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#34525457   2021/09/15 To Up

Medical management of heavily exposed victims: An experience at the Tokaimura criticality accident.

A criticality accident occurred at the uranium conversion plant in Tokaimura, Ibaraki Prefecture, Japan on September 30, 1999. When uranyl nitrate was overloaded to a critical mass level, uncontrolled fission reaction occurred. A procedure was carried out according to the JCO manual, although not an officially approved manual. Three workers were heavily exposed to neutrons and -rays produced by nuclear fission, and they subsequently developed acute radiation syndrome (ARS). The average doses to the whole body of the three workers were approximately 25, 9, and 3 GyEq (biologically equivalent dose of γ-exposure), respectively; dose distribution analysis later revealed extreme heterogeneity of these doses in 2 workers. They were triaged according to the predicted clinical needs. Two of these workers developed severe bone marrow failure and received hematopoietic stem cell transplantation: one with peripheral stem cell transplantation from his HLA compatible sister and the other with umbilical cord blood transplantation. The graft was initially successful in both workers; autologous hematopoietic recovery was observed after donor/recipient mixed chimerism in one of them. Despite of all medical efforts available including hematopoietic stem cell transplantation, investigational drugs, skin graft, two workers died of multiple organ involvement and failure 83 and 211 days after the accident, respectively. Clinically as well as pathologically, the direct cause of death was deemed to be intractable GI bleeding in one, and thoraco-abdominal compartment syndrome due to dermal fibrosis/sclerosis in the other. The third worker also developed bone marrow suppression but was treated with granulocyte colony-stimulating factor (G-CSF). He recovered without major complications and is now under periodical medical follow-up. These experiences suggest that treatment of bone marrow is not a limiting factor for saving the life of ARS victims severely exposed. Successful treatment of other organs such as lungs, skin, and gastrointestinal tract is also essential. Furthermore, the whole-body dose may not always reflect the prognosis of ARS victims because of the nature of accidental exposure, heterogenous exposure.
Makoto Akashi, Kazuhiko Maekawa

2318 related Products with: Medical management of heavily exposed victims: An experience at the Tokaimura criticality accident.

100μl 100ul50 ug 100 ul100ug Lyophilized100ug1 ml100ul1 Set8 Sample Kit100ug Lyophilized

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#34525329   2021/09/12 To Up

Application of carbon monoxide in kidney and heart transplantation: A novel pharmacological strategy for a broader use of suboptimal renal and cardiac grafts.

Carbon monoxide (CO) was historically regarded solely as a poisonous gas that binds to hemoglobin and reduces oxygen-carrying capacity of blood at high concentrations. However, recent findings show that it is endogenously produced in mammalian cells as a by-product of heme degradation by heme oxygenase, and has received a significant attention as a medical gas that influences a myriad of physiological and pathological processes. At low physiological concentrations, CO exhibits several therapeutic properties including antioxidant, anti-inflammatory, anti-apoptotic, anti-fibrotic, anti-thrombotic, anti-proliferative and vasodilatory properties, making it a candidate molecule that could protect organs in various pathological conditions including cold ischemia-reperfusion injury (IRI) in kidney and heart transplantation. Cold IRI is a well-recognized and complicated cascade of interconnected pathological pathways that poses a significant barrier to successful outcomes after kidney and heart transplantation. A substantial body of preclinical evidence demonstrates that CO gas and CO-releasing molecules (CO-RMs) prevent cold IRI in renal and cardiac grafts through several molecular and cellular mechanisms. In this review, we discuss recent advances in research involving the use of CO as a novel pharmacological strategy to attenuate cold IRI in preclinical models of kidney and heart transplantation through its administration to the organ donor prior to organ procurement or delivery into organ preservation solution during cold storage and to the organ recipient during reperfusion and after transplantation. We also discuss the underlying molecular mechanisms of cyto- and organ protection by CO during transplantation, and suggest its clinical use in the near future to improve long-term transplantation outcomes.
George J Dugbartey, Karl K Alornyo, Patrick P W Luke, Alp Sener

1087 related Products with: Application of carbon monoxide in kidney and heart transplantation: A novel pharmacological strategy for a broader use of suboptimal renal and cardiac grafts.

