFAs) and six short chain fatty acids (SCFAs). We identified talose, stearic acid and isovalerate as the major compounds belonging to the polar, MLCFA and SCFA chemical classes, respectively. Most of the 61 identified metabolites appear to have been produced by via three distinct metabolic pathways - fatty acid, amino acid and carbohydrate metabolism. The majority of the identified ESPs have known biological properties, especially as immunomodulators. However, there is limited/no information on the biological roles or applications of 31 ESP biomolecules, suggesting that these may have novel activities that merit further investigation.
Phurpa Wangchuk, Owen Lavers, David S Wishart, Alex Loukas

2452 related Products with: Excretory/Secretory Metabolome of the Zoonotic Roundworm Parasite .

500 Units100ug Lyophilized1500100ug Lyophilized1mg200 12000 IU100ug Lyophilized

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