100ul100 ml50ul1 ml 25 MG 100ul200ug25 mg100ug Lyophilized

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#34525263   2021/09/15 To Up

Cytomegalovirus mismatch after heart transplantation: Impact of antiviral prophylaxis and intravenous hyperimmune globulin.

Cytomegalovirus (CMV) infections are correlated with complications following heart transplantation (HTx) and impaired outcome. The impact of a serologic mismatch between donor and recipient and the necessity of prophylactic virostatic medication is still a matter of concern.
Moritz B Immohr, Payam Akhyari, Charlotte Böttger, Arash Mehdiani, Hannan Dalyanoglu, Ralf Westenfeld, Daniel Oehler, Igor Tudorache, Hug Aubin, Artur Lichtenberg, Udo Boeken

1021 related Products with: Cytomegalovirus mismatch after heart transplantation: Impact of antiviral prophylaxis and intravenous hyperimmune globulin.

1 mL25 mg50 µg 100ul1000 tests100ug100ug10 mg100 5 G

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#34525148   2021/09/15 To Up

The n-type and p-type conductivity mechanisms of the bulk BiOCl photocatalyst from hybrid density functional theory calculations.

In this work, the n-type and p-type conductivity mechanisms of bulk BiOCl are systematically investigated using first-principles calculations. Under the O-rich growth conditions, BiOCl presents the intrinsic p-type conductivity, which primarily originates from the contributions of the antisite defects O. This is in excellent agreement with the observed p-type conductivity in BiOCl under high oxygen partial pressure or in oxygen-saturated solutions in experiments. While BiOCl displays the intrinsic n-type conductivity under the Bi-rich growth conditions, the vacancy defects V are responsible for the character. Nevertheless, the extrinsic means or effects could lead to the production and ionization of the O vacancies, which could contribute to the n-type conductivity in BiOCl. Therefore, the intrinsic n-type conductivity behavior reported in BiOCl in recent experiments is well explained. Under the Cl-rich growth conditions, the major donor defects V and the major acceptor defects V in BiOCl compensate each other, leading to an intrinsic insulator. However, it is shown that the usual unintentional H impurities H could form the p-type conductivity in BiOCl under Cl-rich growth conditions and enhance the p-type conductivity under O-rich growth conditions. This helps to understand the p-type conductivity behavior reported or mentioned in BiOCl in many experiments. At the same time, we found that Group II elements Ca and Sr are superior p-type doping elements for BiOCl under the Cl-rich and O-rich growth conditions. The defect physics in the bulk BiOCl photocatalyst is well understood. This work may inspire more magnificent studies on developing BiOCl-based photocatalysts.
Bo Kong, Tixian Zeng, Wentao Wang

2218 related Products with: The n-type and p-type conductivity mechanisms of the bulk BiOCl photocatalyst from hybrid density functional theory calculations.

96T10.5 mg0.5 mg

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#34524912   2021/09/15 To Up

A cGAS-dependent response links DNA damage and senescence in alveolar epithelial cells: A potential drug target in IPF.

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Michael Schuliga, Amama Kanwal, Jane Read, Kaj Erik Cornelis Blokland, Janette K Burgess, Cecilia M Prêle, Steven E Mutsaers, Christopher Grainge, Claire Thomson, Allen James, Nathan W Bartlett, Darryl A Knight

1750 related Products with: A cGAS-dependent response links DNA damage and senescence in alveolar epithelial cells: A potential drug target in IPF.

0.1ml100 assays0.1ml100 assays0.1ml (1mg/ml)100 assays100 assays1mg0.1ml100 assays96 assays100 assays

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#34524900   2021/09/15 To Up

Targeted gene insertion and replacement in the basidiomycete by inactivation of non-homologous end joining using CRISPR/Cas9.

Targeted gene insertion or replacement is a promising genome editing tool for molecular breeding and gene engineering. Although CRISPR/Cas9 works well for gene disruption and deletion in , targeted gene insertion and replacement remains a serious challenge due to the low efficiency of homologous recombination (HR) in these species. In this work, we demonstrate that the DNA double-strand breaks induced by Cas9 were mainly repaired via the non-homologous end joining pathway (NHEJ) at a frequency of 96.7%. To establish an efficient target gene insertion and replacement tool in , we first inactivated the NHEJ pathway via disruption of the Ku70 gene () using a dual sgRNA-directed gene deletion method. Disruption of the significantly decreased NHEJ activity in . Moreover, disruption strains exhibited 96.3% and 93.1% frequencies of a targeted gene insertion and replacement when target DNA orotidine 5'-monophosphate decarboxylase gene () with 1.5 kb 5' and 3' homologous flanking sequences were used as a donor template, compared to 3.3% and 0% for a control strain (Cas9 strain) at these targeted sites, respectively. Our results indicated that disruption strains were efficient recipients for targeted gene insertion and replacement. This tool will advance our understanding of functional genomics in . Functional genomic studies have been hindered in by the absence of adequate genome engineering tools. Although CRISPR/Cas9 works well for gene disruption and deletion in , targeted gene insertion and replacement has remained a serious challenge due to the low efficiency of homologous recombination in these species, although such precise genome modifications including site mutations, site-specific integrations and allele or promoter replacements would be incredibly valuable. In this work, we inactivated the non-homologous end joining repair mechanism in by disrupting the using the CRISPR/Cas9 system. Moreover, we established a target gene insertion and replacement method in -disrupted that possessed high-efficiency gene targeting. This technology will advance our understanding of the functional genomics of
Jun-Liang Tu, Xin-Yuan Bai, Yong-Liang Xu, Na Li, Jun-Wei Xu

1663 related Products with: Targeted gene insertion and replacement in the basidiomycete by inactivation of non-homologous end joining using CRISPR/Cas9.

300 units100 μg96T1.00 flask96T1.00 flask

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#34524862   2021/09/15 To Up

Static lung storage at 10°C maintains mitochondrial health and preserves donor organ function.

[Figure: see text].
Aadil Ali, Aizhou Wang, Rafaela V P Ribeiro, Erika L Beroncal, Cristina Baciu, Marcos Galasso, Bruno Gomes, Andrea Mariscal, Olivia Hough, Edson Brambate, Etienne Abdelnour-Berchtold, Vinicius Michaelsen, Yu Zhang, Anajara Gazzalle, Eddy Fan, Laurent Brochard, Jonathan Yeung, Tom Waddell, Mingyao Liu, Ana C Andreazza, Shaf Keshavjee, Marcelo Cypel

2515 related Products with: Static lung storage at 10°C maintains mitochondrial health and preserves donor organ function.

100ug Lyophilized100 mg100ug Lyophilized

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#34524821   2021/09/15 To Up

Tracking Ultrafast Fluorescence Switch-On and Color-Tuned Dynamics in Acceptor-Donor-Acceptor Chromophore.

Understanding how the conformational change of conjugated molecules with acceptor-donor-acceptor (A-D-A) architecture affects their physical and optoelectronic properties is critical for determining their ultimate performance in organic electronic devices. Here, we utilized femtosecond transient absorption, time-resolved upconversion photoluminescence spectroscopy, and tunable femtosecond-stimulated Raman spectroscopy, aided by quantum chemical calculations, to systematically investigate the excited state structural dynamics of the intramolecular charge transfer of the tetramethoxy anthracene-based fluorophore 2,3,6,7-tetramethoxy 9,10-dibenzaldehydeanthracene (AnDA) and its derivative 2,3,6,7-tetramethoxy 9,10-diphenylanthracene (TMDPAn) in chloroform. In the AnDA molecule, the tetramethoxy anthracene and benzaldehyde moieties exhibit a strong ability to donate and withdraw electrons. Upon photoexcitation, AnDA shows intriguing ultrafast fluorescence switch-on and red shift dynamics on charge transfer states, and the temporal evolution of AnDA recorded by ultrafast spectroscopy reveals a dynamic picture of two-step intramolecular charge transfer assisted by ultrafast conformational changes and solvation processes. Removing the aldehyde group from TMDPAn significantly decreases the electron pulling capacity of the phenyl unit and disables charge transfer characteristics.
Wenqi Xu, Lei Wei, Zhengxin Wang, Ruixue Zhu, Jiaming Jiang, Huiyan Liu, Juan Du, Tsu-Chien Weng, Yue-Biao Zhang, Yifan Huang, Weimin Liu

1008 related Products with: Tracking Ultrafast Fluorescence Switch-On and Color-Tuned Dynamics in Acceptor-Donor-Acceptor Chromophore.

1 kit100ug480/kit1 kit1 kit1 kit1 kit1 kit1 kit1 kit

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#34524814   2021/09/15 To Up

A Fast-Responsive OFF-ON Near-Infrared-II Fluorescent Probe for In Vivo Detection of Hypochlorous Acid in Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a common chronic autoimmune inflammatory disease, and its etiology is closely related to the overproduction of hypochlorous acid (HClO). However, early detection of RA using an activatable near-infrared-II (NIR-II, 1000-1700 nm) fluorescent probe remains challenging. Herein, we first report an "OFF-ON" NIR-II fluorescent probe named (phenothiazine triphenylamine) for imaging HClO in deep-seated early RA. Electron-rich phenothiazine in the core of was utilized as both an HClO-recognition moiety and a precursor of electron acceptors, displaying a typical donor-acceptor-donor structure with excellent NIR-II emission at 936/1237 nm once reacted with HClO. The probe exhibited good water solubility, high photostability, and rapid response capability toward HClO within 30 s. Moreover, it was able to sensitively and specifically detect exogenous and endogenous HClO in living cells in both visible and NIR-II windows. Notably, enabled the sensitive and rapid visualization of HClO generation in an inflammatory RA mouse model, showing a 4.3-fold higher NIR-II fluorescence intensity than that in normal hindlimb joints. These results demonstrate that holds great promise as a robust platform for diagnosis of HOCl-mediated inflammatory disorders.
Peng Wu, Yu Zhu, Lulu Chen, Yang Tian, Hu Xiong

2450 related Products with: A Fast-Responsive OFF-ON Near-Infrared-II Fluorescent Probe for In Vivo Detection of Hypochlorous Acid in Rheumatoid Arthritis.

25 G100ug Lyophilized1 g2.5 mg25 mg 1 G100ug Lyophilized100ug Lyophilized

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#34524666   // To Up

iNKT Cel Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach.

Natural killer T (NKT) cells are an innate-like T cell subset that recognize lipid antigens presented by CD1d-expressing antigen presenting cells (APCs), such as dendritic cells, macrophages, and B cells. They can be subdivided into two different subsets according to the variation in αβ TCR chains: type I and type II NKT cells. Type I, also called invariant NKT cells (iNKT), express restricted TCRs with an invariant α-chain (Vα24-Jα18 in humans and Vα14-Jα18 in mice) and limited β-chains. Here we have established a protocol in which iNKT cells are isolated from a donor wild-type mouse and transferred into iNKT KO (Jα18) mouse. Below we will explore the methods for cell sorting of splenic iNKTs, iNKT cells transfer, and detection of transferred cells into the liver using flow cytometry technique.
Marcella Cipelli, Theresa Ramalho, Cristhiane Favero de Aguiar, Niels Olsen Saraiva Camara

1327 related Products with: iNKT Cel Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach.

1 kit1 kit1 kit1 kit1 kit1 kit1 kit1 kit1 kit1 kit1 kit1 kit

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